IE913070A1 - Novel compounds - Google Patents
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- IE913070A1 IE913070A1 IE307091A IE307091A IE913070A1 IE 913070 A1 IE913070 A1 IE 913070A1 IE 307091 A IE307091 A IE 307091A IE 307091 A IE307091 A IE 307091A IE 913070 A1 IE913070 A1 IE 913070A1
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- C—CHEMISTRY; METALLURGY
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6435—Plasmin (3.4.21.7), i.e. fibrinolysin
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
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- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21007—Plasmin (3.4.21.7), i.e. fibrinolysin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
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Abstract
A hybrid fibrinolytic enzyme, its preparation, pharmaceutical compositions containing it and its use in the treatment of thrombotic disease.
Description
Novel Compounds The present invention relates to a hybrid fibrinolytic enzyme and derivatives thereof, its preparation, pharmaceutical compositions containing it and its use in the treatment of thrombotic disease, in particular acute myocardial infarction.
The sequence of amino acids making up the enzyme tissue-type plasminogen activator (t-PA) and the nucleotide sequence for the cDNA which codes for t-PA are known (see Pennica et al., 1983; Nature, 301, 214). t-PA is known to have fibrinolytic activity.
As used herein, the term tissue-type plasminogen activator (t-PA) denotes a plasminogen activator of the group having the immunological properties defined for t-PA at the XXVIII Meeting of the International Committee on Thrombosis and Haemostasis, Bergamo, Italy, 27 July 1982.
The amino acid sequence of various forms of t-PA are known. The abovementioned Nature 1983 reference discloses the sequence for the L-chain and the mature S-chain forms of t-PA, also discussed by Vehar et al., Biotechnology, 1984, 2, 1051-7 in which the processing of initially formed t-PA by removal of a pro-sequence to give the S-chain form is reported. Pohl et al., FEBS letters, 1984, Vol. 168 No.l, 29-32, refers to the N-terminal multiplicity of t-PA and discloses the U-chain form. The numbering system for the amino acid sequence of t-PA used herein is that described in the Nature 1983 reference for mature (S-chain) t-PA in which the N-terminal serine is numbered 1. By this system, L-chain t-PA has an N-terminal glycine residue at position -3 and U-chain t-PA has an N-terminal valine at position 4.
References to t-PA herein are understood to include all such variant forms.
B3042 -2Native t-PA is composed of a B or light and an A or heavy chain. The B-chain contains the active site of the enzyme. The cleavage site for the conversion of t-PA from the single to the two-chain form is located between residues arg-275 and ile-276. In the two-chain form the chains are held together by a disulphide bridge formed between residues cys-264 in the A-chain and cys-395 in the B-chain.
It has been shown (Ny, T. et al, 1984; Proc. Natl. Acad.
Sci. U.S.A., 81, 5355) that the A chain exhibits a number of structural and functional domains which are homologous to structures found in other plasma proteins: two triple disulphide-bonded structures or kringles, a growth-factor-like domain and a fibronectin-finger-like 15 domain.
The sequence of amino acids making up the enzyme urokinase-type plasminogen activator (u-PA) in its single chain and two chain forms (Verstraete, M. and Collen, D., 1986; Blood, 67, 1529) and the nucleotide sequence for the cDNA which codes for human u-PA (Holmes, W. E. et al, 1985; Bio/technology 3., 923-929) are known. Urokinase-type plasminogen activator is known to have fibrinolytic activity. The two chains of u-PA are termed the A- and B-chain. The B-chain contains the active site of the enzyme. The cleavage site for the conversion of u-PA from the single to the two chain form is located between residues lys-158 and ile-159. In the two chain form the chains are held together by a disulphide bridge formed between residues cys-148 in the A-chain and cys-279 in the B-chain.
As used herein, the term urokinase-type plasminogen activator (u-PA) denotes a plasminogen activator of the group having the immunological properties defined for u-PA at the XXVIII Meeting of the International Committee on Thrombosis and Haemostasis, Bergamo, Italy, 27 July 1982.
B3042 -3The numbering system for the amino acid and nucleotide sequence of u-PA used herein is that described in Holmes, W. E. et al, 1985 (op. cit.) in which the N- terminal serine residue is numbered 1.
In addition to the native forms of t-PA and u-PA described above, various muteins and hybrids are also known, see for example EP-A-0201153, EP-A-0233013, EP-A-0199574, WO 86/01538, EP-A-0227462, EP-A-0253582, WO 86/04351, EP-A-0236040, EP-A-0200451, EP-0225286, DE 3537176, WO 87/04722, WO 90/02798, EP 0299706, WO 89/04368, WO 90/00600, WO 90/02798 and PCT/GB91/00801 (incorporated herein by reference).
References herein to t-PA and u-PA species include both native forms and muteins.
Plasmin is a two-chain serine protease which may be obtained by the cleavage of the single chain precursor, plasminogen, at a specific internal peptide bond. The amino acid sequence of human plasminogen is known (Wiman and Walters (1975) Eur.J. Biochem. 50., 489-494 and 58., 539-547; Wiman (1977) Eur. J. Biochem. .76., 129-137; Sottrup-Jensen et al. (1978) Fibrinolysis and Thrombolysis Vol. 3, 191-209, Raven Press, New York; and Sottrup-Jensen et al. (1978) Atlas of Protein Sequence and Structure Vol. 5, Suppl. 3, p91, National Biomedical Research Foundation, Silver Spring, MD). A partial nucleotide sequence coding for amino acid residues 272-790 of human plasminogen has also been described (Malinowski, D.P. et al., 1984, Biochemistry, 23, 4243-4250). The cleavage site of human plasminogen is located between residues arg-560 and val-561 (according to the sequence numbering of Sottrup-Jensen et al. (1978) Atlas of Protein Sequence (op.cit.)). Two species of plasminogen have been identified ( F.J. Castellino, Chemical Reviews Vol. 81 p431 (1981)): glug which has an N-terminal glutamic acid residue at position 1 and lys77 which has an N-terminal B3042 -4lysine residue at position 77. Glu-plasminogen is also easily converted by limited plasmic digestion to other modified forms with N-terminal valine (val7g) or methionine (metgg) (C. Miyashita, E. Wenzel and M. Heiden, Haemostasis 18, supp.l pp 7-13 (1988)). References to plasminogen herein are understood to include all these species.
A complete nucleotide sequence has also been described (Forsgren, M., et al., 1987, FEBS Letters 213, 254-260).
The nucleotide sequence predicts the existence of an extra, previously unreported, isoleucine residue near the N-terminus of the A-chain. This finding has been independently confirmed (McLean, J.N., et al., 1987, Nature 330, 132-137). Accordingly all sequence numbering (amino acid and nucleotide) below follows Forsgren et al. (1987).
In this numbering sequence the plasminogen cleavage site is located between residues arg-561 and val-562 and the N-terminal modified forms are termed metgg, lys7g and val7g.
Plasminogen has five kringle structures. The region from the first to the last cysteine residue of each kringle structure, residues 84 to 162, 166 to 243, 256 to 333, 358 to 435 and 462 to 541 inclusive will be referred to herein as the ΚχΡ, K2P, K3P, &4P and K5P domains respectively.
According to the present invention there is provided a hybrid plasminogen activator which comprises kringle 5 or kringles 4 and 5 of plasminogen linked to the B-chain of t-PA or u-PA via an amino acid sequence comprising, respectively, the t-PA cleavage site between residues 275 and 276 and the cysteine residue 264 of t-PA or the u-PA cleavage site between residues 158 and 159 and the cysteine residue 148 of u-PA.
B3042 -5It will be understood that by the term 'B-chain' is meant at least that portion of the B-chain containing the functional active centre of t-PA or u-PA, and preferably comprises residues 276-527 or 159-411 respectively.
The linking sequence of amino acids may be introduced synthetically during the preparation of the hybrid plasminogen activator (PA) and/or derived from native sequences .
Native plasminogen includes cysteine residues at positions 548 and 558, C-terminal to plasminogen kringle 5, which participate in the interchain disulphide bonds of the two-chain plasmin form. In the preferred embodiment these residues are not present in the linking sequence.
It will be appreciated that to prevent cleavage of the plasminogen kringle(s) from the t-PA or u-PA B-chain in vivo, the linking sequence should be chosen so as to avoid the presence of a site susceptible to trypsin-like proteolytic cleavage N-terminal to residue cys-264 of t-PA or cys-148 of u-PA, as appropriate.
Where the B-chain of t-PA is employed, the linking sequence of amino acids preferably comprises t-PA residues 264 to 275 inclusive, more preferably residues 262 to 275 inclusive.
Where the B-chain of u-PA is employed, the linking sequence of amino acids preferably comprises u-PA residues 148 to 158 inclusive, more preferably residues 137 to 158 inclusive.
In one preferred aspect, the hybrid PA may be represented symbolically as: B3042 -6mZ4K5Pz5Bt where B1 comprises residues 27 6-527 of t-PA, m is 0 or 1, K4P and KgP represent kringle domains 4 and 5 derived from plasminogen and each of Zg, Z4 and Z$ represents, as appropriate, an optional N-terminal amino acid sequence or a bond or a linking sequence of amino acids which may be introduced synthetically during the preparation of the hybrid PA and/or derived from native plasminogen and/or t-PA sequences, the sequence Zg comprising at least residues cys-264 and arg-275 of t-PA.
