IE42206B1 - Optical resolution of 1-t-butylamino -2,3-dihydroxypropane - Google Patents

Abstract

1470030 Optical resolution of 1-t-butylamino- 2,3-dihydroxypropane MERCK & CO Inc 10 Dec 1975 [13 Dec 1974 25 Sept 1975] 50632/75 Heading C2C Mixtures of enantiomers of 1-t-butylamino- 2,3-dihydroxypropane are optically resolved by (1) treating a solution of the mixture with a resolving agent selected from S-pyroglutamic acid, R-pyroglutamic acid, L-tartaric acid and D-tartaric acid (2) separating from the solution the solid diastereoisomer which forms and (3) recovering from the diastereoisomer a single enantiomer of 1-t-butylamino-2,3-dihydroxypropane, e.g. by treating it with a base or running a solution of the diastereoisomer through an ion-exchange resin. The initial mixture of enantiomers of 1-t-butylamino-2,3-dihydroxypropane may be made by reacting t-butylamine with glycidol.

Description

The present invention concerns the resolution of enantiomers of 1 - t- butylamino - 2,3 - dihydroxypropane from solution.

The sinister (S) enantiomer of 1 - t - butylamino - 2 ,3dihydroxypropane is especially useful in preparing the more active S - isomer of the 3 - substituted - 4 - (3 - t - butylamino - 2hydroxypropoxy) - 1,2,5 - thiadiazole class of β - adrenergic blocking agents. These β - blocking agents and methods for their preparation are disclosed in U.S. 3,657,237 and U.S. 3,781,284.

A method for preparing the £3 - enantiomer of 1 - t - butylamino10 2,3 - dihydroxypropane, as disclosed in U.S. 3,657,237, is by the reductive alkylation of a single enantiomer reactant namely Dglyceraldehyde or isopropylidene - D - glyceraldehyde. While this method can be suitably used, it requires the use of large quantities of zinc chloride and lead tetraacetate. This results in waste streams containing amounts of zinc and lead cations, which are objectionable from an ecological standpoint. Removal of these cations from waste streams is very difficult and expensive.

The present invention is based on the discovery of an ί improved process for obtaining the enantiomers of 1 - t - butyl2 amino - 2,3 - dihydroxypropane from solution using a pyroglutamic acid or a tartaric acid as resolving agent. The process does not result in any waste stream creating ecological problems.

In accordance with the present invention, there is provided a process for resolving mixtures of enantiomers of 1 - t - butylamino - 2,3 - dihydroxypropane which comprises treating a solution of said mixture in a solvent with )3 - pyroglutamic acid, Rpyroglutamic acid, L - (+) - tartaric acid or D - (—) - tartaric acid, separating, from the solution, solid diastereoisomer which forms, and recovering from the diastereoisomer a single enantiomer of 1 - t - butyl - amino - 2,3 - dihydroxypropane.

The symbols S and R designate the sinister (S) and rectus (R) isomer configurations of enantiomers. These designations refer to absolute spatial configurations in the molecule. The pairs of symbols L and D, and (—) and (+) may also be used to identify the different optical isomers. Combinations of the various symbols and designations may also be used to identify optically active isomers.

Useful organic solvents include di - (C1_4 alkyl)ketones such as methylethylketone, diisobutylketone and methylisobutylketone, C3—C5 alkanols such as amylalcohol and isobutanol and C|—C^ alkyl esters of —C^ monoalkanoic acids such as ethyl propionate, methylbutyrate and tert-butyl acetate. Small amounts of water may be admixed with these organic solvents.

When S - pyroglutamic acid is used as the resolving agent, the preferred solvents are acetone, isopropanol or mixtures of acetone or isopropanol with water. The solid diastereoisomer which separates from this resolving agent/solvent system contains the S - isomer form of the 1 - t - butylamino - 2,3 - dihydroxvpropane as S - pyroglutamic acid S - 1 - t-butylamino - 2,33 dihydroxypropane. When R - pyroglutamic acid is the resolving agent, the diastereoisomer obtained is 8 - pyroglutamic acid.

R - 1 - t - butylamino - 2,3 - dihydroxypropane.

When D (-)-or L (+) - tartaric acid is the resolving agent, again the preferred solvents used are acetone, isopropanol or isopropanol/water mixtures. A particularly suitable solvent for this system is isopropanol containing water, and preferably about 10% by weight of H20. In carrying out the resolution with the L (+) - tartaric acid, the diastereoisomer which separates contains the R - form of the 1 - t - butylamino - 2,3 - dihydroxypropane as L (+) - tartatic acid. R - 1 - t - butylamino - 2,3 - dihydroxypropane salt—with the D (-) - acid, the diastereoisomer which separates contains the S - form of the 1 - t - butylamino2,3 - dihydroxypropane as D (-) — tartaric acid S - 1 - t - butylamino - 2,3 - dihydroxypropane salt.

The resolution process may be carried out at any suitable temperature. The resolution is generally accomplished at room temperature, although higher or lower temperatures may be used.

