IL174172A - Multimeric compound that binds psgl-1 proteins, uses thereof and kits comprising the same - Google Patents

Description

174172/2 174172 |7'Ji I 453570 ΤΑΊΚ nnw nl»Dttn row ,PSGL-I >j¾i n jnvnpii n>i»»u i» n ipina Multimeric compound that binds PSGL-1 proteins, uses thereof and kits comprising the same Abgenomics Cooperatief U.A.

C. 165860 WO 2005/027831 PCT/US2()04/()2! 52() MODULATORS OF P-SELECTIN GLYCOPROTEIN LIGA D 1 Field of the Invention The invention relates to compositions and methods for controlling immune responses.

Background of the Invention The control of unwanted immune responses is a critical issue in the treatment of diseases such as inflammatory diseases, autoimmune diseases, transplant rejection, allergic diseases and T cell-derived cancers. The activity of overly aggressive T cells can be controlled by immunosuppression or by the induction of immunological tolerance. Tolerance is defined as a state where the immune system is made unresponsive to an antigen, whereas immunosuppression, which decreases the immune response to antigens, usually requires the continued use of medication. In organ transplantation, T cells play an essential role in the immune response to alloantigens. Current immunosuppressive regimes commonly involve the use of corticosteroid, cyclosporin or rapamycin, which block the transcription of IL-2, a key growth factor for T cells, or inhibit IL-2 dependent proliferation. However, a number of monoclonal antibodies which either act as T cell-depleting agents (e.g. CD3, CD4, CDS), or as inhibitors of the cytokine signaling or the co-stimulatory pathways of T cells (e.g. CD25, B7-1, B7-2, CD152, CTLA4) have demonstrated effectiveness in reducing the incidence of rejection with limited side effects or toxicity. Some of these agents have been shown to have some degree of success in treating autoimmune disease and in prolonging graft survival.

Apoptosis is widely believed to be of vital importance for maintaining the proper function of the immune system and a major mechanism to remove unwanted cells (Kabelitz et al. Immunol. Today 14:338-340 (1993); Raff, Nature:356:397-399 (1992)). Various signals originating from either inside or outside a cell influence the life and death of the cell. Antibodies against T cell surface molecules such as Fas (or CD95, MW = 43 kD), TNFR2 (MW = 75 l D), CD2 (MW = 45 kD) and CTLA-4 WO 2005/027831 PCT/llS2IMU/02y520 (MW = 33 kD) induce the apoptosis of T cells (Osborne, Curr. Opin. Immunol. 8:245-24S (1996); Lin et al. J. Immunol. 15S:598-603 (1997); Zhang et al. Nature:377:348-350 (1995); Lai et al. Eur. J. Immunol. 25:3243-324S (1995); Mollereau et al. J.

Immunol. 156:3184-3190 (1996); Gribben et al. Proc. Natl. Acad. Sci. USA 92:811-815 (1995)). Attempts to use Fas and TNFR2 molecules to control unwanted T cells have been hampered by the fact that these two molecules are expressed not only on immune cells, but also on several other important organ systems like liver. This expression pattern potentially limits the therapeutic application of these two antibodies (Ogasawara et al. Nature 364:S06-809 (1993); Pfeffer et al. Cell:73:457-467 (1993); Ehgelmann et al. J. Biological Chemistry 265:14497-14504 (1990)).

Selectins, integrins and immunoglobulin (Ig) superfamily members are three major classes of adhesion molecules that are important to the interaction of leulcocytes and platelets either with themselves or with the extracellular matrix and vascular endothelium (Springer, Nature 346:425 (1990); Osbom, Cell 62:3 (1990); Hynes, Cell 69:11 (1992)). An adhesion molecule on one cell type often binds to another adhesion molecule expressed on a different cell type, forming a ligand-receptor pair.

The selectin family consists of P-selectin (also known as CD62, CD62P, GMP140, and PADGEM), E-selectin (also known as ELAM-1 and CD62E), and L-selectin (also known as LECAM-1, Mel- 14, LAM-1, and CD62L). The selectins are highly homologous, composed of a 120 amino acid N-terminal lectin domain, an EGF-like domain, a variable number of multiple short consensus repeat (SCR) domains homologous to those found in complement regulatory proteins, followed by a transmembrane domain and short cytoplasmic tail (Siegelman et al., Science 243:1165-1172 (19S9); Lasky et al., Cell 56:1045-1055 (19S9); Tedder et al., J. Exp. Med. 170:123-133 (1989); Johnson et al., Cell 56: 1033-1044 (1989); Bevilacqua et al, Proc. Natl. Acad. Sci. USA S4:9238-9242 (1987), Bevilacqua et al, Science 243 :1160-1165 (1989), Bevilacqua et al, J. Clin. Invest. 91 :379-387 (1993), Camerini et al,, Nature 280:496-498 (1989)). The selectins have overlapping but distinct specificities for cell surface receptors (Bevilacqua et al., J. Clin. Invest. 91 :379-3S7 (1993); Feize, Current Opinion in Struct. Biol. 3:701-710 (1993); Berg et al., Biochem. Biophys. Res. Comm. 184:1048-1055 (1992); Foxall et al., J. Cell Biol. 174172/2 117: 895-902 (1992); Larsen et al., J. Biol. Chem. 267: 11 104-1 1 1 10 (1992); Polley et al., Proc. Natl. Acad. Sci. USA 88: 6224-6228 (1991)).

P-selectin, E-selectin, and L-selectin mediate the first leukocyte-endothelial cell and platelet-leukocyte adhesive interactions during inflammation (Bevilacqua et al., 1993, supra). All three selectins have been demonstrated to participate in an initial "rolling"interaction of leukocytes with activated endothelium (von Andrian et al., Proc. Natl. Acad. Sci. USA 88 : 7538-7542 (1991); Ley et al, Blood 77: 2553-2555 (1991); Abassi et al. , J. Clin. Invest. 92: 2719-2730 (1993); Dore et al. Blood 82: 1308-1316 (1993); Jones et al. , Biophys. J. 65: 1560-1569 (1993); Mayadas et al. Cell 74: 541-554 (1993)). P-selectin, expressed on activated platelets and endothelial cells, binds to cell surface proteins on most leukocytes (McEver et al, J. Biol. Chem.250: 9799-9804 (1984); Hsu-Lin et al, J. Biol. Chem. 264: 8121-9126 (1984)). E-selectin, expressed on cytokine-activated endothelial cells (e.g., after TNF-alpha or IL-1 stimulation for 6-8 hours) binds to cell surface proteins on most leukocytes (McEver et al, J. Clin. Invest. 100: 485-492 (1997); Bevilacqua et al, 1987, supra; Bevilacqua et al, 1989, supra). L-selectin, expressed on most leukocytes, binds to cell surface proteins on some endothelial cells and on other leukocytes (Gallatin et al. Nature 304: 30-34 (1983) ; Berg et al, Immunol. Rev. 108: 5-18 (1989); Berg et al, J. Cell. Biol. 114: 343-349 (1991), Hallman et al, Biochem. Biophys. Res. Comm. 174: 236-243 (1991); Smith et al. , J. Clin. Invest. 87: 609-618 (1991); Spertini et al. , J. Immunol. 147: 2565-2573 (1991) ). All three selectins have been shown to bind to a cell surface protein, PSGL-1, whose expression is largely limited to leukocytes, and in particular T cells and NK cells. Posttranslational modifications of PSGL-1 are required for binding to P-selectin, E-selectin, and L-selectin (McEver et al. , J. Clin. Invest. , 1997, supra).

US 5,840,679 discloses a novel P-selectin ligand glycoprotein along with vectors, host cells, methods of making the P-selectin ligand protein and pharmaceutical compositions containing the P-selectin ligand protein and methods of treating inflammatory disease states characterized by P-selectin- and E-selectin-mediated intercellular adhesion.

Summary of the Invention The invention is based on the discovery that T cells can be depleted and/or induced to undergo apoptosis by the engagement of the T cell surface antigen 174172/1 P- Selectin Glycoprotein Ligand-1 (PSGL-1). T cell depletion can be particularly useful for the treatment of conditions associated with an excessive or unwanted T cell-mediated immune response or excessive or unwanted T cell 3a P- Selectin Glycoprotein Ligand-1 (PSGL-1). T cell depletion can be particularly useful for the treatment of conditions associated with an excessive or unwanted T cell- mediated immune response or excessive or unwanted T cell mediated immune response or excessive or unwanted T cell proliferation. For example, the depletion of T cells can cause the reduction or elimination of undesirable T cell activity or proliferation associated with inflammatory diseases, autoimmune diseases, transplant rejection, allergic diseases, and/or T cell-derived cancers. The invention encompasses use of modulators of PSGL-1 function to prevent or reduce a T cell-mediated immune response as well as methods of screening for modulators of PSGL-1 function. 4 The present invention provides in accordance with its first aspect a use of a multimenc compound that binds to at least two PSGL-1 proteins on the surface of a T cell or natural killer (NK) cell in the manufacture of a preparation for inducing death of the T cell or the NK cell, wherein the multimeric compound (a) comprises two polypeptide chains, each of the polypeptide chains comprising (i) a binding domain that binds to PSGL-1, and (ii) a heterologous amino acid sequence, wherein the polypeptide chains are linked via the heterologous amino acid sequence to form the multimeric compound, and (b) does not include an anti-PSGL-1 antibody or an antibody fragment that binds to PSGL-1, wherein binding of the multimeric compound to the at least two PSGL-1 proteins on the surface of the T cell or NK cell induces a signal transduction pathway that results in death of the T cell or NK cell.

