HRP20210753T1 - Polipeptid koji ima aktivnost beta-glukozidaze i njegova upotreba - Google Patents
Polipeptid koji ima aktivnost beta-glukozidaze i njegova upotreba Download PDFInfo
- Publication number
- HRP20210753T1 HRP20210753T1 HRP20210753TT HRP20210753T HRP20210753T1 HR P20210753 T1 HRP20210753 T1 HR P20210753T1 HR P20210753T T HRP20210753T T HR P20210753TT HR P20210753 T HRP20210753 T HR P20210753T HR P20210753 T1 HRP20210753 T1 HR P20210753T1
- Authority
- HR
- Croatia
- Prior art keywords
- polypeptide
- beta
- esterase
- endo
- rhamnogalacturonan
- Prior art date
Links
- 229920001184 polypeptide Polymers 0.000 title claims 8
- 102000004196 processed proteins & peptides Human genes 0.000 title claims 8
- 108090000765 processed proteins & peptides Proteins 0.000 title claims 8
- 108010047754 beta-Glucosidase Proteins 0.000 title claims 3
- 102000006995 beta-Glucosidase Human genes 0.000 title claims 3
- 230000000694 effects Effects 0.000 title claims 3
- 238000000034 method Methods 0.000 claims 9
- 102000004190 Enzymes Human genes 0.000 claims 4
- 108090000790 Enzymes Proteins 0.000 claims 4
- 229940088598 enzyme Drugs 0.000 claims 4
- 239000012978 lignocellulosic material Substances 0.000 claims 4
- 108010059820 Polygalacturonase Proteins 0.000 claims 3
- 238000002360 preparation method Methods 0.000 claims 3
- 108010059892 Cellulase Proteins 0.000 claims 2
- 102000005936 beta-Galactosidase Human genes 0.000 claims 2
- 108010005774 beta-Galactosidase Proteins 0.000 claims 2
- 108010055059 beta-Mannosidase Proteins 0.000 claims 2
- 229940106157 cellulase Drugs 0.000 claims 2
- 108010093305 exopolygalacturonase Proteins 0.000 claims 2
- 238000000227 grinding Methods 0.000 claims 2
- 229940059442 hemicellulase Drugs 0.000 claims 2
- 108010002430 hemicellulase Proteins 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
- 101000709143 Aspergillus aculeatus Rhamnogalacturonate lyase A Proteins 0.000 claims 1
- 101001065065 Aspergillus awamori Feruloyl esterase A Proteins 0.000 claims 1
- 102100032487 Beta-mannosidase Human genes 0.000 claims 1
- 108010008885 Cellulose 1,4-beta-Cellobiosidase Proteins 0.000 claims 1
- 108010001817 Endo-1,4-beta Xylanases Proteins 0.000 claims 1
- 101710111935 Endo-beta-1,4-glucanase Proteins 0.000 claims 1
- 108090000371 Esterases Proteins 0.000 claims 1
- 108090000604 Hydrolases Proteins 0.000 claims 1
- 102000004157 Hydrolases Human genes 0.000 claims 1
- 101000728666 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) Putative rhamnogalacturonase Proteins 0.000 claims 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 claims 1
- 108010044725 Pectate disaccharide-lyase Proteins 0.000 claims 1
- 108010029182 Pectin lyase Proteins 0.000 claims 1
- 108010093941 acetylxylan esterase Proteins 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 108010030291 alpha-Galactosidase Proteins 0.000 claims 1
- 102000005840 alpha-Galactosidase Human genes 0.000 claims 1
- 239000002585 base Substances 0.000 claims 1
- 230000003197 catalytic effect Effects 0.000 claims 1
- 108010080434 cephalosporin-C deacetylase Proteins 0.000 claims 1
- -1 endo-galactanase Proteins 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 108010038658 exo-1,4-beta-D-xylosidase Proteins 0.000 claims 1
- 238000004880 explosion Methods 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 108010087558 pectate lyase Proteins 0.000 claims 1
- 108010072638 pectinacetylesterase Proteins 0.000 claims 1
- 102000004251 pectinacetylesterase Human genes 0.000 claims 1
- 108020004410 pectinesterase Proteins 0.000 claims 1
- 150000002978 peroxides Chemical class 0.000 claims 1
- 108010035322 rhamnogalacturonan acetylesterase Proteins 0.000 claims 1
- 239000002904 solvent Substances 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Claims (9)
1. Postupak za proizvodnju šećera ili šećera od lignoceluloznog materijala, koji obuhvaća:
a) predobradu lignoceluloznog materijala prije enzimatske obrade izlaganjem (vrućoj) vodi, pari (eksplozija pare), kiselini, bazi, otapalu, toplini, peroksidu, ozonu, mehaničkom usitnjavanju, brušenju, mljevenju ili brzom smanjenju tlaka, kombinaciji bilo koje dvije ili više njih;
b) predsaharifikaciju lignoceluloznog materijala inkubacijom s enzimom ili smjesom enzima;
c) dovođenje u kontakt lignoceluloznog materijala s (i) polipeptidom koji sadrži aminokiselinsku sekvencu navedenu u SEQ ID NO:2 ili njegovom varijantom polipeptida, pri čemu varijanta polipeptida ima najmanje 98% istovjetnosti sekvence sa sekvencom navedenom u SEQ ID NO:2, pri čemu polipeptid ima aktivnost beta-glukozidaze i pri čemu varijanta polipeptida ima katalitički ostatak Asp277 - Glu505 (položaji kao u SEQ ID NO: 2), ili (ii) pripravkom koji sadrži polipeptid iz (i).
