IN2012DN06442A - - Google Patents

Info

Publication number
IN2012DN06442A
IN2012DN06442A IN6442DEN2012A IN2012DN06442A IN 2012DN06442 A IN2012DN06442 A IN 2012DN06442A IN 6442DEN2012 A IN6442DEN2012 A IN 6442DEN2012A IN 2012DN06442 A IN2012DN06442 A IN 2012DN06442A
Authority
IN
India
Prior art keywords
transformation
threonine
attenuated
reducing
modified strains
Prior art date
Application number
Inventor
Cédric Boisart
Corre Gwénaëlle Bestel
Guillaume Barbier
Rainer Figge
Original Assignee
Metabolic Explorer Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metabolic Explorer Sa filed Critical Metabolic Explorer Sa
Publication of IN2012DN06442A publication Critical patent/IN2012DN06442A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1014—Hydroxymethyl-, formyl-transferases (2.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/12—Methionine; Cysteine; Cystine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/02—Hydroxymethyl-, formyl- and related transferases (2.1.2)
    • C12Y201/02001—Glycine hydroxymethyltransferase (2.1.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y403/00—Carbon-nitrogen lyases (4.3)
    • C12Y403/01—Ammonia-lyases (4.3.1)
    • C12Y403/01019—Threonine ammonia-lyase (4.3.1.19)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cephalosporin Compounds (AREA)

Abstract

The present invention relates to a method for the production of methionine using modified strains with attenuated transformation of threonine. This can be achieved by reducing threonine transformation intoglycine and/or by reducing its transformation to a ketobutyrate. The invention also concerns the modified strains with attenuated transformation of threonine.
IN6442DEN2012 2009-12-30 2010-12-29 IN2012DN06442A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US29097509P 2009-12-30 2009-12-30
EP09306349 2009-12-30
PCT/EP2010/070858 WO2011080301A2 (en) 2009-12-30 2010-12-29 Strains and method for the production of methionine

Publications (1)

Publication Number Publication Date
IN2012DN06442A true IN2012DN06442A (en) 2015-10-09

Family

ID=42562397

Family Applications (1)

Application Number Title Priority Date Filing Date
IN6442DEN2012 IN2012DN06442A (en) 2009-12-30 2010-12-29

Country Status (11)

Country Link
US (1) US9382561B2 (en)
EP (1) EP2519637A2 (en)
JP (1) JP5922036B2 (en)
KR (1) KR101915819B1 (en)
CN (1) CN102782137B (en)
AR (1) AR079821A1 (en)
BR (1) BR112012016908B1 (en)
IN (1) IN2012DN06442A (en)
MX (1) MX2012007717A (en)
MY (1) MY172018A (en)
WO (1) WO2011080301A2 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2519637A2 (en) 2009-12-30 2012-11-07 Metabolic Explorer Strains and method for the production of methionine
CN103981206B (en) * 2014-06-06 2016-03-02 中国科学院天津工业生物技术研究所 A kind of method of screening high yield L-threonine-producing strain
CA2959563A1 (en) 2014-09-01 2016-03-10 Metabolic Explorer Method and microorganism for methionine production by fermentation with improved methionine efflux
CN104313071B (en) * 2014-10-17 2018-04-13 湖南宝利士生物技术有限公司 The biological synthesis method of high-purity L alpha amino acids
CN104372035B (en) * 2014-10-17 2017-10-31 湖南宝利士生物技术有限公司 The method for synthesizing high-purity 2 ketonic acid salt
DK3250701T3 (en) * 2015-01-27 2021-02-08 Cysbio Aps Genetically modified microorganisms with improved tolerance to L-serine
US20180223319A1 (en) 2015-08-07 2018-08-09 Evonik Degussa Gmbh Protein thiocarboxylate-dependent l-methionine production by fermentation
CN108291243A (en) 2015-11-27 2018-07-17 赢创德固赛有限公司 The method for producing l-methionine
EP3296404A1 (en) 2016-09-15 2018-03-21 Evonik Degussa GmbH Modified microorganism for production of methionine
WO2018213796A1 (en) * 2017-05-19 2018-11-22 Zymergen Inc. Genomic engineering of biosynthetic pathways leading to increased nadph
CN109402098B (en) * 2018-11-06 2021-09-17 王喆明 Threonine aldolase, mutant and application of mutant in preparation of substituted phenylserine derivative
US20200393381A1 (en) * 2019-06-12 2020-12-17 Henkel IP & Holding GmbH Method And System For Determining The Presence Of An Odorous Material On A Fabric
CN112779200B (en) * 2021-01-12 2022-07-08 浙江工业大学 Genetically engineered bacterium for high yield of L-methionine and construction and application thereof
WO2024096123A1 (en) * 2022-11-04 2024-05-10 株式会社バイオパレット Genetically modified microorganism and method for producing same
WO2024149616A1 (en) 2023-01-09 2024-07-18 Evonik Operations Gmbh Fermentative production guanidinoacetic acid (gaa) from serine using a microorganism having an enhanced l-serine hydroxymethyltransferase activity
WO2024149617A1 (en) 2023-01-09 2024-07-18 Evonik Operations Gmbh Fermentative production guanidinoacetic acid (gaa) from serine by attenuating l serine ammonia lyase activity in microorganisms

