HUP9904203A2 - Eljárás hosszú, ismétlődő DNS-szekvenciák stabil klónozására - Google Patents

Eljárás hosszú, ismétlődő DNS-szekvenciák stabil klónozására

Info

Publication number
HUP9904203A2
HUP9904203A2 HU9904203A HUP9904203A HUP9904203A2 HU P9904203 A2 HUP9904203 A2 HU P9904203A2 HU 9904203 A HU9904203 A HU 9904203A HU P9904203 A HUP9904203 A HU P9904203A HU P9904203 A2 HUP9904203 A2 HU P9904203A2
Authority
HU
Hungary
Prior art keywords
dna sequences
repeating dna
cloning large
large repeating
stably
Prior art date
Application number
HU9904203A
Other languages
English (en)
Inventor
John J. Harrington
Huntington F. Willard
Gil B. Van Bokkelen
Original Assignee
Case Western Reserve University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Case Western Reserve University filed Critical Case Western Reserve University
Publication of HUP9904203A2 publication Critical patent/HUP9904203A2/hu
Publication of HUP9904203A3 publication Critical patent/HUP9904203A3/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/20—Pseudochromosomes, minichrosomosomes
    • C12N2800/204—Pseudochromosomes, minichrosomosomes of bacterial origin, e.g. BAC

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

A találmány tárgyát amplifikált plazmidvektőrők képezik, amelyek egy,ismétlődő DNS-egységeket - előnyösen alfa-szatellit-DNS-t tartalmazó,mintegy 20 kb-nál nagyőbb hőszsúságú ismétlődő DNS-sőrőzatból állószekvenciát tartalmaznak. A találmány tárgyát képezik tővábbá avektőrral transzfőrmált gazdasejtek és eljárásők a vektőramplifikálására. A találmány szerinti megőldás alkalmas ismétlődő DNShősszú sőrőzatának (irányítőtt) klónőzására. ŕ
HU9904203A 1996-09-20 1997-09-22 Method for stably cloning large repeating dna sequences HUP9904203A3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/717,117 US5869294A (en) 1995-06-07 1996-09-20 Method for stably cloning large repeating DNA sequences

Publications (2)

Publication Number Publication Date
HUP9904203A2 true HUP9904203A2 (hu) 2000-04-28
HUP9904203A3 HUP9904203A3 (en) 2001-05-28

Family

ID=24880768

Family Applications (1)

Application Number Title Priority Date Filing Date
HU9904203A HUP9904203A3 (en) 1996-09-20 1997-09-22 Method for stably cloning large repeating dna sequences

Country Status (8)

