ES8700321A1 - New expression vectors contg. lambda p1 promoter - Google Patents

New expression vectors contg. lambda p1 promoter

Info

Publication number
ES8700321A1
ES8700321A1 ES546428A ES546428A ES8700321A1 ES 8700321 A1 ES8700321 A1 ES 8700321A1 ES 546428 A ES546428 A ES 546428A ES 546428 A ES546428 A ES 546428A ES 8700321 A1 ES8700321 A1 ES 8700321A1
Authority
ES
Spain
Prior art keywords
dna sequence
contg
vector
host cell
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES546428A
Other languages
Spanish (es)
Other versions
ES546428A0 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Savient Pharmaceuticals Inc
Original Assignee
Savient Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/644,105 external-priority patent/US4742004A/en
Application filed by Savient Pharmaceuticals Inc filed Critical Savient Pharmaceuticals Inc
Publication of ES546428A0 publication Critical patent/ES546428A0/en
Publication of ES8700321A1 publication Critical patent/ES8700321A1/en
Expired legal-status Critical Current

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

(1) Vector that on introduction into a suitable bacterial host cell contg. the thermolabile repressor C (I) renders the host cell capable, when the temp. is raised to a level at which the repressor is inactivated, of effecting expression of a desired gene inserted in the vector to produce polypeptide (I) encoded by the gene is new when it comprises a double-stranded DNA molecule including in 5'- to 3'- order. (a) DNA sequence contg. the promoter and operator P(L)O(L) from lambda bacteriophage; (b) a N utilisation site for binding antiterminator N protein; (c) a first restriction enzyme site permitting replacement of the DNA sequence contg. the ribosomal binding site following after; (d) DNA sequence contg. a ribosomal binding site for making the MRNA of the desired gene capable of binding to ribosomes in the host cell; (e) ATG initiator codon or a DNA sequence converted into the codon on insertion of the desired gene into the vector; and (f) a second restriction enzyme site for inserting the desired gene into the vector in phase with the ATG initiation codon; and (g) a DNA sequence contg. an origin of replication from a bacterial plasmid capable of autonomous replication in the host cell; and for selection, DNA sequence contg. a gene associated with a selectable or identifiable phenotypic trait manifested when the vector is present in the host cell, or DNA sequence contg. fragment C (I) 434, including the gene for the C (I) 434 repressor protein and its associated promoter and operator sequence.
ES546428A 1984-08-27 1985-08-26 New expression vectors contg. lambda p1 promoter Expired ES8700321A1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US64424584A 1984-08-27 1984-08-27
US64467184A 1984-08-27 1984-08-27
US64455184A 1984-08-27 1984-08-27
US06/644,105 US4742004A (en) 1984-08-27 1984-08-27 Method for producing enzymatically active eucaryotic sod in bacteria

Publications (2)

Publication Number Publication Date
ES546428A0 ES546428A0 (en) 1986-10-01
ES8700321A1 true ES8700321A1 (en) 1986-10-01

Family

ID=27505251

Family Applications (3)

Application Number Title Priority Date Filing Date
ES546428A Expired ES8700321A1 (en) 1984-08-27 1985-08-26 New expression vectors contg. lambda p1 promoter
ES552102A Expired ES8704208A1 (en) 1984-08-27 1986-02-17 New expression vectors contg. lambda p1 promoter
ES552104A Expired ES8704209A1 (en) 1984-08-27 1986-02-17 New expression vectors contg. lambda p1 promoter

Family Applications After (2)

Application Number Title Priority Date Filing Date
ES552102A Expired ES8704208A1 (en) 1984-08-27 1986-02-17 New expression vectors contg. lambda p1 promoter
ES552104A Expired ES8704209A1 (en) 1984-08-27 1986-02-17 New expression vectors contg. lambda p1 promoter

Country Status (2)

Country Link
DD (1) DD251787A5 (en)
ES (3) ES8700321A1 (en)

Also Published As

Publication number Publication date
ES546428A0 (en) 1986-10-01
DD251787A5 (en) 1987-11-25
ES8704209A1 (en) 1987-03-16
ES552102A0 (en) 1987-03-16
ES8704208A1 (en) 1987-03-16
ES552104A0 (en) 1987-03-16

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20040701