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000157267A (en) 1998-11-24 2000-06-13 Ajinomoto Co Inc VARIATION TYPE metJ GENE AND PRODUCTION OF L-METHIONINE
JPWO2002097086A1 (en) 2001-05-29 2005-04-07 協和醗酵工業株式会社 Microorganisms useful for industrial production
DE10305774A1 (en) * 2003-02-06 2004-08-26 Consortium für elektrochemische Industrie GmbH Process for the fermentative production of L-methionine
MXPA05008857A (en) 2003-02-18 2006-03-09 Metabolic Explorer Sa Method for the production of evolved microorganisms which permit the generation or modification of metabolic pathways.
DE10311399A1 (en) * 2003-03-13 2004-09-23 Forschungszentrum Jülich GmbH New nucleic acid encoding inactive L-serine dehydratase, useful for improving bacterial synthesis of L-serine, is a deleted or mutated form of the wild-type sequence
WO2005111202A1 (en) 2004-05-12 2005-11-24 Metabolic Explorer Recombinant enzyme with altered feedback sensitivity
NO331033B1 (en) * 2005-07-07 2011-09-19 I P Huse As Device for transporting chain.
JP2009504172A (en) 2005-08-18 2009-02-05 ビーエーエスエフ ソシエタス・ヨーロピア Microorganisms with improved methionine synthesis efficiency
WO2007077041A1 (en) * 2006-01-04 2007-07-12 Metabolic Explorer Process for the preparation of methionine and its precursors homoserine or succinylhomoserine employing a microorganism with enhanced sulfate permease expression
KR100905381B1 (en) * 2006-07-28 2009-06-30 씨제이제일제당 (주) Microorganism producing l-methionine precursor and method of producing l-methionine and organic acid from the l-methionine precurosr
BRPI0807760A2 (en) 2007-02-19 2014-06-17 Evonik Degussa Gmbh CORINEFORM BACTERIA WITH FORMAT-THF SYNTETASE ACTIVITY AND / OR GLYCIN CLIVING ACTIVITY
KR100858913B1 (en) * 2007-03-09 2008-09-17 한국과학기술원 L-threonine Overproducing Microorganism Which Constructed by Specific Genetic Modifications and Method for Preparing ??threonine Using the Same
WO2009043372A1 (en) 2007-10-02 2009-04-09 Metabolic Explorer Increasing methionine yield
WO2010020290A1 (en) * 2008-08-22 2010-02-25 Metabolic Explorer Producing methionine without n-acetyl methionine
EP2519637A2 (en) * 2009-12-30 2012-11-07 Metabolic Explorer Strains and method for the production of methionine

Also Published As

Publication number Publication date
EP2519637A2 (en) 2012-11-07
KR20120114325A (en) 2012-10-16
US9382561B2 (en) 2016-07-05
BR112012016908B1 (en) 2020-03-31
MY172018A (en) 2019-11-12
WO2011080301A2 (en) 2011-07-07
KR101915819B1 (en) 2018-11-06
AR079821A1 (en) 2012-02-22
US20120288904A1 (en) 2012-11-15
BR112012016908A2 (en) 2015-09-15
MX2012007717A (en) 2012-07-25
JP2013516162A (en) 2013-05-13
CN102782137A (en) 2012-11-14
CN102782137B (en) 2016-01-06
WO2011080301A3 (en) 2012-04-12
JP5922036B2 (en) 2016-05-24

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