HRP20220714T1 - Stanična kultura - Google Patents

Stanična kultura Download PDF

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Publication number
HRP20220714T1
HRP20220714T1 HRP20220714TT HRP20220714T HRP20220714T1 HR P20220714 T1 HRP20220714 T1 HR P20220714T1 HR P20220714T T HRP20220714T T HR P20220714TT HR P20220714 T HRP20220714 T HR P20220714T HR P20220714 T1 HRP20220714 T1 HR P20220714T1
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HR
Croatia
Prior art keywords
chambers
fluid communication
chamber
holder
communication path
Prior art date
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HRP20220714TT
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English (en)
Inventor
Fiona Oakley
Lee BORTHWICK
Clive Griffiths
Michael Drinnan
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Fibrofind Ip Limited
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Publication of HRP20220714T1 publication Critical patent/HRP20220714T1/hr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/20—Mixing the contents of independent containers, e.g. test tubes
    • B01F31/23—Mixing the contents of independent containers, e.g. test tubes by pivoting the containers about an axis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16—Vibrating; Shaking; Tilting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/12—Pulsatile flow
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5067—Liver cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0457—Moving fluids with specific forces or mechanical means specific forces passive flow or gravitation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Claims (18)

1. Aparat za kulturu za kultivaciju stanica i/ili tkiva ili njegovog dijela i koji sadrži tijelo držača, tijelo držača sadrži više komora za medij za kulturu stanica i najmanje jedan put za fluidnu komunikaciju koji se pruža između najmanje dvije respektivne komore od više komora, pri čemu svaki put za fluidnu komunikaciju dopušta dvosmjerni protok tekućine između navedene najmanje dvije komore, i pri čemu navedeni put za fluidnu komunikaciju ima širinu od između 1.5mm i 3.5mm; i pri čemu je svaka komora od više komora konfigurirana tako da je za smještanje komorskog umetnutog elementa konfiguriranog za podržavanje potpornog elementa stanica, pri čemu navedeni komorski umetnuti element dalje sadrži stanični potporni element koji ima više pora, pri čemu navedenih više pora imaju prosječan promjer od između oko 8µm do oko 150µm.
2. Aparat prema patentnom zahtjevu 1, naznačen time, što svaka komora od navedenih više komora sadrži bazalni element i najmanje jedan bočni zidni element.
3. Aparat prema patentnom zahtjevu 2, naznačen time, što put za fluidnu komunikaciju sadrži kanal za prolaz koji se pruža između bazalnog elementa ili bočnog zidnog elementa prve od više komora do bazalnog elementa ili bočnog zidnog elementa sljedeće od više komora.
4. Aparat prema patentnom zahtjevu 2, naznačen time, što put za fluidnu komunikaciju uključuje otvor za prolaz koji se pruža između bazalnog elementa ili bočnog zidnog elementa prve od više komora do bazalnog elementa ili bočnog zidnog elementa sljedeće od više komora.
5. Aparat prema patentnom zahtjevu 3 ili patentnom zahtjevu 4, naznačen time, što se prva komora nalazi pored sljedeće komore.
6. Aparat prema bilo kojem od prethodnih patentnih zahtjeva, naznačen time, što tijelo držača sadrži više puteva za fluidnu komunikaciju, svaki put za fluidnu komunikaciju pruža se između najmanje dvije komore od više komora.
7. Aparat prema patentnom zahtjevu 6, naznačen time, što je svaki put za fluidnu komunikaciju od više puteva za fluidnu komunikaciju suštinski paralelan i na udaljenosti od svakog drugog puta za fluidnu komunikaciju.
8. Aparat prema bilo kojem od prethodnih patentnih zahtjeva, naznačen time, što svaka komora od više komora sadrži otvor za primanje i/ili uklanjanje medija za kulturu tkiva i/ili respektivnog komorskog umetnutog elementa.
9. Aparat prema bilo kojem od prethodnih patentnih zahtjeva, naznačen time, što dalje sadrži najmanje jedan obično cilindrični komorski umetnuti element koji sadrži više prirubnica koje se pružaju radijalno prema van za podupiranje komorskog umetnutog elementa u respektivnoj komori od više komora.
10. Aparat prema bilo kojem od prethodnih patentnih zahtjeva, naznačen time, što je ukupna zapremina najmanje jednog puta za fluidnu komunikaciju i najmanje dvije odgovarajuće komore između 1ml i 8ml.
11. Aparat prema patentnom zahtjevu 10, naznačen time, što je ukupna zapremina između 1.5ml i 4ml.
12. Aparat prema patentnom zahtjevu 1, naznačen time, što, veći broj pora ima prosječan promjer između oko 8μm i oko 100μm.
13. Aparat prema bilo kojem od prethodnih patentnih zahtjeva, naznačen time, što tijelo držača sadrži najmanje dvanaest komora, npr. najmanje dvadeset četiri komore.
14. Aparat prema bilo kojem od prethodnih patentnih zahtjeva, naznačen time, što tijelo držača sadrži vanjski obodni zidni element, i dalje pri čemu vanjski obodni zidni element uključuje prema unutra povučen dio konfiguriran da omogući da se više tijela držača naslaže okomito.
15. Aparat prema bilo kojem od prethodnih patentnih zahtjeva, naznačen time, što je tijelo držača sačinjeno od materijala odabranog između Zeonex™, Zeonor™, polistirena, polikarbonata, polietilena, polipropilena, PMMA, celuloza acetata i stakla.
16. Aparat prema bilo kojem od prethodnih patentnih zahtjeva, naznačen time, što je svaka respektivna komora od više komora duboka između oko 16mm i 19mm.
17. Aparat prema bilo kojem od prethodnih patentnih zahtjeva, naznačen time, što aparat za kulturu uključuje još i poklopac koji se može ukloniti, postavljen preko tijela držača, poklopac osigurava suštinski ravan pokrivač koji se pruža preko više komora.
18. Aparat prema bilo kojem od prethodnih patentnih zahtjeva, naznačen time, što je više komora raspoređeno u redovima i kolonama u respektivnom ortogonalnom odnosu unutar tijela držača.
HRP20220714TT 2015-10-22 2016-10-21 Stanična kultura HRP20220714T1 (hr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1518767.7A GB201518767D0 (en) 2015-10-22 2015-10-22 Cell culture
EP20194991.4A EP3766582B1 (en) 2015-10-22 2016-10-21 Cell culture

