IL104157A - Method for obtaining fused cells - Google Patents
Method for obtaining fused cellsInfo
- Publication number
- IL104157A IL104157A IL10415792A IL10415792A IL104157A IL 104157 A IL104157 A IL 104157A IL 10415792 A IL10415792 A IL 10415792A IL 10415792 A IL10415792 A IL 10415792A IL 104157 A IL104157 A IL 104157A
- Authority
- IL
- Israel
- Prior art keywords
- cell
- animal
- medium
- ability
- basal medium
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 34
- 210000004027 cell Anatomy 0.000 claims description 105
- 239000002609 medium Substances 0.000 claims description 37
- 210000004102 animal cell Anatomy 0.000 claims description 32
- 239000007640 basal medium Substances 0.000 claims description 31
- 239000000126 substance Substances 0.000 claims description 23
- 230000002062 proliferating effect Effects 0.000 claims description 20
- 241000282414 Homo sapiens Species 0.000 claims description 16
- 210000004185 liver Anatomy 0.000 claims description 9
- 102000014150 Interferons Human genes 0.000 claims description 7
- 108010050904 Interferons Proteins 0.000 claims description 7
- 210000004408 hybridoma Anatomy 0.000 claims description 7
- 229940079322 interferon Drugs 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 7
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- 206010028980 Neoplasm Diseases 0.000 claims description 3
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- 241000283690 Bos taurus Species 0.000 claims description 2
- 241000282465 Canis Species 0.000 claims description 2
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- 208000031637 Erythroblastic Acute Leukemia Diseases 0.000 claims description 2
- 208000036566 Erythroleukaemia Diseases 0.000 claims description 2
- 241001529936 Murinae Species 0.000 claims description 2
- 208000021841 acute erythroid leukemia Diseases 0.000 claims description 2
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- 238000002474 experimental method Methods 0.000 description 1
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- GVUGOAYIVIDWIO-UFWWTJHBSA-N nepidermin Chemical compound C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H](CS)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CS)NC(=O)[C@H](C)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C(C)C)C(C)C)C1=CC=C(O)C=C1 GVUGOAYIVIDWIO-UFWWTJHBSA-N 0.000 description 1
- 102000013415 peroxidase activity proteins Human genes 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 description 1
- XJMOSONTPMZWPB-UHFFFAOYSA-M propidium iodide Chemical compound [I-].[I-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CCC[N+](C)(CC)CC)=C1C1=CC=CC=C1 XJMOSONTPMZWPB-UHFFFAOYSA-M 0.000 description 1
- XNSAINXGIQZQOO-SRVKXCTJSA-N protirelin Chemical compound NC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)[C@H]1NC(=O)CC1)CC1=CN=CN1 XNSAINXGIQZQOO-SRVKXCTJSA-N 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
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- 150000003839 salts Chemical class 0.000 description 1
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- 230000003248 secreting effect Effects 0.000 description 1
- 230000007651 self-proliferation Effects 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000003270 steroid hormone Substances 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/16—Animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP3356551A JPH0763363B2 (ja) | 1991-12-25 | 1991-12-25 | 融合細胞の取得方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
IL104157A0 IL104157A0 (en) | 1993-05-13 |
IL104157A true IL104157A (en) | 1999-06-20 |
Family
ID=18449596
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IL10415792A IL104157A (en) | 1991-12-25 | 1992-12-18 | Method for obtaining fused cells |
Country Status (9)
Country | Link |
---|---|
US (1) | US5602027A (cs) |
EP (1) | EP0552569B1 (cs) |
JP (1) | JPH0763363B2 (cs) |
KR (1) | KR100280241B1 (cs) |
AU (1) | AU665594B2 (cs) |
DE (1) | DE69230930T2 (cs) |
DK (1) | DK0552569T3 (cs) |
IL (1) | IL104157A (cs) |
TW (1) | TW265364B (cs) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5251936A (en) * | 1991-06-05 | 1993-10-12 | Fitzgibbons Gary W | Overlap check construction or similar business form |
US5643745A (en) * | 1994-02-03 | 1997-07-01 | University Of Hawaii | Heterologous dimeric proteins produced in heterokaryons |
US5683899A (en) | 1994-02-03 | 1997-11-04 | University Of Hawaii | Methods and compositions for combinatorial-based discovery of new multimeric molecules |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4594325A (en) * | 1981-03-26 | 1986-06-10 | The Regents Of The University Of Calif. | High fusion frequency fusible lymphoblastoid cell line |
JPS60141285A (ja) * | 1983-12-29 | 1985-07-26 | Hironori Murakami | ヒトハイブリド−マ作成用親細胞株 |
US4757018A (en) * | 1985-02-11 | 1988-07-12 | Hazleton Biotechnologies, Inc. | Myeloma cell lines and uses thereof |
JP2599123B2 (ja) * | 1985-12-28 | 1997-04-09 | 萩原 義秀 | 新規なヒトbセル・リンパ芽球細胞変異株 |
WO1987005929A1 (en) * | 1986-04-01 | 1987-10-08 | Genelabs Incorporated | Immortalized cells which produce tissue-specific products |
IL87737A (en) * | 1987-09-11 | 1993-08-18 | Genentech Inc | Method for culturing polypeptide factor dependent vertebrate recombinant cells |
-
1991
- 1991-12-25 JP JP3356551A patent/JPH0763363B2/ja not_active Expired - Fee Related
-
1992
- 1992-12-16 AU AU30229/92A patent/AU665594B2/en not_active Ceased
- 1992-12-16 US US07/991,596 patent/US5602027A/en not_active Expired - Fee Related
- 1992-12-18 IL IL10415792A patent/IL104157A/en not_active IP Right Cessation
- 1992-12-24 DE DE69230930T patent/DE69230930T2/de not_active Expired - Fee Related
- 1992-12-24 TW TW081110356A patent/TW265364B/zh active
- 1992-12-24 KR KR1019920025534A patent/KR100280241B1/ko not_active Expired - Fee Related
- 1992-12-24 DK DK92311793T patent/DK0552569T3/da active
- 1992-12-24 EP EP92311793A patent/EP0552569B1/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
EP0552569A1 (en) | 1993-07-28 |
IL104157A0 (en) | 1993-05-13 |
US5602027A (en) | 1997-02-11 |
AU665594B2 (en) | 1996-01-11 |
DK0552569T3 (da) | 2000-07-17 |
JPH0763363B2 (ja) | 1995-07-12 |
TW265364B (cs) | 1995-12-11 |
AU3022992A (en) | 1993-07-01 |
JPH05176763A (ja) | 1993-07-20 |
KR100280241B1 (ko) | 2001-04-02 |
KR930013098A (ko) | 1993-07-21 |
DE69230930D1 (de) | 2000-05-25 |
DE69230930T2 (de) | 2000-08-17 |
EP0552569B1 (en) | 2000-04-19 |
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