IN2015DN03186A - - Google Patents
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- Publication number
- IN2015DN03186A IN2015DN03186A IN3186DEN2015A IN2015DN03186A IN 2015DN03186 A IN2015DN03186 A IN 2015DN03186A IN 3186DEN2015 A IN3186DEN2015 A IN 3186DEN2015A IN 2015DN03186 A IN2015DN03186 A IN 2015DN03186A
- Authority
- IN
- India
- Prior art keywords
- cells
- fetal
- endocervical sample
- disassociated
- isolating
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2503/00—Use of cells in diagnostics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/70503—Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3
- G01N2333/70539—MHC-molecules, e.g. HLA-molecules
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/36—Gynecology or obstetrics
- G01N2800/368—Pregnancy complicated by disease or abnormalities of pregnancy, e.g. preeclampsia, preterm labour
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Developmental Biology & Embryology (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- Pathology (AREA)
- Biophysics (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pregnancy & Childbirth (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A method of retrieving fetal cells from an endocervical sample by removing the mucus from the endocervical sample by disassociating fetal cells and maternal cells in the endocervical sample; and isolating disassociated fetal cells from other cells in the endocervical sample. Also provided is a method of retrieving fetal cells from an endocervical sample, by obtaining a mixture of disassociated cells prepared by the above method, treating the cells with a fetal- specific antibody , identifying cells that have bound to the fetal- specific antibody , and isolating the identified cells. The disassociated cell prepared by the above method can be analyzed and used for a variety of purposes including, but not limited to, the identification of fetal cells among cervical cells , determination of fetal cell density to predict high risk pregnancy , genetic analysis of fetal cells ,and determination of growth factor or other biomarker expression to predict obstetrical disorders.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261715854P | 2012-10-19 | 2012-10-19 | |
PCT/US2013/065570 WO2014062995A1 (en) | 2012-10-19 | 2013-10-18 | Identification and analysis of fetal trophoblast cells in cervical mucus for prenatal diagnosis |
Publications (1)
Publication Number | Publication Date |
---|---|
IN2015DN03186A true IN2015DN03186A (en) | 2015-10-02 |
Family
ID=50488767
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IN3186DEN2015 IN2015DN03186A (en) | 2012-10-19 | 2013-10-18 |
Country Status (11)
Country | Link |
---|---|
US (1) | US10344315B2 (en) |
EP (1) | EP2909315B1 (en) |
JP (1) | JP6421123B2 (en) |
AU (1) | AU2013331171B2 (en) |
CA (1) | CA2888746C (en) |
DK (1) | DK2909315T3 (en) |
ES (1) | ES2729182T3 (en) |
HK (1) | HK1214294A1 (en) |
IN (1) | IN2015DN03186A (en) |
TR (1) | TR201908019T4 (en) |
WO (1) | WO2014062995A1 (en) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IN2015DN03186A (en) | 2012-10-19 | 2015-10-02 | Univ Wayne State | |
CN107367618B (en) * | 2014-10-10 | 2020-02-14 | 韦恩州立大学 | Methods and compositions related to fetal extravillous trophoblast cell assays |
JP7026098B2 (en) | 2016-04-06 | 2022-02-25 | ウェイン ステート ユニバーシティ | Isolation and analysis of fetal DNA from extravillous trophoblast cells collected from the cervix |
WO2017180909A1 (en) | 2016-04-13 | 2017-10-19 | Nextgen Jane, Inc. | Sample collection and preservation devices, systems and methods |
WO2019021065A2 (en) * | 2017-02-13 | 2019-01-31 | Trophodiagnostics, Llc | New system and method for collecting, enriching, and isolating trophoblast cells from endocervical canal |
US11230729B2 (en) | 2018-04-20 | 2022-01-25 | Inanna Diagnostics, Inc | Methods and devices for obtaining cellular and DNA material from human female reproductive system |
TWI668423B (en) * | 2018-10-02 | 2019-08-11 | 吳宏偉 | Cell sorting method and system |
CN111122857A (en) * | 2018-10-31 | 2020-05-08 | 苏州浚惠生物科技有限公司 | Marker of fetal trophoblast cells, identification method, detection kit and application |
CN111304153A (en) * | 2019-12-17 | 2020-06-19 | 广东凯普生物科技股份有限公司 | Method for separating trophoblast cells |
