IL289421A - Identification, characterization and quantification of DNA double-strand break repairs introduced by crispr - Google Patents
Identification, characterization and quantification of DNA double-strand break repairs introduced by crisprInfo
- Publication number
- IL289421A IL289421A IL289421A IL28942121A IL289421A IL 289421 A IL289421 A IL 289421A IL 289421 A IL289421 A IL 289421A IL 28942121 A IL28942121 A IL 28942121A IL 289421 A IL289421 A IL 289421A
- Authority
- IL
- Israel
- Prior art keywords
- crispr
- quantitation
- characterization
- identification
- stranded dna
- Prior art date
Links
- 238000012512 characterization method Methods 0.000 title 1
- 230000008439 repair process Effects 0.000 title 1
- 230000003007 single stranded DNA break Effects 0.000 title 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
- G16B20/30—Detection of binding sites or motifs
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
- G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
- G16B30/10—Sequence alignment; Homology search
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
- G16B30/20—Sequence assembly
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
- G16B40/20—Supervised data analysis
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics
- G16B50/30—Data warehousing; Computing architectures
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Medical Informatics (AREA)
- Theoretical Computer Science (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Evolutionary Biology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Data Mining & Analysis (AREA)
- Databases & Information Systems (AREA)
- Bioethics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Epidemiology (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Software Systems (AREA)
- Artificial Intelligence (AREA)
- Evolutionary Computation (AREA)
- Public Health (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962870471P | 2019-07-03 | 2019-07-03 | |
| US201962870426P | 2019-07-03 | 2019-07-03 | |
| US201962952598P | 2019-12-23 | 2019-12-23 | |
| US201962952603P | 2019-12-23 | 2019-12-23 | |
| PCT/US2020/040621 WO2021003343A1 (en) | 2019-07-03 | 2020-07-02 | Identification, characterization, and quantitation of crispr-introduced double-stranded dna break repairs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL289421A true IL289421A (en) | 2022-02-01 |
Family
ID=74065411
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL289421A IL289421A (en) | 2019-07-03 | 2021-12-27 | Identification, characterization and quantification of DNA double-strand break repairs introduced by crispr |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US12254959B2 (cg-RX-API-DMAC10.html) |
| EP (1) | EP3994258A4 (cg-RX-API-DMAC10.html) |
| JP (2) | JP7579817B2 (cg-RX-API-DMAC10.html) |
| AU (1) | AU2020301025B2 (cg-RX-API-DMAC10.html) |
| CA (1) | CA3144106A1 (cg-RX-API-DMAC10.html) |
| IL (1) | IL289421A (cg-RX-API-DMAC10.html) |
| WO (1) | WO2021003343A1 (cg-RX-API-DMAC10.html) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3154370A1 (en) | 2019-10-24 | 2021-04-29 | Jessica WOODLEY | Modified double-stranded donor templates |
| CN119343462A (zh) | 2022-05-13 | 2025-01-21 | 合成Dna技术公司 | 使用独特分子标识符改善长读测序的准确性和crispr编辑的表征 |
| US20260049352A1 (en) | 2024-08-14 | 2026-02-19 | Integrated Dna Technologies, Inc. | Adaptered-tag blocking oligonucleotides |
Family Cites Families (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9809904B2 (en) | 2011-04-21 | 2017-11-07 | University Of Washington Through Its Center For Commercialization | Methods for retrieval of sequence-verified DNA constructs |
| CN105531375B (zh) | 2012-12-10 | 2020-03-03 | 分析生物科学有限公司 | 靶向基因组分析的方法 |
| AU2013381709A1 (en) | 2013-03-15 | 2015-10-01 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| US11306328B2 (en) | 2013-07-26 | 2022-04-19 | President And Fellows Of Harvard College | Genome engineering |
| WO2015113063A1 (en) * | 2014-01-27 | 2015-07-30 | Georgia Tech Research Corporation | Methods and systems for identifying crispr/cas off-target sites |
| AU2015280069B2 (en) | 2014-06-23 | 2021-08-12 | The General Hospital Corporation | Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq) |