Country Link
US (1) US5869294A (hu)
EP (1) EP0929666A1 (hu)
JP (1) JP2001500740A (hu)
AU (1) AU743150B2 (hu)
CA (1) CA2266426A1 (hu)
HU (1) HUP9904203A3 (hu)
NZ (1) NZ334808A (hu)
WO (1) WO1998012316A1 (hu)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6348353B1 (en) * 1995-06-07 2002-02-19 Case Western Reserve University Artificial mammalian chromosome
US20030033617A1 (en) * 1996-04-10 2003-02-13 Gyula Hadlaczky Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
AU741864B2 (en) 1996-08-26 2001-12-13 Masashi Ikeno Mammalian artificial chromosomes
US7119250B2 (en) * 1997-06-03 2006-10-10 The University Of Chicago Plant centromere compositions
US7235716B2 (en) 1997-06-03 2007-06-26 Chromatin, Inc. Plant centromere compositions
US7193128B2 (en) * 1997-06-03 2007-03-20 Chromatin, Inc. Methods for generating or increasing revenues from crops
US7227057B2 (en) 1997-06-03 2007-06-05 Chromatin, Inc. Plant centromere compositions
US6972197B1 (en) 1999-03-18 2005-12-06 The University Of Chicago Plant chromosome compositions and methods
US6900012B1 (en) 1997-06-03 2005-05-31 The University Of Chicago Plant artificial chromosome compositions and methods
WO1998055637A1 (en) * 1997-06-03 1998-12-10 Arch Development Corporation Plant artificial chromosome (plac) compositions and methods
GB9821193D0 (en) * 1998-09-30 1998-11-25 Medical Res Council Mammalian artificial chromosomes and uses thereof
US7989202B1 (en) 1999-03-18 2011-08-02 The University Of Chicago Plant centromere compositions
WO2002053576A1 (en) * 2001-01-05 2002-07-11 The General Hospital Corporation Viral delivery system for infectious transfer of large genomic dna inserts
US20030186390A1 (en) * 2001-03-22 2003-10-02 De Jong Gary Methods for delivering nucleic acid molecules into cells and assessment thereof
US7294511B2 (en) 2001-03-22 2007-11-13 Chromos Molecular Systems, Inc. Methods for delivering nucleic acid molecules into cells and assessment thereof
US6936469B2 (en) 2001-03-22 2005-08-30 Chromos Molecular Systems Inc. Methods for delivering nucleic acid molecules into cells and assessment thereof
AU2002303277A1 (en) * 2001-04-06 2002-10-21 Vladimir Larionov Artificial chromosomes that can shuttle between bacteria, yeast, and mammalian cells
US20050287647A9 (en) * 2001-05-30 2005-12-29 Carl Perez Plant artificial chromosomes, uses thereof and methods of preparing plant artificial chromosomes
CA2441937A1 (en) * 2001-05-30 2002-12-05 Chromos Molecular Systems, Inc. Chromosome-based platforms
AU2002332027A1 (en) * 2001-10-04 2003-04-14 The Government Of The United States Of America, As Tandem repeat markers
US20040265805A1 (en) * 2002-05-02 2004-12-30 Monsanto Technology, L.L.C. Method for cloning large DNA
WO2005010142A2 (en) * 2003-06-27 2005-02-03 Chromatin, Inc. Plant centromere compositions
US7330851B2 (en) * 2003-08-18 2008-02-12 Eaglehawk, Limited Data security through dissembly of data elements or connections between elements
WO2005083096A1 (en) * 2004-02-23 2005-09-09 Chromatin, Inc. Plants modified with mini-chromosomes
AU2006287553A1 (en) * 2005-09-08 2007-03-15 Chromatin, Inc. Plants modified with mini-chromosomes
WO2008112972A2 (en) * 2007-03-15 2008-09-18 Chromatin, Inc. Centromere sequences and minichromosomes
WO2011011693A1 (en) 2009-07-23 2011-01-27 Chromatin, Inc. Sorghum centromere sequences and minichromosomes
EP3910058A1 (en) 2016-04-12 2021-11-17 CarryGenes Bioengineering, LLC Methods for creating synthetic chromosomes having gene regulatory systems and uses thereof

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5049504A (en) * 1986-11-24 1991-09-17 Genex Corporation Bioadhesive coding sequences
US5202236A (en) * 1984-09-13 1993-04-13 Enzon Labs Inc. Method of producing bioadhesive protein
US5202256A (en) * 1984-09-13 1993-04-13 Enzon Labs, Inc. Bioadhesive precursor protein expression vectors
US5149657A (en) * 1984-09-13 1992-09-22 Enzon Labs Inc. Escherichia coli expression vector encoding bioadhesive precursor protein analogs comprising three to twenty repeats of the decapeptide (Ala-Lys-Pro-Ser-Tyr-Pro-Pro-Thr-Tyr-Lys)
US5242808A (en) * 1984-09-13 1993-09-07 Enzon, Inc. Production of bioadhesive precursor protein analogs by genetically engineered organisms
FR2593827B1 (fr) * 1986-01-31 1989-12-22 Inst Nat Sante Rech Med Fragments d'adn utilisables dans des vecteurs pour le maintien hereditaire de genes etrangers a l'etat autonome dans des animaux transgeniques, procede pour leur obtention et applications biologiques
US4889806A (en) * 1987-04-15 1989-12-26 Washington University Large DNA cloning system based on yeast artificial chromosomes
WO1991007496A1 (en) * 1989-11-09 1991-05-30 Allied-Signal Inc. Cloning and expression of genes encoding for polypeptides comprised of one or more repeating amino acid sequences
WO1992007080A1 (en) * 1990-10-17 1992-04-30 Board Of Regents, The University Of Texas System A eukaryotic episomal dna cloning and expression vector
US5288625A (en) * 1991-09-13 1994-02-22 Biologic Research Center Of The Hungarian Academy Of Sciences Mammalian artificial chromosomes
GB9410446D0 (en) * 1994-05-25 1994-07-13 Cancer Res Campaign Tech Novel chromosome fragment
US6348353B1 (en) * 1995-06-07 2002-02-19 Case Western Reserve University Artificial mammalian chromosome

