IL143637A0 - Method for detecting in vitro a target substance in a sample comprising the labelling of said substance with a reporter gene and the sequences required for expressing said reporter gene in vitro - Google Patents
Method for detecting in vitro a target substance in a sample comprising the labelling of said substance with a reporter gene and the sequences required for expressing said reporter gene in vitroInfo
- Publication number
- IL143637A0 IL143637A0 IL14363799A IL14363799A IL143637A0 IL 143637 A0 IL143637 A0 IL 143637A0 IL 14363799 A IL14363799 A IL 14363799A IL 14363799 A IL14363799 A IL 14363799A IL 143637 A0 IL143637 A0 IL 143637A0
- Authority
- IL
- Israel
- Prior art keywords
- reporter gene
- vitro
- substance
- detecting
- expressing
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention concerns a method for detecting a target substance in a sample, characterised in that it comprises the following steps: (a) specifically labelling said substance with a reporter gene and with the sequences required for expressing said reporter gene in vitro; (b) in vitro transcription and translation of said reporter gene; (c) detecting in vitro the reporter protein coded by said reporter gene. The invention also concerns reagents and kits for implementing said method.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9815489A FR2786789B1 (en) | 1998-12-08 | 1998-12-08 | METHOD FOR IN VITRO DETECTION OF A TARGET NUCLEIC ACID SEQUENCE IN A NUCLEIC ACID SAMPLE |
PCT/FR1999/003061 WO2000034513A1 (en) | 1998-12-08 | 1999-12-08 | METHOD FOR DETECTING IN VITRO A TARGET SUBSTANCE IN A SAMPLE COMPRISING THE LABELLING OF SAID SUBSTANCE WITH A REPORTER GENE AND THE SEQUENCES REQUIRED FOR EXPRESSING SAID REPORTER GENE $i(IN VITRO) |
Publications (1)
Publication Number | Publication Date |
---|---|
IL143637A0 true IL143637A0 (en) | 2002-04-21 |
Family
ID=9533726
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IL14363799A IL143637A0 (en) | 1998-12-08 | 1999-12-08 | Method for detecting in vitro a target substance in a sample comprising the labelling of said substance with a reporter gene and the sequences required for expressing said reporter gene in vitro |
IL143637A IL143637A (en) | 1998-12-08 | 2001-06-07 | Method for detecting in vitro a target substance in a sample comprising the labelling of said substance with a reporter gene and the sequences required for expressing said reporter gene in vitro |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IL143637A IL143637A (en) | 1998-12-08 | 2001-06-07 | Method for detecting in vitro a target substance in a sample comprising the labelling of said substance with a reporter gene and the sequences required for expressing said reporter gene in vitro |
Country Status (10)
Country | Link |
---|---|
EP (1) | EP1137804B9 (en) |
JP (1) | JP4580104B2 (en) |
AT (1) | ATE259884T1 (en) |
AU (1) | AU772357B2 (en) |
CA (1) | CA2354986A1 (en) |
DE (1) | DE69914942T2 (en) |
ES (1) | ES2216598T3 (en) |
FR (1) | FR2786789B1 (en) |
IL (2) | IL143637A0 (en) |
WO (1) | WO2000034513A1 (en) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1531183A1 (en) * | 2003-11-14 | 2005-05-18 | bioMérieux BV | Method for amplification of RNA sequences |
US10808278B2 (en) | 2015-03-03 | 2020-10-20 | University Of Miami | Kits and methods for pathogen detection |