2. Postupak u skladu s patentnim zahtjevom 1, naznačen time što se temperatura koraka predsaharifikacije kreće u rasponu od 20°C do 85°C.
3. Postupak u skladu s patentnim zahtjevom 1 ili 2, naznačen time što se pH smjese predsaharifikacije kreće u rasponu od 3,0 do 7,0.
4. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 do 3, naznačen time što se predsaharifikacija odvija u vremenu od 2 sata do 48 sati.
5. Postupak prema bilo kojem od patentnih zahtjeva 1 do 4, naznačen time što pripravak koji sadrži polipeptid iz (i) nadalje sadrži celulazu i/ili hemicelulazu i/ili pektinazu.
6. Postupak u skladu s patentnim zahtjevom 5, naznačen time što je celulaza celobiohidrolaza, endo-beta-1,4-glukanaza, beta-glukozidaza ili beta-(1,3)(1,4)-glukanaza.
7. Postupak u skladu s patentnim zahtjevom 5 ili 6, naznačen time što je hemicelulaza endoksilanaza, β- ksilozidaza, α-L-arabionofuranozidaza, α-D-glukuronidaza, acetil-ksilan esteraza, feruloil esteraza, kumaroil esteraza, α-galaktozidaza, β-galaktozidaza, β-mananaza ili β- manozidaza.
8. Postupak prema bilo kojem od patentnih zahtjeva 5 do 7, naznačen time što je pektinaza endo poligalakturonaza, pektin metil esteraza, endo-galaktanaza, beta-galaktozidaza, pektin acetil esteraza, endo-pektin liaza, pektat liaza, alfa ramnozidaza, egzo-galakturonaza, egzo-poligalakturonat liaza, ramnogalakturonan hidrolaza, ramnogalakturonan liaza, ramnogalakturonan acetil esteraza, ramnogalakturonan galakturonohidrolaza ili ksilogalakturonaza.
9. Postupak prema bilo kojem od patentnih zahtjeva 1 do 8, naznačen time što pripravak nadalje sadrži enzim iz obitelji GH61 ili drugi enzim koji ima aktivnost pojačavanja celulaze.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US35959610P | 2010-06-29 | 2010-06-29 | |
| EP10167776 | 2010-06-29 | ||
| EP17200641.3A EP3318574B1 (en) | 2010-06-29 | 2011-06-23 | Polypeptide having beta-glucosidase activity and uses thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20210753T1 true HRP20210753T1 (hr) | 2021-06-11 |
Family
ID=76301176
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20210753TT HRP20210753T1 (hr) | 2010-06-29 | 2021-05-12 | Polipeptid koji ima aktivnost beta-glukozidaze i njegova upotreba |
Country Status (2)
| Country | Link |
|---|---|
| HR (1) | HRP20210753T1 (hr) |
| HU (1) | HUE054139T2 (hr) |
-
2011
- 2011-06-23 HU HUE17200641A patent/HUE054139T2/hu unknown
-
2021
- 2021-05-12 HR HRP20210753TT patent/HRP20210753T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| HUE054139T2 (hu) | 2021-08-30 |
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