The present invention also provides in accordance with its second aspect, a kit comprising: a multimeric compound that binds to at least two PSGL-1 proteins on the surface of a T cell or natural killer (NK) cell, wherein the multimeric compound (a) comprises two polypeptide chains, each of the polypeptide chains comprising (i) a binding domain that binds to PSGL-1, and (ii) a heterologous amino acid sequence, wherein the polypeptide chains are linked via the heterologous amino acid sequence to form the multimeric compound, and (b) does not include an anti -PSGL-1 antibody or an antibody fragment that binds to PSGL-1, wherein binding of the multimeric compound to the at least two PSGL-1 proteins on the surface of the T cell or NK cell induces a signal transduction pathway that results in death of the T cell or NK cell; and instructions for use of the multimeric compound to treat a condition selected from inflammation, autoimmunity, transplant rejection, an allergic condition, and a T cell cancer. 6 The present invention provides in accordance with its third aspect, a multimeric compound that binds to at least two PSGL-1 proteins on the surface of a T cell or NK cell, wherein the multimeric compound comprises two polypeptide chains, each of the polypeptide chains comprising (i) a binding domain that binds to PSGL-1, and (ii) a heterologous amino acid sequence, wherein the polypeptide chains are linked via the heterologous amino acid sequence to form the multimeric compound, for use in inducing the death of a T cell or a natural killer (NK) cell, wherein the binding of the multimeric compound to the at least two PSGL-1 proteins on the surface of the T cell or NK cell induces a signal transduction pathway that results in the death of the T cell or NK cell, and wherein said compound is not an anti-PSGL-1 antibody or an antibody fragment that binds to PSGL-1. 7 The multimeric compound can be a homo-multimeric compound or a hetero-multimeric compound. The binding domain can optionally comprise a P-Selectin extracellular domain or a PSGL-1 -binding fragment thereof, an E-Selectin extracellular domain or a PSGL-1 -binding fragment thereof, an L-Selectin extracellular domain or a PSGL-1 -binding fragment thereofa PSGL-1 binding polypeptide selected from a phage display library, or a combination of any of the above.

The heterologous amino acid sequence can optionally comprise a cell surface receptor binding region. In some embodiments, the heterologous amino acid sequence comprises an immunoglobulin heavy chain constant region. In some embodiments, the polypeptide chains are covalently linked via the heterologous amino acid sequence to form the multimeric compound. The covalent linkage may be a disulfide linkage. 8 In some embodiments, the binding of the multimeric compound to the at least two PSGL-1 proteins to induce the signal transduction pathway that results in the death of the T cell or the NK cell further comprises contacting the multimeric compound with an agent that binds to the multimeric compound via the heterologous amino acid sequence to induce cross-linking of a plurality of PSGL-1 antigens on the surface of the T cell or the NK cell.

In some embodiments, the T cell is an activated T cell. According to some embodiments, the cell is a T cell and wherein the medicament is suitable for preventing or treating a condition selected from an inflammatory disease, an autoimmune disease, transplant rejection an allergic condition and a T-cell cancer.

In some embodiments, the condition is an inflammatory disease. In some other embodiments, the condition is an autoimmune disease. The autoimmune disease may be rheumatoid arthritis, psoriasis or autoimmune diabetes.

In some further embodiments, the condition is transplant rejection. In yet some other embodiments, the condition is an allergic disease. In yet some further embodiments, the condition is a T cell cancer.

An advantage of the invention is that it can induce the depletion of T cells and/or the induction of apoptosis in T cells without causing an associated unwanted or harmful immune response. For example, in some embodiments the use of a multimeric compound described herein does not result in an unwanted elevation in the levels of inflammatory cytokines such as IL-2 or TNF-alpha. 9 Another advantage of the invention is that it causes the depletion of T cells by the use of agonistic compositions that induce apoptosis of T cells. Accordingly, the invention provides for active immunosuppressive methods rather than passive immunosuppression that results from using antagonistic compositions (e. g., antagonistic anti-PSGL-1 antibodies or antagonistic soluble selectin fragments) that act by binding immune receptors and preventing immune activation mediated by such receptors.

Another advantage of the invention is that it allows for the targeting of a cell surface protein, PSGL-1, whose expression is largely limited to leukocytes, and in particular T cells and NK cells. Therefore, the compounds described herein generally do not induce significant levels of apoptosis of other cell types such as liver cells.

The targeting of T cells and NK cells (an important CD3-cell type involved in transplantation rejection) for selective depletion, without significantly inducing life-threatening systemic cytokine responses and damaging other organ systems, is a desired characteristic of an immunosuppressive agent.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which this invention belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the invention, suitable methods and materials are described below. All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety. In case of a conflict in terminology, the present specification will control. In addition, the described materials and methods are illustrative only and are not intended to be limiting.

Passages of the description which are out of the ambit of the claims do not constitute part of the invention.

Other features and advantages of the invention will be apparent from the following detailed description and the claims.

Brief Description of the Drawings Fig. 1 depicts the results of a time-course experiment that investigated when activated T cells acquire sensitivity to TAB4 (an anti-PSGL-1 monoclonal antibody)-mediated apoptotic signals.

Fig. 2 depicts tile results of cell surface biotinylation and immunoprecipitation of the antigen recognized by the TAB4 antibody.

Fig. 3 depicts the expression of the PSGL 1 antigen on spleen CD4+ T cells, CD8+ T cells, CD 19+ B cells, and NK cells.

Fig. 4 depicts the expression of the PSGL-1 antigen on CD4+, CDS+, and CD4+S+, and CD4"8" thymocytes.

Fig. 5 depicts the levels of IL-2 produced in mixed lymphocyte culture using spleen cells isolated from TAB4 (or hamster Ig)-treated B lb/c mice as the responders and H2 -mismatched C3H spleen cells as the stimulator.

Fig. 6 depicts western blot analyses demonstrating that (A) proteins immunoprecipitated with the TAB 4 antibody can be recognized by a commercially available anti-PSGL-1 antibody and (B) preclearing of T cell lysate with anti-PSGL-1 antibody can deplete the proteins recognized by the TAB4.

Fig. 7 depicts the percentage of surviving grafts in C57BL/6 mice that received a · skin graft from Balb/c mice and were treated with an anti-PSGL-1 antibody (closed diamond) or a control antibody (open square).

Fig. 8 depicts the time course of the percentage of apoptotic T cells following the treatment of activated human peripheral blood mononuclear cells with an anti-human PSGL-1 antibody.

Fig. 9 depicts the incidence of diabetes in autoimmune non-obese diabetic (NOD) male mice that were treated with anti-PSGL-1 antibody (closed square) or a control antibody (open square). 1 1 Fig. 10 depicts the binding of mouse P-selectin, E-selectin, and L- selectin to mouse activated T cells.

Figs. 11 A-l 1C depict the induction of apoptosis of mouse activated T cells by multimeric forms of E-selectin (Fig. 11A), P-selectin (Fig. 1 IB), and L-selectin (Fig. 11C).

Fig. 12 depicts the induction of apoptosis of mouse activated T cells in vitro by the cross-linking of a soluble P-selectin-Fc fusion protein.

Detailed Description The invention is directed to methods of modulating T cell activity by modulating the function of PSGL-1 molecules residing on the surface of a T cell. Engagement of PSGL-1 with agonist compositions described herein can cause the depletion of T cells and/or induce T cells to undergo apoptosis. These agonist compositions are therefore useful as therapeutic agents for controlling immune-related conditions such as inflanmiatory diseases, autoimmune diseases, transplant rejection, allergic diseases, and/or T cell-derived cancers. The agonist compositions are also useful in causing the depletion of T cells from any biological sample where the presence or activity of T cells is not desired.

PSGL-1 Protein PSGL-1 is a cell surface adhesion molecule that is expressed on neutrophils, T and B-lymphocytes, NK cells, monocytes, dendritic cells, and primitive human CD34 hematopoietic progenitor cells. Through its ability to interact with selectins, PSGL-1 mediates the rolling of leukocytes on the endothelium and the extravasation of leukocytes into inflamed tissues. PSGL-l-mediated binding of T cells to E- and P-selectin, or migration, is differentially regulated. For instance, the appearance of CLA (cutaneous lymphocyte antigen) epitope is thought to be induced on T cells undergoing nal've to memory transition. Only activated helper 1 but not helper 2 T cells express functional PSGL-1 and it capable of migration into the inflamed area of the skin.

PSGL-1 is a sialomucin that must be specifically sialylated, fucosylated, and sulfated to bind P-selectin. The PSGL-1 molecule exists in isoforais characterized by different degree of glycosylation and sulfation sites at their N-termini. Resting peripheral blood T and B cells, lymphoid cell lines, and in viti-o activated peripheral blood T cells express similar level of PGSL-1. Yet, only activated T cells display a functional form of PSGL-1 and bind avidly to P-selectin. Such activation-dependent binding activity appears to be a result of differential post-translational modification, as suggested by elevated levels of alpha (1,3) fucosyltransferases activities in activated T cells. PSGL-1 isoforais also show differential affinity to L-selectin and E-selectin. For instance, human T cells exhibiting the CLA-positive isoform can tether and roll on both E- and P-selectin, while T cells expressing PSGL-1 without the CLA epitope only bind to P-selectin. Furthermore, binding of PSGL-1 to P-selectin is contingent upon the presence of the terminal decapeptide that contains three tyrosine residues for sulfation and one threonine residue for glycosylation.

A PSGL-1 protein can be prepared by recombinant methods and/or by isolating a native PSGL-1 protein from biological material. A recombinant PSGL-1 protein can be produced in prokaryotic or eukaryotic cells, either in vitro or in vivo. Nucleic acids encoding PSGL-1 can be used for recombinant production of the protein (see, e.g., GenBank™ Accession NM_003006 for an example of a nucleic acid encoding a PSGL-1 polypeptide). Antibodies directed to PSGL-1 are also well known and can be used for purification of the antigen (see, e.g., Herron et al. (2000) Science Jun 2;28S(5471): 1653-56; WO 00/2580S) and/or used in methods described herein. PSGL-1 is further described in references including but not limited to Sako et al. (1993) Cell 75:1179; Vachino et al. (1995) J. Biol. Chem. 270:21966; and Veldman et al. (1995) J. Biol. Chem. 270:16470.

