WO2005054505A3 - Method for detecting nucleic acid sequence variations - Google Patents

Method for detecting nucleic acid sequence variations Download PDF

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Publication number
WO2005054505A3
WO2005054505A3 PCT/GB2004/050032 GB2004050032W WO2005054505A3 WO 2005054505 A3 WO2005054505 A3 WO 2005054505A3 GB 2004050032 W GB2004050032 W GB 2004050032W WO 2005054505 A3 WO2005054505 A3 WO 2005054505A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid sequence
primer
primers
sequence variations
Prior art date
Application number
PCT/GB2004/050032
Other languages
French (fr)
Other versions
WO2005054505A2 (en
Inventor
Irene Gascon Escobar
Original Assignee
Genomica Sau
Irene Gascon Escobar
Williams Gareth Owen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genomica Sau, Irene Gascon Escobar, Williams Gareth Owen filed Critical Genomica Sau
Publication of WO2005054505A2 publication Critical patent/WO2005054505A2/en
Publication of WO2005054505A3 publication Critical patent/WO2005054505A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

An assay for the presence of nucleic acid sequence variations is provided, in particular SNPs. The method includes allowing a labelled first primer including one form of the variation to anneal to a target sequence including the variation; allowing a second primer having a binding sequence to anneal adjacent the first primer; ligating the primers; purifying ligated primers using the binding sequence; and annealing the purified primers to a nucleic acid corresponding to the target. This final step is preferably carried out in a microarray format, allowing high-throughput screening methods to be used.
PCT/GB2004/050032 2003-11-27 2004-11-29 Method for detecting nucleic acid sequence variations WO2005054505A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0327587.2 2003-11-27
GBGB0327587.2A GB0327587D0 (en) 2003-11-27 2003-11-27 Method for detecting nucleic acid sequence variations

Publications (2)

Publication Number Publication Date
WO2005054505A2 WO2005054505A2 (en) 2005-06-16
WO2005054505A3 true WO2005054505A3 (en) 2005-09-15

Family

ID=29797922

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2004/050032 WO2005054505A2 (en) 2003-11-27 2004-11-29 Method for detecting nucleic acid sequence variations

Country Status (2)

Country Link
GB (1) GB0327587D0 (en)
WO (1) WO2005054505A2 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4988617A (en) * 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
WO1995021271A1 (en) * 1994-02-07 1995-08-10 Molecular Tool, Inc. Ligase/polymerase-mediated genetic bit analysistm of single nucleotide polymorphisms and its use in genetic analysis
WO2002053778A2 (en) * 2001-01-05 2002-07-11 Genomicfx, Inc. Method for relative quantification of attached nucleic acids
US20020150921A1 (en) * 1996-02-09 2002-10-17 Francis Barany Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
WO2003033722A2 (en) * 2001-10-15 2003-04-24 Mount Sinai School Of Medicine Of New York University Nucleic acid amplification methods

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4988617A (en) * 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
WO1995021271A1 (en) * 1994-02-07 1995-08-10 Molecular Tool, Inc. Ligase/polymerase-mediated genetic bit analysistm of single nucleotide polymorphisms and its use in genetic analysis
US20020150921A1 (en) * 1996-02-09 2002-10-17 Francis Barany Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
WO2002053778A2 (en) * 2001-01-05 2002-07-11 Genomicfx, Inc. Method for relative quantification of attached nucleic acids
WO2003033722A2 (en) * 2001-10-15 2003-04-24 Mount Sinai School Of Medicine Of New York University Nucleic acid amplification methods

Also Published As

Publication number Publication date
WO2005054505A2 (en) 2005-06-16
GB0327587D0 (en) 2003-12-31

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