IL286327B2 - תאים דיסקוגניים מבודדים, שיטות לשימוש בהם ושיטות להכנתם מרקמת יונקים - Google Patents

תאים דיסקוגניים מבודדים, שיטות לשימוש בהם ושיטות להכנתם מרקמת יונקים

Info

Publication number
IL286327B2
IL286327B2 IL286327A IL28632721A IL286327B2 IL 286327 B2 IL286327 B2 IL 286327B2 IL 286327 A IL286327 A IL 286327A IL 28632721 A IL28632721 A IL 28632721A IL 286327 B2 IL286327 B2 IL 286327B2
Authority
IL
Israel
Prior art keywords
cells
discogenic
culture
cell population
cell
Prior art date
Application number
IL286327A
Other languages
English (en)
Other versions
IL286327B1 (he
IL286327A (he
Original Assignee
Discgenics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Discgenics Inc filed Critical Discgenics Inc
Publication of IL286327A publication Critical patent/IL286327A/he
Publication of IL286327B1 publication Critical patent/IL286327B1/he
Publication of IL286327B2 publication Critical patent/IL286327B2/he

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/30—Joints
    • A61F2/44—Joints for the spine, e.g. vertebrae, spinal discs
    • A61F2/442—Intervertebral or spinal discs, e.g. resilient
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/32—Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0658—Skeletal muscle cells, e.g. myocytes, myotubes, myoblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Rheumatology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Neurology (AREA)
  • Epidemiology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Vascular Medicine (AREA)
  • Cardiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Transplantation (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
IL286327A 2013-03-15 2014-03-14 תאים דיסקוגניים מבודדים, שיטות לשימוש בהם ושיטות להכנתם מרקמת יונקים IL286327B2 (he)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361794691P 2013-03-15 2013-03-15
PCT/US2014/028026 WO2014143870A1 (en) 2013-03-15 2014-03-14 Isolated discogenic cells, methods of using, and methods of preparing same from mammalian tissue

Publications (3)

Publication Number Publication Date
IL286327A IL286327A (he) 2021-10-31
IL286327B1 IL286327B1 (he) 2025-09-01
IL286327B2 true IL286327B2 (he) 2026-01-01

Family

ID=50680157

Family Applications (2)

Application Number Title Priority Date Filing Date
IL286327A IL286327B2 (he) 2013-03-15 2014-03-14 תאים דיסקוגניים מבודדים, שיטות לשימוש בהם ושיטות להכנתם מרקמת יונקים
IL241061A IL241061B2 (he) 2013-03-15 2015-09-02 תאים דיסקוגניים מבודדים, שיטות לשימוש בהם ושיטות להכנתם מרקמת יונקים

Family Applications After (1)

Application Number Title Priority Date Filing Date
IL241061A IL241061B2 (he) 2013-03-15 2015-09-02 תאים דיסקוגניים מבודדים, שיטות לשימוש בהם ושיטות להכנתם מרקמת יונקים

Country Status (11)

Country Link
US (2) US11891626B2 (he)
EP (2) EP4461808A3 (he)
JP (6) JP2016515379A (he)
KR (4) KR20250041194A (he)
CN (2) CN111363719B (he)
AU (2) AU2014228090B2 (he)
CA (1) CA2904138C (he)
IL (2) IL286327B2 (he)
MX (1) MX383334B (he)
SG (2) SG11201507091SA (he)
WO (1) WO2014143870A1 (he)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4461808A3 (en) * 2013-03-15 2025-03-12 DiscGenics, Inc. Isolated discogenic cells, methods of using, and methods of preparing same from mammalian tissue
EP3043825A4 (en) 2013-09-09 2017-05-03 Figene, LLC Gene therapy for the regeneration of chondrocytes or cartilage type cells
US10136958B2 (en) 2014-10-28 2018-11-27 Amendia, Inc. Tissue protector and method of use
CN106031792A (zh) * 2015-03-10 2016-10-19 西比曼生物科技（上海）有限公司 治疗关节软骨缺损的组合物
JP2019501943A (ja) * 2016-01-14 2019-01-24 スパイナルサイト， エルエルシー 軟骨細胞または軟骨型細胞を再生するための細胞混成物
US10645921B2 (en) 2016-12-20 2020-05-12 Vivex Biologics Group, Inc. Viable disc regenerative composition and method of manufacture and use
JP6712740B1 (ja) * 2019-03-25 2020-06-24 学校法人東海大学 Ｔｉｅ２陽性幹／前駆細胞を含む細胞集団の培養方法およびその利用
WO2021039882A1 (ja) * 2019-08-28 2021-03-04 学校法人東海大学 培養基材を用いたＴｉｅ２陽性幹／前駆細胞を含む細胞集団の培養方法およびその利用
JP6905234B2 (ja) * 2019-09-09 2021-07-21 学校法人東海大学 Ｔｉｅ２陽性幹／前駆細胞を含む細胞集団の培養方法およびその利用
JP6779492B2 (ja) * 2019-09-09 2020-11-04 学校法人東海大学 Ｔｉｅ２陽性幹／前駆細胞を含む細胞集団の培養方法およびその利用
JP7421182B2 (ja) * 2020-04-28 2024-01-24 学校法人東海大学 Ｔｉｅ２陽性幹／前駆細胞を含む細胞集団の培養方法およびその利用
CN113088482B (zh) * 2021-04-16 2022-12-23 四川农业大学 一种犊牛瘤胃上皮细胞分离培养的方法
WO2022266262A1 (en) * 2021-06-15 2022-12-22 Discgenics, Inc. Novel methods for production of therapeutic mammalian cells and cell spheres and compositions of same
TW202532652A (zh) * 2024-02-06 2025-08-16 精準再生生醫股份有限公司 髓核前驅細胞的分離與鑑別方法及其用途
WO2026068839A1 (en) * 2024-09-30 2026-04-02 Bluu Gmbh Large scale fish cell production

