HU200666B - Method for producing produce substituting animal protein by use of agricultural and food-industrial by-product and - Google Patents

Abstract

according to the invention, the larvae of a large beetle (Tenebrio Molitor) are cultivated on corn stalks or saliva in several stages of development, and the larvae are processed into fodder, separate from the soil. Scope of the description: 4 pages without figure HU 200 666 B -1-

Description

The present invention relates to a process for the production of animal protein using agricultural and food by-products and waste, which can be used for animal feed and human nutrition.

In the cyclical process known in the art, it is considered to be economical, starting from rice straw, by adding various additives to the mixture to produce mushrooms, gilts and poultry. The procedure consists in keeping cattle on rice straw inoculated with mushrooms; the resulting manure and fallen animals are mixed with sawdust, and the mixture is cultivated with Gilista, which is fed to poultry. The resulting manure is reintroduced into the process and used for mushroom growing.

SUMMARY OF THE INVENTION It is an object of the present invention to propagate a larvae of an insect species that can be cultivated economically in temperate climatic climates on agricultural waste to produce a high animal protein feed for monogastric animals.

The invention is based on the discovery that tenebrio moiitor larvae can be cultivated relatively quickly and economically under defined additives and conditions in the main mass of larvae of tenebrio moiitor, thus providing low-cost animal protein feed.

The present invention relates to a process for the production of an animal protein using an agricultural or food by-product or waste

Large flour beetle (Tenebrio moiitor) insects are cultivated separately in needle cultivation and production process, in strain cultivation and production process, laying eggs of infants on wheat bran based on larvae predominantly chopped maize stalk at 8 to 22 'C at 14 to 18' C with a light intensity of lux / m ^{2 in the} presence of 60-80 ml water / kg of medium per week, the cultivation of the adult white-backed larvae is discontinued when the first puppet specimens appear on the surface of the medium, the larvae are fluidized or screened. in ovine cultures, high egg-laying imagoes obtained after pupation are used in the production process. The imagoes are laid on wheat bran medium 8 to 12 cm thick for laying eggs in an amount of 300-500 db / m ² . The medium is supplemented with potato or fodder beet slices dipped in a dilute aqueous solution of folic acid and yeast and pollen.

Not more than 501%, preferably 10-30% by weight, of poultry fodder and / or grape marc and / or moldy silage and / or fodder beet and / or potato and vegetable waste and / or sunflower husk and / or carcasses, cm corn particles are mixed, and larvae are plated on medium with a thickness of 20-30 cm under 2-8 hours illumination at 4-16 lux / m ² daily.

The stock is placed in a heat-insulated and heated building eL The isolation is done by baiting at the time of laying. place on toi tray

In compound monogastric stomachs, 10% by weight of feed is generally used to replace animal protein feed. In poultry and waterfowl, the animal protein component in the broiler starter feed can be completely replaced by the larval material of the invention, as well as in the rearing feed. For both foods, it is advisable to increase the amount of raw fat, that is, to increase the energy level of the diet. No larval material is used in the finishing feeds. In the case of laying hens, animal proteins can be replaced in the larvae up to 10% by weight of the feed, while in litters of water the proportion of larvae can be increased. Can be used for feeding larval material in animal, pharmacy reptiles at a rate of 1-100%.

Fertilizers and waste from larvae production are suitable for soil improvement, fertilization, or composting, so the technology does not produce any pollutants to the environment.

An advantage of the process according to the invention is that its main raw material is the abundant availability of high cellulose corn stems and sunflower stems. These agricultural by-products, when mixed with food by-products and wastes, can be more efficiently converted into protein and energy feeds than conventional processes, and the process is environmentally friendly as there is virtually no harmful emissions. The use of post-harvest maize stalks and other low-value agricultural wastes represents a major technical advance in agriculture, as no insect species other than the large flour beetle is capable of converting the by-products and wastes listed into high-value animal feed and thus replaced.

The method of the invention provides infants with beet or potato cubes containing folic acid, yeast or peptone to stimulate ovarian development, so that egg laying begins 4 to 5 days after pupation and the average number of eggs deposited per female imago increases. At higher stocking densities, imagoes lay fewer eggs and do not hatch more eggs, or part of the eggs are eaten by already hatched larvae and still living beetles, or the larvae per larvae drop.

The thickness of the medium is of great importance, the thinner medium is difficult to maintain and the thicker media are prone to mold and rot. It is also important to set the optimum temperature, because at lower temperatures, life processes are very slow, larvae are undernourished and grow slower, and at less than + 4 'C, some forms of development are already damaged. At higher temperatures around 30 ° C, the larvae 's motility is greatly increased, underdeveloped, and partially destroyed in the lower layers of the medium due to lack of oxygen.

The speed and efficiency of cultivation is influenced by the degree of shredding of corn stalk, straw or other cellulosic material. On an appropriate medium, the average weight gain of the larvae in the first month is 40-50 mg.

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Using the method of the present invention, the main biological parameters of Tenebrio molitor, as compared with the literature, are as follows:

Evolutions Period professional literature by invention by ripening nutrition a few days 3-4 days embryonic development 14 days 8-11 days lárvafejlődés & -18 months 2.5-3 months preepupal state 5-6 days 2-3 days bábnyugalom 14-21 days 4-6 days full development of a generation 12-20 months 3-3.5 months

Core and productive cultures can be housed in existing buildings or foil tents with tray systems. The preferred size of the trays is 200x100 cm. For space utilization (depending on the height of the facility) 2-4-level tray rows can be created.

Breeding rotation: 3.8 months.

There are 52,531 pieces per year produced on m ² , of which 25,266 pieces are female, after which larvae are raised up to 2,101,248 pieces, which gives a yield of 280,2 kg / year. Except for the retention of breeding material

Selling orphan 272.1 kg / year after trainable 1 m ² culture surface.

