JP1668257S - Sample separation device - Google Patents
Sample separation deviceInfo
- Publication number
- JP1668257S JP1668257S JP2019025791F JP2019025791F JP1668257S JP 1668257 S JP1668257 S JP 1668257S JP 2019025791 F JP2019025791 F JP 2019025791F JP 2019025791 F JP2019025791 F JP 2019025791F JP 1668257 S JP1668257 S JP 1668257S
- Authority
- JP
- Japan
- Prior art keywords
- storage section
- liquid
- sample
- microparticles
- collected
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000926 separation method Methods 0.000 title abstract 4
- 239000007788 liquid Substances 0.000 abstract 16
- 239000011859 microparticle Substances 0.000 abstract 7
- 238000001514 detection method Methods 0.000 abstract 2
- 239000002245 particle Substances 0.000 abstract 2
- 239000002699 waste material Substances 0.000 abstract 2
- 230000005284 excitation Effects 0.000 abstract 1
- 238000000684 flow cytometry Methods 0.000 abstract 1
- 239000011325 microbead Substances 0.000 abstract 1
- 230000003287 optical effect Effects 0.000 abstract 1
Abstract
本物品は、細胞、マイクロビーズ等の微小粒子の分取を行うフローサイトメトリーに用いる、使い捨て可能なデバイスである。当該デバイスは、閉鎖型セルソータの装置上にセットして用いられるものであり、各部位が密閉連結され、これらが一体不可分で用いられることにより、外気に接することなく目的とする回収されるべき微小粒子の分取を行うことができる。当該デバイスは、各部位を示す参考正面図に示すように、マイクロ流路チップ100の他に、サンプル収容部201、プレサンプル収容部2011、フィルタ部202、目標サンプル貯留部203、廃棄部204、シース液収容部205、ダンパー207、及び圧力計センサ208を備えており、これら各部位はチューブによって連結されている。当該デバイスにおいて、プレサンプル収容部2011に導入された微小粒子を含む液体(サンプル液)はフィルタ部202を介してサンプル収容部201に移動し、マイクロ流路チップ100内に導入され、回収されるべき微小粒子とそうでない微小粒子とに分取される。当該マイクロ流路チップ100は、マイクロ流路チップ部分の各部位の名称を示す参考正面図及び参考背面図に示すように、サンプル液インレット、シース液インレット、分取流路の末端、及び分岐流路の末端を同一側面に有しており、前記同一側面側(チューブが挿入された側)にはチューブガードが備えられ、導入されたサンプル液及びシース液が流れる主流路の一部には光学検出領域が備えられ、回収されるべき微小粒子が通流する分取流路の一部にはアクチュエータにより加振される加振領域が備えられている。前記マイクロ流路チップでは、サンプル液収容部201と連結したサンプル液インレットから導入されたサンプル液を、シース液収容部205と連結したシース液インレットから導入されたシース液によって液送し、前記光学検出領域にて微小粒子を検出した後、回収されるべき微小粒子含む液体はアクチュエータにより加振領域に対して負圧を発生させることで分取流路へ分取され、目標サンプル貯留部203へと移送される。また、回収されるべきでない微小粒子を含む液体は、分岐流路を経て排出され、廃棄部204へと移送される。なお、前記デバイスは、板状構造体の中にダンパー207及び圧力計センサ208も備えており、これらは送液による圧力をなるべく一定にするために設けられている。This article is a disposable device used for flow cytometry to separate microparticles such as cells and microbeads. The device is used by being set on a closed-type cell sorter, and each part is hermetically connected and is used as an inseparable unit, allowing the target microscopic particles to be collected without coming into contact with the outside air. Particle separation can be performed. As shown in the reference front view showing each part, the device includes, in addition to the microchannel chip 100, a sample storage section 201, a pre-sample storage section 2011, a filter section 202, a target sample storage section 203, a waste section 204, It includes a sheath liquid storage section 205, a damper 207, and a pressure gauge sensor 208, and these parts are connected by a tube. In this device, a liquid (sample liquid) containing microparticles introduced into a pre-sample storage section 2011 moves to the sample storage section 201 via a filter section 202, is introduced into the microchannel chip 100, and is collected. The microparticles are separated into those that are suitable and those that are not. The microchannel chip 100 has a sample liquid inlet, a sheath liquid inlet, an end of the separation channel, and a branch flow, as shown in the reference front view and reference rear view showing the names of each part of the microchannel chip. The ends of the channels are on the same side, a tube guard is provided on the same side (the side where the tube is inserted), and a part of the main channel through which the introduced sample liquid and sheath liquid flow is equipped with an optical A detection region is provided, and a part of the separation channel through which microparticles to be collected flow is provided with an excitation region that is vibrated by an actuator. In the microchannel chip, the sample liquid introduced from the sample liquid inlet connected to the sample liquid storage section 201 is transported by the sheath liquid introduced from the sheath liquid inlet connected to the sheath liquid storage section 205, and After detecting microparticles in the detection region, the liquid containing the microparticles to be collected is fractionated into the preparative flow path by generating negative pressure in the vibration region using an actuator, and then transferred to the target sample storage section 203. and will be transferred. Further, liquid containing microparticles that should not be collected is discharged through the branch flow path and transferred to the waste section 204. Note that the device also includes a damper 207 and a pressure gauge sensor 208 in the plate-like structure, and these are provided to keep the pressure caused by liquid feeding as constant as possible.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2019025791F JP1668257S (en) | 2019-11-20 | 2019-11-20 | Sample separation device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2019025791F JP1668257S (en) | 2019-11-20 | 2019-11-20 | Sample separation device |
Publications (1)
Publication Number | Publication Date |
---|---|
JP1668257S true JP1668257S (en) | 2020-09-14 |
Family
ID=87934954
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2019025791F Active JP1668257S (en) | 2019-11-20 | 2019-11-20 | Sample separation device |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP1668257S (en) |
-
2019
- 2019-11-20 JP JP2019025791F patent/JP1668257S/en active Active
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