| WO2016030899A1 (en) | 2014-08-28 | 2016-03-03 | Yeda Research And Development Co. Ltd. | Methods of treating amyotrophic lateral scleroses |
| WO2016081798A1 (en) | 2014-11-20 | 2016-05-26 | Children's Medical Center Corporation | Methods relating to the detection of recurrent and non-specific double strand breaks in the genome |
| EP3234192B1 (en) * | 2014-12-19 | 2021-07-14 | The Broad Institute, Inc. | Unbiased identification of double-strand breaks and genomic rearrangement by genome-wide insert capture sequencing |
| EP3262189B1 (en) | 2015-02-27 | 2021-12-08 | Becton, Dickinson and Company | Methods for barcoding nucleic acids for sequencing |
| US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| CA2989445A1 (en) | 2015-06-22 | 2016-12-29 | Mcmaster University | Biosensor comprising tandem reactions of structure switching, nucleolytic digestion and amplification of a nucleic acid assembly |
| US10526590B2 (en) | 2015-08-31 | 2020-01-07 | Agilent Technologies, Inc. | Compounds and methods for CRISPR/Cas-based genome editing by homologous recombination |
| US20170081679A1 (en) | 2015-09-22 | 2017-03-23 | Jie Xu | Methods of improving nuclease mediated homologous recombination |
| JP7011590B2 (ja) | 2015-10-12 | 2022-02-10 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー | 細胞内での遺伝子組換えおよび相同組換えの増加のための保護ｄｎａ鋳型および使用方法 |
| CN116814590A (zh) | 2015-10-22 | 2023-09-29 | 布罗德研究所有限公司 | Vi-b型crispr酶和系统 |
| US20170145486A1 (en) | 2015-11-25 | 2017-05-25 | Integrated Dna Technologies, Inc. | Methods for variant detection |
| GB201602473D0 (en) | 2016-02-11 | 2016-03-30 | Horizon Discovery Ltd | Replicative transposon system |
| US11810649B2 (en) | 2016-08-17 | 2023-11-07 | The Broad Institute, Inc. | Methods for identifying novel gene editing elements |
| CN118460529A (zh) | 2016-12-20 | 2024-08-09 | 百时美施贵宝公司 | 提高细胞基因组中同源定向修复(hdr)效率的方法 |
| CN111032867A (zh) | 2017-06-16 | 2020-04-17 | 应用干细胞有限公司 | 具有提高的敲入效率的基因编辑方法 |
| US20190062736A1 (en) * | 2017-08-22 | 2019-02-28 | Board Of Regents, The University Of Texas System | In situ and in vivo analysis of chromatin interactions by biotinylated dcas9 protein |
| CN111315889A (zh) | 2017-09-08 | 2020-06-19 | 生命技术公司 | 提高同源重组的方法和其组合物 |
| WO2019110067A1 (en) | 2017-12-07 | 2019-06-13 | Aarhus Universitet | Hybrid nanoparticle |
| US12406749B2 (en) | 2017-12-15 | 2025-09-02 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| EP3770256A4 (en) | 2018-03-20 | 2021-12-22 | Tokyo Institute of Technology | ANTISENSE OLIGONUCLEOTIDE WITH REDUCED TOXICITY |
| WO2019246553A1 (en) * | 2018-06-21 | 2019-12-26 | Memorial Sloan Kettering Cancer Center | Compositions & methods for monitoring dna repair |
| WO2020178772A1 (en) | 2019-03-04 | 2020-09-10 | King Abdullah University Of Science And Technology | Compositions and methods of labeling nucleic acids and sequencing and analysis thereof |
| CA3154370A1 (en) | 2019-10-24 | 2021-04-29 | Jessica WOODLEY | Modified double-stranded donor templates |
-
2020
- 2020-07-02 EP EP20835024.9A patent/EP3994258A4/en active Pending
- 2020-07-02 WO PCT/US2020/040621 patent/WO2021003343A1/en not_active Ceased
- 2020-07-02 US US16/919,577 patent/US12254959B2/en active Active
- 2020-07-02 AU AU2020301025A patent/AU2020301025B2/en active Active
- 2020-07-02 CA CA3144106A patent/CA3144106A1/en active Pending
- 2020-07-02 JP JP2021577412A patent/JP7579817B2/ja active Active
-
2021
- 2021-12-27 IL IL289421A patent/IL289421A/en unknown
-
2024
- 2024-10-28 JP JP2024188978A patent/JP7898488B2/ja active Active
-
2025
- 2025-02-12 US US19/052,059 patent/US20250218536A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP7579817B2 (ja) | 2024-11-08 |
| AU2020301025A1 (en) | 2022-01-20 |
| US12254959B2 (en) | 2025-03-18 |
| CA3144106A1 (en) | 2021-01-07 |
| AU2020301025B2 (en) | 2026-07-02 |
| JP7898488B2 (ja) | 2026-07-31 |
| JP2025014002A (ja) | 2025-01-28 |
| JP2022540792A (ja) | 2022-09-20 |
| US20210002700A1 (en) | 2021-01-07 |
| EP3994258A1 (en) | 2022-05-11 |
| EP3994258A4 (en) | 2022-10-26 |
| WO2021003343A1 (en) | 2021-01-07 |
| US20250218536A1 (en) | 2025-07-03 |
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