For recombinant production of PSGL-1, the simultaneous expression of both PSGL-1 and its modifying alpha (1,3) fucosyltransferase, Fuc-TVII, may be required for the functional expression of PSGL-1. In addition or alternatively, recombinant production of PSGL-1 may be accompanied by co-transfection with a nucleic acid encoding PACE for removing the propeptide and/or or a nucleic acid encoding tyrosine sulfotransferase.

WO 2005/027831 PCT/US2O04/02952I) An anti-PSGL-1 antibody can be used to isolate and purify a PSGL-1 antigen from biological material. Any cell type expressing a PSGL-1 protein, e.g., T cells derived from an individual or a T cell line, can be used as a source of the protein.

Once purified, the protein can be used in a variety of methods as described herein. For example, the purified PSGL-1 protein can be used in an in vitro screen of modulators of PSGL-1 function on T cells or as an immunogen to prepare antibodies directed against the protein.

Anti-PSGL-1.Antibodies PSGL-1 polypeptides (or immunogenic fragments or analogs thereof) can be used to generate antibodies useful in the methods of the invention. As described above, PSGL-1 polypeptides or peptide fragments thereof can be produced by recombinant techniques or synthesized using solid phase synthesis methods. The recombinant PSGL-1 polypeptides or a peptide fragment thereof can be used as an immunogen to produce anti-PSGL-1 antibodies. In addition, an anti-PSGL-1 antibody, such as the TAB4 monoclonal antibody, can be used to purify a PSGL-1 polypeptide, e.g., a PSGL-1 polypeptide in its natural conformation, which can then be used as an immunogen to produce additional anti-PSGL-1 antibodies.

An antibody of the invention can be a monoclonal, polyclonal, or engineered antibody that specifically binds to a PSGL-1 polypeptide. An antibody that "specifically binds" to a particular antigen, e.g., a PSGL-1 polypeptide, will not substantially recognize or bind to other molecules in a sample. Thus, the invention also features methods for identifying a test compound (e.g., an antibody) that binds to a polypeptide of the invention by contacting the polypeptide with a test compound and determining whether the polypeptide binds to the test compound (e.g., by direct detection of the binding, detection of a competitor molecule which disrupts binding of the test compound to the polypeptide, and/or detection of binding using an assay for apoptosis-inducing activity).

In general, PSGL-1 polypeptides can be coupled to a carrier protein, such as KLH, mixed with an adjuvant, and injected into a host mammal. Antibodies produced in that animal can then be purified by peptide antigen affinity chromatography.

In particular, various host animals can be immunized by injection with a PSGL-1 polypeptide or an antigenic fragment thereof. Commonly employed host animals include rabbits, mice, guinea pigs, and rats. Various adjuvants that can be used to increase the immunological response depend on the host species and include Freund's adjuvant (complete and incomplete), mineral gels such as aluminum hydroxide, surface active substances such as lysolecithin, pluronic polyols, polyanions, peptides, oil emulsions, keyhole limpet hemocyanin, and dinitrophenol. Potentially useful human adjuvants include BCG (bacille Calmette-Guerin) and Corynebacterium parvum. Polyclonal antibodies are heterogeneous populations of antibody molecules that are contained in the sera of the immunized animals.

Antibodies within the invention therefore include polyclonal antibodies and, in addition, monoclonal antibodies, humanized or chimeric antibodies, single chain antibodies, Fab fragments, F(ab')2 f agments, and molecules produced using a Fab expression library.

Monoclonal antibodies, which are homogeneous populations of antibodies to a particular antigen, can be prepared using the PSGL-1 polypeptides described above and standard hybridoma technology (see, for example, Kohler et al., Nature 256:495 [1975]; Kohler et al., Eur J Immunol 6:511 [1976]; Kohler et al., Eur J Immunol 6:292 [1976]; Hammerling et al., Monoclonal Antibodies and T Cell Hybridomas, Elsevier, N.Y. [1981]).

In particular, monoclonal antibodies can be obtained by any technique that provides for the production of antibody molecules by continuous cell lines in culture such as described in Kohler et al., Nature 256:495 (1975), and U.S. Pat. No. 4,376,110; the human B-cell hybridoma technique (Kosbor et al., Immunology Today 4:72 [1983]; Cole et al, Proc Natl Acad Sci USA 80:2026 [1983]), and the EBV-hybridoma technique (Cole et al, Monoclonal Antibodies and Cancer Therapy, Alan R. Liss, Inc., pp. 77-96 [19S3]). Such antibodies can be of any immunoglobulin class including IgG, IgM, IgE, IgA, IgD and any subclass thereof. The hybridoma producing the niAb of this invention may be cultivated in vitro or in vivo. The ability to produce high titers of mAbs in vivo makes this a particularly useful method of production.

Once produced, polyclonal or monoclonal antibodies are tested for specific PSGL-1 recognition by Western blot or immimoprecipitation analysis by standard methods, for example, as described in Ausubel et al, supra. Antibodies that specifically recognize and bind to PSGL-1 are useful in the invention. Anti-PSGL-1 antibodies that bind to the PSGL-1 antigen on the surface of a T cell, e.g., a CD3+ cell, and induce the depletion and/or apoptosis of T cells in an individual are particularly useful.

The antibodies can be used, for example, as part of a therapeutic regime (e.g., to reduce or eliminate an undesirable immune response, such . as a T cell mediated immune response, associated with conditions such as inflammatory diseases, autoimmune diseases, transplant rejection, allergic diseases, and T cell-derived cancers). Antibodies also can be used in a screening assay to measure the ability of a candidate compound to bind to PSGL-1.

In addition, techniques developed for the production of "chimeric antibodies" (Morrison et al., Proc Natl Acad Sci USA SI :6S51 [1984]; Neuberger et al., Nature 312:604 [1984]; Talceda et al., Nature 314:452 [1984]) by splicing the genes from a mouse antibody molecule of appropriate antigen specificity together with genes from a human antibody molecule of appropriate biological activity can be used. A chimeric antibody is a molecule in which different portions are derived from different animal species, such as those having a variable region derived from a murine monoclonal antibody and a human immunoglobulin constant region.

Alternatively, techniques described for the production of single chain antibodies (U.S. Pat. Nos. 4,946,77S, 4,946,77S, and 4,704,692) can be adapted to produce single chain antibodies against a PSGL-1 polypeptide, or a fragment thereof. Single chain antibodies are formed by linking the heavy and light chain fragments of the Fv region via an amino acid bridge, resulting in a single chain polypeptide.

Antibody fragments that recognize and bind to specific epitopes can be generated by known techniques. For example, such fragments include but are not limited to F(ab')2 fragments that can be produced by pepsin digestion of the antibody molecule, and Fab fragments that can be generated by reducing the disulfide bridges of F(ab')2 fragments. Alternatively, Fab expression libraries can be constructed (Huse et al, Science 246:1275 [1989]) to allow rapid and easy identification of monoclonal Fab fragments with the desired specificity.

Antibodies can be humanized by methods known in the art. For example, monoclonal antibodies with a desired binding specificity can be commercially humanized (Scotgene, Scotland; Oxford Molecular, Palo Alto, Calif.). Fully human antibodies, such as those expressed in transgenic animals are also features of the invention (Green et al., Nature Genetics 7:13 [1994]; and U.S. Pat. Nos. 5,545,806 and 5,569,825).

Multimeric Compounds.

Multimeric compounds that bind to a plurality of PSGL-1 proteins on the surface of a T cell or NIC cell can be used to induce apoptosis in the cell. The multimeric compound contains at least two polypeptide chains. Each of the polypeptide chains contains (i) a binding domain that binds to PSGL-1 , and (ii) a heterologous amino acid sequence.

In general, a multimeric compound binds to at least two different PSGL-1 proteins on the surface of a given cell. However, a multimeric compound can be formulated to have 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or more distinct PSGL-1 binding domains, thereby causing the multimeric compound to bind to 3, 4, 5, 6, 7, 8, 9, 10, 1 1, 12, or more different PSGL-1 proteins on the surface of a given cell.

A binding domain can contain any amino acid sequence (or any amino acid sequence with a modification such as, e.g., glycosylation and/or sulfation) that binds to PSGL-1. The binding domain can correspond to either a naturally occurring or a non-naturally occurring amino acid sequence. For example, a binding domain can contain the PSGL-1 -binding domain of a selectin (e.g., P-selectin, E-selectin, or L-selectin). A polypeptide containing a PSGL-1 binding domain of a selectin can optionally include: (i) an extracellular domain of the selectin (e.g., P-selectin, E-selectin, or L-selectin); (ii) a calcium dependent lectin domain of the selectin (e.g., P-selectin, E-selectin, or L-selectin); or (iii) a fragment of the extracellular domain of the selectin (e.g., P-selectin, E-selectin, or L-selectin) that mediates binding to PSGL- VVO 2005/027831 PCT/US2()()4/02, 52() 1. In addition to these naturally occurring amino acid sequences, one or more amino acid changes can be introduced into a naturally occivrring PSGL-1 binding domain, resulting in a non-naturally occurring sequence that retains PSGL-1 -binding function. For example, a polypeptide can contain an amino acid sequence that binds to PSGL-1 and is at least S0%, 85%, 90%, 95%, or 98% identical to any of: (i) an extracellular domain of a selectin (e.g., P-selectin, E-selectin, or L-selectin); (ii) a calcium dependent lectin domain of a selectin (e.g., P-selectin, E-selectin, or L-selectin); or (iii) a fragment of the extracellular domain of a selectin (e.g., P-selectin, E-selectin, or L-selectin) that mediates binding to PSGL-1. Standard molecular biology ' mutagenesis tecluiiques can be used to infaOduce changes into a nucleic acid sequence encoding a PSGL-1 binding domain. Modified binding domains can then be tested for their ability to bind to PSGL-1, e.g., immobilized PSGL-1 or PSGL-1 on the surface of a cell. A binding domain can also contain the PSGL-1 binding domain of an anti-PSGL-1 antibody or a polypeptide selected from a phage display library, or a an amino acid sequence that binds to PSGL-1 and is at least 80%, 85%, 90%, 95%, or 98% identical to the PSGL-1 binding domain of an anti-PSGL-1 antibody or a polypeptide selected from a phage display library.

