IT1318608B1 - Topo transgenico per lo screening e per studi di farmacodinamica efarmacocinetica di ligandi attivi sul recettore degli estrogeni e sui - Google Patents

Topo transgenico per lo screening e per studi di farmacodinamica efarmacocinetica di ligandi attivi sul recettore degli estrogeni e sui

Info

Publication number
IT1318608B1
IT1318608B1 IT2000MI001503A ITMI20001503A IT1318608B1 IT 1318608 B1 IT1318608 B1 IT 1318608B1 IT 2000MI001503 A IT2000MI001503 A IT 2000MI001503A IT MI20001503 A ITMI20001503 A IT MI20001503A IT 1318608 B1 IT1318608 B1 IT 1318608B1
Authority
IT
Italy
Prior art keywords
receptor
extrogen
pharmacokinetics
screening
transgenic mouse
Prior art date
Application number
IT2000MI001503A
Other languages
English (en)
Inventor
Adriana Caterina Maggi
Paolo Ciana
Original Assignee
Univ Degli Studi Milano
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Degli Studi Milano filed Critical Univ Degli Studi Milano
Publication of ITMI20001503A0 publication Critical patent/ITMI20001503A0/it
Priority to IT2000MI001503A priority Critical patent/IT1318608B1/it
Priority to ES01960455T priority patent/ES2321060T3/es
Priority to US10/311,347 priority patent/US7943815B2/en
Priority to PCT/EP2001/007622 priority patent/WO2002001949A2/en
Priority to PT01960455T priority patent/PT1298988E/pt
Priority to DE60136705T priority patent/DE60136705D1/de
Priority to AT01960455T priority patent/ATE415087T1/de
Priority to AU2001281946A priority patent/AU2001281946A1/en
Priority to EP01960455A priority patent/EP1298988B1/en
Priority to EP07119471A priority patent/EP1913813A1/en
Priority to DK01960455T priority patent/DK1298988T3/da
Publication of ITMI20001503A1 publication Critical patent/ITMI20001503A1/it
Application granted granted Critical
Publication of IT1318608B1 publication Critical patent/IT1318608B1/it
Priority to US13/076,807 priority patent/US8809618B2/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/723—Steroid/thyroid hormone superfamily, e.g. GR, EcR, androgen receptor, oestrogen receptor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Environmental Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
IT2000MI001503A 2000-07-04 2000-07-04 Topo transgenico per lo screening e per studi di farmacodinamica efarmacocinetica di ligandi attivi sul recettore degli estrogeni e sui IT1318608B1 (it)

Priority Applications (12)

Application Number Priority Date Filing Date Title
IT2000MI001503A IT1318608B1 (it) 2000-07-04 2000-07-04 Topo transgenico per lo screening e per studi di farmacodinamica efarmacocinetica di ligandi attivi sul recettore degli estrogeni e sui
AT01960455T ATE415087T1 (de) 2000-07-04 2001-07-02 Transgene maus für screening und studium der pharmakokinetik und pharmakodynamik von liganden, welche auf intrazelluläre rezeptoren wirken, sowie methode zur herstellung derselben
EP01960455A EP1298988B1 (en) 2000-07-04 2001-07-02 Transgenic mouse for screening and for studies of the pharmacodynamics and pharmacokinetics of ligands acting on intracellular receptors, and method for the preparation thereof
PCT/EP2001/007622 WO2002001949A2 (en) 2000-07-04 2001-07-02 Transgenic mouse for screening and for studies of the pharmacodynamics and pharmacokinetics of ligands acting on the oestrogen receptor and its intracellular receptors, and method for the preparation thereof
PT01960455T PT1298988E (pt) 2000-07-04 2001-07-02 Ratos transgénicos para a triagem e para estudos da farmacodinâmica e da farmacocinética de ligandos que agem sobre recpetores intracelulares e o seu método de preparação
DE60136705T DE60136705D1 (de) 2000-07-04 2001-07-02 Transgene maus für screening und studium der pharmakokinetik und pharmakodynamik von liganden, welche auf intrazelluläre rezeptoren wirken, sowie methode zur herstellung derselben
ES01960455T ES2321060T3 (es) 2000-07-04 2001-07-02 Raton transgenico para procesos de identificacion y para estudios de la farmacodinamica y farmacocinetica de ligandos que actuan sobre receptores intracelulares y metodo para la preparacion de estos.
AU2001281946A AU2001281946A1 (en) 2000-07-04 2001-07-02 Transgenic mouse for screening and for studies of the pharmacodynamics and pharmacokinetics of ligands acting on the oestrogen receptor and its intracellular receptors, and method for the preparation thereof
US10/311,347 US7943815B2 (en) 2000-07-04 2001-07-02 Transgenic mouse for screening and for studies of the pharmacodynamics and pharmacokinetics of ligands acting on the oestrogen receptor and its intracellular receptors, and method for the preparation thereof
EP07119471A EP1913813A1 (en) 2000-07-04 2001-07-02 Transgenic Mouse for screening and for studies of the pharmacodynamics and pharmacokinetics of ligands acting on intracellular receptors, and method for the preparation thereof
DK01960455T DK1298988T3 (da) 2000-07-04 2001-07-02 Transgen mus til screening og til studier af farmakodynamikken og famakokinetikken af ligander, der virker på intracellulære receptorer, og fremgangsmåde til fremstilling deraf
US13/076,807 US8809618B2 (en) 2000-07-04 2011-03-31 Transgenic mouse for screening and for studies of the pharmacodynamics and pharmacokinetics of ligands acting on the oestrogen receptor and its intracellular receptors, and method for the preparation thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT2000MI001503A IT1318608B1 (it) 2000-07-04 2000-07-04 Topo transgenico per lo screening e per studi di farmacodinamica efarmacocinetica di ligandi attivi sul recettore degli estrogeni e sui

