IT201900024448A1 - Conservazione di sequenze di acidi nucleici mediante fissazione dei tessuti in formalina tamponata preparata utilizzando formaldeide deprivata di acidi - Google Patents

Conservazione di sequenze di acidi nucleici mediante fissazione dei tessuti in formalina tamponata preparata utilizzando formaldeide deprivata di acidi

Info

Publication number
IT201900024448A1
IT201900024448A1 IT102019000024448A IT201900024448A IT201900024448A1 IT 201900024448 A1 IT201900024448 A1 IT 201900024448A1 IT 102019000024448 A IT102019000024448 A IT 102019000024448A IT 201900024448 A IT201900024448 A IT 201900024448A IT 201900024448 A1 IT201900024448 A1 IT 201900024448A1
Authority
IT
Italy
Prior art keywords
deprivated
conservation
formaldehyde
nucleic acid
buffered formalin
Prior art date
Application number
IT102019000024448A
Other languages
English (en)
Inventor
Giovanni Bussolati
Benedetta Bussolati
Nicolò Bussolati
Original Assignee
Addax Biosciences S R L
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Addax Biosciences S R L filed Critical Addax Biosciences S R L
Priority to IT102019000024448A priority Critical patent/IT201900024448A1/it
Priority to US17/757,463 priority patent/US20230017439A1/en
Priority to CN202080088369.6A priority patent/CN114901067A/zh
Priority to CA3162334A priority patent/CA3162334A1/en
Priority to JP2022537616A priority patent/JP2023507440A/ja
Priority to EP20830140.8A priority patent/EP4075970A1/en
Priority to PCT/EP2020/086247 priority patent/WO2021122613A1/en
Publication of IT201900024448A1 publication Critical patent/IT201900024448A1/it

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2001/305—Fixative compositions
IT102019000024448A 2019-12-18 2019-12-18 Conservazione di sequenze di acidi nucleici mediante fissazione dei tessuti in formalina tamponata preparata utilizzando formaldeide deprivata di acidi IT201900024448A1 (it)

Priority Applications (7)

Application Number Priority Date Filing Date Title
IT102019000024448A IT201900024448A1 (it) 2019-12-18 2019-12-18 Conservazione di sequenze di acidi nucleici mediante fissazione dei tessuti in formalina tamponata preparata utilizzando formaldeide deprivata di acidi
US17/757,463 US20230017439A1 (en) 2019-12-18 2020-12-15 Preservation of nucleic acid sequences by fixing tissues in buffered formalin prepared with acid-deprived formaldehyde
CN202080088369.6A CN114901067A (zh) 2019-12-18 2020-12-15 通过在用去酸甲醛制备的缓冲福尔马林中固定组织来保存核酸序列
CA3162334A CA3162334A1 (en) 2019-12-18 2020-12-15 Preservation of nucleic acid sequences by fixing tissues in buffered formalin prepared with acid-deprived formaldehyde
JP2022537616A JP2023507440A (ja) 2019-12-18 2020-12-15 酸除去ホルムアルデヒドで調製した緩衝化ホルマリンの組織固定による核酸配列の保存
EP20830140.8A EP4075970A1 (en) 2019-12-18 2020-12-15 Preservation of nucleic acid sequences by fixing tissues in buffered formalin prepared with acid-deprived formaldehyde
PCT/EP2020/086247 WO2021122613A1 (en) 2019-12-18 2020-12-15 Preservation of nucleic acid sequences by fixing tissues in buffered formalin prepared with acid-deprived formaldehyde

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT102019000024448A IT201900024448A1 (it) 2019-12-18 2019-12-18 Conservazione di sequenze di acidi nucleici mediante fissazione dei tessuti in formalina tamponata preparata utilizzando formaldeide deprivata di acidi

Publications (1)

Publication Number Publication Date
IT201900024448A1 true IT201900024448A1 (it) 2021-06-18

Family

ID=70155111

Family Applications (1)

Application Number Title Priority Date Filing Date
IT102019000024448A IT201900024448A1 (it) 2019-12-18 2019-12-18 Conservazione di sequenze di acidi nucleici mediante fissazione dei tessuti in formalina tamponata preparata utilizzando formaldeide deprivata di acidi

Country Status (7)

Country Link
US (1) US20230017439A1 (it)
EP (1) EP4075970A1 (it)
JP (1) JP2023507440A (it)
CN (1) CN114901067A (it)
CA (1) CA3162334A1 (it)
IT (1) IT201900024448A1 (it)
WO (1) WO2021122613A1 (it)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3416482A1 (en) * 2016-02-18 2018-12-26 Addax Biosciences S.r.l. Acid free glyoxal as fixative for histological preparations

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1231836A4 (en) * 1999-11-17 2004-06-02 Univ Rochester EX VIVO HUMAN IMMUNE SYSTEM
WO2008131973A2 (en) * 2007-04-26 2008-11-06 Medinnova As Transplant storage
KR101822662B1 (ko) * 2009-02-04 2018-01-26 예일 유니버시티 폐 조직 엔지니어링
EP4008786A3 (en) * 2011-09-06 2022-08-03 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. The mirna-212/132 family as a therapeutic target
GB201303666D0 (en) * 2013-03-01 2013-04-17 Goldsborough Andrew S Sample fixation and stabilisation
CN108358768A (zh) * 2018-01-13 2018-08-03 安徽金禾实业股份有限公司 一种超低酸甲醛的生产方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3416482A1 (en) * 2016-02-18 2018-12-26 Addax Biosciences S.r.l. Acid free glyoxal as fixative for histological preparations

Non-Patent Citations (20)

