IT1189104B - Plasmid vector for expression in Bacillus - Google Patents
Plasmid vector for expression in BacillusInfo
- Publication number
- IT1189104B IT1189104B IT20345/86A IT2034586A IT1189104B IT 1189104 B IT1189104 B IT 1189104B IT 20345/86 A IT20345/86 A IT 20345/86A IT 2034586 A IT2034586 A IT 2034586A IT 1189104 B IT1189104 B IT 1189104B
- Authority
- IT
- Italy
- Prior art keywords
- bacillus
- expression
- fragment
- hgh
- plasmid vector
- Prior art date
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
A novel plasmid vector for expression in Bacillus and used for cloning the structural gene which codes for the human growth hormone (hGH) is obtd. by substituting the 750 bp XbaI-ECORI fragment of plasmid pSM143 ATCC 53038 by a synthetic 73 bp fragment having a specified nucleotide sequence. The cloning vector is constructed from pSM143 (ATCC53038) described in IT 19960A/85. The 73 bp fragment was constructed so as to obtain a promoter sequence and a ribosome recognition sequence, both sequences recognised by the RNA polymerase and Bacillus ribosomes and ensuring efficient expression of the structural gene of the hGH placed under control of the sequences.
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IT20345/86A IT1189104B (en) | 1986-05-07 | 1986-05-07 | Plasmid vector for expression in Bacillus |
EP87830142A EP0245218B1 (en) | 1986-05-07 | 1987-04-13 | A plasmid vector for expression in bacillus and used for cloning the structural gene which codes for the human growth hormone and a method of producing the hormone |
ES87830142T ES2061524T3 (en) | 1986-05-07 | 1987-04-13 | A PLASMIDIC VECTOR FOR THE EXPRESSION IN BACILLUS AND EMPLOYEE FOR THE CLONING OF THE STRUCTURAL GENE THAT CODES HUMAN GROWTH HORMONE AND A METHOD FOR HORMONE PRODUCTION. |
DE87830142T DE3788592T2 (en) | 1986-05-07 | 1987-04-13 | Plasmid vector for expression in the bacillus, its application for the cloning of the human growth hormone gene and method for the production of the hormone. |
AT87830142T ATE99359T1 (en) | 1986-05-07 | 1987-04-13 | PLASMID VECTOR FOR EXPRESSION IN BACILLUS, ITS USE FOR CLONING THE HUMAN GROWTH HORMONE GENE AND METHOD OF PRODUCTION OF THE HORMONE. |
JP62109916A JP2540039B2 (en) | 1986-05-07 | 1987-05-07 | Plasmid vector |
US07/837,659 US5334531A (en) | 1986-05-07 | 1992-02-14 | Plasmid vector for expression in bacillus and used for cloning the structural gene which codes for the human growth hormone and a method of producing the hormone |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IT20345/86A IT1189104B (en) | 1986-05-07 | 1986-05-07 | Plasmid vector for expression in Bacillus |
Publications (3)
Publication Number | Publication Date |
---|---|
IT8620345A0 IT8620345A0 (en) | 1986-05-07 |
IT8620345A1 IT8620345A1 (en) | 1987-11-07 |
IT1189104B true IT1189104B (en) | 1988-01-28 |
Family
ID=11165902
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IT20345/86A IT1189104B (en) | 1986-05-07 | 1986-05-07 | Plasmid vector for expression in Bacillus |
Country Status (2)
Country | Link |
---|---|
AT (1) | ATE99359T1 (en) |
IT (1) | IT1189104B (en) |
-
1986
- 1986-05-07 IT IT20345/86A patent/IT1189104B/en active
-
1987
- 1987-04-13 AT AT87830142T patent/ATE99359T1/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
ATE99359T1 (en) | 1994-01-15 |
IT8620345A1 (en) | 1987-11-07 |
IT8620345A0 (en) | 1986-05-07 |
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