HUP0000500A2 - An in vitro method for construction of a dna library - Google Patents
An in vitro method for construction of a dna libraryInfo
- Publication number
- HUP0000500A2 HUP0000500A2 HU0000500A HUP0000500A HUP0000500A2 HU P0000500 A2 HUP0000500 A2 HU P0000500A2 HU 0000500 A HU0000500 A HU 0000500A HU P0000500 A HUP0000500 A HU P0000500A HU P0000500 A2 HUP0000500 A2 HU P0000500A2
- Authority
- HU
- Hungary
- Prior art keywords
- iii
- extended
- primers
- products
- template
- Prior art date
Links
- 238000010276 construction Methods 0.000 title abstract 2
- 238000000338 in vitro Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 108091033319 polynucleotide Proteins 0.000 abstract 3
- 102000040430 polynucleotide Human genes 0.000 abstract 3
- 239000002157 polynucleotide Substances 0.000 abstract 3
- 108020004414 DNA Proteins 0.000 abstract 2
- 102000053602 DNA Human genes 0.000 abstract 2
- 230000015572 biosynthetic process Effects 0.000 abstract 2
- 230000001939 inductive effect Effects 0.000 abstract 2
- 238000003752 polymerase chain reaction Methods 0.000 abstract 2
- 238000003786 synthesis reaction Methods 0.000 abstract 2
- 108020004682 Single-Stranded DNA Proteins 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 238000000137 annealing Methods 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Construction of a library of recombined homologous polynucleotides (I) from different starting single- or double-stranded DNA templates (II) and primers (III), by inducing template shifts during in vitro polynucleotide synthesis with a polymerase, comprises: (A) synthesis of extended primers or polynucleotides by (a) denaturing double-stranded (II); (b) annealing (III) to single-stranded products; (c) extending the primers and (d) denaturing the products to recover extended (III); (B) inducing a template shift by (a) isolating extended (III) and repeating steps A(b)-A(d), using extended (III) as both primer and template, or (b) repeating steps A(b)-A(d); (C) stopping the process after the appropriate number of cycles of A plus B(a) and/or B(b),and (D) optionally amplifying the products by standard polymerase chain reaction (PCR), using specific primers for selective amplification of (I) of interest.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK62597 | 1997-05-30 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| HUP0000500A2 true HUP0000500A2 (en) | 2000-05-28 |
| HUP0000500A3 HUP0000500A3 (en) | 2001-09-28 |
Family
ID=8095703
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HU0000500A HUP0000500A3 (en) | 1997-05-30 | 1998-03-18 | An in vitro method for construction of a dna library |
Country Status (1)
| Country | Link |
|---|---|
| HU (1) | HUP0000500A3 (en) |
-
1998
- 1998-03-18 HU HU0000500A patent/HUP0000500A3/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| HUP0000500A3 (en) | 2001-09-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FD9A | Lapse of provisional protection due to non-payment of fees |