HRP20230560T1 - Kimerni promotor za upotrebu u metaboličkom inženjeringu - Google Patents
Kimerni promotor za upotrebu u metaboličkom inženjeringu Download PDFInfo
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- HRP20230560T1 HRP20230560T1 HRP20230560TT HRP20230560T HRP20230560T1 HR P20230560 T1 HRP20230560 T1 HR P20230560T1 HR P20230560T T HRP20230560T T HR P20230560TT HR P20230560 T HRP20230560 T HR P20230560T HR P20230560 T1 HRP20230560 T1 HR P20230560T1
- Authority
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- Croatia
- Prior art keywords
- chimeric promoter
- group
- fragment
- combinations
- recombinant dna
- Prior art date
Links
- 238000012269 metabolic engineering Methods 0.000 title 1
- 239000012634 fragment Substances 0.000 claims 9
- 108090000790 Enzymes Proteins 0.000 claims 5
- 102000004190 Enzymes Human genes 0.000 claims 5
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 4
- 108020004511 Recombinant DNA Proteins 0.000 claims 4
- 238000013518 transcription Methods 0.000 claims 4
- 230000035897 transcription Effects 0.000 claims 4
- 108010078791 Carrier Proteins Proteins 0.000 claims 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 3
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 2
- 150000001720 carbohydrates Chemical class 0.000 claims 2
- 239000013613 expression plasmid Substances 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 101150093760 AZF1 gene Proteins 0.000 claims 1
- 101100371504 Arabidopsis thaliana UBC24 gene Proteins 0.000 claims 1
- 101100316018 Arabidopsis thaliana UGE4 gene Proteins 0.000 claims 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 1
- 101150039048 GCR1 gene Proteins 0.000 claims 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 1
- 101000841411 Homo sapiens Protein ecdysoneless homolog Proteins 0.000 claims 1
- 108091034117 Oligonucleotide Proteins 0.000 claims 1
- 102100037248 Prolyl hydroxylase EGLN2 Human genes 0.000 claims 1
- 101710170760 Prolyl hydroxylase EGLN2 Proteins 0.000 claims 1
- 102100029090 Protein ecdysoneless homolog Human genes 0.000 claims 1
- 101100057317 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OLI1 gene Proteins 0.000 claims 1
- 101100324965 Saccharomyces paradoxus ATP9 gene Proteins 0.000 claims 1
- 101710172711 Structural protein Proteins 0.000 claims 1
- 101150118010 TYE7 gene Proteins 0.000 claims 1
- 108020004566 Transfer RNA Proteins 0.000 claims 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 229910052799 carbon Inorganic materials 0.000 claims 1
- 230000006652 catabolic pathway Effects 0.000 claims 1
- 239000005515 coenzyme Substances 0.000 claims 1
- 239000008103 glucose Substances 0.000 claims 1
- 239000005556 hormone Substances 0.000 claims 1
- 229940088597 hormone Drugs 0.000 claims 1
- 108020004999 messenger RNA Proteins 0.000 claims 1
- 101150063702 pho2 gene Proteins 0.000 claims 1
- 101150072884 pho4 gene Proteins 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 101150056994 reb1 gene Proteins 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Claims (8)
1. Kimerni promotor naznačen time da se sastoji od dvije ili više sekvenci oligonukleotida ili njihovih dijelova koji reguliraju transkripciju gena anaboličkog i/ili kataboličkog puta i povećavaju razinu transkripcije RNK tipa kodiranje fragmenta glasničke RNK za proteine odabrane iz skupine koja se sastoji od enzima, strukturnih proteina, koenzima, transportera, antitijela, hormona i regulatora, kao fragment regulatorne RNK, kao enzimski aktivni fragment RNK ili kao fragment prijenosne RNK, taj kimerni promotor odabran je iz skupine koja se sastoji od SEQ ID NO.2, SEQ ID NO.3 ili SEQ ID NO.1.
