HU202729B - Method for drink-feeding calves by utilizing the milk of garget cows - Google Patents

Abstract

Liquid milk from cows suffering from garget may be fed to calves provided the milk is sterilised. 0.8-2.5 vol. % pref. - 1.0-1.5 vol. % of a sterilising liquid is added to milk at 22 pref. 15-20 deg.C. Thi liquid contains 8-30 pref. - 10-15 wt. % formaldehyde, 15-40 pref. 18-25 wt. % propionic acid and/or 10-30 wt. % formic acid and in this case 0.05-0.3 wt. % sweetening agent, mict. is held at room temp. - for 36-96 vol. % proportion to liquid calves-feed fibre processes should be carried out in stainless or new metallic containers.

Description

FIELD OF THE INVENTION The present invention relates to a method of feeding calves with milk from cows with mastitis.

It is known that mastitis, despite intense control, is still a widespread disease in cattle. Milk from infected cows should not be placed on the market and, as a result of its inappropriate use and its environmental impact, it places a heavy burden on farms. Due to its valuable materials, especially its protein content, it would be desirable to use it in calf rearing, but the associated biological hazards are not solved. Milk from mastitis cows could only be used in a properly weaned state to feed calves, but economically viable germination procedures are either not effective enough or cause intolerable damage to the milk.

It is an object of the present invention to provide a method for safely feeding calves using milk from mastitis cows. It is intended that the calves be fed with a drink which is prepared by mixing a known ratio of liquid calf feed and germinated milk from mastitis cows. To this end, the milk of mastitis cows should be germinated in a way that satisfactorily reduces its total germ count, completely eliminates certain indicator bacteria (Coli, Coliform bacteria), but at the same time avoids the loss of its valuable substances and especially its protein deposits.

It has now been found that this object can be achieved by treating milk from mastitis cows at a temperature of up to 22 ° C, preferably from 15 to 20 ° C, at a temperature of 0.8-2.5% by volume, preferably 1.0- In an amount of 1.5% by volume of 8-90% by weight of formaldehyde, preferably 1015% by weight of propionic acid and / or 15-40% by weight of propionic acid and / or 10-30% by weight of acetic acid, preferably 12-18% by weight. and / or 3-8% by weight of germicidal solution, preferably 4-5% by weight of formic acid, and optionally 0.05-0.3% by weight of sweetening agent, preferably sugar syrup, fruit syrup or molasses; the mixture is kept at room temperature for 36-96 hours, preferably 48-72 hours, and then mixed with 10-75% (v / v) liquid calf feed, known per se, with the condition that the milk treatment operations listed are in containers made of acid or non-acidic material. and means.

The present invention is based on the discovery that the bactericidal action of formaldehyde is potentiated in the presence of the listed lower alkane carboxylic acids such that the germination of the milk can be achieved by the extremely small amounts of these compounds. In this way, undesirable lowering of the pH of the milk, the precipitation of the proteins and the deterioration of the milk taste to the point of inexorability are avoided.

The process according to the invention is preferably carried out as follows.

Separately treated milk from mastitis cows is collected per milking and the collected milk is cooled to a temperature below 22 ° C, preferably to 15-20 ° C. There are several ways to do this: cool it separately with a ribbed or plate milk cooler, or let the milk pass through an ice-water milk cooler at the end of milking. In the latter case, it is necessary to thoroughly clean and disinfect the refrigerator.

The germinated solution containing formaldehyde and at least one C1-C3 alkane carboxylic acid is added to the cooled milk. The germ reduction solution is prepared by dissolving the components in distilled water. Preferably, a germination solution containing 10-15% by weight of formaldehyde and 18-25% by weight of proplonic acid, 12-18% by weight of acetic acid and / or 4-5% by weight of formic acid is used. Most preferred was the germination solution containing the formaldehyde-propionic acid-formic acid mixture. A sweetening agent such as sugar syrup, molasses or fruit syrup may be added to improve the taste. The sweetener may also be incorporated into the milk together with the germicidal solution. The germicidal solution is added to the milk in a plastic, but in any case metal-free or at least acid-proof metal container, while it is also slowly and continuously mixed with a metal-free mixer. 0.8-2.5% by volume, preferably 1.01.5% by volume of germination solution is added to the milk.