WO2021167098A1 (en) * | 2020-02-21 | 2021-08-26 | 国立大学法人東海国立大学機構 | Quantitative assessment index for fetal growth restriction |
CN112980779B (en) * | 2021-05-20 | 2021-08-24 | 广州凯普医药科技有限公司 | Method for separating placenta trophoblast cells from cervical exfoliated cells of pregnant women |
Family Cites Families (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5629147A (en) | 1992-07-17 | 1997-05-13 | Aprogenex, Inc. | Enriching and identifying fetal cells in maternal blood for in situ hybridization |
EP0587951B1 (en) | 1992-09-18 | 1997-12-17 | AMERSHAM INTERNATIONAL plc | Cell nuclei capture method and device used therefor |
BR9907852A (en) * | 1998-02-12 | 2000-10-24 | Immunivest Corp | Processes to detect and enumerate rare and cancerous cells in a mixed cell population, to diagnose early stage cancer in a test patient, to determine the likelihood of cancer recurrence in a previously treated human patient from cancer, to distinguish a carcinoma confined to the organ of a carcinoma with metastatic properties, to monitor the remission situation in a human cancer patient undergoing cancer therapy treatment and to increase amounts of circulating epithelial cells in a blood sample, coated magnetic particle, composition, sets test to assess a patient sample for the presence of rare circulating cells, for the presence of circulating tumor cells, for the presence of circulating breast cancer cells, for the presence of circulating prostate cancer cells, for the presence of circulating colon cancer cells , regarding the presence of circulating bladder cancer cells and to monitor a patient for cancer recurrence, and, peripheral blood fraction enriched for circulating neoplastic cells |
AUPR749901A0 (en) | 2001-09-06 | 2001-09-27 | Monash University | Method of identifying chromosomal abnormalities and prenatal diagnosis |
US20040197832A1 (en) | 2003-04-03 | 2004-10-07 | Mor Research Applications Ltd. | Non-invasive prenatal genetic diagnosis using transcervical cells |
US20050181429A1 (en) * | 2003-04-03 | 2005-08-18 | Monaliza Medical Ltd. | Non-invasive prenatal genetic diagnosis using transcervical cells |
CA2575675A1 (en) | 2004-07-30 | 2006-03-09 | Adeza Biomedical Corporation | Oncofetal fibronectin as a marker for disease and other conditions and methods for detection of oncofetal fibronectin |
US20070224597A1 (en) | 2006-03-23 | 2007-09-27 | Biocept, Inc. | Isolating fetal trophoblasts |
US20090286271A1 (en) | 2006-05-31 | 2009-11-19 | Karumanchi Ananth S | Methods of Diagnosing and Treating Complications of Pregnancy |
CA2713545A1 (en) | 2008-01-30 | 2009-08-06 | Novartis Ag | Two stage enrichment of cell-free fetal dna in maternal plasma |
EP2250497B1 (en) * | 2008-02-18 | 2014-09-10 | Genetic Technologies Limited | Cell processing and/or enrichment methods |
SG175282A1 (en) | 2009-04-21 | 2011-11-28 | Genetic Technologies Ltd | Methods for obtaining fetal genetic material |
IN2015DN03186A (en) | 2012-10-19 | 2015-10-02 | Univ Wayne State |
-
2013
- 2013-10-18 IN IN3186DEN2015 patent/IN2015DN03186A/en unknown
- 2013-10-18 DK DK13847056.2T patent/DK2909315T3/en active
- 2013-10-18 ES ES13847056T patent/ES2729182T3/en active Active
- 2013-10-18 TR TR2019/08019T patent/TR201908019T4/en unknown
- 2013-10-18 JP JP2015538043A patent/JP6421123B2/en active Active
- 2013-10-18 US US14/436,270 patent/US10344315B2/en active Active
- 2013-10-18 WO PCT/US2013/065570 patent/WO2014062995A1/en active Application Filing
- 2013-10-18 AU AU2013331171A patent/AU2013331171B2/en active Active
- 2013-10-18 CA CA2888746A patent/CA2888746C/en active Active
- 2013-10-18 EP EP13847056.2A patent/EP2909315B1/en active Active
-
2016
- 2016-02-23 HK HK16102021.1A patent/HK1214294A1/en unknown
Also Published As
Publication number | Publication date |
---|---|
JP2015533504A (en) | 2015-11-26 |
CA2888746A1 (en) | 2014-04-24 |
US20150267240A1 (en) | 2015-09-24 |
JP6421123B2 (en) | 2018-11-07 |
AU2013331171B2 (en) | 2019-05-30 |
WO2014062995A1 (en) | 2014-04-24 |
HK1214294A1 (en) | 2016-07-22 |
EP2909315A4 (en) | 2016-04-20 |
DK2909315T3 (en) | 2019-06-03 |
AU2013331171A1 (en) | 2015-05-28 |
ES2729182T3 (en) | 2019-10-30 |
EP2909315A1 (en) | 2015-08-26 |
EP2909315B1 (en) | 2019-05-01 |
TR201908019T4 (en) | 2019-06-21 |
US10344315B2 (en) | 2019-07-09 |
CA2888746C (en) | 2022-07-26 |
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