A PSGL-1 -binding domain can contain an amino acid sequence that corresponds to a PSGL-1 -binding fragment of P-selectin. An example of a polypeptide chain (of a multimeric compound described herein) containing such an amino acid sequence is a recombinant mouse P-selectin/Fc chimera (available from R&D Systems, Mimieapolis, MN) containing the following components: (i) CD33 signal peptide (Metl-Alal6); (ii) mouse P-selectin (Trp42-Ala709 of extracellular domain); (iii) IEGRMD (SEQ ID NO:l); and (iv) Human IgGl (Prol00-Lys330). A second example of a polypeptide chain containing such an amino acid sequence is a recombinant human P-selectin/Fc chimera (available from R&D Systems, Minneapolis, MN) containing the following components: (i) human P-selectin (Metl- Ala771, extracellular domain); (ii) IEGRMD (SEQ ID NO: l); and (iii) Human IgGl (Prol00-Lys330).

A PSGL-1 -binding domain can contain an amino acid sequence that corresponds to a PSGL-1 -binding fragment of E-selectin. An example of a polypeptide chain (of a multimeric compound described herein) containing such an amino acid sequence is a recombinant mouse E-selectin/Fc chimera (available from R&D Systems, Minneapolis, MN) containing the following components: (i) mouse E-selectin (Metl-Pro557, extracellular- domain); (ii) IEGRMD (SEQ ID NO:l); (iii) Human IgGl (Prol00-Lys330); and (ϊν)ΉΗΗΗΗΗ (SEQ ID NO:2). A second example of a polypeptide chain containing such an amino acid sequence is a recombinant human E-selectin/Fc chimera (available from R&D Systems, Minneapolis, MN) containing the following components: (i) human E-selectin (Metl-Pro556, extracellular domain); (ii) IEGRMD (SEQ ID NO:2); (iii) human IgGl (Prol 00-Lys330); and (iv) HHHHHH (SEQ ID NO:2).

A PSGL-1 binding domain can contain an amino acid sequence that corresponds to a PS GL-1 -binding fragment of L-selectin. An example of a polypeptide chain (of a multimeric compound described herein) containing such an amino acid sequence is a recombinant mouse L-selectin/Fc chimera (available from R&D Systems, Minneapolis, MN) containing the following components: (i) mouse L-selectin (Metl-Asn332, extracellular domain); (ii) IEGRMD (SEQ ID NO:l); (iii) Human IgGl (Prol00-Lys330); and (iv) HHHHHH (SEQ ID NO:2). A second example of a polypeptide chain containing such an amino acid sequence is a recombinant human L-selectin/Fc chimera (available from R&D Systems, Minneapolis, MN) containing the following components: (i) human L-selectin (Metl-Asn332, extracellular domain); (ii) IEGRMD (SEQ ID NO:l); (iii) Human IgGl (Prol00-Lys330); and (iv) HHHHHH (SEQ ID NO:2).

A multimeric compound can be formulated as a homo-multimeric compound or a hetero-multimeric compound. A homo-multimeric compound contains only polypeptide chains that have identical PSGL-1 binding domains. For example, a homo-multimeric compound can contain polypeptide chains containing identical PS GL-1 -binding fragments of P-selectin. A hetero-multimeric compound contains polypeptide chains that have different PSGL-1 binding domains. For example, a hetero-multimeric compound can contain 'a first polypeptide chain that contains a PS GL-1 -binding fragment of P-selectin and a second polypeptide chain that contains a PS GL-1 -binding fragment of E-selectin. 19 WO 2005/1) 27831 PCT/U S2()()4/I)2 52() A heterologous amino acid sequence can be any amino acid sequence.

However, the amino acid sequence of the polypeptide chains described herein does not correspond to the sequence of a naturally occurring protein. A heterologous amino acid sequence contains one or more amino acids that permit the linkage of the polypeptide chains. For example, the one'or more amino acids can covalently link, e.g., via a disulfide linkage, the polypeptide chains. One example of a heterologous sequence is an immunoglobulin heavy chain constant region. Disulfide bonding between Fc regions of two polypeptide chains can result in the formation of a dimeric compound. hi addition to contributing to the linkage of the polypeptide chains, the heterologous amino acid sequence can also contain a cross-linker binding region, e.g., a cell surface receptor binding region. Upon the binding of an agent to such a binding region, cross-linking of the polypeptide chains and the cell surface PSGL-1 proteins to which they are bound can result. An immunoglobulin heavy chain constant region contains an Fc receptor binding region. A cross-linker can be, for example, an antibody (e.g., an anti-Fc antibody) that specifically binds to the cross-linker binding region of the heterologous amino acid sequence.

Screening Assays for Compounds that Modulate PSGL-1 Function The invention also encompasses methods for identifying compounds that interact with PSGL-1 (or a domain of PSGL-1) including, but not limited to, compounds that induce T cell depletion and/or T cell apoptosis upon binding to PSGL-1. Also included are compounds that modulate the interaction of PSGL-1 with transmembrane, extracellular, or intracellular proteins that regulate PSGL-1 activity and compounds which modulate PSGL-1 activity.

The compounds that may be screened in accordance with the invention include, but are not limited to peptides, antibodies and fragments thereof, and other organic compounds that bind to PSGL-1 and modulate a biological function mediated by PSGL-1, as described herein.

Such compounds may include, but are not limited to, peptides such as, for example, soluble peptides, including but not limited to members of random peptide libraries; (Lam et al., Nature 354:82 [1991]; Houghten et al., Nature 354:84 [1991]), and combinatorial chemistry-derived molecular library made of D- and/or L configuration amino acids, phosphopeptictes (including, but not limited to, members of random or partially degenerate, directed phosphopeptide libraries; Songyang et al., Cell 72:767 [1993]), antibodies (including, but not limited to, polyclonal, monoclonal, humanized, anti-idiotypic, chimeric or single chain antibodies, and FAb, F(ab')2 and FAb expression library fragments, and epitope-binding fragments thereof), and small organic or inorganic molecules.

Other compounds which can be screened in accordance with the invention include but are not limited to small organic molecules that affect an activity of the PSGL-1 protein, as described herein.

Computer modeling and searching technologies permit identification of compounds, or the improvement of already identified compounds, that can modulate PSGL-1 expression or activity. Having identified such a compound or composition, the active sites or regions are identified. Such active sites might typically be a binding site for a natural modulator of activity. The active site can be identified using methods known in the art including, for example, from the amino acid sequences of peptides, from the nucleotide sequences of nucleic acids, or from study of complexes of the relevant compound or composition with its natural ligand. hi the latter case, chemical or X-ray crystallographic methods can be used to find the active site by finding where on the factor the modulator (or ligand) is found.

Although described above with reference to design and generation of compounds which could alter binding, one could also screen libraries of known compounds, including natural products or synthetic chemicals, and biologically active materials, including proteins, for compounds which bind to a PSGL-1 protein and cause T cell depletion and/or induce T eel,! apoptosis.

In vitro systems may be designed to identify compounds capable of interacting with PSGL-1 (or a domain of PSGL-1). Compounds identified may be useful, for example, in modulating T cell activity as described herein and thus may be useful for the treatment of conditions associated with an excessive or unwanted T cell mediated immune response or excessive or unwanted T cell proliferation such as inflammation, autoimmunity, transplant rejection, an allergic condition, or a T cell cancer.

The principle of the assays used to identify compounds that bind to PSGL-1 involves preparing a reaction mixture of PSGL-1 (or a domain thereof) and the test compound under conditions and for a time sufficient to allow the two components to interact and bind, thus forming a complex which can be removed and/or detected in the reaction mixture. The PSGL-1 species used can vaiy depending upon the goal of the screening assay. In some situations it is preferable to employ a peptide corresponding to a domain of PS GL-1 fused to a heterologous protein or polypeptide that affords advantages in the assay system (e.g., labeling, isolation of the resulting complex, etc.) can be utilized.

The screening assays can be conducted in a variety of ways. For example, one method to conduct such an assay involves anchoring PSGL-1 protein, polypeptide, peptide or fusion protein or the test substance onto a solid phase and detecting PSGL-1 /test compound complexes anchored on the solid phase at the end of the reaction. In one embodiment of such a method, the PSGL-1 reactant may be anchored onto a solid surface, and the test compound, which is not anchored, may be labeled, either directly or indirectly. hi practice, microtiter plates may conveniently be utilized as the solid phase.

The anchored component may be immobilized by non-covalent or covalent attachments. Non-covalent attachment may be accomplished by simply coating the solid surface with a solution of the protein and drying. Alternatively, an immobilized antibody, preferably a monoclonal antibody, specific for the protein to be immobilized may be used to anchor the protein to the solid surface. The surfaces may be prepared in advance and stored.

In order to conduct the assay, the nonimniobilized component is added to the coated surface containing the anchored component. After the reaction is complete, unreacted components are removed (e.g., by washing) under conditions such that any complexes formed will remain immobilized on the solid surface. The detection of complexes anchored on the solid surface can be accomplished in a number of ways.