Publications (3)

Publication Number Publication Date
ITMI20001503A0 ITMI20001503A0 (it) 2000-07-04
ITMI20001503A1 ITMI20001503A1 (it) 2002-01-04
IT1318608B1 true IT1318608B1 (it) 2003-08-27

Family

ID=11445401

Family Applications (1)

Application Number Title Priority Date Filing Date
IT2000MI001503A IT1318608B1 (it) 2000-07-04 2000-07-04 Topo transgenico per lo screening e per studi di farmacodinamica efarmacocinetica di ligandi attivi sul recettore degli estrogeni e sui

Country Status (10)

Country Link
US (2) US7943815B2 (it)
EP (2) EP1913813A1 (it)
AT (1) ATE415087T1 (it)
AU (1) AU2001281946A1 (it)
DE (1) DE60136705D1 (it)
DK (1) DK1298988T3 (it)
ES (1) ES2321060T3 (it)
IT (1) IT1318608B1 (it)
PT (1) PT1298988E (it)
WO (1) WO2002001949A2 (it)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0217402D0 (en) * 2002-07-26 2002-09-04 Roslin Inst Edinburgh Multi-reporter gene model for toxicological screening
IT1399356B1 (it) * 2009-11-17 2013-04-16 Top Transgenic Operative Products S R L Animale transgenico per lo screening di composti che modulano la proliferazione cellulare, ed uso in campo farmaceutico.
HU230964B1 (hu) * 2014-11-28 2019-06-28 Magyar Tudományos Akadémia Kísérleti Orvostudományi Kutatóintézet Pajzsmirigyhormon-hatás mérésére szolgáló transzgenikus indikátor egér és rekombináns DNS-konstrukció
JPWO2021054135A1 (it) * 2019-09-19 2021-03-25

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6416998B1 (en) * 1992-09-02 2002-07-09 Baylor College Of Medicine Plasmid encoding a modified steroid hormone
AU732149B2 (en) * 1997-06-17 2001-04-12 United States Of America, Represented By The Secretary, Department Of Health And Human Services, The AIB1, a novel steroid receptor co-activator

Also Published As

Publication number Publication date
AU2001281946A1 (en) 2002-01-14
WO2002001949A2 (en) 2002-01-10
EP1913813A1 (en) 2008-04-23
ATE415087T1 (de) 2008-12-15
ITMI20001503A1 (it) 2002-01-04
DE60136705D1 (de) 2009-01-08
PT1298988E (pt) 2009-03-06
US20030182676A1 (en) 2003-09-25
ITMI20001503A0 (it) 2000-07-04
EP1298988A2 (en) 2003-04-09
ES2321060T3 (es) 2009-06-02
US20110203008A1 (en) 2011-08-18
US7943815B2 (en) 2011-05-17
DK1298988T3 (da) 2009-03-02
WO2002001949A3 (en) 2002-04-18
US8809618B2 (en) 2014-08-19
EP1298988B1 (en) 2008-11-26

Similar Documents

Publication Publication Date Title
Mank et al. A genetically encoded calcium indicator for chronic in vivo two-photon imaging
Kulkarni et al. Voltage imaging: pitfalls and potential
Heidemann et al. Direct observations of the mechanical behaviors of the cytoskeleton in living fibroblasts
Tweedy et al. Self-generated chemotactic gradients—cells steering themselves
Klotzsch et al. A critical survey of methods to detect plasma membrane rafts
ES2179512T3 (es) Pez transgenico con expresion tisular especifica.
Moore et al. Structural determinants of muscle thin filament cooperativity
DE602005013659D1 (de) Elektromagnetische vermessung für kohlenwasserstoff-reservoirs
Vickrey et al. Detection of endogenous dopamine changes in Drosophila melanogaster using fast-scan cyclic voltammetry
Sunnerberg et al. Variations of elastic modulus and cell volume with temperature for cortical neurons
IT1318608B1 (it) Topo transgenico per lo screening e per studi di farmacodinamica efarmacocinetica di ligandi attivi sul recettore degli estrogeni e sui
Larue et al. Modeling melanoblast development
Schneider et al. Nanoscale spatiotemporal diffusion modes measured by simultaneous confocal and stimulated emission depletion nanoscopy imaging
Heine et al. Surface dynamics of voltage-gated ion channels
Kameo et al. Computational framework for analyzing flow-induced strain on osteocyte as modulated by microenvironment
DE69940366D1 (de) Verwendung von porösen perlen für die spitze für eine nano-elektrospray vorrichtung
WO2005088306A3 (en) Biologically-active dna-binding sites and related methods
Kumar et al. Dynamic interaction between actin and nesprin2 maintain the cell nucleus in a prestressed state
WO2000052153A3 (en) Method to identify ligands for orphan receptors
Newell et al. Inter-cycle variation in whole-body vibration exposures of operators driving track-type loader machines
Richhariya et al. dSTIM-and ral/Exocyst-Mediated synaptic release from pupal dopaminergic neurons sustains Drosophila Flight
Shidara et al. Odorant-induced membrane potential depolarization of AIY interneuron in Caenorhabditis elegans
Constantinou et al. The mechanochemistry of integrated motor protein complexes
Heinrich et al. Active mechanical stabilization of the viscoplastic intracellular space of Dictyostelia cells by microtubule–actin crosstalk
Anderson Finding order in a bustling construction zone: quantitative imaging and analysis of cell wall assembly in plants