In a second preferred aspect, the hybrid PA may be represented symbolically as: (Z3K4p)mz4K5pZ5Bu where Bu comprises residues 159-411 of u-PA and each of Zg, Z4 and Zg represents, as appropriate, an optional N-terminal amino acid sequence or a bond or a linking sequence of amino acids which may be introduced synthetically during the preparation of the hybrid PA and/or derived from native plasminogen and/or u-PA sequences, the sequence Zg comprising at least residues cys-148 and lys-158 of u-PA and m, K4P and KgP are as previously defined.
Where m is 1, the sequence Zg preferably has at its N-terminus the sequence [GARSYQ] or [SYQ] corresponding to the L- and S-chain forms of t-PA, and comprises some or all of the native plasminogen inter-domain sequence between plasminogen kringle domains 3 and 4, preferably plasminogen residues 347-357.
When m is 0, the sequence Z4 preferably has at its N-terminus the sequence [GARSYQ] or [SYQ] corresponding to B3042 -7the L- and S-chain forms of t-PA, and comprises some or all of the native plasminogen inter-domain sequence between plasminogen kringle domains 4 and 5, preferably plasminogen residues 443-461.
Where m is 1, Z^ preferably represents the native plasminogen inter-domain sequence between plasminogen kringle domains 4 and 5. io Suitable sequences (Z$) linking the plasminogen kringle 5 domain to the t-PA B-chain include: 3 [AAPSTCGLRQYSQPQFR] [AAPSTCGLRQYSQPQFQ] [STCGLRQYSQPQFR] (single letter amino acid notation) from which it can be seen that the sequences 1 and 2 consist of residues 542-544 of plasminogen and residues 263 to 275 of t-PA linked by a serine residue. The interposed serine residue can be identified with ser-545 of plasminogen or ser-262 of t-PA. In sequence 2, residue 275 of t-PA has been replaced by glutamine in accordance with EP-A-0233013. The preferred sequence 3 consists of residues 262 to 275 of t-PA.
The preferred sequence (Zg) linking the plasminogen kringle 5 domain to the u-PA B-chain is: [AAPSFPSSPPEELKFQCGQKTLRPRFK] (single letter amino acid notation) from which it can be seen that the sequence consists of residues 542-546 of plasminogen and residues 137 to 158 of u-PA.
B3042 -8The preferred hybrid PA's of the invention have the following structures: 1. [GARSYQ] Pig 347-541[STCGLRQYSQPQFR]Br 5 2. [GARSYQ] Pig 443-541[STCGLRQYSQPQFR]Bfc where Pig x-y represents residues x-y of plasminogen, B^- is as previously defined and the symbols in brackets represent amino acid residues according to the single letter amino acid notation, including one and two chain variants, L- and S-chain variants, and mixtures thereof.
The hybrid PA of the invention may be derivatised to provide pharmaceutically useful conjugates analogous to known PA-containing conjugates, for example: (a) an enzyme-protein conjugate as disclosed in EP-A-0 155 388, in which the catalytic site on the enzyme which is responsible for fibrinolytic activity is blocked by a human protein attached thereto by way of a reversible linking group; (b) an enzyme-protein conjugate as disclosed in EP-A-0152 736, comprising at least one optionally blocked fibrinolytic enzyme linked by way of a site other than the catalytic site responsible for fibrinolytic activity to at least one human protein; (c) a protein-polymer conjugate as disclosed in EP-A-0183503 comprising a pharmaceutically useful protein linked to at least one water soluble polymer by means of a reversible linking group; or (d) an enzyme conjugate as disclosed in EP-A-0184363 comprising a plurality of fibrinolytic enzymes linked B3042 -9together through the active centres thereof by means of a removable blocking group.
The hybrid PA of the invention may take the place of a PA as 5 the enzyme or (human) protein component, as appropriate, of any of the conjugates described above.
The above mentioned derivatives of the hybrid PA may be used in any of the methods and compositions described hereinafter for the hybrid PA itself.
In a further aspect, the invention provides a process for preparing hybrid plasminogen activator according to the invention which process comprises expressing DNA encoding said hybrid plasminogen activator in a recombinant host cell and recovering the hybrid plasminogen activator product.
The DNA polymer comprising a nucleotide sequence that encodes the hybrid PA also forms part of the invention.
The process of the invention may be performed by conventional recombinant techniques such as described in Maniatis et. al., Molecular Cloning - A Laboratory Manual; Cold Spring Harbor, 1982 and DNA Cloning vols I, II and III (D.M. Glover ed., IRL Press Ltd).
In particular, the process may comprise the steps of: i) preparing a replicable expression vector capable, in a host cell, of expressing a DNA polymer comprising a nucleotide sequence that encodes said hybrid plasminogen activator; ii) transforming a host cell with said vector; B3042 -10iii) culturing said transformed host cell under conditions permitting expression of said DNA polymer to produce said hybrid plasminogen activator; and iv) recovering said hybrid plasminogen activator.
The invention also provides a process for preparing the DNA polymer by the condensation of appropriate mono-, di- or oligomeric nucleotide units. io The preparation may be carried out chemically, enzymatically, or by a combination of the two methods,in vitro or in vivo as appropriate. Thus, the DNA polymer may be prepared by the enzymatic ligation of appropriate DNA fragments, by conventional methods such as those described by D. M. Roberts et al in Biochemistry 1985, 24., 5090-5098.
The DNA fragments may be obtained by digestion of DNA containing the required sequences of nucleotides with appropriate restriction enzymes, by chemical synthesis, by enzymatic polymerisation, or by a combination of these methods .
Digestion with restriction enzymes may be performed in an appropriate buffer at a temperature of 20°-70°C, generally in a volume of 50μ1 or less with 0.1-10μg DNA.
Enzymatic polymerisation of DNA may be carried out in vitro using a DNA polymerase such as DNA polymerase I (Klenow fragment) in an appropriate buffer containing the nucleoside triphosphates dATP, dCTP, dGTP and dTTP as required at a temperature of 10°-37°C, generally in a volume of 50μ1 or less.
B3042 -llEnzymatic ligation of DNA fragments may be carried out using a DNA ligase such as T4 DNA ligase in an appropriate buffer at a temperature of 4°C to ambient, generally in a volume of 50μ1 or less.
The chemical synthesis of the DNA polymer or fragments may be carried out by conventional phosphotriester, phosphite or phosphoramidite chemistry, using solid phase techniques such as those described in 'Chemical and Enzymatic Synthesis of Gene Fragments - A Laboratory Manual' (ed. H.G. Gassen and A. Lang), Verlag Chemie, Weinheim (1982),or in other scientific publications, for example M.J. Gait, H.W.D. Matthes, M. Singh, B.S. Sproat, and R.C. Titmas, Nucleic Acids Research, 1982, 10, 6243; B.S. Sproat and W.
Bannwarth, Tetrahedron Letters, 1983, 24, 5771; M.D.
Matteucci and M.H Caruthers, Tetrahedron Letters, 1980, 21, 719; M.D. Matteucci and M.H. Caruthers, Journal of the American Chemical Society, 1981, 103, 3185; S.P. Adams et al., Journal of the American Chemical Society,1983, 105, 661; N.D. Sinha, J. Biernat, J. McMannus, and H. Koester, Nucleic Acids Research, 1984, 12, 4539; and H.W.D. Matthes et al., EMBO Journal, 1984, 3, 801. Preferably an automated DNA synthesizer is employed.
The DNA polymer is preferably prepared by ligating two or more DNA molecules which together comprise a DNA sequence encoding the hybrid PA.
The DNA molecules may be obtained by the digestion with suitable restriction enzymes of vectors carrying the required coding sequences.
The precise structure of the DNA molecules and the way in which they are obtained depends upon the structure of the desired hybrid PA product. The design of a suitable B3042 -12strategy for the construction of the DNA molecule coding for the hybrid PA is a routine matter for the skilled worker in the art.
The expression of the DNA polymer encoding the hybrid PA in a recombinant host cell may be carried out by means of a replicable expression vector capable, in the host cell, of expressing the DNA polymer. The expression vector is novel and also forms part of the invention.
The replicable expression vector may be prepared in accordance with the invention, by cleaving a vector compatible with the host cell to provide a linear DNA segment having an intact replicon, and combining said linear segment with one or more DNA molecules which, together with said linear segment, encode the hybrid PA, under ligating conditions.
The ligation of the linear segment and more than one DNA molecule may be carried out simultaneously or sequentially as desired.
Thus, the DNA polymer may be preformed or formed during the construction of the vector, as desired.
« The choice of vector will be determined in part by the host, which may be a prokaryotic cell, such as E. coli or Streptomyces sp., or a eukaryotic cell, such as a mouse C127, mouse myeloma, human HeLa, Chinese hamster ovary, filamentous or unicellular fungi or insect cell. The host may also be a transgenic animal. Suitable vectors include plasmids, bacteriophages, cosmids and recombinant viruses, derived from, for example, baculoviruses and vaccinia.
The preparation of the replicable expression vector may be carried out conventionally with appropriate enzymes for B3042 -13restriction, polymerisation and ligation of the DNA, by procedures described in, for example, Maniatis et al., cited above. Polymerisation and ligation may be performed as described above for the preparation of the DNA polymer.
Digestion with restriction enzymes may be performed in an appropriate buffer at a temperature of 20°-70°C, generally in a volume of 50μ1 or less with O.l-lC^g DNA.
The recombinant host cell is prepared, in accordance with 10 the invention, by transforming a host cell with a replicable expression vector of the invention under transforming conditions. Suitable transforming conditions are conventional and are described in, for example, Maniatis et al., cited above, or ''DNA Cloning'' Vol. II, D.M. Glover ed., IRL Press Ltd, 1985.