Where the previously non-immobilized component is pre-labeled, the detection of label immobilized on the surface indicates that complexes were formed. Where the previously non-immobilized component is not pre-labeled, an indirect label can be used to detect complexes anchored on the surface; e.g., using a labeled antibody specific for the previously non-immobilized component (the antibody, in turn, may be directly labeled or indirectly labeled with a labeled anti-Ig antibody).

Alternatively, a reaction can be conducted in a liquid phase, the reaction products separated from unreacted components, and complexes detected; e.g., using an immobilized antibody specific for PSGL-1 protein, polypeptide, peptide or fusion protein or the test compound to anchor any complexes formed in solution, and a labeled antibody specific for the other component" of the possible complex to detect anchored complexes.

Alternatively, cell-based assays can be used to identify compounds that interact with PSGL-1. To this end, cell lines that express PSGL-1, or cell lines that have been genetically engineered to express PSGL-1 can be used. Cell based assays are particularly useful for evaluating the functional effects of a compound identified by a screen described herein. For example, once a compound is identified based upon its ability to bind to a PSGL-1 protein, the compound can then be tested for its ability to, e.g., induce T cell apoptosis in vitro or in vivo or deplete T cells in vitro or in vivo.

Pharmaceutical Compositions Given that an object of the present invention is to alter an immune response in an individual, a pharmaceutical composition containing, for example, antibodies, multimeric compounds, small molecules, or other compounds that specifically bind PSGL-1 polypeptides are also a feature of the invention. In a preferred example, the compound functions as an agonist of PSGL-1.

Pharmaceutical compositions for use in accordance with the present invention can be formulated in a conventional manner using one or more physiologically acceptable carriers or excipients. Thus, the compounds and their physiologically 23 acceptable salts and solvates may be formulated for administration by a variety of routes of administration.

The compounds may be formulated for parenteral administration by injection, for example, by bolus injection or continuous infusion. Formulations for injection may be presented in unit dosage form, for example, in ampoules or in multi-dose containers, with an added preservative. The compositions may take such forms as suspensions, solutions, or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilizing and/or dispersing agents.

Alternatively, the active ingredient can be in powder form for constitution with a suitable vehicle, for example, sterile pyrogen-free water, before use.

Methods of Controlling a T Cell-Mediated Immune Response and Depleting T Cell Populations Compounds such as those detailed in the screening assays described herein may be useful, for example, in modulating a biological function mediated by a PSGL-1 polypeptide and/or for the treatment of disorders associated an excessive or unwanted immune response, e.g., a T cell-mediated immune response. These compounds include, but are not limited to peptides, antibodies and fragments thereof, and other organic compounds that bind to PSGL-1 on the surface of a T cell and induce a signal transduction pathway that results in the death of the T cell. The methods of the invention optionally include the addition of a cross-linlcing agent that induces the cross-linlcing of PSGL-1 on the surface of a cell. The compounds described herein can be used in any instance wherein the depletion or elimination of T cell activity is desired. Particularly useful conditions that can be treated with the compounds of the invention include inflammatory diseases, autoimmune diseases, transplant rejection, allergic diseases, and T cell-derived cancers.

Examples of conditions that can be treated with the anti-PSGL-1 compounds described herein mclude, but are not limite'd to, diabetes mellitus, artlmtis (including rheumatoid artlmtis, juvenile rheumatoid artlmtis, osteoarthritis, and psoriatic arthritis), multiple sclerosis, encephalomyelitis, myasthenia gravis, systemic lupus erythematosis, autoimmune thyroiditis, dermatitis (including atopic dermatitis and eczematous dermatitis), psoriasis, Sjogren's Syndrome, Crohn's disease, aphthous ulcer, iritis, conjunctivitis, keratoconjunctivitis, type I diabetes, mfiammatory bowel diseases, ulcerative colitis, asthma, allergic asthma, cutaneous lupus erythematosus, scleroderma, vaginitis, proctitis, drug eruptions, leprosy reversal reactions, erythema nodosum leprosum, autoimmune uveitis allergic encephalomyelitis, acute necrotizing hemorrhagic encephalopathy, idiopathic bilateral progressive sensorineural hearing loss, aplastic anemia, pure red cell anemia, idiopathic thrombocytopenia, polychondritis, Wegener's granulomatosis, clironic active hepatitis, Stevens-Jolmson syndrome, idiopathic sprue, lichen planus, Graves' disease, sarcoidosis, primary biliary cirrhosis, uveitis posterior, interstitial lung fibrosis, graft-versus-host disease, cases of transplantation (including transplantation using allogeneic or xenogeneic tissues) such as bone marrow transplantation, liver transplantation, or the transplantation of any organ or tissue, allergies such as atopic allergy, AIDS, and T-cell neoplasms such as leukemias and/or lymphomas.

The methods of the invention can be used to deplete T cells from a cell population, either in vitro or in vivo. For example, a biological sample derived from an individual can be depleted of T cells in vitro by contacting the sample with an anti-PSGL-1 compound described herein, optionally together with a cross-linldng agent. This method can be useful, e.g., by allowing for the enrichment of non-T cells in a cell population as well as by reducing or eliminating T cell activity from a cell population.

The following are examples of the practice of the invention. They are not to be construed as limiting the scope of the invention in any way.

WO 2005/027831 PCT/US2()04/()2!>520 Examples Example 1 : Preparation of an Anti-T Cell Apoptosis Inducing Protein C'TAIP") Monoclonal Antibody A TAJP-specific monoclonal antibody was generated by applying the well known cell fusion methods of Kohler and Milstein ((1976) European Journal of Immunology 6:511-519) to produce a hybridoma secreting desired antibodies.

Antibody-producing cells from a hamster injected with Concanovalin A (Con A)- activated Balb/c spleen T cells were fused with a myeloma cell line to form an antibody secreting hybridoma. The two populations of cells were fused with polyethylene glycol, and the resulting antibody producing cells were cloned and propagated by standard tissue culture methods. One hybridoma generated according to these methods secreted a monoclonal antibody, designated TAB4, that was able to induce T cell apoptosis in vitro and deplete T cells in vivo. The protein recognized by TAB4 was designated T cell apoptosis inducing protein (ΤΑΓΡ).

C57BL/6J (B6) and BALB/c mice were purchased from the Jackson lab (Bar Harbor, ME). Syrian hamsters were purchased from the Animal Core Facility, National Taiwan University Medical College.

Concentrated culture supernatant of the TAB4 hybridoma was spun at 20,000 x g for 10 minutes and the supernatant was diluted at a 1 :1 ratio with the binding buffer (0.1 M sodium acetate, pH 5.0). A protein G column (approximately 1 ml bed volume) was washed three times with 3-5 ml of the binding buffer. The cleared culture supernatant was loaded to the protein G column and the flow-through was collected and reloaded to the column. The column was washed with 6-10 ml of the binding buffer and the bound antibody was eluted from the column with 5 ml of the elution buffer (0.1 M glycine-HCl, pH 2.S). Each fraction contained 1 ml of the eluted antibody and the eluted fraction was adjusted to neutral pH by mixing each 1 ml fraction with 50 microliters of 1 M Tris'-HCl, pH 7.5. Fractions containing the antibody were pooled and dialyzed against 2 liters of PBS, pH 7.4 three times at three hours for each dialysis. Protein concentration in the antibody samples were 26 deteimined with the procedure described by Bradford using the Bio-Rad Protein Assay (BIO-RAD, Hercules, CA).

Example 2: Preparation of a Mouse Spleen Cell Suspension and the Activation and Enrichment of T cells Mouse spleen was immersed in 8 ml of Hank's balanced salt solution (HBSS), gently minced with a sterile cover slip, transferred to a 15 ml centrifuge tube (Costar), and spun at 200 x g for 5 minutes. The supernatant was discarded and the cell pellet was resuspended in the residual buffer by gently tapping the wall. The contaminating red blood cells (RBC) were lysed by the addition of 1 ml of RBC lysis buffer (0.6 M NH C1, 0.17 M Tris-base, pH 7.65), followed by a 2 min incubation at room temperature and rapid quenching with 9 ml of HBSS. The cells were pelleted at 200 x g for 5 minutes, washed twice and resuspended in RPMI medium. The concentration and viability of cells in the mixture Avere deteimined with a hemocytometer (Cambridge Scientific Inc.) and Trypan blue exclusion.

The spleen cells were adjusted to a final concentration of 3 X lOVml with RPMI medium and Concanovalin A was added to a final concentration of 2 micrograms/ml to activate the T cells. The cell suspension was transferred to a 6-well culture plate (5 ml/well) or a 10-cm culture, dish (10 ml/dish) and incubated at 37°C, 5% CO2 for 4S hours before harvesting. The activated spleen cells, including activated T cells, were resuspended in 5 ml of HBSS and carefully overlaid on top of a 5 ml 55% cushion of Percoll solution in a centrifuge tube. Care was taken not to disturb the separated layers. The cells were spun at 1,900 x g for 13 minutes at 25°C without the brake. The enriched T cells were collected from the interface of the two layers, washed twice with HBSS, and were ready for experiments.

Example 3: Apoptosis of Activated T cells Activated T cells (see Example 2) were resuspended to a final concentration of 5 X 105 cells/ml in RPMI medium containing 5 ng/ml of IL-2, and treated with control Ig, TAB4, or anti-CD3 according to the conditions shown in Table 1. 27 TABLE 1 After an incubation period of 18-24 hours; .the extent of apoptosis in each culture was determined using the 7-AAD apoptosis assay. The treated cells were transferred to FACS tubes (Falcon), washed twice with ice-cold FACS solution (1% fetal bovine serum, 0.05% sodium azide in PBS), pelleted at 200 x g at 4°C. The cells were resuspended in ice-cold FACS solution to a final concentration of 1-2 X 107 cells/ml. For staining, 0.1 ml of the resuspended cells were mixed with 7-AAD to a final concentration of 2 ug/ml and then incubated at 4°C in the dark for 20 minutes. Finally, the stained cells were washed twice with ice-cold FACS solution, resuspended in 0.5 ml of FACS solution and analyzed with BD LSR flow cytometer (Beckton Dickison).

