IN2015DN03186A - - Google Patents

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Publication number
IN2015DN03186A
IN2015DN03186A IN3186DEN2015A IN2015DN03186A IN 2015DN03186 A IN2015DN03186 A IN 2015DN03186A IN 3186DEN2015 A IN3186DEN2015 A IN 3186DEN2015A IN 2015DN03186 A IN2015DN03186 A IN 2015DN03186A
Authority
IN
India
Prior art keywords
cells
fetal
endocervical sample
disassociated
isolating
Prior art date
Application number
Inventor
D Randall Armant
Michael P Diamond
Original Assignee
Univ Wayne State
Michael P Diamond
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Wayne State, Michael P Diamond filed Critical Univ Wayne State
Publication of IN2015DN03186A publication Critical patent/IN2015DN03186A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70503—Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3
    • G01N2333/70539—MHC-molecules, e.g. HLA-molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/36—Gynecology or obstetrics
    • G01N2800/368—Pregnancy complicated by disease or abnormalities of pregnancy, e.g. preeclampsia, preterm labour

Abstract

A method of retrieving fetal cells from an endocervical sample by removing the mucus from the endocervical sample by disassociating fetal cells and maternal cells in the endocervical sample; and isolating disassociated fetal cells from other cells in the endocervical sample. Also provided is a method of retrieving fetal cells from an endocervical sample, by obtaining a mixture of disassociated cells prepared by the above method, treating the cells with a fetal- specific antibody , identifying cells that have bound to the fetal- specific antibody , and isolating the identified cells. The disassociated cell prepared by the above method can be analyzed and used for a variety of purposes including, but not limited to, the identification of fetal cells among cervical cells , determination of fetal cell density to predict high risk pregnancy , genetic analysis of fetal cells ,and determination of growth factor or other biomarker expression to predict obstetrical disorders.
IN3186DEN2015 2012-10-19 2013-10-18 IN2015DN03186A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261715854P 2012-10-19 2012-10-19
PCT/US2013/065570 WO2014062995A1 (en) 2012-10-19 2013-10-18 Identification and analysis of fetal trophoblast cells in cervical mucus for prenatal diagnosis

Publications (1)

Publication Number Publication Date
IN2015DN03186A true IN2015DN03186A (en) 2015-10-02

Family

ID=50488767

Family Applications (1)

Application Number Title Priority Date Filing Date
IN3186DEN2015 IN2015DN03186A (en) 2012-10-19 2013-10-18

Country Status (11)

Country Link
US (1) US10344315B2 (en)
EP (1) EP2909315B1 (en)
JP (1) JP6421123B2 (en)
AU (1) AU2013331171B2 (en)
CA (1) CA2888746C (en)
DK (1) DK2909315T3 (en)
ES (1) ES2729182T3 (en)
HK (1) HK1214294A1 (en)
IN (1) IN2015DN03186A (en)
TR (1) TR201908019T4 (en)
WO (1) WO2014062995A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2909315T3 (en) 2012-10-19 2019-06-03 Univ Wayne State IDENTIFICATION AND ANALYSIS OF FEED TROPHY LOAD CELLS IN CERVIX SLIM FOR PRINATIC DIAGNOSIS
CA2963968C (en) * 2014-10-10 2024-01-09 Wayne State University Methods and compositions relating to assays of fetal extravillous trophoblast cells
AU2017246480B2 (en) * 2016-04-06 2023-06-01 Wayne State University Isolation and analysis of fetal DNA from extravillous trophoblast cells retrieved from the endocervical canal
WO2017180909A1 (en) 2016-04-13 2017-10-19 Nextgen Jane, Inc. Sample collection and preservation devices, systems and methods
WO2019021065A2 (en) * 2017-02-13 2019-01-31 Trophodiagnostics, Llc New system and method for collecting, enriching, and isolating trophoblast cells from endocervical canal
US11230729B2 (en) 2018-04-20 2022-01-25 Inanna Diagnostics, Inc Methods and devices for obtaining cellular and DNA material from human female reproductive system
TWI668423B (en) * 2018-10-02 2019-08-11 吳宏偉 Cell sorting method and system
CN111122857A (en) * 2018-10-31 2020-05-08 苏州浚惠生物科技有限公司 Marker of fetal trophoblast cells, identification method, detection kit and application
CN111304153A (en) * 2019-12-17 2020-06-19 广东凯普生物科技股份有限公司 Method for separating trophoblast cells
WO2021167098A1 (en) * 2020-02-21 2021-08-26 国立大学法人東海国立大学機構 Quantitative assessment index for fetal growth restriction
CN112980779B (en) * 2021-05-20 2021-08-24 广州凯普医药科技有限公司 Method for separating placenta trophoblast cells from cervical exfoliated cells of pregnant women

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US5629147A (en) 1992-07-17 1997-05-13 Aprogenex, Inc. Enriching and identifying fetal cells in maternal blood for in situ hybridization
DE69223617T2 (en) 1992-09-18 1998-04-09 Amersham Int Plc Process for collecting cell nuclei and device used therefor
EP1062515B1 (en) * 1998-02-12 2009-11-25 Immunivest Corporation Methods and reagents for the rapid and efficient isolation of circulating cancer cells
AUPR749901A0 (en) 2001-09-06 2001-09-27 Monash University Method of identifying chromosomal abnormalities and prenatal diagnosis
US20050181429A1 (en) 2003-04-03 2005-08-18 Monaliza Medical Ltd. Non-invasive prenatal genetic diagnosis using transcervical cells
US20040197832A1 (en) * 2003-04-03 2004-10-07 Mor Research Applications Ltd. Non-invasive prenatal genetic diagnosis using transcervical cells
CA2575675A1 (en) 2004-07-30 2006-03-09 Adeza Biomedical Corporation Oncofetal fibronectin as a marker for disease and other conditions and methods for detection of oncofetal fibronectin
US20070224597A1 (en) * 2006-03-23 2007-09-27 Biocept, Inc. Isolating fetal trophoblasts
US20090286271A1 (en) 2006-05-31 2009-11-19 Karumanchi Ananth S Methods of Diagnosing and Treating Complications of Pregnancy
JP2011510660A (en) 2008-01-30 2011-04-07 ノバルティス アーゲー Two-step concentration method of cell-free fetal DNA in maternal plasma
CA2752838A1 (en) * 2008-02-18 2009-08-27 Genetic Technologies Limited Cell processing and/or enrichment methods
EP2421955A4 (en) * 2009-04-21 2012-10-10 Genetic Technologies Ltd Methods for obtaining fetal genetic material
DK2909315T3 (en) 2012-10-19 2019-06-03 Univ Wayne State IDENTIFICATION AND ANALYSIS OF FEED TROPHY LOAD CELLS IN CERVIX SLIM FOR PRINATIC DIAGNOSIS

Also Published As

Publication number Publication date
EP2909315B1 (en) 2019-05-01
CA2888746C (en) 2022-07-26
EP2909315A1 (en) 2015-08-26
ES2729182T3 (en) 2019-10-30
EP2909315A4 (en) 2016-04-20
US20150267240A1 (en) 2015-09-24
WO2014062995A1 (en) 2014-04-24
US10344315B2 (en) 2019-07-09
JP2015533504A (en) 2015-11-26
HK1214294A1 (en) 2016-07-22
AU2013331171A1 (en) 2015-05-28
TR201908019T4 (en) 2019-06-21
JP6421123B2 (en) 2018-11-07
CA2888746A1 (en) 2014-04-24
DK2909315T3 (en) 2019-06-03
AU2013331171B2 (en) 2019-05-30

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