IL28058A - Preparation of an anti-rh gamma globulin - Google Patents

Description

PATENT ATTORNEYS 1 REINHOLD COHN MICHAEL COHN ISRAEL SHACHTER C PATENTS AND DESIGNS ORDINANCE SPECIFICATION Preparation of an globulin ORTHO corporation of the State of New of Hew do hereby declare the nature of this invention and in what manner the same is to be to be particularly described and ascertained in and by the following statement 163 plasminogen and Supernatant III consists principally o gamma globulins and minor amounts of fibrinogen and The final step of the fractionation yields Precipitate which is essentially pure gamma G globulin almost completely free of 19 S and lipids Precipitate II prepared by the the inventio an gamm The may more fully understood with ence to the following Human blood plasma is cooled to The plasma is centrifuged in a Sharpies with in at feeding at the rate 1000 per The cold insoluble precipitate is The of the natant adjusted to The methanol content of the batc is brought to the addition of 177 methanol per liter of The solution is stirred slow at for one hour and is maintained at The solution is centrifuged in a Sharpies trifuge with in at feeding at the of 1000 100 per Supernatant I is collected in bowl equipped with To each liter of Supernatant I there is with 601 of of 10 acetic and of 4 sodium aceta This brings the methan concentration to the strength to and the pH to The solution is brought to a temperature of and is stirred alowly for two The solution is then allowed to stand for 16 times weight o Preoipitate II III of To the 5 suspension added 2 volumes weight of Precipitate III of sodium A further 1 1 time ieight of Preoipitate II III of water containing of acetate buffer for each gram of Precipitate II III The acetate buffer is prepared by diluting 40 o 10 acid and 20 of 4 sodium acetate to 100 with distilled The pH of the solution is The solution is stirred slowly for one There is then added volumes times the original weight of Precipitate II III of ice water and volumes times the original weight of Precipitate II of methanol while maintaining the temperature at The solution is stirred slowly for one hour and allowed stand The solution contains methanol and has a pH of and an ionic strength The solution is centrifuged in a Sharpies trifuge with a at a temperature of and at a flow rate o 500 50 per The volume 6f Supernatant III is Precipitate III is Supernatant III treated with V 512 and is stirred for 20 The is filtered through an filter press containing An acetate buffer of pH made up as 25 sodium acetate and 3 10 acetic acid made up to 100 with distilled The acetate buffer is diluted 100 times with This solutio has an ionic strength of An Eitel filter press is precoated with the acetate buffer plus Cellte 512 gm of pad using 40 of inch of pad surface Supernatant III is filtered and to the filtrate is added of sodium chloride and of 1 bicarbonate per lite o filtered Supernatant The pH of insufficientOCRQuality

Claims (1)

1. 20 and onic strength being between and 21 gi llter and separating from the resulting liquid system a fourth i 22 precipitate and a fourth 23 adding methanol to said fourth precipitate and 24 adjusting the temperature to about the amount of said methano 25 being between and the pH being between and and the strength being between and and 27 separating from the resulting liquid system a fifth precipitate 28 a fifth 29 adding methanol to said fifth supernatant and 30 adjusting the temperature to about the amount of said methanol being between and the pH being between 32 and and the ionic strength being between and 33 and separating said resulting liquid system into a sixth precipita 34 and a sixth A method according to Claim wherein said sixth precipitate is insufficientOCRQuality