Fig. 1 depicts the results of a representative time-course experiment that investigated when activated T cells acquire sensitivity to TAB4 (anti-TAIP)-mediated apoptotic signals. Mouse splenocytes were activated with Con-A and maintained in IL-2 containing medium. Activated T cells were harvested, resuspended, and challenged with TAB4 monoclonal antibody or control hamster IgG in the presence of anti-hamster IgG antibody as cross-linker. The ability of ΤΑΓΡ cross-linking to induce low level (6.5%). of apoptotic ceil death Avas evident on day one. However, the extent of TAB4-induced apoptosis increased from 17% on day 2, peaked at 52% on day 4, and declined to 44% on day 6. The* control hamster IgG did not induce specific apoptotic T cell death, as compared with the cultures that received only IL-2. Anti-CD3 (as positive control) induced apoptosis in 38% of T cell after 48 hours of activation (data not shown).

Example 4: Expression of the ΤΑΓΡ Antigen in Different Tissues Cells were washed twice with ice-cold FACS solution (1% fetal bovine serum, 0.05% sodium azide in PBS) and spun at 200 x g at 4°C in a FACS tube (Falcon). The cells were resuspended in ice-cold FACS solution to a final concentration of 1 x 107 cells/ml and a 0.1 ml aliquot of the resuspended cells in a FACS tube (Falcon) was used for each assay. For surface staining, the TAB4 monoclonal antibody or a control hamster Ig at a final concentration of 2 ug/ml were added to the cells and the mixtures were incubated at 4°C for 30 minutes in the dark. The cells were washed once with ice-cold FACS and then stained with: (1) for spleen cells, cychrome-conjugated anti-CD3 antibody (2 ug/ml), FITC-conjugated anti-hamster Ig, and PE-conjugated anti-CD8/CD4/CD19/CDl lb/pan-NK/I-A/I-E/Mac-3 antibody (2 ug/ml) in 100 ul of ice-cold FACS solution; and (2) for thymus cells, FITC-conjugated anti-hamster Ig, PE-conjugated anti-CD 8, and cychrome-conjugated anti-CD4 antibodies (2 ug/ml) in 100 ul of ice-cold FACS solution. The reaction was performed at 4°C for 30 minutes in the dark. Finally, the stained cells were washed twice with ice-cold FACS solution, resuspended in 1 ml of FACS solution and analyzed with BD LSR flow cytometer (Beckton Dickison).

Figs. 3 and 4 demonstrate by FACS analysis the distribution of ΤΑΓΡ antigen on the various subpopulations of splenocytes and thymocytes. As shown in Fig. 3, CD19+ B cells expressed low but detectable amounts of ΤΑΓΡ proteins on the surface. Significantly higher amounts of ΤΑΓΡ proteins were detected on CD3+ T cells and a fi-action of NIC cells. Most of the CD4+, CD8+, and CD4+8+ thymus T cells expressed significant amounts of ΤΑΓΡ proteins. In contrast, the ΤΑΓΡ proteins were expressed only on a small population of CD4"8" thymus T cells (Fig. 4).

Tissues from B6 and BALB/c mice, including brain, thymus, heart, lung, liver, stomach, kidney, spleen, and skin, were collected, fixed in 10% formaldehyde overnight at room temperature, and embedded in paraffin blocks. Tissue sections, at a 4 urn thickness, were prepared from the paraffin block with Leica RM2135 microtome, spread in 45°C water, and laid on a coated slide. The slides were dried in 37°C and were ready for subsequent experiments. 29 WO 2005/(127831 PCT/US2IH)4/(I2 52(I Slides containing the tissue paraffin sections were dewaxed and dried through a xylenes-100% ethanol series according to standard protocol and were finally kept in 100% ethanol. The sections were rehydrated through a 100% ethanol-90% ethanol-85% ethanol-70% ethanol-PBS serial incubation according to standard protocol to a final PBS solution. The following reactions were all performed in a humidified box. Non-specific binding were blocked by incubating the tissue sections in blocking buffer (1% normal goat serum) for 1 hour at room temperature (or 4°C overnight). The blocking buffer was removed and TAB4 or normal hamster Ig (1 :200 dilution) was added to the sections and incubation continued for another hour at room temperature (oM°C overnight). The sections were washed twice in PBS, for 5 minutes each, to remove the primary antibody, reacted with 1 :250 diluted alkaline phosphatase-conjugated goat anti-hamster Ig, and incubated at room temperature for 1 hour. The sections were again washed twice with PBS, 5 minutes each, to remove the antibody-enzyme conjugate and the color reaction was developed with BCIP NBT substrate solution at room temperature for 30 minutes in the dark. The sections were washed again with PBS to remove excess enzyme substrate, dehydrated through the PBS-ethanol-xylenes series, and mounted for microscopy.

The results indicated that the ΤΑΓΡ proteins expression were detected only in bone marrow derived-tissues but not on the rest of the tissues tested.

Example 5: Cell Surface Biotinylation and Immunoprecipitation of the ΤΑΓΡ Antigen x 107 RL^l or ΝΓΗ-3Τ3 cells were surface biotinylated in 1 ml of PBS containing 0.5 mg/ml Sulfo-NHS-biotin (Pierce) for 30 minutes on ice. The reaction was terminated by incubating the cells with 0.5 ml of Dulbecco's modified Eagle's medium (Life Teclmologies, Inc.) for 10 minutes on ice. Cells were washed with 1 ml of Dulbecco's modified'Eagle's medium once and with 1 ml of phosphate-buffered saline twice.

Labeled cells were lysed at a density of 5.0 x 107 cells/ml in cold lysis buffer (1% Triton X-100, 20 mM Tris-HCl, pH S.0, 160 mM NaCl, 1 mM CaCl2) containing complete protease inhibitor cocktail (Roche) for 15 minutes, and insoluble material was pelleted at 10,000 x g for 10 minutes; these and all subsequent steps were ■ performed at 4°C. For iinmunoprecipitation, the lysate was preincubated for minutes with 50 μΐ of packed protein G-Sepharose (Amersham Phannacia Biotech) to remove non-specifically binding proteins. Beads were pelleted, and aliquots of the supernatant (routinely corresponding to 5.0 ^ 107 cells) were incubated with 20 μΐ of protein G-Sepharose preloaded with 10 μ-g of mAb TAB4 or IgG from normal hamster serum. After incubation for 4 h at 4 °C, the resin was washed four times with washing buffer (0.05% Triton X-100, 50 niM Tris-HCl, pH S.5, 400 inM NaCL 1 mM CaCl2) 1 mg/ml ovalbumin), twice with a similar washing buffer, containing 250 mM instead of 400 mM NaCl. Proteins specifically bound to the TAB4 were eluted with 50 μΐ of 1 xSDS sample buffer. Eluted proteins were separated by 8 % SDS-PAGE and transferred to nitrocellulose membrane (Millipore). Filters were analyzed for biotinylated proteins with peroxidase-conjugated Avi din (PharMingen) and developed with the Chemiluminescence reagent (NEN™ Life Science Products).

As shown in Fig. 2, a biotinylated surface protein with a molecular weight of approximately 120-lc was identified by TAB4 in RL.1 cells (ΤΑΓΡ+ T cells), but not in 3T3 cells (TAD?" cells). In contrast, protein G sepharose coated with hamster normal serum could not retrieve this 120-kDa protein. These results suggest that this 120-kDa protein is the antigen recognized by monoclonal antibody TAB4 on the cell surface of T cells.

Example 6: Depletion of T Cells in vivo To examine the effects of TAB4 on populations of T cells and other cells in vivo, mice were injected with 300 ug of TAB4 or control hamster Ig intraperitoneally and, on day 4, splenocytes, thymocytes, and peripheral blood mononuclear cells were harvested for the total cell count and for the analyses of cell surface markers by FACS.

For FACS assays, the cells were fixed with 2% paraformaldehyde at 4°C for 20 minutes, washed twice, and resuspendbd in ice-cold FACS solution to a final concentration of 1 x 107 cells/ml. A 100 ul aliquot of the resuspended cells in a FACS tube (Falcon) was used for each assay. TAB4 or control hamster Ig at a final 31 concentration of 2 ug/ml were added to the cells and the mixtures were incubated at 4°C for 30 minutes in the dark. The cells were washed once with ice-cold FACS and reacted with: (1) for spleen cells, cychrome-conjugated anti-CD3 antibody (2 ug/ml), FITC-conjugated anti-hamster Ig and PE-conjugated anti-CDS/CD4/CD19/CDnb/paii-M Four days after the injection, the percentages of CD3+ T cells in peripheral blood leukocytes (PBL) decreased from 36.7% in control mice to 4.1% in TAB4-treated mice (Table 2). TAB4 treatment caused a slight reduction in the total number of splenocytes. However, in TAB4 treated mice, there was a 62% decrease in the number of CD3+ T cells, a 50% decrease in the number of K cells, and a slightly increase in the total number of CD 19+ B cells. Tire total number of thymocytes recovered from TAB4 treated mice was only 48% of the level seen in control (52% reduced). Moreover, except for CD4+ T cells, all other CDS+, CD4+CD8+, and CD4-CDS- T cells were reduced, with CD4+CD8+ subpopulation being the most profoundly affected (64.7% reduction). 32 TABLE 2 (representative data from three experiments) Example 7: Anti-ΤΑΓΡ Antibody does not Induce IL-2 or TNF-alpha Secretion Balb/c mice (H-2d) were intraperitoneally injected with 300 micrograms of TAB4 or control hamster Ig. Splenocytes were isolated 7 days after injection, and used as responders in culture with mitomycin C-treated C3H (H-2k) splenocytes (as stimulators). Three days later, the culture supematants were harvested and the IL-2 content was measured by ELISA set (PharMingen). As shown in Fig. 5, the IL-2 production was suppressed in responder cells derived from TAB4-treated mice as compared with that of control mice. The p'lasma levels of IL-2 and TNF-alpha were also analyzed and no significant difference was noted in the levels of IL-2 (or TNF-alpha) in the sera of the control and the TAB4 treated mice. Since production of IL-2 33 YVO 2005/027831 PCT/US2IMU/U29520 is central to the activity of T cells, the results show that a TAIP-specific antibody, such as TAB4, can be used in vivo to manipulate T cells and control unwanted T cell-mediated immune responses such. as those associated with autoimmune diseases and transplantation rejection.

