IL297008A - שיטות והרכבים לייצור קסיליטול מקסילוז באמצעות שליטה מטבוליטית דינמית - Google Patents

שיטות והרכבים לייצור קסיליטול מקסילוז באמצעות שליטה מטבוליטית דינמית

Info

Publication number
IL297008A
IL297008A IL297008A IL29700822A IL297008A IL 297008 A IL297008 A IL 297008A IL 297008 A IL297008 A IL 297008A IL 29700822 A IL29700822 A IL 29700822A IL 297008 A IL297008 A IL 297008A
Authority
IL
Israel
Prior art keywords
microorganism
genetically modified
xylose
xylitol
synthetic metabolic
Prior art date
Application number
IL297008A
Other languages
English (en)
Inventor
Michael David Lynch
Shuai Li
Original Assignee
Univ Duke
Michael David Lynch
Shuai Li
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Duke, Michael David Lynch, Shuai Li filed Critical Univ Duke
Publication of IL297008A publication Critical patent/IL297008A/he

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01049—Glucose-6-phosphate dehydrogenase (1.1.1.49)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01307—D-Xylose reductase (1.1.1.307)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
    • C12Y103/0101—Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific)(1.3.1.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01005—Xylose isomerase (5.3.1.5)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
IL297008A 2020-04-03 2021-04-02 שיטות והרכבים לייצור קסיליטול מקסילוז באמצעות שליטה מטבוליטית דינמית IL297008A (he)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063004740P 2020-04-03 2020-04-03
PCT/US2021/025487 WO2021242408A2 (en) 2020-04-03 2021-04-02 Methods and compositions for the production of xylitol from xylose utilizing dynamic metabolic control

Publications (1)

Publication Number Publication Date
IL297008A true IL297008A (he) 2022-12-01

Family

ID=78745743

Family Applications (1)

Application Number Title Priority Date Filing Date
IL297008A IL297008A (he) 2020-04-03 2021-04-02 שיטות והרכבים לייצור קסיליטול מקסילוז באמצעות שליטה מטבוליטית דינמית

Country Status (9)

Country Link
JP (1) JP2023528727A (he)
KR (1) KR20220164007A (he)
CN (1) CN115916990A (he)
AU (1) AU2021278792B2 (he)
BR (1) BR112022019942A2 (he)
CA (1) CA3179180A1 (he)
IL (1) IL297008A (he)
PH (1) PH12022552636A1 (he)
WO (1) WO2021242408A2 (he)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023192368A1 (en) * 2022-03-30 2023-10-05 The Regents Of The University Of California Hydrogelated cells
CN118599751B (zh) * 2024-07-03 2025-02-28 湖北大学 大肠杆菌的重组菌株、方法及应用
CN118685438B (zh) * 2024-08-09 2025-03-04 微元合成生物技术(北京)有限公司 一种基于nad(h)辅因子循环系统合成d-木糖醇的方法及其专用工程菌

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5747281A (en) * 1984-09-28 1998-05-05 Cornell Research Foundation, Inc. System useful for the production of proteins from recombinant DNA in single celled organisms
FR2862068B1 (fr) * 2003-11-06 2007-10-12 Metabolic Explorer Sa Souches de microorganismes optimisees pour des voies de biosyntheses consommatrices de nadph
US20140322779A1 (en) * 2011-11-11 2014-10-30 Genomatica, Inc. Eukaryotic organisms and methods for increasing the availability of cytosolic acetyl-coa, and for the producing 1,3-butanediol
HUE061007T2 (hu) * 2014-06-11 2023-05-28 Univ Duke Gyors és dinamikus áramlásszabályozásra szolgáló összetételek és módszerek szintetikus matabolikus szelepek segítségével
EP3225691B1 (en) * 2014-11-28 2020-10-28 Ajinomoto Co., Inc. Method for producing isoprenoid compound
CN104593308B (zh) * 2014-12-09 2017-07-14 浙江大学 一种基因工程菌及其构建方法和在生产木糖醇中的应用
EP3585878A4 (en) * 2017-02-21 2020-12-09 Duke University COMPOSITIONS AND PROCESSES FOR ROBUST DYNAMIC METABOLIC REGULATION
US11203744B2 (en) * 2018-06-21 2021-12-21 Duke University Compositions and methods for the production of pyruvic acid and related products using dynamic metabolic control

