HRP20211601T1 - Rekombinantni proteini bez fenilalanina (phe) za liječenje fenilketonurije - Google Patents

Rekombinantni proteini bez fenilalanina (phe) za liječenje fenilketonurije Download PDF

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Publication number
HRP20211601T1
HRP20211601T1 HRP20211601TT HRP20211601T HRP20211601T1 HR P20211601 T1 HRP20211601 T1 HR P20211601T1 HR P20211601T T HRP20211601T T HR P20211601TT HR P20211601 T HRP20211601 T HR P20211601T HR P20211601 T1 HRP20211601 T1 HR P20211601T1
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Croatia
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seq
sequence
protein
phenylalanine
sequences
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HRP20211601TT
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Qingshan Li
Olof KÄMPE
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Tanea Medical Ab
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Publication of HRP20211601T1 publication Critical patent/HRP20211601T1/hr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
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    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast

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  • Health & Medical Sciences (AREA)
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  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
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  • Polymers & Plastics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (11)

1. Rekombinantni protein bez fenilalanina, pri čemu se protein odabire iz sljedova koji pokazuju barem 85-postotnu identičnost sa slijedom SEQ ID BR: 12 i pri čemu su se svi fenilalanini zamijenili velikim neutralnim aminokiselinama (LNAA) koje su odabrane iz slijeda Tyr, Trp, Thr, Ile, Leu, Val, Met ili His.
2. Rekombinantni protein bez fenilalanina prema zahtjevu 1., pri čemu se protein odabire iz sljedova koji pokazuju barem 95-postotnu identičnost sa slijedom SEQ ID BR: 12 i pri čemu su se svi fenilalanini zamijenili velikim neutralnim aminokiselinama (LNAA) koje su odabrane iz slijeda Tyr, Trp, Thr, Ile, Leu, Val, Met ili His.
3. Rekombinantni protein bez fenilalanina prema bilo kojem od zahtjeva 1. – 2. koji pokazuje barem 85-postotnu identičnost sa sljedovima SEQ ID BR: 28 – 29 ili sa slijedom SEQ ID BR: 76.
4. Rekombinantni protein bez fenilalanina prema zahtjevu 3. koji pokazuje barem 95-postotnu identičnost sa sljedovima SEQ ID BR: 28 – 30 ili sa slijedom SEQ ID BR: 76.
5. Rekombinantni protein bez fenilalanina koji pokazuje 100-postotnu identičnost sa sljedovima SEQ ID BR: 28 – 30 ili sa slijedom SEQ ID BR: 76.
6. Izolirana nukleinska kiselina koja sadrži slijed nukleinske kiseline koji kodira protein kako je definirano u bilo kojem od zahtjeva 1. – 5.
7. Vektor koji sadrži slijed nukleinske kiseline koji kodira protein kako je definirano u bilo kojem od zahtjeva 1. – 5.
8. Vektor prema zahtjevu 7., pri čemu se vektor odabire iz skupine koja se sastoji od sljedova SEQ ID BR: 15 (pHT01), SEQ ID BR: 16 (pHT43), SEQ ID BR: 17 (pHT100), SEQ ID BR: 18 (pHT223), SEQ ID BR: 19 (pHT250), SEQ ID BR: 20 (pHT431), SEQ ID BR: 21 (pHT432) i SEQ ID BR: 22 (pHT433).
9. Rekombinantni mikroorganizam koji sadrži vektor iz zahtjeva 7. ili 8., pri čemu je rekombinantni mikroorganizam B. licheniformis.
10. Rekombinantni mikroorganizam prema zahtjevu 9., odnosno B. licheniformis CICC10266.
11. Medicinska hrana koja sadrži rekombinantni protein bez fenilalanina kako je definirano u bilo kojem od zahtjeva 1.–5.
HRP20211601TT 2014-09-22 2015-09-22 Rekombinantni proteini bez fenilalanina (phe) za liječenje fenilketonurije HRP20211601T1 (hr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462053433P 2014-09-22 2014-09-22
EP15767155.3A EP3197472B1 (en) 2014-09-22 2015-09-22 Recombinant phe-free proteins for use in the treatment of phenylketonuria
PCT/EP2015/071788 WO2016046234A2 (en) 2014-09-22 2015-09-22 Recombinant phe-free proteins for use in the treatment of phenylketonuria