The choice of transforming conditions is determined by the host cell. Thus, a bacterial host such as E. coli may be treated with a solution of CaCl2 (Cohen et al, Proc. Nat.
Acad. Sci., 1973, 69., 2110) or with a solution comprising a mixture of RbCl, MnCl2, potassium acetate and glycerol, and then with 3-[N-morpholino]-propane-sulphonic acid, RbCl and glycerol. Mammalian cells in culture may be transformed by calcium co-precipitation of the vector DNA onto the cells.
The invention also extends to a host cell transformed with a replicable expression vector of the invention.
Culturing the transformed host cell under conditions permitting expression of the DNA polymer is carried out conventionally, as described in, for example, Maniatis et al and ''DNA Cloning'' cited above. Thus, preferably the cell is supplied with nutrient and cultured at a temperature below 45°C.
B3042 -14The hybrid PA expression product is recovered by conventional methods according to the host cell. Thus, where the host cell is bacterial, such as E. coli it may be lysed physically, chemically or enzymatically and the protein product isolated from the resulting lysate. Where the host cell is mammalian, the product may generally be isolated from the nutrient medium.
The DNA polymer may be assembled into vectors designed for 10 isolation of stable transformed mammalian cell lines expressing the hybrid PA; e.g. bovine papillomavirus vectors or amplified vectors in Chinese hamster ovary cells (DNA cloning Vol.II D.M. Glover ed. IRL Press 1985; Kaufman, R.J. et al., Molecular and Cellular Biology 5, 1750-1759, 1985; Pavlakis G.N. and Hamer, D.H., Proceedings of the National Academy of Sciences (USA) 80, 397-401, 1983; Goeddel, D.V. et al., European Patent Application No. 0093619, 1983).
It will be appreciated that, depending upon the host cell, the hybrid PA prepared in accordance with the invention may be glycosylated to varying degrees. Furthermore, as observed by Pohl et.al., Biochemistry, 1984, 23, 3701-3707, varying degress of glycosylation may also be found in unmodified, naturally occurring t-PA. Plasminogen also exhibits varying degrees of glycosylation (Hayes M.L, J.
Biol. Chem. 254: 8768, 1979). Mutant forms of the hybrid PA are also contemplated in which glycosylation sites are removed by genetic engineering techniques, for example as taught in EP-A-0225286, DE-3537176, WO 87/04722, EP-0299706 or WO 89/04368. The hybrid PA of the invention is understood to include such glycosylated variations.
It will also be appreciated that, depending upon the expression conditions, the hybrid PA prepared in accordance with the invention may exist in the single or two chain forms or mixtures thereof. The invention extends to all B3042 -15such forms.
The hybrid PA of the invention comprises the B-chain of native t-PA or u-PA linked to an A-chain comprising kringle or kringles 4 and 5 derived from plasminogen via a linking sequence of amino acids comprising residues 264 and 275 of t-PA or residues 158 and 148 of u-PA.
This hybrid PA A-chain may be employed as one chain of a 10 fibrinolytically active hybrid protein such as disclosed in EP-0 155 387. The hybrid A-chain, DNA encoding the hybrid A-chain and a hybrid protein comprising the hybrid A-chain linked to the B-chain of a fibrinolytically active protease, the catalytic site of which is optionally blocked by a removable blocking group, all form part of the invention.
The hybrid A-chain may be prepared by separation from the B-chain thereof by mild reduction. Alternatively the hybrid A-chain may be prepared by expressing DNA coding therefor in a recombinant host cell and recovering the hybrid A-chain product. The hybrid protein comprising the hybrid A-chain linked to the B-chain of a fibrinolytically active protease may be prepared by (a) mixing said A- and B-chains under oxidative conditions; or (b) ligating DNA encoding said A-chain to DNA encoding said B-chain and expressing the ligated DNA in a prokaryote or eukaryote host; and thereafter optionally blocking the catalytic site of the hybrid protein with a removable blocking group. The oxidation and reduction conditions are as generally described in EP-A-0 155 387.
The resulting hybrid protein may be used in any of the methods and compositions described hereinafter for the hybrid PA itself.
B3042 -16The hybrid PA of the invention or conjugate thereof can be further derivatised such that any catalytic site essential for fibrinolytic activity is optionally blocked by a removable blocking group.
As used herein the expression 'removable blocking group' includes groups which are removable by hydrolysis at a rate such that the pseudo-first order rate constant for hydrolysis is in the range of 10 ° sec to 10 sec , more preferably 10 ° sec 1 to 10 sec x, in isotonic aqueous media at pH 7.4 at 37°C.
Such blocking groups and blocking reactions are described in European Patent No.0009879 and EP 0297882 and include acyl groups such as optionally substituted benzoyl or optionally substituted acryloyl.
Suitable optional substituents for benzoyl blocking groups include halogen, C^_g alkyl, Cj_g alkoxy, C-^_g alkanoyloxy, Cg_g alkanoylamino, amino or p-guanidino.
Suitable optional substituents for acryloyl blocking groups include Cg_g alkyl, furyl, phenyl or Cg_g alkylphenyl.
In one aspect, the removable blocking group is a 2-aminobenzoyl group substituted in the 3- or 4-position with a halogen atom and optionally further substituted with one or more weakly electron-withdrawing or electon-donating groups, wherein the pseudo first order rate constant for hydrolysis of the derivative is in the range 6.0 χ 10-5 to 4.0 χ 10-4 sec-·'· when measured in a buffer system consisting of 0.05M sodium phosphate, 0.IM sodium chloride, 0.01% v/v detergent comprising polyoxyethylenesorbitan monoleate having a molecular weight of approximately 1300, at pH 7.4 at 37°C.
B3042 -17Preferably the pseudo first order rate constant for hydrolysis of the derivative is in the range 6.0 x 10-5 to 2.75 x 104 s_1, preferably 6.0 χ 10-5 to 2.5 χ 10-4 s-1, more preferably 6.0 χ 10-3 to 2.0 x 10-4 s~l, still more preferably 6.0 χ 10-3 to 1.5 χ 10-4 s"l and most preferably 7.0 x IO'5 to 1.5 x 10-4 s-1.
Preferably, the 2-aminobenzoyl group is substituted with a halogen atom in the 4-position.
Preferably, the halogen atom is fluorine, chlorine or bromine.
When the group is further substituted, preferred groups include C3_g alkyl, C3_g alkoxy and C]__g alkenyl substituents in the 3- or 5-positions of the ring.
Examples of the blocking group include 4-fluoro-2aminobenzoyl, 4-chloro-2-aminobenzoyl, 4-bromo-2-aminobenzoyl and p-methoxybenzoyl.
The hybrid PA and derivatives of the invention are suitably administered in the form of a pharmaceutical composition.
Accordingly the present invention also provides a pharmaceutical composition comprising a hybrid PA or derivative of the invention in combination with a pharmaceutically acceptable carrier.
The compositions according to the invention may be formulated in accordance with routine procedures as pharmaceutical compositions adapted for intravenous administration to human beings.
Typically compositions for intravenous administration are solutions of the sterile enzyme in sterile isotonic aqueous B3042 -18buffer. Where necessary the composition may also include a solubilising agent to keep the hybrid PA or derivative in solution and a local anaesthetic such as iignocaine to ease pain at the site of injection. Generally, the hybrid PA or derivative will be supplied in unit dosage form for example as a dry powder or water free concentrate in a hermetically sealed container such as an ampoule or sachette indicating the quantity of protein in activity units. Where composition comprises a derivative of the invention or where the hybrid PA includes a removable blocking group, an indication of the time within which the free protein will be liberated may be given. Where the protein is to be administered by infusion, it will be dispensed with an infusion bottle containing sterile pharmaceutical grade 'Water for Injection' or saline. Where the protein is to be administered by injection, it is dispensed with an ampoule of sterile water for injection or saline. The injectable or infusable composition will be made up by mixing the ingredients prior to administration.
The quantity of material administered will depend upon the amount of fibrinolysis required and the speed with which it is required, the seriousness of the thromboembolic condition and position and size of the clot. The precise dose to be employed and mode of administration must per force in view of the nature of the complaint be decided according to the circumstances by the physician supervising treatment. However, in general, a patient being treated for a thrombus will generally receive a daily dose of from 0.01 to 10 mg/kg of body weight, such as 0.10 to 2.0mg/kg, either by injection in for example up to five doses or by infusion.
Within the above indicated dosage range, no adverse toxicological effects are indicated with the compounds of the invention.
B3042 -19Accordingly, in a further aspect of the invention there is provided a method of treating thrombotic diseases, which comprises administering to the sufferer an effective non-toxic amount of hybrid PA or derivative of the invention.
In another aspect the invention provides the use of a hybrid PA or derivative of the invention for the manufacture of a medicament for the treatment of thrombotic diseases.
The invention also provides a hybrid PA or derivative of the invention for use as an active therapeutic substance and in particular for use in the treatment of thrombotic diseases.
The following Methods and Examples illustrate the invention.