Also Published As

Publication number Publication date
EP0929666A1 (en) 1999-07-21
CA2266426A1 (en) 1998-03-26
WO1998012316A1 (en) 1998-03-26
JP2001500740A (ja) 2001-01-23
AU743150B2 (en) 2002-01-17
HUP9904203A3 (en) 2001-05-28
AU4429497A (en) 1998-04-14
NZ334808A (en) 2001-06-29
US5869294A (en) 1999-02-09

Similar Documents

Publication Publication Date Title
HUP9904203A2 (hu) Eljárás hosszú, ismétlődő DNS-szekvenciák stabil klónozására
AU8067698A (en) Chimeric vectors comprising a phage packaging site and a portion derived from a genome of a eukaryotic virus
AU9338598A (en) Recombinant vector containing infectious, viral genome sequences greater than 100 kb, method for producing same and use for the mutagenesis of the viral sequences
NZ504214A (en) Recombination cloning using nucleic acids having recombination sites
WO1996014408A3 (en) Nucleotide sequence encoding the enzyme i-scei and the uses thereof
WO1999053044A3 (en) High throughput dna sequencing vector
NO983102L (no) Uttrykk og forsterkning av sekvenselementer (EASE) for eukaryote ekspresjonssystemer
CY1106216T1 (el) 5´ ESTs ΓΙΑ ΜΗ ΙΣΤΟ-ΕΙΔΙΚΕΣ ΕΚΚΡΙΤΙΚΕΣ ΠΡΩΤΕΪΝΕΣ
FI881185A (fi) Human mangan-superoxiddismutas (hmn-sod).
DK1222298T3 (da) Vektorer og fremgangsmåder til rekombinant proteinekspression
DK1792982T3 (da) Codon-optimeret phosphatase og ekspression heraf i gær
WO1999021976A3 (en) Rapid generation of stable mammalian cell lines producing high levels of recombinant proteins
NO892029L (no) Fremgangsmaate ved spesifikk genetisk manipulering av mikroorganisme.
EP1040184A4 (en) $ I HIGH EXPRESSION VECTOR (ESCHERICHIA COLI)
DK0843731T3 (da) Adenovirusvektorer til genterapi
MX9706290A (es) Secuencias amplificadoras, vectores que portan estas secuencias y sus utilizaciones en composiciones para la expresion de secuencias nucleotidicas en celulas transfectadas, aplicaciones terapeuticas y vacunales.
MX9701614A (es) Procedimiento de produccion de proteinas recombinantes, plasmidos y celulas modificadas.
MX9806041A (es) Proteina purificada sr-p70.
IL94053A0 (en) Methods and nucleic acid sequences for the expression of the cellulose synthase operon
EP0120629A3 (en) Molecular cloning with bifunctional plasmid vectors in bacillus subtilis, mutants and substantially stably transformed mutants of bacillus subtilis, and methods for utilizing the transformed mutants
DE3684017D1 (de) Vielfach amplifizierbare vektoren fuer hohe expression exogener dns.
AU5971090A (en) Metalloproteinase inhibitor sequence recombinant vector system for using same and recombinant-dna method for the manufacture of same
ES8706825A1 (es) Un procedimiento para preparar un plasmido recombinante
HUP0102697A2 (hu) Eljárás fehérjék előállítására transzformált élesztősejtekben
NZ314777A (en) Vectors containing a gene for an antisigma polypeptide with a multiple cloning site