ATE99359T1 - PLASMID VECTOR FOR EXPRESSION IN BACILLUS, ITS USE FOR CLONING THE HUMAN GROWTH HORMONE GENE AND METHOD OF PRODUCTION OF THE HORMONE. - Google Patents

PLASMID VECTOR FOR EXPRESSION IN BACILLUS, ITS USE FOR CLONING THE HUMAN GROWTH HORMONE GENE AND METHOD OF PRODUCTION OF THE HORMONE.

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Publication number
ATE99359T1
ATE99359T1 AT87830142T AT87830142T ATE99359T1 AT E99359 T1 ATE99359 T1 AT E99359T1 AT 87830142 T AT87830142 T AT 87830142T AT 87830142 T AT87830142 T AT 87830142T AT E99359 T1 ATE99359 T1 AT E99359T1
Authority
AT
Austria
Prior art keywords
bacillus
cloning
expression
human growth
plasmid vector
Prior art date
Application number
AT87830142T
Other languages
German (de)
Inventor
Bue Marina Del
Elisabetta Franchi
Paola Cosmina
Guido Grandi
Original Assignee
Eniricerche Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eniricerche Spa filed Critical Eniricerche Spa
Priority claimed from EP87830142A external-priority patent/EP0245218B1/en
Application granted granted Critical
Publication of ATE99359T1 publication Critical patent/ATE99359T1/en

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  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A novel plasmid vector for expression in Bacillus and used for cloning the structural gene which codes for the human growth hormone (hGH) is obtd. by substituting the 750 bp XbaI-ECORI fragment of plasmid pSM143 ATCC 53038 by a synthetic 73 bp fragment having a specified nucleotide sequence. The cloning vector is constructed from pSM143 (ATCC53038) described in IT 19960A/85. The 73 bp fragment was constructed so as to obtain a promoter sequence and a ribosome recognition sequence, both sequences recognised by the RNA polymerase and Bacillus ribosomes and ensuring efficient expression of the structural gene of the hGH placed under control of the sequences.
AT87830142T 1986-05-07 1987-04-13 PLASMID VECTOR FOR EXPRESSION IN BACILLUS, ITS USE FOR CLONING THE HUMAN GROWTH HORMONE GENE AND METHOD OF PRODUCTION OF THE HORMONE. ATE99359T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT20345/86A IT1189104B (en) 1986-05-07 1986-05-07 Plasmid vector for expression in Bacillus
EP87830142A EP0245218B1 (en) 1986-05-07 1987-04-13 A plasmid vector for expression in bacillus and used for cloning the structural gene which codes for the human growth hormone and a method of producing the hormone

Publications (1)

Publication Number Publication Date
ATE99359T1 true ATE99359T1 (en) 1994-01-15

Family

ID=11165902

Family Applications (1)

Application Number Title Priority Date Filing Date
AT87830142T ATE99359T1 (en) 1986-05-07 1987-04-13 PLASMID VECTOR FOR EXPRESSION IN BACILLUS, ITS USE FOR CLONING THE HUMAN GROWTH HORMONE GENE AND METHOD OF PRODUCTION OF THE HORMONE.

Country Status (2)

Country Link
AT (1) ATE99359T1 (en)
IT (1) IT1189104B (en)

Also Published As

Publication number Publication date
IT8620345A1 (en) 1987-11-07
IT1189104B (en) 1988-01-28
IT8620345A0 (en) 1986-05-07

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Legal Events

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UEP Publication of translation of european patent specification
EEIH Change in the person of patent owner
REN Ceased due to non-payment of the annual fee