HU203442B - Female inbred sunflower line of dpg 35 sign - Google Patents

Abstract

FIELD OF THE INVENTION The female inbred sunflower line DPG 35 produced by crossing the HA89 / DPG10 and having a medium flowering time, a short plant height, no branching, a seedy stripe, anthocyanin discoloration of the underside of the stem, medium size, light green, lack of anthocyanin discoloration, lack of brightness, blistering missing, cross sectional concave, sharpness of high angle, high leaf leaf height relative to petiole insertion, narrow angle between petiole and stem, number of leaves medium, number of radish flowers narrow \ t medium, oblong shape, yellow color, yellow color of plate, elongated shape of elongate, lack of anthocyanin discoloration, head turned half down, medium size, convex shape, medium core color, black color, striped, stripes s not gray and location flank and side. A characteristic feature of the invention is that the shape of the leaf has a triangular heart, its wings are medium, the leaf edge is lacy, the depth of the incision is medium, the anthocyanin discoloration of the stigma is absent, the core is small and round. (Figure 1/1) EN 203 442 B Scope of the description: 8 pages (including Figure 1) -1-

Description

The present invention relates to a female inbred sunflower line DPG 35.

Helianthus annus var. macrocarpus, or sunflower, is a major crop in many countries around the world, especially in the United States. Sunflower is used for oil production, as food and for industrial purposes. Sunflower seeds are roasted and peeled and are marketed as a pleasure product. It is also used to a lesser extent as bird feed.

Over the past 15 years, hybrid sunflower has dominated the trade. As a result of the hybridization, varieties with significantly increased yields, stronger stems and roots, more uniform characteristics and improved resistance to insects and diseases have been developed.

Hybrid sunflower obtained by simple crossing can be produced by using a homozygous inbred line as the parent unit. The homozygous character can be achieved by inbreeding within the inbred line, and generally the sixth or seventh self-pollinated generation is considered to be genetically pure.

However, inbreeding for essential genetic identity leads to a reduction in resistance, yield and other plant characteristics. As a result, inbred sunflower lines cannot be grown for commercial purposes.

Although inbred lines developed through numerous breeding experiments in sunflower research cannot be commercially grown, they are of particular importance in the production of first generation (F1) hybrids, for example by hybridizing two inbred lines. By crossing two inbred lines, hybrid viability or heterosis occurs, and the hybrid plant has significantly increased yield, strong stem and root, homogeneity, and strong resistance to insects and disease. Self-pollination or cross-pollination of F1 hybrid plants produces second-generation (F ₂ ) hybrids. The characteristics of the F ₂ hybrid plant and its seeds are less favorable than those of the F1 hybrid, thus yielding lower and undesirable genetic characteristics. Due to the reduced characteristics, the seeds of the less favorable second generation F ₂ hybrids are not harvested by the growers. On the contrary, growers are expecting new hybrid seeds obtained each year by crossing selected inbred parental units capable of producing first generation (Fi) hybrid seeds.

The production and selection of inbred lines resulting in crossing F1 hybrid hybrids is a specialized and highly specialized task. The function of the breeder is not only to produce and select first-order inbred lines, but also to produce hybrid combinations suitable for the production and commercialization of F1 hybrids.

Due to the important role of inbred lineages in the production of commercial Fj sunflower hybrids, the development of inbred lineages has received special emphasis in the field of sunflower production and hybridization. Thus, it has become necessary to develop new inbred lines that produce first generation hybrid sunflower with higher yields and oil content, greater stability and greater resistance during hybridization. Another requirement is the homogeneity of the first generation hybrids, which facilitates mechanical harvesting and reduces labor requirements.

Sunflower inbreeding is made difficult by the need to develop controlled pollination lines. In commercial hybrid seed production, this means that cytoplasmic male sterility systems are used. As a result, female lines should be sterilized by incorporation of cytoplasm from Helianthus petiolaris by crossing back. Therefore, inbred lines used as male individuals should contain the genes required for rebuilding. In some cases, these genes need to be inserted back into the cross.

As a result, there are concerted efforts to develop inbred lines for the production of commercially available first generation sunflower hybrids.

It is therefore an object of the present invention to provide a new inbred sunflower line DPG 35.

SUMMARY OF THE INVENTION It is an object of the present invention to provide an inbreeding line DPG 35 which, when crossed with another inbred sunflower as a parent male or female, produces a first generation (Fi) hybrid with advantageous characteristics.

By inseminating other varieties of sunflower with the pollen of the inbred line DPG 35 of the present invention, it produces a high quality first generation (F1) sunflower hybrid.

The inbred line DPG 35 of the present invention can be used to produce a sunflower hybrid, which results in a first generation (F1) sunflower hybrid with advantageous properties such as high yield, high oil content, good stability, insect and disease resistance as a result of heterosis.

Thus, in accordance with the present invention, the inbred sunflower line DPG 35 is used which has high yields, excellent stems and excellent health.

