IN2014DN10264A - - Google Patents

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Publication number
IN2014DN10264A
IN2014DN10264A IN10264DEN2014A IN2014DN10264A IN 2014DN10264 A IN2014DN10264 A IN 2014DN10264A IN 10264DEN2014 A IN10264DEN2014 A IN 10264DEN2014A IN 2014DN10264 A IN2014DN10264 A IN 2014DN10264A
Authority
IN
India
Prior art keywords
fast bacteria
test sample
acid fast
container
acid
Prior art date
Application number
Inventor
Jones M Hyman
Parampal Deol
Elizabeth Miller
Victoria Girard
Amber Gates
Sandrine Mailler
Maud Arsac
John Walsh
Original Assignee
Bio Merieux Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Merieux Inc filed Critical Bio Merieux Inc
Publication of IN2014DN10264A publication Critical patent/IN2014DN10264A/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/5695—Mycobacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/35—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Mycobacteriaceae (F)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/355—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Nocardia (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2560/00—Chemical aspects of mass spectrometric analysis of biological material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Optics & Photonics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

The present invention is directed to a method for inactivation and/or extraction of acid fast bacteria (e.g. mycobacteria or nocardia) the method comprising the following sequential steps: (a) acquiring a test sample known to contain or that may contain acid fast bacteria and suspending the test sample in a container containing ethanol and beads; (b) bead beating and/or vortexing the container to break up clumps and/or disrupt acid fast bacteria cells in the container; and (c) subsequently incubating the suspension for at least about 3 minutes at room temperature to inactivate any acid fast bacteria contained in the test sample. In accordance with the present invention the test sample can subsequently be pelleted by centrifugation resuspended with formic acid acetonitrile added and subjected to mass spectrometry for characterization and/or identification of the acid fast bacteria.
IN10264DEN2014 2012-05-17 2013-05-15 IN2014DN10264A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261648420P 2012-05-17 2012-05-17
US13/828,119 US8735091B2 (en) 2012-05-17 2013-03-14 Methods for inactivation and extraction of acid-fast bacteria for characterization and/or identification using mass spectrometry
PCT/US2013/041159 WO2013173466A1 (en) 2012-05-17 2013-05-15 Methods for inactivation and extraction of acid-fast bacteria for characterization and/or identification using mass spectrometry

Publications (1)

Publication Number Publication Date
IN2014DN10264A true IN2014DN10264A (en) 2015-08-07

Family

ID=49581609

Family Applications (1)

Application Number Title Priority Date Filing Date
IN10264DEN2014 IN2014DN10264A (en) 2012-05-17 2013-05-15

Country Status (9)

Country Link
US (1) US8735091B2 (en)
EP (1) EP2850204B1 (en)
JP (1) JP6270824B2 (en)
CN (2) CN111504758A (en)
AU (1) AU2013262852B2 (en)
CA (1) CA2872494C (en)
ES (1) ES2690589T3 (en)
IN (1) IN2014DN10264A (en)
WO (1) WO2013173466A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2951548B1 (en) * 2009-10-15 2011-11-11 Biomerieux Sa METHOD FOR CHARACTERIZING AT LEAST ONE MICROORGANISM BY MASS SPECTROMETRY
HK1243454A1 (en) * 2014-09-17 2018-07-13 Hologic, Inc. Method of partial lysis and assay
US10626437B2 (en) 2015-08-24 2020-04-21 Biomerieux, Inc. Methods for inactivation and extraction of acid-fast bacteria from liquid media for characterization and/or identification using mass spectrometry
WO2021055809A1 (en) * 2019-09-18 2021-03-25 Research Development Foundation Methods and probiotic compositions for the treatment of metabolic diseases and disorders
DE102019126201A1 (en) * 2019-09-27 2021-04-01 Bruker Daltonik Gmbh Mass spectrometric sample preparation for matrix-assisted ionization (e.g. MALDI)
KR102534015B1 (en) * 2021-01-27 2023-05-19 (재)씨젠의료재단 A Method for Isolating Intact protein derived from gram-negative bacteria
CN117092199B (en) * 2023-10-16 2024-01-26 成都泰莱医学检验实验室有限公司 Method for time-of-flight mass spectrometry detection using tungsten carbide nanomaterial matrix