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HRP20211601T1 true HRP20211601T1 (hr) 2022-01-21

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US (1) US10174354B2 (hr)
EP (1) EP3197472B1 (hr)
DK (1) DK3197472T3 (hr)
ES (1) ES2896401T3 (hr)
HR (1) HRP20211601T1 (hr)
LT (1) LT3197472T (hr)
PL (1) PL3197472T3 (hr)
SI (1) SI3197472T1 (hr)
WO (1) WO2016046234A2 (hr)

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WO2010045659A1 (en) 2008-10-17 2010-04-22 American Gene Technologies International Inc. Safe lentiviral vectors for targeted delivery of multiple therapeutic molecules
WO2017007994A1 (en) 2015-07-08 2017-01-12 American Gene Technologies International Inc. Hiv pre-immunization and immunotherapy
US10137144B2 (en) 2016-01-15 2018-11-27 American Gene Technologies International Inc. Methods and compositions for the activation of gamma-delta T-cells
CN108883100B (zh) 2016-01-15 2022-11-25 美国基因技术国际有限公司 用于活化γ-δT细胞的方法和组合物
JP7153332B2 (ja) 2016-02-08 2022-10-14 アメリカン ジーン テクノロジーズ インターナショナル インコーポレイテッド Ｈｉｖワクチン接種および免疫療法
EP4036231A1 (en) 2016-03-09 2022-08-03 American Gene Technologies International Inc. Combination vectors and methods for treating cancer
US11976292B2 (en) 2016-06-08 2024-05-07 American Gene Technologies International Inc. Non-integrating viral delivery system and methods related thereto
AU2017292582C1 (en) 2016-07-08 2021-11-11 American Gene Technologies International Inc. HIV pre-immunization and immunotherapy
WO2018017882A1 (en) 2016-07-21 2018-01-25 American Gene Technologies International Inc. Viral vectors for treating parkinson's disease
DK3290436T3 (da) 2016-09-01 2019-08-19 Metax Inst Fuer Diaetetik Gmbh Phenylalanin-fri protein til behandlingen af pku
WO2018187231A2 (en) * 2017-04-03 2018-10-11 American Gene Technologies International Inc. Compositions and methods for treating phenylketonuria
CN110746494B (zh) * 2019-09-27 2021-07-20 深圳市诺维健生物技术有限责任公司 一组特殊膳食蛋白质
CN110747218A (zh) * 2019-09-27 2020-02-04 深圳市诺维健生物技术有限责任公司 一种特殊膳食蛋白质的生产方法与应用
CN110846299B (zh) * 2019-11-22 2021-09-24 江南大学 一种前导肽突变体及其在角蛋白酶生产中的应用
WO2021224152A1 (en) * 2020-05-05 2021-11-11 Basf Se Improving expression in fermentation processes
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

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GB9310472D0 (en) * 1993-05-20 1993-07-07 Univ Warwick Phenylalanine-free protein and dna coding thereof
GB9314802D0 (en) * 1993-07-16 1993-08-25 Pharmaceutical Proteins Ltd Modified proteins
AU1939895A (en) * 1994-03-01 1995-09-18 E.R. Squibb & Sons, Inc. Enzymatic debridement compositions and methods
WO1996017064A1 (en) * 1994-12-02 1996-06-06 Indiana University Foundation Phenylalanine free proteins
US20110171718A1 (en) * 2009-07-31 2011-07-14 Danisco Us Inc. Proteases With Modified Pre-Pro Regions
US20150126441A1 (en) * 2012-03-26 2015-05-07 Pronutria, Inc. Nutritive Fragments and Proteins with Low or No Phenylalanine and Methods
EP2922416A4 (en) * 2012-11-20 2016-07-20 Pronutria Inc MANIPULATED SECRETATED PROTEINS AND METHOD

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SI3197472T1 (sl) 2022-01-31
LT3197472T (lt) 2021-11-10
WO2016046234A3 (en) 2016-08-11
US20180016613A1 (en) 2018-01-18
ES2896401T3 (es) 2022-02-24
US10174354B2 (en) 2019-01-08
DK3197472T3 (da) 2021-10-18
WO2016046234A2 (en) 2016-03-31
EP3197472B1 (en) 2021-08-04
PL3197472T3 (pl) 2021-12-27
EP3197472A2 (en) 2017-08-02

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