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030165473A1 (en) 2001-11-09 2003-09-04 Rush-Presbyterian-St. Luke's Medical Center Engineered intervertebral disc tissue
US20030220692A1 (en) * 2002-02-09 2003-11-27 Shapiro Irving M. Preparations of nucleus pulposus cells and methods for their generation, identification, and use
US8148150B2 (en) 2003-12-12 2012-04-03 Co.Don Ag Method for the production of intervertebral disk cell transplants and their use as transplantation material
AU2006249302A1 (en) * 2005-05-27 2006-11-30 Emory University Chondrogenic compositions and methods of use
US8734827B2 (en) * 2006-04-28 2014-05-27 University Of Hong Kong Bioengineered intervertebral discs and methods for their preparation
US8679809B2 (en) * 2006-05-19 2014-03-25 The University Of Hong Kong Cell-matrix microspheres, methods for preparation and applications
JP5509073B2 (ja) * 2007-07-12 2014-06-04 ディスクジェニクス ヒト円板組織
US20120100607A1 (en) * 2007-07-12 2012-04-26 Christopher Duntsch Compositions of adult disc stem cells and methods for the treatment of degenerative disc disease
US20090183503A1 (en) * 2008-01-18 2009-07-23 Alberto Verdesi Exhaust apparatus
CN105796601A (zh) * 2009-07-16 2016-07-27 生物时代股份有限公司 用于体外和体内软骨发生的方法和组合物
EP2554660A1 (en) 2010-03-30 2013-02-06 Tokai University Educational System Intervertebral disc nucleus pulposus stem/progenitor cell, method for culturing same, and application
EP4461808A3 (en) 2013-03-15 2025-03-12 DiscGenics, Inc. Isolated discogenic cells, methods of using, and methods of preparing same from mammalian tissue
WO2014147658A1 (en) * 2013-03-18 2014-09-25 Hitachi, Ltd. Compound storage system and storage control method
JP2021092330A (ja) * 2019-12-06 2021-06-17 株式会社Ｏ’ｓ＆Ｔｅｃ 冷蔵保存庫

Also Published As

Publication number Publication date
KR102784154B1 (ko) 2025-03-19
JP2026041883A (ja) 2026-03-10
EP2970904B1 (en) 2024-08-14
JP2021129596A (ja) 2021-09-09
US11891626B2 (en) 2024-02-06
IL241061B2 (he) 2023-06-01
CA2904138A1 (en) 2014-09-18
WO2014143870A1 (en) 2014-09-18
JP2019088302A (ja) 2019-06-13
AU2014228090A1 (en) 2015-09-24
MX2015012729A (es) 2016-06-21
EP4461808A2 (en) 2024-11-13
KR20230125089A (ko) 2023-08-28
AU2020203168B2 (en) 2022-03-24
JP2024178216A (ja) 2024-12-24
US20240182861A1 (en) 2024-06-06
KR20210059047A (ko) 2021-05-24
CN105308176B (zh) 2020-04-17
AU2020203168A1 (en) 2020-06-04
KR20160034244A (ko) 2016-03-29
SG11201507091SA (en) 2015-10-29
CN105308176A (zh) 2016-02-03
IL286327B1 (he) 2025-09-01
EP4461808A3 (en) 2025-03-12
SG10201707546SA (en) 2017-10-30
CA2904138C (en) 2023-12-05
HK1217213A1 (en) 2016-12-30
JP2016515379A (ja) 2016-05-30
US20140286912A1 (en) 2014-09-25
KR20250041194A (ko) 2025-03-25
CN111363719B (zh) 2024-06-21
IL241061A0 (he) 2015-11-30
MX383334B (es) 2025-03-13
CN111363719A (zh) 2020-07-03
EP2970904A1 (en) 2016-01-20
JP2023138985A (ja) 2023-10-03
IL286327A (he) 2021-10-31
AU2014228090B2 (en) 2020-02-27

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