The germicidal solution satisfactorily kills undesirable microorganisms within the above-mentioned quantitative limits. The admixture ratio within these limits is determined by the requirement that the pH of the skimmed milk be greater than 5, preferably 5.0 to 5.2. At lower pHs, milk proteins can easily precipitate during subsequent operations.

The milk is then kept for 36-96 hours, preferably 48-72 hours, in a covered but not necessarily sealed container, while stirring occasionally, preferably once a day. Preferably, according to the feeding regimen of the milking and calves, the milk treated with the germinator is pumped from the mastitis cows to a so-called daily reservoir with the same amount of milk as the daily milk to reach the germination time mentioned above. Therefore, a plurality of containers, preferably five containers, are used, thereby enabling them to be regularly cleaned and disinfected.

After the said germination period, the treated milk is preferably used on the third day to feed the calves due to work organization considerations. For this purpose, the above mentioned ratio is mixed with a known liquid calf feed. Liquid calf feeds include, for example, solutions of Lactophyl (manufactured by Phylaxia), BBS (produced by Biogal) or Central-Soya (produced by Agrokomplex), but are preferably produced by the Energovit Milk Fat Supplement (manufactured by the Hajduszaji Agaricultural Alliance). The de-germinated milk is added to the calf feed prior to heating, and the mixture is slowly and gradually heated to about 38-40 ° C and used to drink the wine.

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Calves can be accustomed to drinking treated milk from the 8-day committee, ie after entering the calf rearing establishment. However, habituation must be done continuously. Therefore, during the first two days, no more than 10% of the jokes, 3-4. on day 5, a mixture containing up to 30% (v / v) milk and up to 50% (v / v) from day 5 onwards is added to the calves.

If you wish to dispense the germinated milk for a longer period of time, it is not advisable to use a higher mixing ratio in the future. The process of the present invention can be used to feed calves of 8-60 days of age.

An advantage of the invention is that it allows the use of milk from mastitis cows in calf rearing. This will help to solve one of the 15 environmental problems and to make the calf feed more economical. A further biological advantage of the invention is that antibiotic residues found in the milk of mastitis cows are degraded during germination treatment and do not burden the developing calves. Finally, it has been noted that the use of the method of the present invention has not been shown to reduce calf weight gain and increase calf general resistance. 25

The invention is illustrated by the following examples.

Example 1

300 liters of milk with a total colony count of 1,380,000, including Coli bacteria, extracted from a separate wall of mastitis, were cooled to 20 ° C. 3 liters of germicidal solution containing 10% by weight of formaldehyde, 20% by weight of propionic acid and 5% by weight of formic acid are slowly added in a plastic-lined container with stirring. Cover the flask and let the milk stand at 20 ° C while stirring every 24 hours.

After an hour, the germinated milk is mixed with 40 liters of skimmed milk (supplemented with Energovit) with 300 liters of known calf formula. The mixture was heated to 40 ° C and used for feeding calves with water.

b Repeat step (a) with the exception of 45, with a total number of 1200,000. We start with milk containing coli.

Example 2

300 liters of milk from cows with inflammation in cows, with a total colony count of 80,000 and containing Coli bacteria, were cooled to 18 ° C. 4.5 L of germinating solution containing 10% by weight of formaldehyde, 15% by weight of acetic acid and 7% by weight of formic acid are added to the cooled milk in a stainless steel vessel with continuous stirring. At the same time, 300 g of sugar syrup is added as a sweetener. Cover the container, leave the milk to stand at 18 ° C, and stir every 12 hours.

After standing for 1 hour, the resulting germinated milk is mixed with 300 liters of liquid calf feed of Example 1 and used for drinking calves.

Example 3

The procedure of Example 2 was followed except that a solution containing 20% by weight of formaldehyde, 20% by weight of acetic acid and 5% by weight of formic acid was added to the germinated cow's milk as a sweetener. The germinated milk is mixed with 500 liters of liquid calf feed.

Example 4

The procedure of Example 2 was followed except that 150,000 milk with a total colony count were germinated with a solution containing 30% formaldehyde, 20% propionic acid and 5% formic acid. The resulting germinated milk is mixed with 500 liters of liquid calf feed.