Publications (1)

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HRP20220714T1 true HRP20220714T1 (hr) 2022-07-22

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HRP20220714TT HRP20220714T1 (hr) 2015-10-22 2016-10-21 Stanična kultura
HRP20201713TT HRP20201713T1 (hr) 2015-10-22 2020-10-23 Stanična kultura

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Country Status (14)

Country Link
US (1) US20190002809A1 (hr)
EP (2) EP3365107B8 (hr)
CY (1) CY1123718T1 (hr)
DK (2) DK3365107T3 (hr)
ES (2) ES2827526T3 (hr)
GB (1) GB201518767D0 (hr)
HR (2) HRP20220714T1 (hr)
HU (2) HUE059470T2 (hr)
LT (2) LT3365107T (hr)
PL (2) PL3365107T3 (hr)
PT (2) PT3365107T (hr)
RS (2) RS61056B1 (hr)
SI (2) SI3365107T1 (hr)
WO (1) WO2017068376A1 (hr)

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CN107022484A (zh) * 2017-05-27 2017-08-08 余裕炉 一种胚胎培养单元及其制作方法
US11421194B2 (en) * 2018-12-06 2022-08-23 The Trustees Of The Stevens Institute Of Technology Pumpless platform for high-throughput dynamic multicellular culture and chemosensitivity evaluation
US11299700B1 (en) 2021-02-19 2022-04-12 Acequia Biotechnology, Llc Bioreactor containers and methods of growing hairy roots using the same
US20220388007A1 (en) * 2021-06-04 2022-12-08 Advanced Solutions Life Sciences, Llc Tiltable stages and methods of using the same
CN113621558A (zh) * 2021-09-08 2021-11-09 上海万何圆生物科技有限公司 一种新鲜肝组织切片的体外培养方法
CN114561338B (zh) * 2022-03-22 2024-04-19 闻庆平 一种肺气血屏障损伤模型、其建立方法及其应用

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Also Published As

Publication number Publication date
EP3766582A1 (en) 2021-01-20
LT3365107T (lt) 2020-11-25
DK3365107T3 (da) 2020-10-26
EP3365107B1 (en) 2020-10-07
GB201518767D0 (en) 2015-12-09
RS61056B1 (sr) 2020-12-31
EP3365107B8 (en) 2020-12-16
EP3766582B1 (en) 2022-05-25
ES2917874T3 (es) 2022-07-12
DK3766582T3 (da) 2022-06-20
CY1123718T1 (el) 2022-03-24
HUE059470T2 (hu) 2022-11-28
ES2827526T3 (es) 2021-05-21
EP3365107A1 (en) 2018-08-29
WO2017068376A1 (en) 2017-04-27
PL3766582T3 (pl) 2022-07-04
SI3365107T1 (sl) 2020-12-31
US20190002809A1 (en) 2019-01-03
PT3766582T (pt) 2022-06-17
RS63284B1 (sr) 2022-06-30
PT3365107T (pt) 2020-10-28
LT3766582T (lt) 2022-06-27
HRP20201713T1 (hr) 2021-04-02
PL3365107T3 (pl) 2021-03-08
HUE054259T2 (hu) 2021-08-30
SI3766582T1 (sl) 2022-07-29

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