If desired, the mixture of enantiomers and the resolving agent can be refluxed to insure complete solution and proper contact of the enantiomers and resolving agent. The refluxed solution is then cooled to room temperature or lower, generally with agitation, whereupon the diastereoisomer separates.

The present process is carried out at atmospheric pressure. Superatmospheric pressure is not required.

The amount of resolving agent used may be varied. Generally, from 0.5 to 1 mole of resolving agent is used per mole of enantiomer mixture. Molar ratios of resolving agent: enantiomer of 0.5:1 or 1:1 are particularly useful.

The single enantiomer of 1 - t - butylamino - 2,3 - dihydroxy4 propane is recovered from the separated diastereoisomer by conventional techniques. For example, the S - pyroglutamic acid St - butylamino - 2,3 - dihydroxypropane diastereoisomer can be treated with a suitable base whereby the S - 1 - t - butylamino2,3 - dihydroxypropane is freed from the - pyroglutamic acid. The S - 1 - t - butylamino - 2,3 - dihydroxypropane can then be recovered by extraction with a suitable solvent and the solvent stripped to yield the desired S - 1 - t - butylamino - 2,3 - dihydroxypropane. The neutralized S - pyroglutamic acid can be conventionally recovered from the remaining solution for re-use as a resolving agent.

Another procedure for recovering the amine enantiomer from the separated diastereoisomer is to run a solution of the diastereoisomer through a suitable ion exchange resin column and then elute the free 1 - t - butylamino - 2,3 - dihydroxypropane enantiomer. ___j The mixture of enantiomers, which can be resolved by the present process contain S and R enantiomers of 1 - t - butylamino2,3 - dihydroxypropane. These mixtures include (R, S) racemic mixtures or modifications as well as mixtures rich in R or S enantiomer.

The resolution process is relatively simple. It involves preparing a solution of the mixture of enantiomers of 1 - tbutylamino - 2,3 - dihydroxypropane in one of the solvents described above. The concentration of the enantiomer mixture in the solution can be varied. The resolving agent is then added either directly or as a solution in one of the aforesaid solvents. After the solid diastereoisomer drops out of the solution, it is separated from the solution by any convenient means e.g. by filtration, by centrifugation. This solid diastereoisomer is then treated by conventional techniques to recover the single enantiomer of 1 - tbutylamino - 2,3 - dihydroxypropane. The remaining solution, which is rich in the diastereoisomer containing the other enantiomer of 1 - t - butylamino - 2,3 - dihydroxypropane, can also be treated to recover this other enantiomer.

As pointed out above, the enantiomers of 1 - t - butylamino2,3 - dihydroxypropane are useful in preparing β - adrenergic blocking agents, such as those described in U.S. 3,657,237. The S - enantiomer of 1 - t - butylamino - 2,3 - dihydroxypropane is especially useful for preparing the more active S - isomer of the U.S. 3,657,237 ¢3 - adrenergic blocking agents.

Following are examples which illustrate the resolution process of the present invention.

EXAMPLE 1 A. Preparation of R,S - 1 - t - butylamino - 2,3 - dihydroxypropane A solution of R,:S - glycidol (105 g; 1.42 moles) in 100 ml of isopropanol was added dropwise over one hour to a solution of t - butylamine (197 g; 2.7 moles) in 200 ml isopropanol while maintaining the temperature between 46°—70°C. The solution was aged at 70°G. for one hour and the excess t - butylamine was recovered by atmospheric distillation. The distillation was continued until the pot temperature reached 110°C. Acetone (700 ml) was then added to the residue and the temperature of the final solution was adjusted to 40°—45°C. The yield of R,S - tbutylamino - 2,3 - dihydroxypropane (R,S - glycolamine) was 88%.

B. Resolution of R,S - 1 - t - butylamino - 2,3 - dihydroxypropane To the final solution from (A) was added 83.0 g (0.645 mole) of S - pyroglutamic acid (97% pure) and the resultant solution mixture was refluxed, with stirring, for 1.5 hours. This solution was then cooled to room temperature over 2.5 hours, with stirring.

The S - pyroglutamic acid S - 1 - t - butylamino - 2,3dihydroxypropane diastereoisomer, which separated from the solution, was filtered off and washed with 2X50 ml of acetone. The yield of pure diastereoisomer was 130 g (33.5% based on the R,£! - glycidol).

C. Regeneration of S - 1 - t - butylamine - 2,3 - dihydroxypropane.

The S - 1 - t - butylamino - 2,3 - dihydroxypropane was regenerated from the (B) diastereoisomer by dissolving the diastereoisomer in 200 ml of water and passing the solution through a column of 350 ml of the strongly acidic, cation-exchange resin marketed by Rohm & Hass Company under the trade mark 4.

Amberlite IR-120 (H ). The column was washed with water until a negative test for pyroglutamic acid was obtained. S - pyroglutamic acid was recovered, in excess of 95% yield, by concentrating to dryness, slurrying the residue with isopropanol and filtering off the ES - pyroglutamic acid.

The S - 1 -t - butylamino - 2,3 - dihydroxypropane was eluted from the resin by washing with 5% ammonium hydroxide solution.