To confirm the efficacy of the germination treatment, a microbiological examination was carried out using laboratory experiments using the germinating solution of Example 1 at a ratio of 1%, as well as the results of experiments 1-4. during the field experiments. Milk samples were taken 24 and 72 hours before and after mixing the germination solution. Stem counts were tested by the usual dilution method, Drigalski's medium was used to detect Coli and Coliform bacteria, blood and saline medium were used to detect the presence of other Gram positive and Gram negative bacteria, and Tacra was tested for the presence of anaerobic bacteria.

The results of our tests are summarized in Table 1. 1-6. sample from laboratory experiments, Figures 7-11. and sample from the field experiment of Examples la., Ib., 2, 3 and 4, respectively.

Table 1

Effect of germination treatment on total milk germ count and Coliform content

The sample number Reset ősszcsíra 24 hour Coli (D) blood agar ősszcsíra 72-hour Coli (D) bloody agar ősszcsíra Coli (D) bloody agar First 200000 + + 10000 _ __ 500 - Second 280000 + 4- 9000 800 - - Third 100000 + .x 5000 - 300 - - 4th 180000 + + 10000 - 500 - - 5th 250000 + + 9000 200 - - 6th 1120000 + + 10000 - 1500 - - 7th 1380000 + + 1000 - 2000 - - 8th 1200000 + + 2000 2000 - -

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Continuation of Table 1

Effect of germination treatment on total milk germ count and Colíform content

The sample number a plate Reset 24 hour Coli (D) blood agar 72 hours total germ Coli (D) blood agar Coli (D) blood agar a plate 9th 80000 + + 3000 500 10th 80000 + + 1500 - d - 11th 150000 + + d - - 0 -

Legend: + the presence of the bacterium can be detected

- the presence of the bacterium is not detectable in D Drigalski's medium

The number of bacteria in the table is expressed per milliliter. The relatively low basal germ counts at baseline are due to the antibiotic content of the milk and the relatively cool weather. The above is also confirmed by the fact that each of our inhibitory assays were positive and 0.3-0.5 cm inhibition rings were measured.

In order to determine the operational efficiency of the method according to the invention, 30 day field experiments were carried out on groups of 8-8 calves as shown in Figure 1a. and lb. Example 4, while eight groups of calves were also treated with skimmed milk supplemented with standard liquid calf feed, i.e. Energovitt, as a control.

At the beginning and end of the experiment, the calves' body weight was measured and the total and daily average body weight gain was determined. The results are reported in Table 2, where the experimental group column is the average of the two feeding experiments of the invention.

Table 2

Results of farm calf feeding experiment

8-38 days

Experimental group control group Initial average body weight 48.0 kg 48.5 kg Average body weight After 30 days of watering 66.5 kg 63.1 kg Average daily weight gain 0.61 kg 0.48 kg

As can be seen from the table, the method of the present invention does not worsen the growth of calves, even when compared to skimmed milk with a significantly higher biological value than conventional milk replacer formulas. Thus, the operational use of the process of the invention is also advantageous in this respect.

Claims (4)

20 PATENT CLAIMS 1. A method for watering calves using calves of mastitis, characterized in that milk of mastitis cows 25 to 22 ° C, preferably 15 to 20 ° C, in a ratio of 0.8 to 2.5% by volume, preferably 8-30% by weight, preferably 10 to 15% by weight, of formaldehyde and 15-40% by weight, preferably 18-25% by weight of propionic acid and / or 10-30% by weight, 30 germicidal solutions containing preferably 12-18% by weight of acetic acid and / or 3-8% by weight, preferably 4-5% by weight of formic acid and optionally 0.05-0.3% by weight of a sweetening agent, preferably sugar syrup, fruit syrup or molasses; the mixture The mixture is kept at room temperature for 36-96 hours, preferably 48-72 hours, and then mixed with 10-75% v / v of the liquid calf feed known per se, with the proviso that the listed milk treatment operations are made from acid-free or metal-free material. in pots and utensils. 2. The method of claim 1, wherein the germinating solution is administered slowly with continuous agitation of the mastitis 45 cows milk. Method according to claim 1 or 2, characterized in that the milk mixed with the germicidal solution is kept in a covered vessel at room temperature for 36-96 hours, preferably 48-72 hours, while at least Stir every 24 hours. 4. Method according to any one of claims 1 to 4, characterized in that the calves do not exceed 10% by volume on days 1 and 2 of feeding, not more than 30% by volume on days 3 and 4 of feeding, and not more than 5 days after and after feeding. Calf feed mixed with 50% v / v of mastitis cows mixed with germinated milk