Example S: Use of an Anti-TAIP Antibody to Prevent Transplant Rejection Mice (obtained from Jackson Laboratory) at 8 to 12 Aveeks of age were anesthetized with Acepromazin maleate (Fermenta Animal Health Co., Kansas City, MO). Prior to. skin grafting, non-thymectomized recipient C57BL/6 mice (H-2b) were injected intraperitoneally with 500 ug of TAB4 or isotype control antibodies seven days before skin transplant surgery. Seven days later, a lateral flank of skin from fully allogeneic mismatched Balb/cj mice (H-2d) was grafted on the lateral flank of the antibody pre-treated C57BL/6 mice. Seven days post transplantation, the mice were again injected with 500 ug of TAB4 or isotype control antibody. The mice were monitored every day after graft transplantation. The grafts were considered rejected when 50% donor skin was necrotic. The percent of graft survival is shown in Fig. 7 (n=8). The data show that TAB4 antibody treatments prolonged the survival of the allogeneic skin grafts.

Example 9: Identification of ΤΑΓΡ as PSGL-1 P-selectin glycoprotein ligand-1 (PSGL-1), also named CD162, is the main P-selectin ligand expressed on leukocytes, including T cells (Sako et al. (1993) Cell 75 :1179; Vachino et al. (1995) J. Biol. Chem. 270:21966; Veldman et al. (1995) J. Biol. Chem. 270:16470). Biochemical characteristics of ΤΑΓΡ, such as its molecular weight and its tendency for dimerization suggested the possibility that TAB4 may be analogous to PSGL-1. To investigate the relationship between these two antigens, the following were tested: 1) whether the antigen precipitated by TAB4 can be recognized by a commercially-available anti-PSGLl antibody; and 2) whether an anti-PSGLl antibody can deplete TAB4 from the cell lysate. 34 WO 201)5/027831 PCT/US2()()4/()2';520 RL 1 T cells were lysed at a density of 1.0 x 10s cells/ml in lysis buffer (1% Triton X-100, 20 niM Tris-HCL pH 8.0, 160 mM NaCl, 1 mM CaCl2) containing complete protease inhibitor cocktail for 1 hour, and insoluble material was pelleted at 10,000 g for 10 minutes. These and all subsequent steps were performed at 4°C. The lysate corresponding to 5.0 x 107 cells was incubated with 20 ul of protein G-Sepharose preloaded with 10 ug of anti-PSGL-1 mAb (clone 2PH1, PharMingen, San Diego, CA), anti-TAD? mAb, TAB4, or IgG from normal hamster serum. After incubation for 4 hours at 4°C, the beads were washed five times with washing buffer (0.05% Triton X-100, 50 M Tris-HCl,pH 8.5, 400 mM NaCl, 1 mM CaCl2, 1 mg/ml ovalbumin), and twice with a similar washing buffer, containing 250 mM instead of 400 mM NaCl. Bound proteins were eluted with'40 ul of lxSDS sample buffer.

Eluted proteins were separated by 6 % SDS-PAGE and transferred to a nitrocellulose membrane. The membranes were immunoblotted with anti-PSGL-1 mAb, and revealed by peroxidase-conjugated goat anti-rat IgG (H+L) followed by chemiluminescence (Renaissance, NEN) .

Surface biotinylated RL<3T T cells were lysed at a density of 1.0 x 10s cells/ml in lysis buffer. The cell extract was incubated with 20 ug of antibody bound to 40 ul of protein G-Sepharose at 4°C overnight. Depletions were done with anti-PSGL-1 mAb (2PH1) or control rat IgG, with TAB4 or control normal hamster serum.

Depleted lysates were further subjected to do immunoprecipitation with TAB4 or anti-PSGL-1 mAb, respectively, hiimunoprecipitates were separated on 6% SDS-polyacrylamide gel and detected by fluorography. As shown in Fig. 6, anti-PSGL-1 antibody can deplete ΤΑΓΡ protein from T cell lysates. hi addition, proteins immunoprecipitated with anti-ΤΑΓΡ antibody (TAB4) can be recognized by anti-PSGL-1 antibody by western analysis.

Example 10: Induction of Apoptosis in Human T Cells by an anti-PSGL-1 Antibody To determine the role played by PSGL-1 in the apoptosis of human T cells, time-course experiments were carried out to investigate when activated human T cells acquire sensitivity toward PSGL-l-mediated apoptotic signals. Human T cells were stimulated with phytohemagglutinin (PHA) mitogen and further expanded in IL-2- 35 containing medium. Activated T cells were harvested and then challenged with anti-PSGL-1 in the presence of IL-2 and cross-linking antibodies.

Human peripheral blood was taken from healthy adults, heprinized, and enriched for peripheral blood mononuclear cells (PBMC) based on differential density using Ficoll-Paque Plus (Pharmacia Biotech). The PBMC were activated with 1% PHA (Life Technologies, GibcoBRL) for 48 hours and subsequently maintained in recombinant human IL-2 (5 ng ml) through the assay period. To assess the apoptosis-inducing ability an anti-human PSGL-1 antibody, the activated cells were treated with: (1) 1 ug /ml of the anti-PSGL-1 antibody clone KPL-1 (BD PharMingen) plus cross-linker rabbit anti-mouse Ig (0.5 ug /ml) (Jackson ImmunoResearch Laboratories); (2) isotype control purified mouse Ig plus cross-linker rabbit anti-mouse Ig; or (3) cross-linker rabbit anti-mouse Ig alone. After six hours of treatment, the percentage of early apoptotic cells was determined by FACS, staining with anti-Annexin V (BD PharMingen) and PI (Sigma).

As shown in Fig. S, signaling triggered by PSGL-1 using an anti-PSGL-1 antibody plus the crosslinlcer tiggered significant level of apoptosis in PHA-activated human PBMC (mainly T cells). The percentage of apoptotic cells increased from 8.5% on days 3 to 24% on day 8 in anti-PSGLl treated cultures. Neither isotopic-matched control, nor the cross-linking antibodies alone, had any effect on these cells.

Example 11 : Use of anti-PSGL-1 Agonist Antibody to Treat Autoimmune Disease Non-obese diabetic (NOD) mice, a well-accepted autoimmune diabetes animal, were bred under standard conditions. Spontaneous diabetes developed in the NOD mice at the age of about 20 weeks. Li the experimental group, the mice received three doses of anti-PSGL-1 antibody (TAB4) intraperitoneally at 300 μg per mouse at age of 14, 15 aiid 17 weeks. Two additional injections with the same dose were given at the ages of 24 and 26 weeks. The control group was given hamster Ig at the same dose. Mice were monitored for glucose uria by Medi-Test Glucose strips (Macherey-Nagel, Germany) twice every week after the age of 15 weeks. Non-fasting urine glucose levels over 300 mg/dl for two consecutive measurements were considered diabetic. 36 As shown in Fig. 9, TAB4 (anti-PSGL-1) antibody treatment yielded significant protection as compared with control antibody treatment. Thus an anti-PSGL-1 antibody treatment can dampen the activity of autoimmune T cells and delay the onset of type I-diabetes.

Example 12: Binding of P-Selectin, E-Selectin. and L-Selectin to Activated T Cells To determine ability of selectins (P-Selectin, E-Selectin, and L-Selectin) to bind to activated T cells, freshly prepared splenocytes from C57BL/6 mice were activated and harvested at days 2, 4, and 6. Non-activated T cells (i.e., freshly prepared splenocytes at day 0) were also analyzed. The day 2 sample constituted 3 x 106 cells/ml of splenocytes that were activated with 2ug/ml of Concanavalin A (Con A) in DMEM + 10% FBS for 2 days. Live cells were isolated by Ficoll gradient separation. The day 4 sample was obtained from cells that were activated with Con A for 3 days and maintained in medium containing 5ng/ml of IL-2 for an additional day. The day 6 sample was derived from cells that were activated with Con A for 3 days and maintained in 5ng/ml of IL-2 for 3 days.

To assay the samples by FACS analysis, 2 x 105 cells per well from days 0, 2, 4 and 6 were incubated at 4°C for 30 minutes with 40 ul well of mouse P-Selectin, E-Selectin, or L-Selectin fused to the Fc region of human IgGl (R&D Systems, Minneapolis, MN) at concentrations ranging from 20 ug/inl with two-fold serial dilution to 0.156 ug/ml. Following the incubation, cells were washed with lx FACScan buffer (1 x PBS without calcium and magnesium ions from Biochrom AG, Berlin and 2% FBS). Samples were further incubated at 4°C for 30minutes with 95 ul/well of anti-Thyl.2 and a secondary reagent (FITC-anti human IgG, which is specific to Fc fragment, purchased from Jackson IrniiiunoResearch Laboratories, Inc., West Grove, PA) at 3.25 ug/ml, and then washed with lx FACScan buffer.

The results of FACSCalibur analysis are shown in Fig. 10. At 20 ug/ml, binding of P-selectin to mouse activated -cells increased gradually, peaked on day 4, and declined slightly on day 6. Binding of E-selectin rose significantly from day 2 to day 4 and then remained peaked at day 6. Binding of L-selectin to mouse activated T cells was not apparent, and did not change through the activation period, i.e. from day 37 0 to day 6. The results observed with L-Selectin could be due to the apparent low binding affinity of L-selectin to its ligand. Similar results were also obtained when lower concentrations of the three selectins were employed in the experiments.