Family Cites Families (19)

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Publication number Priority date Publication date Assignee Title
US3745090A (en) * 1970-08-04 1973-07-10 Nasa Method of detecting and counting bacteria in body fluids
US5512440A (en) * 1991-12-18 1996-04-30 Becton Dickinson And Company Process for lysing Mycobacteria
US5942425A (en) * 1996-03-12 1999-08-24 Walters; Adriann H. Method to access nucleic acids from cells
US6177266B1 (en) 1996-04-29 2001-01-23 The United States Of America As Represented By The Secretary Of The Army Rapid identification of bacteria by mass spectrometry
US6723564B2 (en) * 1998-05-07 2004-04-20 Sequenom, Inc. IR MALDI mass spectrometry of nucleic acids using liquid matrices
US5948610A (en) 1998-06-03 1999-09-07 University Of Maryland At Baltimore County Use of matrices comprising liquids and light absorbing particles for analysis of microorganisms by laser desorption mass spectrometry
US7020559B1 (en) 1998-11-17 2006-03-28 University Of Maryland Methods for identifying and classifying organisms by mass spectrometry and database searching
US6833249B2 (en) * 2002-03-22 2004-12-21 Council Of Scientific And Industrial Research Quick and sensitive method of quantifying mycolic acid to develop anti-microbial agents and a diagnostic kit thereof
AU2003237677A1 (en) * 2002-05-23 2003-12-12 Tenaxis Gmbh Screening for compounds having an effect on cells using microtiter plates
KR20050052467A (en) 2002-08-12 2005-06-02 다이나박스 테크놀로지 코퍼레이션 Immunomodulatory compositions, methods of making, and methods of use thereof
WO2004044555A2 (en) 2002-11-12 2004-05-27 Becton, Dickinson And Company Diagnosis of sepsis or sirs using biomarker profiles
EP1897957B1 (en) * 2003-07-29 2010-03-31 All India Institute of Medical Sciences A method for diagnosis of tuberculosis by smear microscopy, culture and polymerase chain reaction using processed clinical samples and kit thereof
DE102006021493B4 (en) 2006-05-09 2010-04-01 Bruker Daltonik Gmbh Mass spectrometric measurement of microbial resistance
DE102007058516B4 (en) 2007-11-23 2017-08-10 Bruker Daltonik Gmbh Identification of pathogens in body fluids
CN103123353B (en) * 2007-12-28 2014-11-12 株式会社比尔生命 Immunodetection assay for mycobacterium tuberculosis complex
EP2291400A4 (en) * 2008-05-26 2011-07-20 Tyrian Diagnostics Ltd Method of diagnosis of infection by mycobacteria and reagents therefor
CA2741019C (en) * 2008-10-31 2018-08-28 Biomerieux, Inc. Methods for separation, characterization and/or identification of microorganisms using mass spectrometry
US20120165246A1 (en) * 2009-02-26 2012-06-28 Tyrian Diagnostics Limited Method of Diagnosis of Infection by Mycobacteria and Reagents Therefor
US10634588B2 (en) * 2010-09-24 2020-04-28 Qiagen Gmbh Reagent for the disruption of cell material having a completely integrated internal standard

Also Published As

Publication number Publication date
EP2850204A1 (en) 2015-03-25
CN111504758A (en) 2020-08-07
AU2013262852B2 (en) 2018-10-04
JP6270824B2 (en) 2018-01-31
US8735091B2 (en) 2014-05-27
CN104284985A (en) 2015-01-14
CA2872494A1 (en) 2013-11-21
ES2690589T3 (en) 2018-11-21
WO2013173466A1 (en) 2013-11-21
JP2015517315A (en) 2015-06-22
EP2850204B1 (en) 2018-07-11
US20130309714A1 (en) 2013-11-21
AU2013262852A1 (en) 2014-11-27
CA2872494C (en) 2022-10-04

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