IL42119A - Purification of carcinoembryonic antigen - Google Patents
Purification of carcinoembryonic antigenInfo
- Publication number
- IL42119A IL42119A IL42119A IL4211973A IL42119A IL 42119 A IL42119 A IL 42119A IL 42119 A IL42119 A IL 42119A IL 4211973 A IL4211973 A IL 4211973A IL 42119 A IL42119 A IL 42119A
- Authority
- IL
- Israel
- Prior art keywords
- carcinoembryonic antigen
- sodium chloride
- cellulose
- particle size
- human carcinoembryonic
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J47/00—Ion-exchange processes in general; Apparatus therefor
- B01J47/02—Column or bed processes
- B01J47/04—Mixed-bed processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
- B01J39/26—Cation exchangers for chromatographic processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J47/00—Ion-exchange processes in general; Apparatus therefor
- B01J47/014—Ion-exchange processes in general; Apparatus therefor in which the adsorbent properties of the ion-exchanger are involved, e.g. recovery of proteins or other high-molecular compounds
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57565—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving carcinoembryonic antigen [CEA]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Claims (7)
1. A process for the purification of human carcinoembryonic antigen which comprises subjecting carcinoembryonic antigen material to chromatography on a mixed bed cellulose ion exchange column comprising microgranular diethylamino-ethyl cellulose in the free base form having rod shaped particles, a capacity of 1.0 + 0.1 meq/gm, a particle size distribution of about 20 μ to about 60 μ and a water regain of 2.3 to 2.8 gm/gm dry exchanger, and a microgranular carboxymethyl cellulose with a capacity of 1.0 + 0.1 meq/gm, having rod shaped particles, a particle size distribution of about 20 μ to about 60 μ and a water regain of 2.3 to 2.7 gm/gm dry exchanger, and collecting the eluates resulting from eluting with a solution of ammonium acetate in sodium chloride followed by subjecting the resulting material to sequential chromatography on two different gel columns, the first of which is an agarose gel having about 6 o by weight agarose and a particle size of from 40-210 microns and the second of which is a hydrophilic water-insoluble cross-linked dextran polymer gel and collecting and concentrating the eluates which have a spectrophotometric absorption peak wave length of 280 ιημ and a molecular weight of about 200,000 to 500,000.
2. A process as claimed in claim 1 wherein the mixed bed column contains equal amounts by weight of the diethyl-aminoethyl cellulose and carboxymethyl cellulose.
3. A process as claimed in claim 1 or claim 2 wherein the solution of ammonium acetate in sodium chloride has a pH of 4.
4. A process as claimed in any one of claims 1 to 3 for the production of human carcinoembryonic antigen component A wherein the sodium chloride has a molarity of 0.05 M.
5. A process as claimed in any one of claims 1 to 3 for the production of human carcinoembryonic antigen i component B wherein the sodium chloride has a molarity 0.1 .
6. ■ 6. A process as claimed in claim 1 substantially as hereinbefore described with particular reference to the foregoing Example . I
7. Human carcinoembryonic antigen, whenever purified by a process as claimed in any one of the preceding claims.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US252700A US3867363A (en) | 1970-06-01 | 1972-05-12 | Carcinoembryonic antigens |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL42119A0 IL42119A0 (en) | 1973-06-29 |
| IL42119A true IL42119A (en) | 1976-08-31 |
Family
ID=22957148
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL42119A IL42119A (en) | 1972-05-12 | 1973-04-27 | Purification of carcinoembryonic antigen |
Country Status (15)
| Country | Link |
|---|---|
| JP (1) | JPS5912990B2 (en) |
| AU (1) | AU468779B2 (en) |
| BE (1) | BE799405A (en) |
| CA (1) | CA948991A (en) |
| CH (1) | CH583036A5 (en) |
| DD (1) | DD107293A5 (en) |
| DE (1) | DE2323955A1 (en) |
| DK (1) | DK133559B (en) |
| FR (1) | FR2184753B1 (en) |
| GB (1) | GB1378134A (en) |
| IL (1) | IL42119A (en) |
| IT (1) | IT998101B (en) |
| NL (1) | NL7306346A (en) |
| SE (1) | SE403912B (en) |
| ZA (1) | ZA732770B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4146603A (en) * | 1977-02-18 | 1979-03-27 | Research Corporation | Tumor specific glycoproteins and method for detecting tumorigenic cancers |
| GB2122641B (en) * | 1982-06-07 | 1986-08-06 | Otto A Gansow | Metal chelate conjugated monoclonal antibodies where in the metal is an emitter |
| JPS6076395A (en) * | 1983-10-03 | 1985-04-30 | Dainippon Screen Mfg Co Ltd | Eluting device for printing plate |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3697638A (en) * | 1970-06-01 | 1972-10-10 | Hoffmann La Roche | Antigens |
-
1973
- 1973-04-11 CH CH519673A patent/CH583036A5/xx not_active IP Right Cessation
- 1973-04-20 IT IT23343/73A patent/IT998101B/en active
- 1973-04-24 ZA ZA732770A patent/ZA732770B/en unknown
- 1973-04-25 CA CA169,501A patent/CA948991A/en not_active Expired
- 1973-04-27 IL IL42119A patent/IL42119A/en unknown
- 1973-04-30 AU AU54989/73A patent/AU468779B2/en not_active Expired
- 1973-05-07 DK DK249773AA patent/DK133559B/en not_active IP Right Cessation
- 1973-05-07 NL NL7306346A patent/NL7306346A/xx unknown
- 1973-05-10 JP JP48051263A patent/JPS5912990B2/en not_active Expired
- 1973-05-10 DD DD170753A patent/DD107293A5/xx unknown
- 1973-05-11 GB GB2261173A patent/GB1378134A/en not_active Expired
- 1973-05-11 FR FR7317188A patent/FR2184753B1/fr not_active Expired
- 1973-05-11 BE BE131003A patent/BE799405A/en not_active IP Right Cessation
- 1973-05-11 DE DE2323955A patent/DE2323955A1/en active Pending
- 1973-05-11 SE SE7306698-7A patent/SE403912B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| AU468779B2 (en) | 1976-01-22 |
| DE2323955A1 (en) | 1973-11-22 |
| DK133559C (en) | 1977-02-21 |
| BE799405A (en) | 1973-11-12 |
| SE403912B (en) | 1978-09-11 |
| IT998101B (en) | 1976-01-20 |
| DK133559B (en) | 1976-06-08 |
| FR2184753A1 (en) | 1973-12-28 |
| ZA732770B (en) | 1974-04-24 |
| JPS4947521A (en) | 1974-05-08 |
| FR2184753B1 (en) | 1977-11-04 |
| CH583036A5 (en) | 1976-12-31 |
| NL7306346A (en) | 1973-11-14 |
| IL42119A0 (en) | 1973-06-29 |
| GB1378134A (en) | 1974-12-18 |
| CA948991A (en) | 1974-06-11 |
| DD107293A5 (en) | 1974-07-20 |
| JPS5912990B2 (en) | 1984-03-27 |
| AU5498973A (en) | 1974-10-31 |
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