CA3149497A1 (en) | 2019-08-09 | 2021-02-18 | Nutcracker Therapeutics, Inc. | Methods and apparatuses for manufacturing for removing material from a therapeutic composition |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU1322692A (en) * | 1990-11-05 | 1992-05-26 | United States Of America, As Represented By The Secretary Of The Army, The | Method for the in vitro production of protein from a dna sequence without cloning |
US5235039A (en) * | 1991-06-10 | 1993-08-10 | Eli Lilly And Company | Substrates for hiv protease |
US6180338B1 (en) * | 1992-08-04 | 2001-01-30 | Beckman Coulter, Inc. | Method, reagent and kit for the detection and amplification of nucleic acid sequences |
WO1994005812A1 (en) * | 1992-09-02 | 1994-03-17 | The Scripps Research Institute | Coupled isothermal polynucleotide amplification and translation system |
AU4930993A (en) * | 1992-09-24 | 1994-04-12 | Public Health Research Institute Of The City Of New York, Inc., The | Coupled replication-translation methods and kits for protein synthesis |
US5436131A (en) * | 1993-04-02 | 1995-07-25 | Merck & Co., Inc. | Color screening assay for identifying inhibitor resistant HIV protease mutants |
EP0693131A4 (en) * | 1993-04-08 | 1998-06-10 | Res Dev Foundation | Cell free system for protein synthesis and use of chaperone proteins therein |
FR2721945B1 (en) * | 1994-07-04 | 1996-10-18 | David Fabrice | GENE ENHANCEMENT, A METHOD OF ISOTHERMAL GENE AMPLIFICATION AND ITS APPLICATIONS |
US6063562A (en) * | 1994-09-16 | 2000-05-16 | Sepracor, Inc. | In vitro method for predicting the evolutionary response of HIV protease to a drug targeted thereagainst |
EP0862656B1 (en) * | 1995-11-21 | 2001-03-07 | Yale University | Unimolecular segment amplification and detection |
EP0931160B1 (en) * | 1996-09-13 | 2008-12-17 | Alex M. Garvin | Mutation detection using peptide tagged in vitro synthesized proteins |
GB9626074D0 (en) * | 1996-12-16 | 1997-02-05 | Cytocell Ltd | Nucleic acids amplification assay |
-
1998
- 1998-12-08 FR FR9815489A patent/FR2786789B1/en not_active Expired - Fee Related
-
1999
- 1999-12-08 AU AU15679/00A patent/AU772357B2/en not_active Ceased
- 1999-12-08 JP JP2000586946A patent/JP4580104B2/en not_active Expired - Fee Related
- 1999-12-08 AT AT99958276T patent/ATE259884T1/en not_active IP Right Cessation
- 1999-12-08 EP EP99958276A patent/EP1137804B9/en not_active Expired - Lifetime
- 1999-12-08 CA CA002354986A patent/CA2354986A1/en not_active Abandoned
- 1999-12-08 IL IL14363799A patent/IL143637A0/en active IP Right Grant
- 1999-12-08 ES ES99958276T patent/ES2216598T3/en not_active Expired - Lifetime
- 1999-12-08 WO PCT/FR1999/003061 patent/WO2000034513A1/en active IP Right Grant
- 1999-12-08 DE DE69914942T patent/DE69914942T2/en not_active Expired - Lifetime
-
2001
- 2001-06-07 IL IL143637A patent/IL143637A/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
IL143637A (en) | 2006-10-05 |
FR2786789B1 (en) | 2001-12-28 |
ATE259884T1 (en) | 2004-03-15 |
FR2786789A1 (en) | 2000-06-09 |
EP1137804A1 (en) | 2001-10-04 |
JP2002531143A (en) | 2002-09-24 |
ES2216598T3 (en) | 2004-10-16 |
WO2000034513A1 (en) | 2000-06-15 |
EP1137804B9 (en) | 2004-10-06 |
CA2354986A1 (en) | 2000-06-15 |
JP4580104B2 (en) | 2010-11-10 |
AU772357B2 (en) | 2004-04-22 |
EP1137804B1 (en) | 2004-02-18 |
AU1567900A (en) | 2000-06-26 |
DE69914942D1 (en) | 2004-03-25 |
DE69914942T2 (en) | 2004-12-16 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FF | Patent granted | ||
KB | Patent renewed |