2. Kimerni promotor prema zahtjevu 1, naznačen time da se razina transkripcije fragmenta RNK u stanici domaćina kvasca povećava kad se rastuća stanica domaćina kvasca transformira pomoću barem jednog fragmenta rekombinantne DNK koji se sastoji od kimernog promotora iz izvora ugljika odabranog iz skupine koja se sastoji od glukoze, ksiloze, etanola i njihove kombinacije.
3. Kimerni promotor temeljem bilo kojeg od prethodnih zahtjeva, naznačen time da je enzim ugljikohidrat koji modificira enzim ili transporter.
4. Kimerni promotor temeljem bilo kojeg od prethodnih zahtjeva, naznačen time da je enzim ugljikohidrat koji modificira enzim odabran iz skupine koja se sastoji od EC 5.1.3, EC 5.3.1, EC 2.7.1, EC 2.2.1, EC 1.1.1, EC 5.3.1.4, EC 2.7.1.16, EC 5.1.3.4 i njihove kombinacije, ili transporter odabran iz skupine od TC 2.A.1.1, TC 2.A.1.2 ili njihove kombinacije.
5. Kimerni promotor temeljem bilo kojeg od prethodnih zahtjeva, naznačen time da promotor sadrži faktore vezanja transkripcije odabrane iz skupine koja se sastoji od REB1, GCR1, GCR2, PHD1, TYE7, PHO2, PHO4, AZF1 i njihove kombinacije.
6. Fragment rekombinantne DNK koji sadrži kimerni promotor iz bilo kojeg zahtjeva 1 do 5.
7. Ekspresijski plazmid koji sadrži barem jedan fragment rekombinantne DNK prema zahtjevu 6.
8. Stanica domaćina transformirana barem jednim fragmentom rekombinantne DNK prema zahtjevu 6 ili transformirana barem jednim ekspresijskim plazmidom prema zahtjevu 7.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP19154364.4A EP3690052B1 (en) | 2019-01-29 | 2019-01-29 | Chimeric promoter for use in metabolic engineering |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20230560T1 true HRP20230560T1 (hr) | 2023-08-18 |
Family
ID=65243467
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20230560TT HRP20230560T1 (hr) | 2019-01-29 | 2019-01-29 | Kimerni promotor za upotrebu u metaboličkom inženjeringu |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US20230159941A1 (hr) |
| EP (2) | EP3690052B1 (hr) |
| CN (1) | CN113302306A (hr) |
| AR (1) | AR117903A1 (hr) |
| AU (1) | AU2020213619A1 (hr) |
| BR (1) | BR112021014923A2 (hr) |
| CA (1) | CA3122190A1 (hr) |
| DK (1) | DK3690052T3 (hr) |
| EA (1) | EA202192053A1 (hr) |
| ES (1) | ES2948239T3 (hr) |
| FI (1) | FI3690052T3 (hr) |
| HR (1) | HRP20230560T1 (hr) |
| HU (1) | HUE062297T2 (hr) |
| MY (1) | MY196151A (hr) |
| PL (1) | PL3690052T3 (hr) |
| RS (1) | RS64248B1 (hr) |
| WO (1) | WO2020156794A1 (hr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023094429A1 (en) * | 2021-11-23 | 2023-06-01 | Kaesler Nutrition Gmbh | Artificial yeast promoter regions |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5646012A (en) * | 1991-04-30 | 1997-07-08 | Rhone-Poulenc Rorer S.A. | Yeast promoter and use thereof |
| FR2676070B1 (fr) * | 1991-04-30 | 1994-09-30 | Rhone Poulenc Rorer Sa | Promoteur de levure et son utilisation. |
| AU2010218139B2 (en) * | 2009-02-25 | 2012-11-01 | Merck Sharp & Dohme Corp. | Metabolic engineering of a galactose assimilation pathway in the glycoengineered yeast Pichia pastoris |