The hybrid sunflower of the present invention is produced by crossing a first sunflower line with a second sunflower line, wherein the first sunflower line or the second sunflower line is a DPG 35 inbred sunflower line and the other line is a inbred sunflower line.

In the production of a new hybrid sunflower variety, we proceed by:

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(a) planting seeds of inbred line DPG and other sunflower lineages, one of which is used in a cytoplasmic male or other sterile condition, while the other 5 possesses the ability or genes necessary for fertility reproduction in the vicinity of pollination;

b) cultivating the plants resulting from planting until flowering;

c) cross-pollinating between plants from the inbred line DPG 35 and the other sunflower line; and

d) harvesting seeds of plants of the sterile line, wherein the other sunflower line may be a hybrid sunflower cross or an inbred sunflower line, preferably not the DPG 35 line.

The inbred line DPG 35 of the present invention, when used as a parent line, can be crossed with other sunflower lines, preferably inbred sunflower lines, to form first generation (F1) sunflower hybrids. Although the inbred line DPG 35 is generally used as a female during crossing, it can also be used as a male.

The inbred sunflower line DPG 35 is an oilseeded inbred sunflower line and its development is described below

Line DPG 35 is derived from the HA89 / DPG10 crossing. HA89 is the B line of the USDA. DPGlO was developed by Dekalb Argentina in Argentina and is an elongated, striped-seeded inbred line.

The HA89 / DPG10 crossing was performed in Bragado, Argentina. The following breeding procedure is summarized in the table below:

Number of rows Geographical location Breeding operation Generation 3 Redwood Falls, MN, USA self-pollination SALT 50 Bragado, Argentina self-pollination SKI 1 Glyndon, MN, USA self-pollination S2 1 Bragado, Argentina self-sterilization S3 2 Glyndon, MN, USA self-backcrossing S4 BC1 2 Bragado, Argentina self-backcrossing S5-BC2 4 Glyndon, MN, USA self-crossing + test crossing S6-BC3 4 Bragado, Argentina self-backcrossing S7-BC4 6 Glyndon, MN, USA self-crossing + test crossing S8-BC5 10 Bragado, Argentina self-backcrossing S9-BC6

Generation S2 comes from a single head of a self-pollinated plant of the previous generation. The seeds of the selected plant were planted in a row. Individual plants of the S3 generation were self-pollinated and sterilized. Sterile 40 is derived from H. petiolaris cytoplasm (Leclerq) and is followed by six generations. For the S9-BC6 generation, the sterile line is uniform and can be used for foundation purposes.

DPG 35 inbred sunflower is uniform for all 45 tested properties.

DPG 35 inbred sunflower can be considered stable in the S9-BC6 generation, so we harvest the seeds of all plants. The cultured line is phenotypically and genetically stable. 50

The inbred line DPG 35 is the property of DEKALBPFIZER GENETICS and was not accessible to users.

The seeds of the inbred line developed during the described inbreds were deposited with the General Secretariat of the Commission for the Protection of New Varieties (17, Avenue de tousville, 75007 Paris, France) under the name SL2 of the French Ministry of Agriculture.

Cells of plants of the inbred line DPG 35 according to the invention, which can be cultured in a suitable culture, then separated and regenerated to produce plants of the inbred line. The details of the generation stream N.P. Everett, K.E.P. Robinson and D. Mascarenhas, Genetics engineering of sunflower (Helianthus annus L.), Biotechnology 5: 1201-1204 (1987) and other publications dealing with the regeneration of sunflower plants from tissue culture.

As mentioned above, the DPG 35 inbred line is an oil seed sunflower that is preferably adapted for growing in most sunflower zones on earth.

This inbred line has the following characteristics (UPOV: Guidelines for Conduct of Tests for Distinctness, Homogeneity and Stability for Sunflower, 04.10.1983);

Medium of leaf, lacy

Flowering time: medium, 69 days (1 day after HA89 and 5 days after HA300)

Plant, natural height (at maturity): very short, 90 cm (10 cm lower than HA89 and HA300)

Plant, branch (at maturity): none

Seed, stripes: yes

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Stem below stalk, anthocyanin coloration (cotyledon): none or very weak

Leaf, size: medium Leaf, shape: heart-shaped Leaf, color: light green

Leaf, anthocyanin coloration (on the edge of young leaves, before flowering): none

Leaf, brightness: none

Leaf, blistering: no or very weak Leaf, lacy flesh: medium Leaf, regular lace: regular Leaf, cross-sectional shape: concave Leaf, wings: are

Leaf, vein angle: acute

Leaf, height of top of leaf blade relative to leaf stalk (2/3 of plant height): high

Angle between leaf, lower part of stem and stem: narrow

Stems, number of leaves on the main stem: medium Flowering time: medium Radius flower, number: medium Radius flower, shape: elongated radius flower, color: yellow Plum flower, color: yellow

Plum blossom, stigma anthocyanin coloration: none or very weak

Leaf, shape: oblong. Leaf, anthocyanin coloration: none

Head, Appearance (at maturity): Half-turned down Head, Size (at maturity): Medium Head, Shape of the nucleus (at maturity): Convex Plant, Natural height (at maturity): Very short

Plant, branch (at maturity): no Seed, size: medium Seed, shape: oval wide Seed, thickness: medium

Core, main color: black Core, speckled: none Core, stripes: available Core, stripes color: gray Core, stripes location: border and side.