* Cited by examiner, † Cited by third party
Title
AMEMIYA K ET AL.: "relationship between formalin reagent and success rate of targeted sequencing analysis using formalin fixed paraffin embedded tissue", CLINICAL CHEMISTRY ACTA, vol. 488, 2019, pages 129 - 134, XP085555573, DOI: 10.1016/j.cca.2018.11.002
BUSSOLATI G. ET AL.: "Formalin fixation at low temperature better preserves nucleic acid integrity", PLOS ONE, vol. 6, no. 6, 2011, pages e21043
DEAN L. OWENS: "Acid removal", 30 June 1980 (1980-06-30), XP055720312, Retrieved from the Internet <URL:https://p2infohouse.org/ref/27/26584.pdf> [retrieved on 20200805] *
DOTTI ET AL.: "effects of formalin, metacarn, and fineFIX fixatives on RNA preservation", DIAGN MOT PATHOL, vol. 19, no. 2, June 2010 (2010-06-01), pages 112 - 122
FOX ET AL.: "Formaldehyde fixation", JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, vol. 33, 1985, pages 845 - 853
GOGIASHVILI MIKHEIL ET AL: "Metabolic profiling ofob/obmouse fatty liver using HR-MAS1H-NMR combined with gene expression analysis reveals alterations in betaine metabolism and the transsulfuration pathway", CORESTA PTM TECHNICAL REPORT, SPRINGER BERLIN HEIDELBERG, DE, vol. 409, no. 6, 28 November 2016 (2016-11-28), pages 1591 - 1606, XP036153775, ISSN: 1618-2642, [retrieved on 20161128], DOI: 10.1007/S00216-016-0100-1 *
GOLDSTEIN ET AL.: "Minimun Formalin Fixation time for consistent estrogen receptor Immunohistochemical staining of invasive breast carcinoma", AMERICAN JOURNAL OF CLINICAL PATHOLOGY, vol. 120, 2003, pages 86 - 92
GOLDSTEIN ET AL.: "Recommendations for improved standardization of Immunohistochemistry", APP. IMMUNOHISTOCHEM MOT MORPHOL., vol. 15, no. 2, June 2007 (2007-06-01), pages 124 - 133, XP055592617
GROELZ DSOBIN LBRANTON PCOMPTON CWYRICH RRAINEN L., EXP MOL PATHOL., vol. 94, 2013, pages 188 - 19
HEWITT ET AL.: "tissue handling and specimen preparation in surgical pathology: issues concerning the recovery of nucleic acids from formalin-fixed, parapin-embedded tissue", ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE, vol. 132, December 2008 (2008-12-01), pages 1929 - 1935
KOSHIBA MOGAWA KHAMAZAKI SSUGIYAMA TOGAWA OKITAJIMA T.: "The effect of formalin fixation on DNA and the extraction of high-molecular-weight DNA from fixed and embedded tissues", PATHOL RES PRACT., vol. 1 89, no. l, February 1993 (1993-02-01), pages 66 - 72
LEWIS ET AL.: "Journal of pathology", vol. 195, 2001, JOHN WILEY & SONS, LTD., article "Unlocking the archive gene expression in paraffinembedded tissue", pages: 66 - 71
MASUDA ET AL.: "nucleic acids Research", vol. 27, 1999, OXFORD UNIVERSITY PRESS, article "Analysis of chemical modification of RNA from formalin-fixed samples and optimization of molecular biology applications for such samples", pages: 4436 - 4443
MEDEIROS ET AL.: "tissue Handling for genome-wide Expression Analysis: A Review of the issues, evidence, and opportunities", ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE, vol. 131, 2007, pages 1805 - 1816
MYTHILY SRINIVASAN ET AL: "Review Effect of Fixatives and Tissue Processing on the Content and Integrity of Nucleic Acids", AM J PATHOL, 1 January 2002 (2002-01-01), pages 1961 - 1971, XP055244114, Retrieved from the Internet <URL:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1850907/pdf/3439.pdf> *
PASKA ET AL.: "effect of formalin, acetone, and RNAIater fixatives on tissue preservation and different size amplicons by real-time PCR from paraffin-embedded tissue", DIAGN MOT PATHOL, vol. 13, no. 4, December 2004 (2004-12-01), pages 234 - 240
SCICCHITANO ET AL.: "preliminary Comparison of Quantity, Quality, and Microarray Performance of RNA Extract from Formalin-fixed, Paraffin-embedded, and uniform frozen tissue samples", JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, vol. 54, 2006, pages 1229 - 1237
SRINIVASAN M. ET AL.: "effect of fixes and tissue processing on the Content and Integrity of nucleic acids", AMERICAN JOURNAL OF PATHOLOGY, vol. 161, 2002, pages 1961 - 1971, XP055244114
STANTA ET AL.: "RNA extracted from paraffin-embedded human tissues is amenable to analysis by PCR amplification", 304 BIOTECHNIQUES, vol. 11, no. 3, 1991, pages 3, XP009094970
VAN MALDEGEM ET AL.: "effects of processing delay, formalin fixation, and immunohistochemistry on RNA Recovery from Formalin-fixed Paraffin-embedded tissue sections", DIAGN MOT PATHOL., vol. 17, no. I, March 2008 (2008-03-01), pages 51 - 58

Also Published As

Publication number Publication date
JP2023507440A (ja) 2023-02-22
WO2021122613A1 (en) 2021-06-24
CA3162334A1 (en) 2021-06-24
US20230017439A1 (en) 2023-01-19
EP4075970A1 (en) 2022-10-26
WO2021122613A9 (en) 2022-06-16
CN114901067A (zh) 2022-08-12

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