IT1189104B - Plasmid vector for expression in Bacillus - Google Patents

Plasmid vector for expression in Bacillus

Info

Publication number
IT1189104B
IT1189104B IT20345/86A IT2034586A IT1189104B IT 1189104 B IT1189104 B IT 1189104B IT 20345/86 A IT20345/86 A IT 20345/86A IT 2034586 A IT2034586 A IT 2034586A IT 1189104 B IT1189104 B IT 1189104B
Authority
IT
Italy
Prior art keywords
bacillus
expression
fragment
hgh
plasmid vector
Prior art date
Application number
IT20345/86A
Other languages
Italian (it)
Other versions
IT8620345A1 (en
IT8620345A0 (en
Inventor
Bue Marina Del
Paola Cosmina
Elisabetta Franchi
Guido Grandi
Original Assignee
Eniricerche Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eniricerche Spa filed Critical Eniricerche Spa
Priority to IT20345/86A priority Critical patent/IT1189104B/en
Publication of IT8620345A0 publication Critical patent/IT8620345A0/en
Priority to EP87830142A priority patent/EP0245218B1/en
Priority to ES87830142T priority patent/ES2061524T3/en
Priority to DE87830142T priority patent/DE3788592T2/en
Priority to AT87830142T priority patent/ATE99359T1/en
Priority to JP62109916A priority patent/JP2540039B2/en
Publication of IT8620345A1 publication Critical patent/IT8620345A1/en
Application granted granted Critical
Publication of IT1189104B publication Critical patent/IT1189104B/en
Priority to US07/837,659 priority patent/US5334531A/en

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  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A novel plasmid vector for expression in Bacillus and used for cloning the structural gene which codes for the human growth hormone (hGH) is obtd. by substituting the 750 bp XbaI-ECORI fragment of plasmid pSM143 ATCC 53038 by a synthetic 73 bp fragment having a specified nucleotide sequence. The cloning vector is constructed from pSM143 (ATCC53038) described in IT 19960A/85. The 73 bp fragment was constructed so as to obtain a promoter sequence and a ribosome recognition sequence, both sequences recognised by the RNA polymerase and Bacillus ribosomes and ensuring efficient expression of the structural gene of the hGH placed under control of the sequences.
IT20345/86A 1986-05-07 1986-05-07 Plasmid vector for expression in Bacillus IT1189104B (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
IT20345/86A IT1189104B (en) 1986-05-07 1986-05-07 Plasmid vector for expression in Bacillus
EP87830142A EP0245218B1 (en) 1986-05-07 1987-04-13 A plasmid vector for expression in bacillus and used for cloning the structural gene which codes for the human growth hormone and a method of producing the hormone
ES87830142T ES2061524T3 (en) 1986-05-07 1987-04-13 A PLASMIDIC VECTOR FOR THE EXPRESSION IN BACILLUS AND EMPLOYEE FOR THE CLONING OF THE STRUCTURAL GENE THAT CODES HUMAN GROWTH HORMONE AND A METHOD FOR HORMONE PRODUCTION.
DE87830142T DE3788592T2 (en) 1986-05-07 1987-04-13 Plasmid vector for expression in the bacillus, its application for the cloning of the human growth hormone gene and method for the production of the hormone.
AT87830142T ATE99359T1 (en) 1986-05-07 1987-04-13 PLASMID VECTOR FOR EXPRESSION IN BACILLUS, ITS USE FOR CLONING THE HUMAN GROWTH HORMONE GENE AND METHOD OF PRODUCTION OF THE HORMONE.
JP62109916A JP2540039B2 (en) 1986-05-07 1987-05-07 Plasmid vector
US07/837,659 US5334531A (en) 1986-05-07 1992-02-14 Plasmid vector for expression in bacillus and used for cloning the structural gene which codes for the human growth hormone and a method of producing the hormone

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT20345/86A IT1189104B (en) 1986-05-07 1986-05-07 Plasmid vector for expression in Bacillus

Publications (3)

Publication Number Publication Date
IT8620345A0 IT8620345A0 (en) 1986-05-07
IT8620345A1 IT8620345A1 (en) 1987-11-07
IT1189104B true IT1189104B (en) 1988-01-28

Family

ID=11165902

Family Applications (1)

Application Number Title Priority Date Filing Date
IT20345/86A IT1189104B (en) 1986-05-07 1986-05-07 Plasmid vector for expression in Bacillus

Country Status (2)

Country Link
AT (1) ATE99359T1 (en)
IT (1) IT1189104B (en)

Also Published As

Publication number Publication date
ATE99359T1 (en) 1994-01-15
IT8620345A1 (en) 1987-11-07
IT8620345A0 (en) 1986-05-07

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