IL312893A - Crispr/cas screening platform to identify genetic modifiers of tau seeding or aggregation - Google Patents

Crispr/cas screening platform to identify genetic modifiers of tau seeding or aggregation

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Publication number
IL312893A
IL312893A IL312893A IL31289324A IL312893A IL 312893 A IL312893 A IL 312893A IL 312893 A IL312893 A IL 312893A IL 31289324 A IL31289324 A IL 31289324A IL 312893 A IL312893 A IL 312893A
Authority
IL
Israel
Prior art keywords
cells
population
aggregation
tau
guide rna
Prior art date
Application number
IL312893A
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English (en)
Hebrew (he)
Original Assignee
Regeneron Pharma
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Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of IL312893A publication Critical patent/IL312893A/en

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
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    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
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    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • G01N33/6896—Neurological disorders, e.g. Alzheimer's disease
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2320/00—Applications; Uses
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IL312893A 2019-03-18 2020-03-17 Crispr/cas screening platform to identify genetic modifiers of tau seeding or aggregation IL312893A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962820086P 2019-03-18 2019-03-18
PCT/US2020/023131 WO2020190932A1 (en) 2019-03-18 2020-03-17 Crispr/cas screening platform to identify genetic modifiers of tau seeding or aggregation

Publications (1)

Publication Number Publication Date
IL312893A true IL312893A (en) 2024-07-01

Family

ID=70285889

Family Applications (2)

Application Number Title Priority Date Filing Date
IL312893A IL312893A (en) 2019-03-18 2020-03-17 Crispr/cas screening platform to identify genetic modifiers of tau seeding or aggregation
IL286357A IL286357B2 (en) 2019-03-18 2020-03-17 Crispr/cas screening platform to identify genetic modifiers of tau seeding or aggregation

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Application Number Title Priority Date Filing Date
IL286357A IL286357B2 (en) 2019-03-18 2020-03-17 Crispr/cas screening platform to identify genetic modifiers of tau seeding or aggregation

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Country Link
US (4) US11845931B2 (enExample)
EP (3) EP4729947A3 (enExample)
JP (3) JP7461368B2 (enExample)
KR (3) KR20260032649A (enExample)
CN (2) CN120648640A (enExample)
AU (2) AU2020241858B2 (enExample)
CA (1) CA3127813A1 (enExample)
ES (2) ES2970541T3 (enExample)
IL (2) IL312893A (enExample)
SG (1) SG11202108091YA (enExample)
WO (1) WO2020190932A1 (enExample)

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CN113906134B (zh) 2019-06-14 2025-06-24 瑞泽恩制药公司 Tau蛋白病模型
WO2023046996A1 (en) * 2021-09-27 2023-03-30 Cemm - Forschungszentrum Für Molekulare Medizin Gmbh Method for improved intron tagging and automated clone recognition

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