Example 13: Multimeric Forms of E-Selectin and P-Selectin Induce Apoptosis of Activated T Cells A 96-well plate (NUNC) was coated with 50 ul of anti-human Fc Ig at 20 ug/ml in 1 x PBS at 4°C overnight, blocked with 1% BSA at 37°C for 2 hours and incubated with 50 ul of a selectin-human Fc fusion (from 0.063 to 5 ug/ml) at room temperature for 2 hours. In all experimental steps; each well was thoroughly washed five times with 1 x PBS. Then 2 x 105 T cells activated previously with Con A for four days were added into each well and incubated at 37°C for 5 hours prior to centrifugation of the plate at 200 x g for 5 minutes at 4°C. The resulting pellet containing activated T cells was incubated with Annexin V-biotin conjugate at room temperature for 15 minutes and subsequently with an avidin conjugate (SA-beta-gal at 1 :5000 dilution) for another 30 minutes at 37°C. In every binding reaction, each well was washed thrice with Annexin V binding buffer. The color development was achieved by incubating both 110 ul of Z-buffer mixture (54 ul of 2-mercaptoethanol in 20ml of Z-buffer) and 30 ul of ONPG (0.04g/10ml) at 4°C overnight. The readings of optical density at 420 nm were recorded.

Levels of selectin-induced apoptosis of Con-A activated T cells increased with the increasing concentrations (from 0.063 ug/ml to 5 ug/ml) of P-selectin (Fig. 11 A) or E-selectin (Fig. 1 IB) fused with Fc of human IgGl . The hamster antibody TAB4 induces apoptosis of activated T cells (see Example 1) and was used as a positive control in these experiments. As negative controls, anti-human Fc, human Ig (HIg), and BSA did not induce apoptosis. No significant apoptosis was detected in the presence of the L-selectin human Fc fusion protein (Fig. 11C), consistent with the failure of L-selectin to bind well to activated T cells (Example 12).

In summary, a plate-bound fusion protein containing a PS GL-1 -binding fragment of P-selectin or E-selectin and human Fc fragment induced apoptosis of activated T cells.

Example 14: Cross-Linking of Soluble P-Selectin-Fc Fusion Protein Induces Apoptosis of Activated T Cells · Mouse selectins (P-Selectin, E-Selectin, and L-Selectin) were fused to the Fc region of human IgGl as detailed above to form soluble dimeric fusion proteins. To evaluate whether the soluble selectins can induce apoptosis of activated T cells, an experiment was performed as detailed in the in Example 13, with the exception that the plate-bound anti-human Fc Ig was omitted. Negligible or low levels of apoptosis of activated T cells occurred in the presence of the soluble fonn of P-selectin fusion protein (a dimer) alone (Fig. 12). However, upon the addition of a cross-linker (anti-human Fc) the apoptotic activity increased substantially, to approximately the apoptotic level induced in the presence of the plate-bound antibody. Neither anti-human Fc, human Ig (HIg), nor BSA induced apoptosis.

Similar results were obtained for the E-selectin-Fc fusion protein as were obtained for the P-selectin-Fc fusion protein. In addition, consistent with the results obtained for the plate-bound (multimeric form) of L-selectin, the soluble form of L-selectin fusion protein did not induce apoptosis of activated T cells.

Other Embodiments It is to be understood that, while the invention has been described in conjunction with the detailed description thereof, the foregoing description is intended to illustrate and not limit the scope of the invention. Other aspects, advantages, and modifications of the invention are within the scope of the claims set forth below. 39

Claims (35)

174172/3 What is claimed is:

1. Use of a multimeric compound that binds to at least two PSGL-1 proteins on the surface of a T cell or natural killer (NK) cell in the manufacture of a preparation for inducing death of the T cell or the NK cell, wherein the multimeric compound (a) comprises two polypeptide chains, each of the polypeptide chains comprising (i) a binding domain that binds to PSGL-1, and (ii) a heterologous amino acid sequence, wherein the polypeptide chains are linked via the heterologous amino acid sequence to form the multimeric compound, and (b) does not include an anti-PSGL-1 antibody or an antibody fragment that binds to PSGL-1, wherein binding of the multimeric compound to the at least two PSGL-1 proteins on the surface of the T cell or NK cell induces a signal transduction pathway that results in death ofthe T cell or NK cell.

2. The use according to claim 1, wherein the multimeric compound is a homomultimeric compound.

3. The use according to claim 1, wherein the multimeric compound is a heteromultimeric compound.

4. The use according to claim 1, wherein the heterologous amino acid sequence comprises a cell surface receptor binding region.

5. The use according to claim 1, wherein the binding domain comprises a P-Selectin extracellular domain or a PSGL-1 -binding fragment thereof.

6. The use according to claim 1, wherein the binding domain comprises an E-Selectin extracellular domain or a PSGL-1 -binding fragment thereof.

7. The use according to claim 1, wherein the binding domain comprises an L-Selectin extracellular domain or a PSGL-1 -binding fragment thereof.

8. The use according to claim 1, wherein the binding domain comprises a 01658608\28-02 40 174172/4 PSGL-1 binding polypeptide selected from a phage display library.

9. The use according to claim 1, wherein the polypeptide chains are covalently linked via the heterologous amino acid sequence to form the multimeric compound.

10. The use according to claim 9, wherein the covalent linkage is a disulfide linkage.

11. The use according to claim 1, wherein the heterologous amino acid sequence comprises an immunoglobulin heavy chain constant region.

12. The use according to claim 1, wherein the binding of the multimeric compound to the at least two PSGL-1 proteins to induce the signal transduction pathway that results in the death of the T cell or the NK cell further comprises contacting the multimeric compound with an agent that binds to the multimeric compound via the heterologous amino acid sequence to induce cross-linking of a plurality of PSGL-1 antigens on the surface of the T cell or the NK cell.

13. The use according to claim 1, wherein the T cell is an activated T cell.

14. The use according to any one of Claims 1-13, wherein the cell is a T cell and wherein the medicament is suitable for preventing or treating a condition selected from an inflammatory disease, an autoimmune disease, transplant rejection an allergic condition and a T-cell cancer.

15. The use according to claim 14, wherein the condition is an inflammatory disease.

16. The use according to claim 14, wherein the condition is an autoimmune disease.

17. The use according to claim 16, wherein the autoimmune disease is rheumatoid arthritis.

18. The use according to claim 16, wherein the autoimmune disease is psoriasis.

19. The use according to claim 16, wherein the autoimmune disease is autoimmune diabetes.

20. The use according to claim 14, wherein the condition is transplant rejection.

21. The use according to claim 14, wherein the condition is an allergic disease. 41 174172/6

22. The use according to claim 14, wherein the condition is a T cell cancer.

23. A kit comprising: a multimeric compound that binds to at least two PSGL-1 proteins on the surface of a T cell or natural killer (NK) cell, wherein the multimeric compound (a) comprises two polypeptide chains, each of the polypeptide chains comprising (i) a binding domain that binds to PSGL-1, and (ii) a heterologous amino acid sequence, wherein the polypeptide chains are linked via the heterologous amino acid sequence to form the multimeric compound, and (b) does not include an anti-PSGL-1 antibody or an antibody fragment that binds to PSGL-1, wherein binding of the multimeric compound to the at least two PSGL-1 proteins on the surface of the T cell or NK cell induces a signal transduction pathway that results in death of the T cell or NK cell; and instructions for use of the multimeric compound to treat a condition selected from inflammation, autoimmunity, transplant rejection, an allergic condition, and a T cell cancer.

24. A multimeric compound that binds to at least two PSGL-1 proteins on the surface of a T cell or NK cell, wherein the multimeric compound comprises two polypeptide chains, each of the polypeptide chains comprising (i) a binding domain that binds to PSGL-1, and (ii) a heterologous amino acid sequence, wherein the polypeptide chains are linked via the heterologous amino acid sequence to form the multimeric compound, for use in inducing the death of a T cell or a natural killer (NK) cell, wherein the binding of the multimeric compound to the at least two PSGL-1 proteins on the surface of the T cell or NK cell induces a signal transduction pathway that results in the death of the T cell or NK cell, and wherein said compound is not an anti-PSGL-1 antibody or an antibody fragment that binds to PSGL-1.

25. The multimeric compound according to claim 24, is a homomultimeric compound.

26. The multimeric compound according to claim 24, is a heteromultimeric compound. 42 174172/3

27. The multimeric compound according to claim 24, wherein the heterologous amino acid sequence comprises a cell surface receptor binding region.

28. The multimeric compound according to claim 24, wherein the binding domain comprises a P-Selectin extracellular domain or a PSGL-1 -binding fragment thereof.

29. The multimeric compound according to claim 24, wherein the binding domain comprises an E-Selectin extracellular domain or a PSGL-1 -binding fragment thereof.

30. The multimeric compound according to claim 24, wherein the binding domain comprises an L-Selectin extracellular domain or a PSGL-1 -binding fragment thereof.

31. The multimeric compound according to claim 24, wherein the binding domain comprises a PSGL-1 binding polypeptide selected from a phage display library.

32. The multimeric compound according to claim 24, wherein the polypeptide chains are covalently linked via the heterologous amino acid sequence to form the multimeric compound.

33. The multimeric compound according to claim 32, wherein the covalent linkage is a disulfide linkage.

34. The multimeric compound according to claim 24, wherein the heterologous amino acid sequence comprises an immunoglobulin heavy chain constant region.

35. The multimeric compound according to any one of claims 24 to 34 for use in treating a condition selected from inflammation, autoimmumty, transplant rejection, an allergic condition, and a T cell cancer. For the Applicants RTNERS 43