The eluate was concentrated to dryness and the residue recrystallized from 150 ml of xylene to give 66.0 g of pure S - 1 - tbutylamino - 2,3 - dihydroxypropane. (31.7% yield based on the weight of R,S - glycidol).

EXAMPLE 2 R,S - t - butylamino - 2,3 - dihydroxypropane (5.88 g) and S - pyroglutamic acid (2.70 g) were mixed in isopropanol (20 ml) and heated on a steam bath until solution was complete. The solution was cooled to 50°C and seeded with the pure £3 - pyroglutamic acid S - 1 - t - butylamino - 2,3 - dihydroxypropane salt. The mixture was then allowed to cool slowly to room temperature with stirring over about a two hour period. The slurry was cooled at 0.5°C for one hour and filtered to give 4.03 g (73%) of the S- 7 pyroglutamic acid S - 1 - t - butylamino - 2,3 - dihydroxypropane diastereoisomer, which had a melting point of 140°—143°C; Z“_/D= (-) 21.9° (CHqOH). Recrystallization from 3.5 volumes of boiling isopropanol gave a 91.5¾ yield of the pure diastereoisomer melting at 143°—146°; Z_«7D= -23.4 (C=2 in CH-jOH) .

Pure S - 1 - t - butylamino - 2,3 - dihydroxypropane was recovered from the pure diastereoisomer by dissolving the diaster^oisomer in excess 50% aqueous NaOH solution and extracting the S1 - t - butylamino - 2,3 - dihydroxypropane with ether. The ethereal extract was dried over magnesium sulfate and filtered.

The product obtained was pure S - 1 - t - butylamino - 2,3 - dihydroxypropane characterized by a melting point of 83—85°C and Z«_7D= (-)30° (IN HCl).

Alternatively, the S - 1 - t - butylamino - 2,3 - dihydroxypropane was recovered by dissolving the diastereoisomer in 10 ml of water and using the ion-exchange resin (Amberlite IR-120) procedure of Example 1(C).

EXAMPLE 3 A mixture of L (+) - tartaric acid (35.0 g) and R,£3 - tbutylamino - 2,3 - dihydroxypropane (34.4 g) was dissolved in 500 ml of hot 90% isopropanol/10% water (by weight). The solution was slowly cooled to room temperature over four hours with stirring. The L (+) - tartaric acid R - 1 - t - butylamino - 2,3dihydroxypropane diastereoisomer which separated was filtered.

The yield was 45.6 g of the diastereoisomer having a melting point of 85°C and Z ®/D= +9.5°. Two additional recrystallizations from aqueous isopropanol gave substantially pure L (+) - tartaric acid R - 1 - t - butylamino - 2,3 - dihydroxypropane diastereoisomer, which had a melting point of 94°—96°C and Z«_7D= +19.9°, (C=2) in IN HCl). - 8 42206 The pure R - 1 - t - butylamino - 2,3 - dihydroxypropane was recovered from the diastereoisomer by substantially the same procedure as described in Examples 1 and 2.

Claims (5)

1. Process for resolving mixture of enantiomers of 1 - tbutylamino - 2,3 - dihydroxypropane which comprises treating a solution of said mixture in a solvent with £ - pyroglutamic acid, R - pyroglutamic acid, L - (+) - tartaric acid or D - (-) tartaric acid, separating,from the solution, solid diastereoisomer which forms, and recovering from the diastereoisomer a single enantiomer of 1 - t - butylamino - 2,3 - dihydroxypropane.

2. A process as claimed in Claim 1, in which the solvent is acetone, isopropanol or an isopropanol/water mixture.

3. A process as claimed in Claim 1 or 2, in which L - (+)tartaric acid is used and the single enantiomer is the R - form.

4. A process as claimed in Claim 3, in which the solvent is isopropanol containing about 10% by weight water. 5. A process as claimed in Claim 1 or 2, in which D - (-)tartaric acid is used and the single enantiomer is the S - form. 6. A process as claimed in Claim 5, in which the solvent is isopropanol containing about 10% by weight water. 7. A process as claimed in Claim 1 or 2, in which Spyroglutamic is used and the single enantiomer is the S - form. 8. A process as claimed in Claim 7, in which the solvent is acetone. 9. A process as claimed in Claim 7, in which the solvent is isopropanol. 10. A process as claimed in Claim 1, substantially as hereinbefore described in any one of the Examples. 11. R - pyroglutamic acid R - 1 - t - butylamino - 2,3dihydroxypropane. 12. S - pyroglutamic acid . S - 1 - t - butylamino - 2,3dihydroxypropane. 10 13. L (+) - tartaric acid · R - 1 - t - butylamino - 2,3dihydroxypropane. 14. D (-) - tartaric acid · S - 1 - t - butylamino - 2,3dihydroxypropane.

5. 15. An enantiomer of 1 - t - butylamino - 2,3 - dihydroxypropane and pyroglutamic or tartaric acid, when prepared by a process as claimed in any one of Claims 1 to 10.