B3042 -20I. General Methods used in Examples (i) DNA cleavage In general the cleavage of about lgg of plasmid DNA or DNA fragments was effected using about 5 units of a restriction enzyme (or enzymes) in about 20μ1 of an appropriate buffer solution. (ii) Ligation of DNA fragments Ligation reactions were carried out as described in Maniatis et al, (Molecular Cloning - A Laboratory Manual, Cold Spring Harbor Laboratory, 1982). (iii) Transformation of plasmid DNA into E.coli HB101 cells used competent HB101 supplied by Gibco BRL (Paisley, Scotland), according to the manufacturers instructions. (iv) Plasmid preparation Preparation of plasmid DNA was carried out as described in Maniatis et al, (Molecular Cloning - A Laboratory Manual, Cold Spring Harbor Laboratory, (1982)). (v) Isolation of DNA fragments from low-melting-point (LMP) agarose gels DNA fragments were isolated from LMP agarose gels as described by Maniatis et al, (Molecular Cloning-A Laboratory Manual, Cold Spring Harbor Laboratory, 1982). Alternatively the excised gel band was purified using GENECLEANtm, B3042 -21(Stratech Scientific, London) used according to the manufacturers instructions. (vi) Oligonucleotides 5 Oligonucleotides were made on Applied Biosystems 381A DNA Synthesizer according to the manufacturers instructions.
When used in plasmid construction the oligonucleotides were annealed by heating together at 95°C for 5 minutes and cooling slowly to room temperature. The annealed oligonucleotides were then ready for ligation. (vii) DNA sequencing by double-strand method . . . TM Sequencing was carried out using 'Sequenase (United States Biochemical Corporation) essentially according to the manufacturers instructions. (viii) Transient expression of plasminogen activators from HeLa cells (a) Small-scale Cell preparation: cells were trypsinised and plated out at c approx. 2.4 x 10 cells per 35mm dish and incubated in 1.5ml growth medium (this is Hepes buffered RPMI 1640 medium (041-02400) containing 10% Serum (021-06010), 2% sodium bicarbonate solution (043-05080),; Gibco, Paisley, Scotland) at 37°C in a humidified incubator in an atmosphere of 5% CO2/95% air. After 72h the cells were refed, and used for transfection 24h later.
Transfection procedure: Cultures were changed to Eagles MEM (041-01095), 10% serum (021-06010), and 1% non-essential amino acids (043-01140) 3h before transfection. The transfections used calcium coprecipitation as described in B3042 -22'DNA Cloning' Ed. D.M. Glover (Chap. 15, C. Gorman). Glycerol shock and 5mM butyrate treatments were used. Plasminogen activator(s) secreted by transfected cells was harvested in 1.0ml RPMI 1640 medium (as above, but lacking serum) + 4% Soybean Peptone. (b) Large-scale Cell preparation: cells were trypsinised and plated out at a density of approx. 2.5 χ 10θ cells per 175cm2 flask in 30ml growth medium (above). After 72h an extra 25ml of growth medium was added and the cells were used for DNA transfection 24h later (as above). 25ml of harvest medium were used per flask.
Alternatively the cells were plated at a density of approximately 2.0 x 10^ cells per flask and 25ml of growth medium was added after 96h incubation and the cells used as above.
The two seeding rates and feed times used in the small and large-scale protocols were designed to allow convenient timing of experiments. Both sets of protocols allow efficient expression of activator(s). (ix) Chromogenic substrate assays Hybrid was assayed against the chromogenic substrate S-2288 (KabiVitrum, Sweden) at a substrate concentration of ImM in 0.1 M triethanolamine.HCl pH 8.0 at 25°C. An SU is defined as the amount of activity that gives an O.D. increase at 405nm of 0.001/min in 0.5 ml substrate in a 1 cm pathlength cell.
In another form of the assay, specifically designed for the semi-quantitative assay of chromatography column fractions, 10μ1 of each fraction was mixed with ΙΟΟμΙ ImM S-2288 (as B3042 -23above) in wells of a microtitre plate and the plate incubated at 37°C until such time as yellow colour was visible. The solutions were read at 410 nm using a Dynatech MR700 Microplate reader. (x) Fibrinolytic activity assay The fibrinolytic activity of hybrid plasminogen activator solutions was measured on human plasminogen-containing fibrin plates as described (Dodd, I., and Carr, K., Thrombosis Res. 1989 55 79-85). Dose-responses of hybrid plasminogen activators had slightly different slopes to those of the tissue-type plasminogen activator standard so all activities are approximate. Activities are expressed in IU with reference to the 2nd International standard for t-PA, Lot 86/670, unless otherwise stated. (xi) Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE) SDS PAGE was carried out to determine the apparent molecular weight (s) of the hybrid plasminogen activators using essentially the method of Laemmli (Nature 1970 227 680-685). The activators were identified either by staining for protein or by a fibrin zymography technique (Dodd, I. et al Thromb. Haemostasis 1986, 55 94-97). Using these methods it was generally possible to determine chain nature (sc v tc). (xii) Rate constant determinations Acyl-enzyme (ca. 20 pmol, 10μ1) was added to a solution of S-2288 (0.5ml of 1.OmM in 0.05M sodium phosphate, 0.1M NaCl, 0.01% w/v Tween 80 pH (37°C) 7.4) in a spectrophotometer cuvette thermostatted at 37°C. Absorbance readings at B3042 -24405nm were recorded at 1.0 min intervals for 30 min and on-board software (Beckman Inc.) used to calculate the rate of change of absorbance over each successive 1 min interval. As deacylation proceeded in the cuvette, the rate of change of A405nm increased with time. Rate data obtained when the absorbance exceeded a value of 0.8 were not used because of the effect of substrate depletion. The set of rate determinations were fitted to the following monoexponential function: f(t) = Ao + (Smax - Ao) x (l-e'kt) where AQ is the initial activity of the acyl-enzyme and Amax is the maximum activity possible after deacylation and was determined by deacylation of an aliquot of acyl-enzyme in 0.1M Tris. HCl, 20% w/v glycerol, 0.14M NaCl, 0.01% w/v Tween 80 pH 7.4 at 37°C for lh followed by amidolytic assay with S-2288 under the above conditions (i.e. phosphate buffer pH 7.4, 37°C). A and K, the first order deacylation rate constant, were treated as unknowns in the fitting process and were derived by non-linear regression analysis on a VAX 11/750 computer.
II. Identification of nucleotides, amino acid residues, N-termini, protein domains and chain nature in the examples (i) Sequences All t-PA numbering as in Pennica et al (1983) op. cit.; plasminogen amino acid numbering based on Sottrup-Jensen et al (1978) Atlas of Protein Sequence and Structure Vol. 5, Suppl. 3, p91, National Biomedical Research Foundation, Silver Spring, MD., but updated to include the extra amino acid residue identified by Forsgren, M. et al (1987) FEBS B3042 -25Letters, 213, 254-260. Plasminogen nucleotide sequences as in Forsgren et al. (op.cit.). (ii) Protein Domains 5 The protein domains described in the examples have been abbreviated for convenience and are defined as follows :1. B1 t-PA amino acid residues 276 to 527 inclusive. 2. K^P = plasminogen amino acid residues 358 to 435 inclusive.
K^P = plasminogen amino acid residues 462 to 541 inclusive . (iii) Chain nature sc, indicates that the protein is in single chain form. tc, indicates that the protein is in two chain form. (iv) Vectors pTRE12 - (EP-0201153)-basic expression vector 25 pTRE15 - (EP-0201153)-encodes wild-type t-PA pUC8 - a commercially available vector (Gibco-BRL) containing a multiple cloning site and a gene that confers ampicillin resistance.
B3042 -26Example 1 Construction of pDH55 encoding SYQ/Plasminogen 347-541/t-PA 262-527 (H55) The construction of pDH55 was carried out as a two step process. This plasmid.comprises a cDNA encoding the t-PA signal sequence (-35 to -1) linked to the above hybrid plasminogen activator. The restriction sites used below were located as follows :Styl : plasminogen nucleotide 1210 Sstl : t-PA nucleotide 1417 BamHI : located in SV40 polyA/t intron fragment of pTRE15 Bglll : t-PA nucleotide 187 a) Construction of the plasmid pDH55i (a holding vector containing the sequence encoding SYQ/Plasminogen 347-541/t-PA 262-410) Two fragments were prepared by restriction digestion and agarose gel electrophoresis. These fragments were as follows .
Fragment 1: was the large fragment from an Sstl/Bglll digest of a pUC8 derivative containing a modified multiple cloning site.
Structure of the modified cloning site:IE 913070 B3042 -27Hindlll Sstl ' AGCTTGGGCGCCTTCATTTCCCTCCTCTCCTCCAGAAGAGCTCAAATTT 3' ACCCGCGGAAGTAAAGGGAGGAGAGGAGGTCTTCTCGAGTTTAAA Bglll Hindlll cagtgtggccagatcta x GTCACACCGGTCTAGATTCGA Fragment 2: was an approximately lkb Styl/Sstl fragment from 10 pTRH37 (as described in EP-A 0297 882) encoding most of K4P, KgP and part of Β**.
These two fragments were ligated together with an oligonucleotide linker (Linker 3) to form plasmid pDH55i. Linker 3 (designed to encode the tripeptide SYQ and amino-acid residues 347-359 of plasminogen) was formed by annealing two oligonucleotides (A) and (B) of sequence:5' GATCTTACCAAGCACCACCTGAGCTAACCCCTGTGGTCCAGGACTGCTAC (A) ' CATGGTAGCAGTCCTGGACCACAGGGGTTAGCTCAGGTGGTGCTTGGTAA (B) The DNA was transformed into E.coli HB101 cells. A plasmid 25 (pDH55i) was isolated which has the structure shown in Fig.1. b) Construction of the plasmid pDH55 Three fragments were prepared by restriction digestion and agarose gel electrophoresis. These fragments were as follows : Fragment 4: was an approximately lkb Bglll/Sstl fragment from pDH55i encoding the first three amino acids (SYQ) of t-PA, residues 347-357 of plasminogen, K4pKgP and part of B3042 -28Bfc .
