HRP20200975T1 - Beta-glukozidaza i njezina upotreba - Google Patents
Beta-glukozidaza i njezina upotreba Download PDFInfo
- Publication number
- HRP20200975T1 HRP20200975T1 HRP20200975TT HRP20200975T HRP20200975T1 HR P20200975 T1 HRP20200975 T1 HR P20200975T1 HR P20200975T T HRP20200975T T HR P20200975TT HR P20200975 T HRP20200975 T HR P20200975T HR P20200975 T1 HRP20200975 T1 HR P20200975T1
- Authority
- HR
- Croatia
- Prior art keywords
- beta
- polypeptide
- variant polypeptide
- seq
- variant
- Prior art date
Links
- 108010047754 beta-Glucosidase Proteins 0.000 title claims 3
- 102000006995 beta-Glucosidase Human genes 0.000 title claims 3
- 229920001184 polypeptide Polymers 0.000 claims 14
- 102000004196 processed proteins & peptides Human genes 0.000 claims 14
- 108090000765 processed proteins & peptides Proteins 0.000 claims 14
- 238000000034 method Methods 0.000 claims 7
- 238000002360 preparation method Methods 0.000 claims 5
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 4
- 238000000855 fermentation Methods 0.000 claims 4
- 230000004151 fermentation Effects 0.000 claims 4
- 238000004519 manufacturing process Methods 0.000 claims 3
- 102000040430 polynucleotide Human genes 0.000 claims 3
- 108091033319 polynucleotide Proteins 0.000 claims 3
- 239000002157 polynucleotide Substances 0.000 claims 3
- 239000000758 substrate Substances 0.000 claims 3
- 108010059892 Cellulase Proteins 0.000 claims 2
- 108010008885 Cellulose 1,4-beta-Cellobiosidase Proteins 0.000 claims 2
- 108010055059 beta-Mannosidase Proteins 0.000 claims 2
- 229940106157 cellulase Drugs 0.000 claims 2
- 108010002430 hemicellulase Proteins 0.000 claims 2
- 229940059442 hemicellulase Drugs 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 150000007523 nucleic acids Chemical class 0.000 claims 2
- 238000006467 substitution reaction Methods 0.000 claims 2
- 101001065065 Aspergillus awamori Feruloyl esterase A Proteins 0.000 claims 1
- 102100032487 Beta-mannosidase Human genes 0.000 claims 1
- 108010001817 Endo-1,4-beta Xylanases Proteins 0.000 claims 1
- 101710111935 Endo-beta-1,4-glucanase Proteins 0.000 claims 1
- 108090000371 Esterases Proteins 0.000 claims 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 1
- 229920002488 Hemicellulose Polymers 0.000 claims 1
- 101710154526 Lytic chitin monooxygenase Proteins 0.000 claims 1
- 108010059820 Polygalacturonase Proteins 0.000 claims 1
- 108010093941 acetylxylan esterase Proteins 0.000 claims 1
- 102000005840 alpha-Galactosidase Human genes 0.000 claims 1
- 108010030291 alpha-Galactosidase Proteins 0.000 claims 1
- 108010084650 alpha-N-arabinofuranosidase Proteins 0.000 claims 1
- 102000005936 beta-Galactosidase Human genes 0.000 claims 1
- 108010005774 beta-Galactosidase Proteins 0.000 claims 1
- 229920002678 cellulose Polymers 0.000 claims 1
- 239000001913 cellulose Substances 0.000 claims 1
- 108010080434 cephalosporin-C deacetylase Proteins 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 108010038658 exo-1,4-beta-D-xylosidase Proteins 0.000 claims 1
- 108010093305 exopolygalacturonase Proteins 0.000 claims 1
- 230000002538 fungal effect Effects 0.000 claims 1
- 239000008103 glucose Substances 0.000 claims 1
- 230000005764 inhibitory process Effects 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2445—Beta-glucosidase (3.2.1.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Claims (12)
1. Varijantni polipeptid koji sadrži supstituciju G142S na položaju koji odgovara položaju 142 polipeptida iz SEQ ID NO: 2, pri čemu varijantni polipeptid ima aktivnost beta-glukozidaze i najmanje 80% identičnosti sekvence s polipeptidom iz SEQ ID NO: 2 i pri čemu je varijanta polipeptida manje osjetljiva na inhibiciju glukoze u odnosu na matični polipeptid prikazan u SEQ ID NO: 2.
2. Varijantni polipeptid prema patentnom zahtjevu 1, naznačena time što nadalje sadrži supstituciju na položaju koji odgovara položaju 90, 103, 335, 485 i/ili 606 polipeptida iz SEQ ID NO: 2.