Also Published As

Publication number Publication date
PH12022552636A1 (en) 2024-01-22
KR20220164007A (ko) 2022-12-12
AU2021278792B2 (en) 2026-02-12
BR112022019942A2 (pt) 2022-12-13
CA3179180A1 (en) 2021-12-02
AU2021278792A1 (en) 2022-11-03
WO2021242408A3 (en) 2022-04-07
JP2023528727A (ja) 2023-07-06
WO2021242408A2 (en) 2021-12-02
CN115916990A (zh) 2023-04-04

Similar Documents

Publication Publication Date Title
Cui et al. Reconfiguration of the reductive TCA cycle enables high-level succinic acid production by Yarrowia lipolytica
Guarnieri et al. Conversion and assimilation of furfural and 5-(hydroxymethyl) furfural by Pseudomonas putida KT2440
Knietsch et al. Construction and screening of metagenomic libraries derived from enrichment cultures: generation of a gene bank for genes conferring alcohol oxidoreductase activity on Escherichia coli
Nozzi et al. Systematic approaches to efficiently produce 2, 3-butanediol in a marine cyanobacterium
Bao et al. Regulation of the NADH pool and NADH/NADPH ratio redistributes acetoin and 2, 3‐butanediol proportion in Bacillus subtilis
IL297008A (he) שיטות והרכבים לייצור קסיליטול מקסילוז באמצעות שליטה מטבוליטית דינמית
Dev et al. Adaptation on xylose improves glucose–xylose co-utilization and ethanol production in a carbon catabolite repression (CCR) compromised ethanologenic strain
IL296347A (he) תכשירים ושיטות לבקרה מטבולית דינמית חסונה
Mindt et al. Fermentative production of N-methylglutamate from glycerol by recombinant Pseudomonas putida
Guo et al. Efficient (3R)-acetoin production from meso-2, 3-butanediol using a new whole-cell biocatalyst with co-expression of meso-2, 3-butanediol dehydrogenase, NADH oxidase, and Vitreoscilla hemoglobin
Sathesh-Prabu et al. Metabolic engineering of Escherichia coli for 2, 3-butanediol production from cellulosic biomass by using glucose-inducible gene expression system
Akita et al. Bacterial production of isobutanol without expensive reagents
Yuan et al. Enhancing intracellular NADPH bioavailability through improving pentose phosphate pathway flux and its application in biocatalysis asymmetric reduction reaction
Zahid et al. Role of mannitol dehydrogenases in osmoprotection of Gluconobacter oxydans
Wang et al. Engineering the Cad pathway in Escherichia coli to produce glutarate from L-lysine
Gao et al. High-yield production of D-1, 2, 4-butanetriol from lignocellulose-derived xylose by using a synthetic enzyme cascade in a cell-free system
Shimizu et al. A mycofactocin-associated dehydrogenase is essential for ethylene glycol metabolism by Rhodococcus jostii RHA1
Wang et al. Discovery of a new NADPH-dependent aldo-keto reductase from Candida orthopsilosis catalyzing the stereospecific synthesis of (R)-pantolactone by genome mining
US20140248687A1 (en) Methods for expressing polypeptides in hyperthermophiles
Melero‐Cobo et al. MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering
Ban et al. Identification of a vitamin D3-specific hydroxylase genes through actinomycetes genome mining
Wu et al. Intensifying niacin-based biosynthesis of NAD+ to enhance 3-hydroxypropionic acid production in Klebsiella pneumoniae
Ma et al. Development of an efficient recombinant protein expression system in Clostridium saccharoperbutylacetonicum based on the bacteriophage T7 system
Qin et al. High-efficiency secretion expression of cellobiose 2-epimerase in Escherichia coli and its applications
Yamamura Bioconversion of pyridoxine to pyridoxamine through pyridoxal using a Rhodococcus expression system