Fragment 5: was an approximately 1.6kb Sstl/BamHl fragment from pTRH37 encoding the C-terminal part of B^· and vector sequences.
Fragment 6: was a BamHl/Bglll fragment derived from pTRE15 encoding vector sequences and the t-PA signal sequence.
These three fragments were ligated together and transformed into E.coli HB101 cells.
A plasmid was isolated which has the structure shown in Fig.2. The plasmid, when introduced into HeLa cells, directed the expression of a novel plasminogen activator.
Example 2 Purification and Characterisation of H55 Protein Conditioned medium from twenty 175cm HeLa cultures transfected with the plasmid pDH55 was centrifuged at approximately 9000g for 30 min. The supernatant (480ml) was buffer-exchanged into PBS'A' (Dulbecco)/0.01% Tween 80 pH 7.4 using a column (i.d., 90mm,-h,226 mm) of Sephadex G25 and a 710ml fraction eluting immediately after the void volume of the column was obtained. The 710 ml fraction was then purified in a similar way to that described for t-PA (Dodd, I. et al FEBS Lett., (1986) 209 13-17). The zinc chelate and lysine Sepharose Fast Flow columns had volumes of 90ml and 10ml respectively. Protein H55 was dissociated from the lysine Sepharose column using a 0.5M arginine-containing buffer; peak H55 - containing fractions were identified by a microtitre plate S2288 assay and were pooled and were ultrafiltered using a membrane with a nominal molecular weight cut off of 10,000 (YM10, Amicon) to a final volume of B3042 -292.2ml. This retentate was regarded as the H55 product.
Fibrin plate assay showed that the original, conditioned harvest medium contained approximately 17000 IU and that the product contained approximately 22000 IU. This difference is believed to be within the natural error in the assay.
Analysis of the product by SDS PAGE followed by protein staining showed a major band at approx apparent Mr 60,000 (non-reduced) or two major bands at Mr 36,000 and 31,000 (reduced). The Mr 36,000 band is known to be the t-PA B-chain moiety; the 31,000 band is presumed to be the K4P+K5P moiety. These results suggest the majority of the material is in the two-chain form.
Analysis of the retentate by SDS PAGE followed by fibrin zymography indicated a major (approximately 95%) fibrinolytic species at apparent Mr 60,000; so the fibrinolytic activity and the major stainable band co-migrate on SDS PAGE.
Example 3 Synthesis of N,N-dimethyl-4-aminobenzoyl two chain H55 (DAB-H55) 1.0ml of the ultrafiltered retentate described in Example 2 (containing 9900 IU;10,000 SU; nominal 0.8nmoles); was mixed with 0.48ml 0.02M Tris/0.2M NaCl/0.2M arginine/0.01% Tween 80 pH 7.0 (Buffer A) and 7.5μ1 20mM 4amidinophenyl-N,N/ dimethyl^-Qaminobenzoate. HCI (dissolved in DMSO). The mixture was incubated for lh at 25°C and the S2288 activity of the solution measured; only 2% of the input activity remained. The mixture was buffer-exchanged into Buffer A using a prepacked column (PD10) of Sephadex G25. The final product had a volume of 2.5ml. Fibrin plate assay showed B3042 -30the solution contained 8000 IU; the S2288 activity was 450 SU. These figures indicate that the activity is being regenerated during the longer incubation on the fibrin plates i.e., that deacylation is occurring.
Example 4 Construction of pDH56 encoding SYQ/Plasminogen 443-541/t-PA 262-527 (H56) The construction of pDH56 was carried out as a two step process. This plasmid comprises a cDNA encoding the t-PA signal sequence (-35 to -1) linked to the above hybrid plasminogen activator. The restriction sites used during construction were as follows:HinFl: plasminogen nucleotide 1498 AlwNl: t-PA nucleotide 1130 Sstl : t-PA nucleotide 1417 BamHI: located in SV40 polyA/t intron fragment of pTRE15 Bglll: t-PA nucleotide 187 a) Construction of the plasmid pDH56i (a holding vector 25 containing the seguence encoding SYQ/Plasminogen 443-541/t-PA 262-410) Three fragments were prepared by restriction digestion and agarose gel electrophoresis. These fragments were as follows :Fragment 1: was from an Sstl/Bglll digest of a pUC8 derivative containing a modified linker region, (as described in Example 1).
B3042 -31Fragment 7: was an approximately 411bp HinFl/AlwNl fragment from pTRH37 (as described in EP-A-0297 882) encoding part of the K4P-K5P bridge, the whole of K^P and part of B^ .
Fragment 8: was an approximately 292bp AlwNl/Sstl fragment from pTRH37 (as described in EP-A-0297 882) encoding part of Be .
These three fragments were ligated together with an 10 oligonucleotide linker (Linker 9) to form plasmid pDH56i.
Linker 9 (designed to encode the tripeptide SYQ and amino-acid residues 443-455 of plasminogen) was formed by annealing two oligonucleotides C and D of sequence :15 5' (C) GATCTTACCAAGTAGCACCTCCGCCTGTTGTCCTGCTTCCAGATGTAGAG 3' ' (D) AGTCTCTACATCTGGAAGCAGGACAACAGGCGGAGGTGCTACTTGGTAA 3' The DNA was transformed into E.coli HB101 cells. A plasmid (pDH56i) was isolated which has the structure shown in Fig.3. b) Construction of the plasmid pDH56 Three fragments were prepared by restriction digestion and agarose gel electrophoresis. These fragments were as follows :Fragment 10: was an approximately 750bp Bglll/Sstl fragment from pDH56i encoding the first three amino acids of t-PA (SYQ), residues 443 to 461 of plasminogen, K^P and part of B1.
Fragment 5: was an approximately 1.6kb Sstl/BamHl fragment from pTRH37 encoding the C-terminal part of Bfc and vector B3042 -32sequences. (As described in Example 1) Fragment 6: was a BamHI/BglII fragment derived from pTRE15 encoding vector sequences and the t-PA signal sequence. (As described in Example 1) These three fragments were ligated together and transformed into E.coli HB101 cells.
A plasmid was isolated (pDH56) which has the structure shown in Fig.4. The plasmid, when introduced into HeLa cells, directed the expression of a novel plasminogen activator.
Example 5 Purification and characterisation of H56 protein Two purifications were carried out, each resulting in a batch of H56 that was characterised extensively. Both purifications were experimental in that it was not clear which affinity columns were best employed; this led to H56 protein not adsorbing to columns and thus rather complicated purification schemes (not helped by affinity matrices performing poorly). For these reasons, the following descriptions indicate the generality of the purifications factors considered unimportant for the actual purification of H56 are not detailed (e.g. a batch of lysine Sepharose used on one occasion did not adsorb the H56 protein; the reason is not known). (a) Approximately 500ml conditioned medium from HeLa cultures transfected with the plasmid pDH56 was centrifuged at approximately 9000g for 30 min. The supernatant was buffer-exchanged into PBS 'A' (Dulbecco)/0.01% Tween 80 pH B3042 -337.4 (PBS/TW) using a column of Sephadex G25 (Vt 1500ml) and the 780 ml sample eluting immediately after the void volume of the column was retained.
The 780ml sample was chromatographed on zinc chelate Sepharose (see Example 2) and aminohexyl Sepharose 4B (AH Sepharose; Sigma chem.Co.). The latter chromatography was carried out as follows.
The column (i.d., 15 mm; h, 45 mm; Vt, 8.0 ml) was equilibrated with PBS/TW. The imidazole-eluted fraction from the zinc chelate column was applied and was washed through with PBS/TW. H56 protein was desorbed using 0.02M Tris/0.5M NaCl/0.5M L-arginine/0.01% Tween 80 pH 7.0. All parts of the chromatography were at 4°C at approximately 100 cm h-1. Active fractions (containing H56) were identified using S2288 and then concentrated by stirred-cell ultrafiltration (YM10, Amicon Ltd). The ultrafiltered retentate was regarded as the product.
The product showed a dose-response relationship on human fibrin plates slightly different to that of t-PA and exhibited a single major band of fibrinolytic activity at apparent Mr 40,000 on SDS PAGE followed by fibrin zymography. This band had the same Mr as a doublet, possibly triplet, of polypeptides detected after probing Western blots of H56 with a monoclonal directed at the B-chain of t-PA (ESP2, BioScot, U.K.) or an anti-t-PA B chain Ig G preparation (Dodd, I. et al, Thrombos.
Haemostos., 1986 55 94). (b) Approximately 500ml conditioned media was prepared and chromatographed on Sephadex G25 and zinc chelate Sepharose essentially as described in (a). Some of the H56 was partially purified on AH-Sepharose and p-aminobenzamidine Sepharose CL4B (pABA Sepharose; Pierce Chem. Co.) resulting in three active fractions. These were pooled, ultra B3042 -34filtered (YM10) and the retentate clarified by centrifugation (10,OOOg/lOmin). The supernatant was buffer-exchanged into PBS/TW using Sephadex G25 (PD10) and then purified on freshly autoclaved pABA Sepharose (Vt 11ml) using the same protocol as for AH Sepharose described in (a). Material that was eluted from the pABA Sepharose column by the 0.5M arginine buffer was concentrated (stirred-cell ultrafiltration; YM10) and buffer-exchanged into 0.05M sodium phosphate/0.IM sodium chloride/lOmg ml-1 Γη3ηηϊίο1/50μΜ E-amino caproic acid/0.01% Tween 80 pH7.4 (Sephadex G25, PD10). The buffer-exchanged material was regarded as the product.