| US20130295631A1 (en) * | 2010-10-01 | 2013-11-07 | The Board Of Trustees Of The University Of Illinois | Combinatorial design of highly efficient heterologous pathways |
| CN103998460A (zh) * | 2011-10-07 | 2014-08-20 | 隆扎有限公司 | 可调控启动子 |
| JP2015503351A (ja) * | 2011-12-30 | 2015-02-02 | ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー | ブタノール生産のための遺伝的スイッチ |
| EP2993230A1 (en) * | 2014-09-02 | 2016-03-09 | Clariant Produkte (Deutschland) GmbH | Polypeptides having xylanase activity with a high conversion rate of xylose-containing polysaccharides |
| CN107074918B (zh) * | 2014-10-22 | 2021-07-30 | 布塔尔格有限公司 | Gal2转运体的变异体和其用途 |
| RS56018B1 (sr) * | 2014-11-26 | 2017-09-29 | Clariant Int Ltd | Oligonukleotidna sekvenca za upotrebu u metaboličkom inženjeringu |
| WO2016110512A1 (en) * | 2015-01-06 | 2016-07-14 | Dsm Ip Assets B.V. | A crispr-cas system for a yeast host cell |
| US9506097B1 (en) * | 2015-07-10 | 2016-11-29 | The United States Of America, As Represented By The Secretary Of Agriculture | Synthetic promoter for xylose-regulated gene expression |
| KR102253479B1 (ko) * | 2015-08-05 | 2021-05-21 | 론자 리미티드 | 프로모터 변이체 |
-
2019
- 2019-01-29 DK DK19154364.4T patent/DK3690052T3/da active
- 2019-01-29 HR HRP20230560TT patent/HRP20230560T1/hr unknown
- 2019-01-29 PL PL19154364.4T patent/PL3690052T3/pl unknown
- 2019-01-29 FI FIEP19154364.4T patent/FI3690052T3/fi active
- 2019-01-29 EP EP19154364.4A patent/EP3690052B1/en active Active
- 2019-01-29 RS RS20230432A patent/RS64248B1/sr unknown
- 2019-01-29 ES ES19154364T patent/ES2948239T3/es active Active
- 2019-01-29 HU HUE19154364A patent/HUE062297T2/hu unknown
-
2020
- 2020-01-13 BR BR112021014923-6A patent/BR112021014923A2/pt unknown
- 2020-01-13 MY MYPI2021003367A patent/MY196151A/en unknown
- 2020-01-13 EP EP20700218.9A patent/EP3918079A1/en not_active Withdrawn
- 2020-01-13 CA CA3122190A patent/CA3122190A1/en active Pending
- 2020-01-13 CN CN202080009854.XA patent/CN113302306A/zh active Pending
- 2020-01-13 WO PCT/EP2020/050726 patent/WO2020156794A1/en unknown
- 2020-01-13 EA EA202192053A patent/EA202192053A1/ru unknown
- 2020-01-13 AU AU2020213619A patent/AU2020213619A1/en not_active Abandoned
- 2020-01-13 US US17/297,727 patent/US20230159941A1/en active Pending
- 2020-01-29 AR ARP200100219A patent/AR117903A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| FI3690052T3 (fi) | 2023-06-19 |
| BR112021014923A2 (pt) | 2021-09-28 |
| RS64248B1 (sr) | 2023-06-30 |
| CN113302306A (zh) | 2021-08-24 |
| CA3122190A1 (en) | 2020-08-06 |
| ES2948239T3 (es) | 2023-09-06 |
| EP3690052A1 (en) | 2020-08-05 |
| EP3918079A1 (en) | 2021-12-08 |
| EA202192053A1 (ru) | 2021-10-20 |
| AU2020213619A1 (en) | 2021-06-17 |
| MY196151A (en) | 2023-03-17 |
| PL3690052T3 (pl) | 2023-08-14 |
| AR117903A1 (es) | 2021-09-01 |
| EP3690052B1 (en) | 2023-04-19 |
| US20230159941A1 (en) | 2023-05-25 |
| DK3690052T3 (da) | 2023-06-19 |
| WO2020156794A1 (en) | 2020-08-06 |
| HUE062297T2 (hu) | 2023-10-28 |
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