ES8700321A1 - New expression vectors contg. lambda p1 promoter - Google Patents
New expression vectors contg. lambda p1 promoterInfo
- Publication number
- ES8700321A1 ES8700321A1 ES546428A ES546428A ES8700321A1 ES 8700321 A1 ES8700321 A1 ES 8700321A1 ES 546428 A ES546428 A ES 546428A ES 546428 A ES546428 A ES 546428A ES 8700321 A1 ES8700321 A1 ES 8700321A1
- Authority
- ES
- Spain
- Prior art keywords
- dna sequence
- contg
- vector
- host cell
- gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
(1) Vector that on introduction into a suitable bacterial host cell contg. the thermolabile repressor C (I) renders the host cell capable, when the temp. is raised to a level at which the repressor is inactivated, of effecting expression of a desired gene inserted in the vector to produce polypeptide (I) encoded by the gene is new when it comprises a double-stranded DNA molecule including in 5'- to 3'- order. (a) DNA sequence contg. the promoter and operator P(L)O(L) from lambda bacteriophage; (b) a N utilisation site for binding antiterminator N protein; (c) a first restriction enzyme site permitting replacement of the DNA sequence contg. the ribosomal binding site following after; (d) DNA sequence contg. a ribosomal binding site for making the MRNA of the desired gene capable of binding to ribosomes in the host cell; (e) ATG initiator codon or a DNA sequence converted into the codon on insertion of the desired gene into the vector; and (f) a second restriction enzyme site for inserting the desired gene into the vector in phase with the ATG initiation codon; and (g) a DNA sequence contg. an origin of replication from a bacterial plasmid capable of autonomous replication in the host cell; and for selection, DNA sequence contg. a gene associated with a selectable or identifiable phenotypic trait manifested when the vector is present in the host cell, or DNA sequence contg. fragment C (I) 434, including the gene for the C (I) 434 repressor protein and its associated promoter and operator sequence.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US64424584A | 1984-08-27 | 1984-08-27 | |
US64467184A | 1984-08-27 | 1984-08-27 | |
US64455184A | 1984-08-27 | 1984-08-27 | |
US06/644,105 US4742004A (en) | 1984-08-27 | 1984-08-27 | Method for producing enzymatically active eucaryotic sod in bacteria |
Publications (2)
Publication Number | Publication Date |
---|---|
ES546428A0 ES546428A0 (en) | 1986-10-01 |
ES8700321A1 true ES8700321A1 (en) | 1986-10-01 |
Family
ID=27505251
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES546428A Expired ES8700321A1 (en) | 1984-08-27 | 1985-08-26 | New expression vectors contg. lambda p1 promoter |
ES552102A Expired ES8704208A1 (en) | 1984-08-27 | 1986-02-17 | New expression vectors contg. lambda p1 promoter |
ES552104A Expired ES8704209A1 (en) | 1984-08-27 | 1986-02-17 | New expression vectors contg. lambda p1 promoter |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES552102A Expired ES8704208A1 (en) | 1984-08-27 | 1986-02-17 | New expression vectors contg. lambda p1 promoter |
ES552104A Expired ES8704209A1 (en) | 1984-08-27 | 1986-02-17 | New expression vectors contg. lambda p1 promoter |
Country Status (2)
Country | Link |
---|---|
DD (1) | DD251787A5 (en) |
ES (3) | ES8700321A1 (en) |
-
1985
- 1985-08-26 ES ES546428A patent/ES8700321A1/en not_active Expired
- 1985-08-27 DD DD28002185A patent/DD251787A5/en unknown
-
1986
- 1986-02-17 ES ES552102A patent/ES8704208A1/en not_active Expired
- 1986-02-17 ES ES552104A patent/ES8704209A1/en not_active Expired
Also Published As
Publication number | Publication date |
---|---|
ES546428A0 (en) | 1986-10-01 |
DD251787A5 (en) | 1987-11-25 |
ES8704209A1 (en) | 1987-03-16 |
ES552102A0 (en) | 1987-03-16 |
ES8704208A1 (en) | 1987-03-16 |
ES552104A0 (en) | 1987-03-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 20040701 |