The dose-response of the product on fibrin plates was approximately parallel to that of t-PA; the product contained approximately 3000 IU/ml. SDS PAGE/fibrin zymography and Western blotting studies revealed similar pictures to those obtained for product (a). SDS PAGE (non-reduced) followed by silver staining also showed a major band in the approximate Mr 40,000 region.
Example 6 Synthesis of N,N-dimethyl-4-aminobenzoyl two-chain H56 (DAB H56) 2.1ml H56 (1200 IU/ml) from Example 5 (a) was mixed at 25°C with 6μ1 4'-amidinophenyl-N,N-dimethyl-4-aminobenzoate.HCI (AP-DAB; 2mM in DMSO) and incubated at 25°C. Additional aliquots of 20mM AP-DAB were added at 30 min (2μ1) and 60 min (3μ1). At 90 min 6 per cent of the original amidolytic activity remained. The mixture was buffer-exchanged into 0.02M Tris/0.2M NaCl/0.2M L-arginine/0.01% Tween 80 pH 7.0 (2.5ml) and aliquoted and stored at -40°C.
’In-cuvette' deacylation (see General Methods, (xii)) revealed that 90 per cent of the product was in the B3042 -35acylated (DAB) form. Under the conditions of the deacylation the material had a deacylation half-life of 105 min.
Example 7 Expression of H55 protein in dhfr~Chinese hamster ovary (CHO) cells The strategy used for expression of H55 in CHO DXB11 cells, using an amplifiable dhfr vector, has been described previously in EPA 0297 882.
The cDNA encoding H55 was recovered from pDH55 as a 3.2kb BamHI/MluI fragment. This fragment was subcloned into pTRHll (EPA 0297 882) replacing the original 4.lkb Mlul/BamHI fragment (which encoded protein H204). The new plasmid was called pDH17. In pDH17, the hybrid and dhfr transcription cassettes are opposed i.e. converge at their 3' ends.
A second plasmid, pDH16, was also prepared. In this plasmid the whole Xhol fragment carrying the H55 transcription cassette (including RSVLTR and SV40 elements : depicted in EPA 0297 882, pTRH71) is reversed with respect to that in pDH17. The transcription cassettes for dhfr and H55 are therefore transcribed in tandem.
Cell preparation CHO cells were trypsinised and plated out at 6 χ 103 per 90 mm dish and left in growth medium [Hams F12 nutrient media (041-1765) with 1% stock penicillin/streptomycin (043-5070) and 10% foetal calf serum (013-6290); Gibco, Paisley, Scotland] at 37°C in a humidified incubator, in an atmosphere of 5% CO2/95% air. After 18 hrs the growth medium was replaced with transfection medium [Eagles MEM B3042 -36(041-1095) with 1% non-essential amino acids (043-1140), 1% stock penicillin/streptomycin (043-5070), and 10% newborn calf serum (021-6010); Gibco, Paisley, Scotland], After a further 2 hrs the cells were used for DNA transfection.
Transfection Procedure The transfection procedure, carried out in transfection medium, used calcium coprecipitation and glycerol shock as described in DNA Cloning Volume II (Ed. D.M. Glover; chapter 6, C. Gorman). Following transfection the cells were maintained in growth medium for 48 hrs under growth conditions (as above), prior to the selection procedure.
Selection Forty eight hours post-transfection the cells were medium changed into selective medium [aMEM (041-2561) with 2% stock glutamine (043-5030), 1% stock penicillin/streptomycin (043-5070) and 10% dialysed foetal calf serum (063-6300); Gibco, Paisley, Scotland]. The cells were maintained in selective medium for 8-10 days until colonies of dhfr+ cells appeared.
Isolated colonies were grown to confluency in 25 cm flasks and harvested in serum-free medium for 24 hours. Fibrinolytically active protein was detected by fibrin plate assay.
Example 8 Synthesis of 4-anisoyl-t-PA 1-3/plasminogen 443-541/t-PA 262-527 Purified H56 protein from Example 5 (nominal 0.34 nmoles) in 0.05M NaH2PO4.2H20/0.1M NaCl/0.01% Tween 80/10 mgml-1 B3042 -37mannitol/5C^M E-ACA pH 7.4 (0.84ml) was treated with 4-amidinophenyl-4-anisate. HCl (6.8 nmoles; 6.8μ1) at 25°C. After 90 min the amidolytic activity of the preparation had decreased to 5% of the original activity.
The material was buffer-exchanged using Sephadex G25 (PD10) into 0.02M Tris/0.2M NaCl/0.2M L-Arginine/0.01% Tween 80 pH 7.0 and stored at -40°C.
Example 9 Synthesis of 4-anisoyl-t-PA 1-3/plasminogen 347-541/t-PA 262-527 Purified H55 protein from Example 2 (nominal 0.16 nmoles) in 0.02M Tris/0.5M NaCl/0.5M L-Arginine/0.01% Tween 80 pH 7.4 (0.3ml) was treated with 4-amidinophenyl-4-anisate. HCl (3.2 nmoles; 3.2μ1) at 25°C. After 90 min the amidolytic activity of the preparation had decreased to <2% of the original activity.
The material was buffer-exchanged into 0.02M Tris/0.2M NaCl/0.2M L-Arginine/0.01% Tween 80 pH 7.0 using Sephadex G25 (PD10) and stored at -40°C.
B3042 Example. L.Q Expression of H56 using a baculovirus expression system The elements used in expressing H56 are: pAcCL29 Vector: this is based on pAcYMl (an expression vector in which a unique Bam Hl cloning site has been positioned so as to maximise expression using the polyhedrin promoter: Matsura, Y, Possee R.D. Overton, H.A. and Bishop D.H.L [1987] J.Gen.Virol 68. 1233-1250). pAcCL29 (Livingstone, C. and Jones I (1989) NAR 17. 2366) was derived from pAcYMl as follows; an approximately 5Kb EcoRI-XhoI fragment coding for all the signals necessary for efficient expression and recombination were removed from pAcYMl, blunt ended and ligated into in-filled EcoRl Hind III sites in pUC118 (Vieira. J. and Messing J, [1987] Meth. Enzy. 153 3-11).
Wild type virus is: Autographa californlca. nuclear polyhedrosis virus (AcNPV).
Cell line .: IPLB Sf21 derived from Spodoptera frugiperda. 25 (Vaughan, J.L., Goodwin, R.H., Thompkins, G.J. and McCawley, P. 1977: In Vitro 13, 213-217) Methad An approx. 2.5 kb Hind III - Bam HI (Hind III located at 5' end of untranslated region. Bam HI located 3' to coding region) fragment from pDH56 (Example 4) was cloned into the Bam HI site of the baculovirus transfer vector pAcCL29 using the following linker: ' 3’ GATCCGATATCA 3' GCTATAGTTCGA 5' to give pDB769.
B3042 - 39 This new recombinant baculovirus was used to infect Sf 21 cells and express the H56 gene as a fibrinolytically active product; standard methods were used (Summers, M.D. and Smith, G.E., 1987: A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures, Texas Agricultural Experiment Station Bulletin No. 1555, and modifications described in Page, M.J. and Murphy, V.F., 1988: in J.M. Walker (ed) Methods in Molecular Biology, Vol. 5).
Example 11 Expression of H55 in E.coli 15 (a) : Construction of the E.coli Expression Vector pDB525 The tac expression vector pDB525 was derived from pKK223-3 (Pharmacia).
The 3.28 kb Sphl-Sca I fragment of pKK223-3 was replaced with the equivalent fragment from pAT153 (Twigg, A.J. and Sherratt, D.J. (1980) Nature, 283, 216-218) to render the plasmid non-mobilisable; this new vector was called PTR550. PTR550 was restricted with Eagl and a 1.7kb blunt-ended EcoRI fragment from ptac-l-Iq, encoding the laclq gene, was ligated in to give pDB525 (figure 5A).
The presence of the laclq gene (Calos, M.P. (1978) Nature, 274. 762-765) ensures tight repression of the tac promoter under non-induced conditions. (b) : Construction of the H55 Protein E.coli expression vector pDB549 (i) Construction of pTR545 The approximately 7.8kb fragment of BstEII-Bglll-cut pTRH37 (European Patent Application 0297882) was ligated with oligonucleotides 1 and 2 B3042 - 40 1) 5' GTTACCAACTACCTAGACTGGATTCGTGACAACATGCGACCGTGAGGCCT ACTAGGCCAAGCTTA 3' 2) 5’ GATCTAAGCTTGGCCTAGTAGGCCTCACGGTCGCATGTTGTCACGAATC CAGTCTAGGTAGTTG 3' to give pTR545.
This places a convenient Hindlll site 3' to the H37 10 sequence in this plasmid (figure 5A). (ii) Construction of pDB546 The 0.89 kb EcoRI-Hindlll fragment of pTR545, encoding most of the H37 B chain was inserted into the expression vector pDB525 between the EcoRI and Hindlll sites to make pDB546. (Figure 5A) (iii) Construction of pDB545 The approximately lkb Ncol-Sst I fragment from pTRH37 encoding K4PK5P and part of the B chain of t-PA was ligated into EcoRl-Sst1-cut pUC19 together with oligonucleotides 3 and 4 3) 5' ATTTCATGTCTTACCAAGCACCACCTGAGCTAACCCCTGTGGTCCAGG ACTGCTAC 3' 4) 5' CATGGTAGCAGTCCTGGACCACAGGGGTTAGCTCAGGTGGTGCTTGGT AAGACATG 3' to give pDB545 (Figure 5B>. (iv) Construction of pDB549 The approximately lkb EcoRI fragment of pDB545 was ligated into EcoRI-cut and phosphatased pDB546 to give pDB549. (Figure 5C).