Production of orphans:

Depending on egg or larval installation, the annual rotation and larvae produced at 1 mr (kg / year) are as follows:

In the case of egg laying 4.56 x 1.92 = 8.75 kg / year

5.21 x 1.92 = 10.00 kg / year for larvae

The process is illustrated in more detail by the following examples.

Example 1:

A large flour beetle (Tenebrio moliter), a 1: 1 male and female at a density of 350 imago / m ^{2, is} planted on wheat broth medium at a density of 20-30 cm. The images are fed with beet slices dipped in a dilute solution of folic acid and yeast and pollen (pollen) every 3-4 days. The temperature is set at 20 ° C and 80% relative humidity, and the medium is illuminated for 4 hours a day at which the imagoes are introduced with a light intensity of 7-12 lux / m ² . To the wheat broth medium is added 10-20 g / kg of medium potato and AP16 mineral supplement. After 10 days, the images are removed from the medium consisting of wheat bran and beet slices previously treated with folic acid, yeast and pollen. Thus, larvae of the same age are obtained.

During the larval growth phase, a medium consisting of 3 cm corn stalks is formed at a thickness of 30 cm at a temperature of 23 ° C and 80% relative humidity. The larvae inoculated for 4 hours! Illumination is 12 lux / m ² per day. 20% by weight of the medium is premixed with grape marc. The duration of larval development is 2.5-3 months.

In the larval breeding stage, 801% chopped corn stalk medium to which 60 g / kg AP17 aqueous suspension was added was used. The temperature is 25 ° C, the relative humidity is 80%, no illumination is used. Prior to the final thawing, the medium is started with a 5-10 ppm dose of a known juvenile hormone composition, EGYT 2669 EC (Viodat), so that larval weight gain can be increased. At the end of the rearing phase, when the first puppet individuals appear at the top of the medium, the resulting larvae, 23 mm in length, are separated from the culture medium by counterflow air injection. The isolated larvae were dried by microwave heat treatment and processed as a protein.

Example 2:

For the production of stock (stock):

Example 1 except that the larvae on the medium were illuminated for 4 hours at the luminous intensity of Example 1 and at a dose of 10 to 20 g / kg medium for 5 to 6 days at 5 to 6 days in the larval culture phase. and adding a solution containing starch, thereby raising the pupae to beetles and separating the beetles required for larvae production using baits and transferring them to the laying trays.

Example 3:

No. 1 The larvae isolated in Example 1c are mixed with grain maize in a 1:10 weight ratio and ground on a hammer grinder using a 3 mm diameter sieve. The feed is fed in a dairy form

In the case of poultry feed, the results are detailed below. The larvae protein according to the invention is more suitable for replacing meat meal, fish meal or blood meal than fat meal or fish meal.

The poultry breed used in the feeding experiment was the final product of the Hunnia Hybrid broiler (from Hybro 81). The experiment was conducted at the 3-week period most sensitive to poultry. The flocks were mixed in all three experiments.

and Π. The larvae protein of the present invention was used in the feed and the control contained known fish meal.

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Result of a poultry experiment

I n. control Number of chicks 30 30 30 Death 1-21 days 2 1 2 Death% 6.6 3.3 6.6 Chickens weight at introduction (g) 30.8 31.0 30.5 Chick weight 7 days (g) 100 105 95 Chick wings Day 14 (g) 265 275 260 Chicks owl Day 21 (g) 505 520 500 Weight gain in g l.hét 69.2 74.0 64.5 2 weeks 165 170 165 3 weeks 240 245 240 Feed consumption in g / day l.hét 85 85 85 2 weeks 270 280 260 3 weeks 450 460 440

The composition of the experimental feeds was as follows:

Group I

maize 56% wheat 12% soy 47% 20% fishmeal 65% - Larva protein 7% 5022-0 / M premix 5% 100% Π. group maize 56% wheat 12% soy 47% 20% fishmeal 65% 2% Larva protein 5% 5022-0 / M premix 5% 100% control maize 56% wheat 12% soy 47% 20% fishmeal 65% 7% lárvafehérje - 5022-0 / M premix 5% 100%

From the experiment it can be stated that feed supplemented with larvae protein has at least the same effect as feed containing fishmeal.

Claims (3)

PATENT CLAIMS A process for the production of animal protein using an agricultural or food by-product or waste, characterized in that: 25 large powdery mildew insects (Tenebrio molitor) are cultivated separately in the strain culture and production process, in the strain culture and production process the egg laying of the infants is based on wheat bran, larvae are chopped in maize at a temperature of 30 ° C to 22 ° C / day. 8 hours at 4-16 lux / m ² illumination in the presence of 60-80 ml water / kg medium per week, adult protein-rich larvae were cultured at In the 35 production process, the first pupae are discontinued when the dishes appear on the surface of the medium, the larvae are separated from the medium by sieving or fluidization. while in the stock culture, the high-laying imagoes obtained after pupation are used in the ter40 breeding process. Method according to claim 1, characterized in that the imagoes are laid on a wheat broth medium of 8-12 cm thickness for laying eggs in an amount of 300-500 db / m ² and the medium is additionally folic acid. Add 45 potatoes or fodder beet slices dipped in a dilute solution of yeast and pollen. Method according to Claim 1, characterized in that the larvae are cultivated with up to 50%, preferably 10-301%, of poultry litter and / or grape marc and / or moldy silage and / or feed and / or potato and vegetable waste and / or sunflower skins and / or slaughterhouse by-products and corn stalks cut into 3-4 cm particles, At 2-8 hours and 55-100 lux / m ² illumination per day, do not consider larvae placed on the medium where the medium is 20-30 cm thick.