The closest known sunflower line is HA89. The DPG 35 inbred line is statistically significantly different from the known HA89 inbred line in leaf shape (triangular heart or heart shape), leaf wing size (medium or 20 ve small), leaf blade type (lacy or fine toothed), and depth of incision (medium to fine tooth).

Other distinctive features are found in the size of the core (small or medium) and the shape of the core (round or oval).

The above characteristics of the DPG 35 inbred line relative to the known HA89 inbred line are summarized in Table 1:

Table 1

Letter- form Letter- wing Letter- wind notch depth Stigma anthocyanin coloration Grain Size Shape Mag HA89 Heartshaped small gently gently no medium oval toothed toothed I ask DPG 35 triangle- medium lacy medium no small I ask

drop heart shape

The inbred line DPG 35 is preferably used as a parent line for the production of hybrid sunflower, especially first generation (F1) hybrid sunflower. When used, this inbred line is used in combination with another inbred line and the two lines are cross-hybridized with cytoplasmic male sterile technique. That is, two inbred lines, one of which is the DPG 35 inbred line, are planted in close proximity to each other for pollination. This can be done by placing the parent lines in alternating rows, blocks, or any other way. The plants of the two lines are grown until flowering. During the period of development, the plants are preferably thinned in a six-leaf condition and regularly treated with fertilizers and / or other agrochemicals for proper development.

During flowering, when the inbred line DPG 35 is used as a male cytoplasmic male sterile in its inbred line and the other lineage is used as a male germ line, cross-hybridization is carried out using insects to transport the pollen.

Female births can also be chemically sterilized. The lines are continued to grow, while the sunflower usually cross-pollinates under the influence of wind and insects. Due to the cytoplasmic neutering of the female lineage, pollen from all the male lineages is suitable for pollination, since the pollen-bearing portions of all inbred lines that would have played a female role in hybridization have been genetically or chemically sterilized. Hybridization-41

In the course of this procedure, the parental units are reared separately from sunflower so as to avoid the undesirable introduction of pollen from foreign varieties. The necessary isolation techniques are well known to those skilled in the art.

Thereafter, both parent lines are further cultivated until maturity, but only heads from female parental units are harvested to collect the sunflower seeds of the new F1 hybrid. If necessary, seeds from a male vineyard can be harvested and used, but these seeds are not suitable as hybrid seeds.

The resulting new Fi hybrid seeds can be planted next season, when high yielding Fi hybrid sunflower plants will develop 15

The inbred line DPG 35 is suitable for the production of novel sunflower plants F1, which exhibit advantageous properties, for example, the inbred line DPG 122 is used as the female parent line and the DPG 35 inbred line is used as the male parent line. The first generation (Fi) hybrid received the DKS39 mark.

The characteristics of the first generation (Fi) G-100 hybrid were compared with those of the commercially available commercial DKS39 variant 10 (Dekalb-Pfizer Genetics). Both variants have similar yields and are particularly suitable for comparison. The results are shown in Table 2.

Hybrid Tennés (Kg / ha) test Weight (Kg / hl) humidity (%) oil content (Wt%) plant height (Cm) DKS39 1414 36.3 14.7 41.1 180.0 DKS37 1370 37.7 12.8 43.5 190.0 ** ♦ # ** **

** - 5% statistically significant

It can be seen from the foregoing that the inbred line DPG 35 provides a preferred breeding strain when used as a male or female lineage for the production of first generation hybrids with particular and advantageous properties.

Although the invention has been described in detail and in all respects, it will be apparent to those skilled in the art that various changes and modifications are possible without departing from the spirit and scope of the invention.

Claims (1)

Female inbred sunflower line DPG 35, produced by crossing HA89 / DPG10, with a medium flowering time, short plant height, no branching, deficient anthocyanin coloration of the stalk, medium size, light green, lack of anthocyanin coloration, lack of luster, lack of blistering, concave cross-section, sharp angular vein, high leaf apex height relative to apex, narrow angle of apex, medium number of leaves, radius oblong, yellow in color, plate flower yellow, peduncle oblong, lacking anthocyanin color, head turned halfway down, medium in size, convex on seed side, medium in color, main color black, striped, gray and Placem it is edging and lateral, characterized in that the shape of the leaf is triangular in heart shape, its wings are medium, the leaf edge is lacy, the depth of the incision is medium, the anthocyanin coloration of the stigma is absent and the nucleus is small and round.