B3042 - 41 LbJ: Expression of H55 protein in E.coli The H55 expression plasmid pDB549 was transformed into 5 E.coli HB101. The transformed host was grown in L-Broth at 37°C to an OD55Q of 0.8-1.0 and expression was induced with ImM IPTG (isopropyl-p-D-thiogalactopyranoside). Incubation was continued for a further 3 hours. The cells were harvested and disrupted by sonication (Heat Systems10 Ultrasonics; 50 x 50%. 5 second pulses at 70W). The insoluble fraction containing H55 protein was separated by centrifugation at ll,000g for 15 minutes at 4°C. It was subsequently renatured following the protocol described by van Kimmenade, A et al; (1988), Eur. J. Biochem.,· 173. 105-114. Analysis of this refolded material using the fibrin zymography technique (Dodd et al; (1986) Thromb. Hearn; 55 (1) 94-97) showed it to be fibrinolytically active. The apparent molecular weight observed was consisent with the predicted structure.
Example 12 Construction of pDH55U encoding SYO/Plasminogen 347-546/ur PA 137-411 (H55U) Construction of the plasmid pDH55U was accomplished by substituting a 620bp MluI-BstXI fragment from pDH55 (Example 1) (Mlul site located in RSVLTR promoter, BstXI site at nucleotide 1209 in plasminogen cDNA sequence) for the analogous MluI-BstXI fragment in pTRH25 (EP A 370 711) .
The novel hybrid was expressed using HeLa cell system (Methods). Fibrinolytically active protein, as determined by fibrin plate assay, was recovered from the HeLa cell harvest medium.
B3042 - 42 Example 13 Construction of pDH56U encoding SYQ/plasminoqen 443-546/uPA 137-411 (H56U) An approximately 5.9 kb Mlul - BspMII (Mlul in RSVLTR, BspMII at nucleotide 1060 in u-PA cDNA) fragment and a 593 bp Avail - BspMII (Avail at 1693 in plasminogen, BspMII at 1060 in u-PA) fragnent were isolated from pDH55U (Example 12). These were ligated with a 816 bp Mlul - Avail (Mlul 1C in RSVLTR, Avail at 1693 in plasminogen) fragnent from pDH56 (Exanple 4) to give pDH56U. Fibrinolytically active protein was expressed as for H55U. Example 14 Amplificiatlon of expression, of H55 in CHQ cells The transfections described in Example 7 were carried out with either 10 or 20gg of pDH16 or pDH17. All were then selected as described in Example 7 and were amplified as follows.
Those dishes transfected with 20gg plasmid DNA were grown to confluency and these cells were amplified in methotrexate as mass cultures. Twenty colonies were isolated from dishes transfected with 10gg plasmid DNA (10 for each plasmid) and these were grown to confluency in 25 cm? flasks and harvested in serum-free medium for 24hrs. Fibrinolytically active protein was detected by fibrin plate assay and the four clones with the highest activity were chosen for amplification in methotrexate.
The methotrexate concentration was initially 0.05 μΜ and was increased stepwise to 5 or 10 μΜ. At 0.1 μΜ methotrexate the best cell line, as judged by activity on a fibrin plate, was the pDH17-transfected mass culture (17MC) . At ΙμΜ methotrexate the 3 best cell lines (the pDH16-and pDH17-transfected mass cultures [16MC and 17MC] and the pDH17-transfected clone (17.1)) were sub-cloned, giving 12 sub-clones per cell line. The best cell line B3042 - 43 from these 36 sub-clones plus the 3 parental lines was sub-clone #1 isolated from 16MC i.e., 16MC.1. This subclone along with the 6 next best sub-clones, plus the 3 parental lines, were amplified to 5 μΜ and, for 16MC, to μΜ methotrexate. At these methotrexate concentrations the 2 best cell lines were the sub-clone 16MC.1 at 5μΜ and the parental mass culture 16MC at ΙΟμΜ methotrexate.
Example 15 Purification of protein H55U Approx 45ml conditioned media from HeLa cells transfected with the plasmid pDH55U (see Example 12) and harvested as described in 'General Methods' was centrifuged at 9000g for 30 min and stored at -40°C for 2 months. It was then thawed and the H55U protein isolated by chromagraphy on Benzamidine Sepharose 6B (Pharmacia). The details are as follows .
A column (i.d., 16 mm; h, 12mm) of Benzamidine Sepharose was equilibrated with PBS 'A' (Dulbecco)/0.01% Tween 80. The conditioned media was applied to the column and was washed through with equilibration buffer followed by 0.02M Tris/0.5M NaCl/0.01% Tween 80 pH 7.4. H55U was then dissociated from the matrix by washing with 0.02M Tris/0.5M NaCl/0.5M L-arginine/0.01% Tween 80 pH7.4. The chromatography was at 4°C at a flow rate of 100 cmh-1.
The eluant from the column was collected as discrete fractions. Fractions containing the protein H55U were identified using the microtitre-plate based chromogenic substrate assay (General example (ix)) except that S2444 was used instead of S2288. The most active fractions were pooled and were ultrafiltered (YM10, Amicon Ltd) to 2.0 ml (the 'product').
Assay of the product by fibrin plate assay with reference to a u-PA standard showed it contained 100 IU/ml.
B3042 - 44 Analysis by SDS PAGE followed by fibrin zymography showed a single major species at apparent Mr approx. 60,000.
Example5 Purification of protein H56U Approx 45ml conditioned media from HeLa cells transfected with the plasmid pDH56U (see Example 13) and harvested as described in 'General Methods' was centrifuged at 9000g for 30 min and stored at -40°C for 2 months. It was then thawed and the H56U protein isolated by chromatography on Benzamidine Sepharose 6B (Pharmacia). The details are as follows.
A column (i.d., 16mm; h, 15mm) of Benzamidine Sepharose was equialibrated with PBS 'A' (Dulbecco)/0.01% Tween 80. The conditional media was applied to the column and was washed through with equilibration buffer followed by 0.02M Tris/0.5M NaCl/0.01% Tween 80 pH 7.4. H56U was then dissociated from the matrix by washing with 0.02M Tris/0.5M NaCl/0.5M L-arginine/0.01% Tween 80 pH 7.4. The chromatography was at 4°C at a flow rate of 100 cm h-1. The eluant from the column was collected as discrete fractions. Fractions containing the protein H56U were identified using th microtitre-plate based chromogenic substrate assay (General example (ix)) except that S2444 was used instead of S2288. The most active fractions were pooled and were ultrafiltered (YM10, Amicon Ltd) to 2.9 ml (the 'product').
Assay of the product by fibrin plate assay with reference to a u-PA standard showed it contained 47 IU/ml. Analysis by SDS PAGE followed by fibrin zymography showed a single major species at apparent Mr approx. 45,000.
B3042 - 45 Example 17 Purification of protein H56 from Spodoptera frugiperda 21 sails 2.01 serum-free conditioned media from a culture of Sf21 cells that had been infected with recombinant Baculovirus encoding H56 (see Example 10) was clarified by filtration (Whatman No 1) and then adjusted to pH 5.0 using HCI.
H56 was purifed from the media using two chromatography columns in series .
First, the media was passed down a column (i.d., 41mm; h, 38mm) of S-Sepharose Fast Flow that had been equilibrated in 20mM succinate, lOmM EACA pH 5.0. the media was washed through with equilibration buffer followed by a gradient (in equilibration buffer) of 0 to IM NaCl. A final rinse with the IM NaCl-containing buffer was then carried out.
The eluant was fractionated and assayed using the S2288 chromogenic substrate assay. The peak H56-containing fractions were those collected during the development of the NaCl gradient and were pooled. The pH of this pool was adjusted to 7.0 using NaOH.
The pH 7.0 - adjusted pool was then chromatographed on a column (i.d., 16mm; h, 55mm) of zinc chelate Sepharose that had been equilibrated in PBS 'A' (Dulbecco)/0 . 01% Tween 80. After application of the pool from the SSepharose the zinc chelate was washed with equilibration buffer followed by 0.02M sodium phosphate/0.3M NaCl/0.01% Tween 80 pH 7.4 and then 0.02M sodium phosphate/0.3M NaCl/0.05M imidazole/0.01% Tween 80 pH 7.4.
H56-containing fractions were identified by S2288 substrate assay and were pooled. The pool had a volume of ml and contained 100,000 IU by fibrin plate assay with B3042 - 46 reference to a u-PA standard curve. This particular pool was not analysed by SDS PAGE. However, other purified H56 batches, prepared under almost identical conditions, were analysed under non-reducing conditions. On silver5 staining, a single major band at apparent Mr approximately 40,000 was evident.
Example 18 Purification of protein H55 from CHQ cells Two methods were used to purify H55 from CHO cells (see Example 14). (1) 500ml serum-free conditioned media from the CHO mass culture 17MC (amplified to lOOnM methotrexate) was applied to zinc chelate Sepharose (Vt = 9ml) and lysine Sepharose (Vt = 2ml) essentially as described (Dodd, I. fiiai (1986) FEBS Lett., 2211 13-17). The H55 protein was desorbed from the lysine - Sepharose column using a 0.5M L-arginine - containing buffer and was then concentrated by ultrafiltration using a 10,000 molecular weight cut-off membrane (Centriprep10, Amicon Ltd). The ultrafiltered retentate had a volume of 0.95 ml and contained 4,800 IU by fibrin plate assay. (2) The second purification example relates to 12 litres conditioned media from the CHO cell line 16MC.1 (Example 14).
All glassware and columns were autoclaved prior to use, all buffers were 0.2μ sterile filtered. The chromatography system used (A Waters 650 advanced protein purification system) was sanitised with 2M NaOH.500ml of lysine Sepharose Fast Flow was packed into an Amicon industrial glass 70mm column and packed at linear flow rate of 300 cm.h-^- with B3042 - 47 phosphate-buffered saline (Dulbecco A). Upon completion of packing (determined when the bed height remains constant) the column was equilibrated with a further 4 bed volumes of phosphate-buffered saline (PBS).Conditioned media (12L) that had been filtered through an 8μ polypropylene filter was then applied at a linear velocity of lOOcmh-1 · The column was then washed with PBS containing 1M 10 NaCl at a linear velocity of lOOcmh-1 for 5 bed volumes. The column was then eluted using a linear gradient of 0-10mM EACA in PBS/1M NaCl (over 6 bed volumes). Elution was monitored by following 280nm adsorption of the protein eluted by the EACA. 73mg, as determined by fibrin plate analysis, of H55 was eluted in IL of solution.
The eluted pool from above was combined with a similar run to give a solution containing 132mg of H55 in 2.5L. This material was concentrated using an Amicon 8400 stirred ultrafiltration cell containing an Amicon YM-30 ultrafiltration membrane. The ultrafiltration was performed at 50 psi using nitrogen to pressurise the system. The ultrafiltration was continued until the solution was reduced in volume to 50ml when 300ml of 0.5M arginine, 0.2M NaCl, 20mM Tris/HCl, 0.01% Tween 80pH 7.4 buffer was added. Ultrafiltration was continued until the volume was again reduced to 50ml when a further 300ml of the above buffer was added. The volume was reduced to 25ml containing 120mg of H55.
Ultrafiltration/diafiltration gave a yield of 91%.Upon completion of diafiltration the material was stored frozen at -40°C.
B3042 In the figures : - 48 - Fig. 1 Plasmid pDH55i 5 Fig. 2 Plasmid pDH55 signal = t-PA signal sequence Fig . 3 Plasmid pDH56i 10 Fig. 4 Plasmid pDH56 signal = t-PA signal sequence. Fig . 5A Construction of plasmid pDB546 15 Fig . 5B Construction of plasmid pDB545 Fig. 5C Construction of plasmid pDB549
Claims (27)
1. A hybrid plasminogen activator (PA) which comprises kringle 5 or kringles 4 and 5 of plasminogen linked to the 5 B-chain of t-PA or u-PA via an amino acid sequence comprising, respectively, the t-PA cleavage site between residues 275 and 276 and the cysteine residue 264 of t-PA or the u-PA cleavage site between residues 158 and 159 and the cysteine residue 148 of u-PA.
2. A hybrid plasminogen activator according to claim 1 of the formula: < Z 3 K 4 P >m z 4 K 5 Pz 5 Bt 15 where B fc comprises residues 276-527 of t-PA, m is 0 or 1, and KgP represent kringle domains 4 and 5 derived from plasminogen and each of Zg, Z 4 and Zg represents, as appropriate, an optional N-terminal amino acid sequence or a bond or a linking sequence of amino acids which may be 20 introduced synthetically during the preparation of the hybrid PA and/or derived from native plasminogen and/or t-PA sequences, the sequence Zg comprising at least residues cys-264 and arg-275 of t-PA. 25
3. A hybrid plasminogen activator according to claim 2 wherein m is 1, Z 4 represents the native plasminogen interdomain sequence between plasminogen kringle domains 4 and 5 and Zg has at its N-terminus the sequence [GARSYQ] or [SYQ] corresponding to the L- and S-chain forms of t-PA, and 30 comprises some or all of the native plasminogen inter-domain sequence between plasminogen kringle domains 3 and 4.
4. . A hybrid plasminogen activator according to claim 3 wherein Zg comprises plasminogen residues 347-357. B3042 - 50 5. A hybrid plasminogen activator according to claim 2 wherein m is 0 and Z 4 has at its N-terminus the sequence [GARSYQ] or [SYQ] corresponding to the L- and S-chain forms of t-PA, and comprises some or all of the native plasminogen
5. Inter-domain sequence between plasminogen kringle domains 4 and 5.
6. A hybrid plasminogen activator according to claim 5 wherein Z 4 comprises plasminogen residues 443-461.
7. A hybrid plasminogen activator according to any one of claims 2 to 6 wherein is selected from: 15 2 [AAPSTCGLRQYSQPQFR] [AAPSTCGLRQYSQPQFQ] [STCGLRQYSQPQFR] (single letter amino acid notation) 20
8. GARSYQ/plasminogen 347-541/t-PA 262-527 including one and two chain variants, gly_ 3 and ser^ variants, and mixtures thereof.
9. GARSYQ/plasminogen 443-541/t-PA 262-527 including one 25 and two chain variants, gly_ 3 and ser^ variants, and mixtures thereof.
10. GARSYQ/plasminogen 347-546/u-PA 137-411 including one and two chain variants, gly_ 3 and ser-^ variants, and 30 mixtures thereof.
11. GARSYQ/plasminogen 443-546/u-PA 137-411 including one and two chain variants, gly_ 3 and ser 7 variants, and mixtures thereof. B3042 - 51
12. A hybrid plasminogen activator according to any one of claims 1 to 11 expressed using Chinese hamster ovary, HeLa, E.coli or Spodoptera frugiperda cells. 5
13. A hybrid plasminogen activator according to any preceding claim where the catalytic site essential for fibrinolytic activity is blocked by a removable blocking group. 10
14. N,N-Dimethyl-4-aminobenzoyl two chain SYQ/plasminogen 347-541/t-PA 262-527.
15. 4-Anisoyl SYQ/plasminogen 347-541/t-PA 262-527. 15
16. 4-Anisoyl SYQ/plasminogen 443-541/t-PA 262-527.
17. A pharmaceutical composition comprising a hybrid plasminogen activator according to any preceding claim in combination with a pharmaceutically acceptable carrier.
18. A hybrid plasminogen activator according to any of claims 1 to 16 for use as an active therapeutic substance.
19. A hybrid plasminogen activator according to any of 25 claims 1 to 16 for use in the treatment of thrombotic diseases.
20. Use of a hybrid plasminogen activator according to any of claims 1 to 16 for the manufacture of a medicament for 30 the treatment of thrombotic diseases.
21. A method of treating thrombotic diseases which comprises administering to the sufferer an effective nontoxic amount of a hybrid plasminogen activator according to 35 any of claims 1 to 16. B3042
22. A process for preparing a hybrid plasminogen activator according to claim 1 or 13 which process comprises expressing DNA encoding said hybrid plasminogen activator in a recombinant host cell and recovering the hybrid 5 plasminogen activator product, and thereafter optionally blocking the catalytic site essential for fibrinolytic activity with a removable blocking group.
23. A hybrid plasminogen activator according to claim 1, substantially as hereinbefore described and exemplified.
24. A process for preparing a hybrid plasminogen activator according to claim 1, substantially as hereinbefore described and exemplified.
25. A hybrid plasminogen activator according to claim 1, whenever prepared by a process claimed in claim 22 or 24.
26. A pharmaceutical composition according to claim 17, substantially as hereinbefore described.
27. Use according to claim 20, substantially as hereinbefore described.
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US5801146A (en) * | 1996-05-03 | 1998-09-01 | Abbott Laboratories | Compound and method for inhibiting angiogenesis |
US6699838B1 (en) | 1996-05-03 | 2004-03-02 | Abbott Laboratories | Antiangiogenic peptides |
CA2353699A1 (en) * | 1998-12-02 | 2000-06-08 | Oklahoma Medical Research Foundation | Human plasminogen activator |
AU784630B2 (en) * | 1999-12-06 | 2006-05-18 | Hopital Sainte-Justine | Compositions for treating abnormalities in glomerular filtration, patent ductus arteriosus and osteoporosis |
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WO1988005081A2 (en) * | 1986-12-30 | 1988-07-14 | Cetus Corporation | Novel plasminogen activator |
GB8705377D0 (en) * | 1987-03-07 | 1987-04-08 | Beecham Group Plc | Compounds |
GB8711957D0 (en) * | 1987-05-20 | 1987-06-24 | Beecham Group Plc | Compounds |
DE3851259T2 (en) * | 1987-06-04 | 1994-12-15 | Eisai Co Ltd | Mutated t-PA with Kringle replacement. |
DK359888A (en) * | 1987-07-01 | 1989-01-02 | Beecham Group Plc | FIBRINOLYTIC ENZYM |
AU3410289A (en) * | 1988-04-22 | 1989-11-24 | Collaborative Research Inc. | Plasminogen activators with increased fibrin selectivity |
GB8901422D0 (en) * | 1989-01-23 | 1989-03-15 | Fujisawa Pharmaceutical Co | New tissue plasminogen activator |
DE3903581A1 (en) * | 1989-02-07 | 1990-08-16 | Boehringer Mannheim Gmbh | FABRIC PLASMINOGEN ACTIVATOR DERIVATIVE |
EP0397366A1 (en) * | 1989-05-09 | 1990-11-14 | The Board Of Regents Of The University Of Oklahoma | Hybrid streptokinases with fibrin binding domains and methods for the synthesis of same |
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