3. Polinukleotid naznačen time što kodira varijantni polipeptid prema patentnom zahtjevu 1 ili 2.
4. Konstrukt nukleinske kiseline ili vektor naznačen time što sadrži polinukleotid prema patentnom zahtjevu 3.
5. Stanica domaćina naznačena time što sadrži polinukleotid prema patentnom zahtjevu 3 ili konstrukt nukleinske kiseline ili vektor prema patentnom zahtjevu 4.
6. Stanica domaćina prema patentnom zahtjevu 5, naznačena time što je stanica gljivična stanica.
7. Postupak za proizvodnju varijantnog polipeptida prema patentnom zahtjevu 1 ili 2, naznačen time što postupak obuhvaća korake:
a) kultiviranje stanice domaćina prema patentnom zahtjevu 5 ili 6 pod uvjetima koji pogoduju proizvodnji varijantnog polipeptida, i
b) po izboru, oporavljanje varijantnog polipeptida.
8. Pripravak naznačen time što sadrži:
(i) varijantni polipeptid prema patentnom zahtjevu 1 ili 2, i
(ii) celulazu i/ili hemicelulazu i/ili pektinazu.
9. Pripravak prema patentnom zahtjevu 8, naznačen time što je celulaza odabrana iz skupine koja sadrži litički polisaharid monooksigenazu, celobiohidrolazu I, celobiohidrolazu II, endo-beta-1,4-glukanazu, beta-glukozidazu i beta-(1,3)(1,4)-glukanazu ili bilo koju njihovu kombinaciju i hemicelulaza je odabrana iz skupine koja sadrži endoksilanazu, beta-ksilozidazu, alfa-L-arabinofuranozidazu, alfa-D-glukuronidazu, acetil-ksilan esterazu, feruloil esterazu, kumaroil esterazu, alfa-galaktozidazu, beta-galaktozidazu, beta-mananazu, beta-manozidazu ili bilo koju njihovu kombinaciju.
10. Pripravak prema patentnom zahtjevu 8 ili 9, naznačen time što da je pripravak čitava fermentacijska juha.
11. Postupak za obradu supstrata koji sadrži celulozu i/ili hemicelulozu koji postupak uključuje korak dovođenja u kontakt supstrata s varijantnim polipeptidom prema patentnom zahtjevu 1 ili 2 i/ili s pripravkom prema bilo kojem od patentnih zahtjeva 8 do 10.
12. Postupak proizvodnje fermentacijskog proizvoda, koji postupak uključuje slijedeće korake:
a) tretiranje supstrata korištenjem postupka u skladu s patentnim zahtjevom 11, i
b) fermentiranje dobivenog materijala radi dobivanja fermentacijskog proizvoda.
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP15198788 | 2015-12-10 | ||
| EP15198826 | 2015-12-10 | ||
| EP15198893 | 2015-12-10 | ||
| EP15198902 | 2015-12-10 | ||
| EP16816610.6A EP3387139B1 (en) | 2015-12-10 | 2016-12-08 | Beta-glucosidase and uses thereof |
| PCT/EP2016/080240 WO2017097895A1 (en) | 2015-12-10 | 2016-12-08 | Beta-glucosidase and uses thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20200975T1 true HRP20200975T1 (hr) | 2020-10-16 |
Family
ID=57609853
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20200975TT HRP20200975T1 (hr) | 2015-12-10 | 2020-06-19 | Beta-glukozidaza i njezina upotreba |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US10808235B2 (hr) |
| EP (2) | EP3702469A1 (hr) |
| CN (1) | CN108368530A (hr) |
| BR (1) | BR112018011097A2 (hr) |
| DK (1) | DK3387139T3 (hr) |
| HR (1) | HRP20200975T1 (hr) |
| PL (1) | PL3387139T3 (hr) |
| WO (1) | WO2017097895A1 (hr) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109770209B (zh) * | 2019-02-23 | 2022-09-30 | 北京武亿丰食品有限公司 | 一种风味馒头加工工艺 |
| CN110540980B (zh) * | 2019-09-07 | 2021-06-11 | 华南理工大学 | 一种海洋链霉菌脂肪酶突变体及其应用 |
| CN114369558B (zh) * | 2022-03-22 | 2022-06-07 | 华南理工大学 | 一株粘质沙雷氏菌及其在生产柚苷酶中的应用 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7413888B2 (en) * | 2003-05-02 | 2008-08-19 | Novozymes, Inc. | Variants of beta-glucosidases |
| DK2599862T3 (da) * | 2009-11-20 | 2017-11-06 | Danisco Us Inc | Beta-glucosidasevarianter med forbedrede egenskaber |
| MX2012015142A (es) | 2010-06-29 | 2013-05-01 | Dsm Ip Assets Bv | Polipeptidos que tiene actividad beta-glucosidasa y usos del mismo. |
| BR112014004186B1 (pt) * | 2011-08-24 | 2020-12-15 | Novozymes, Inc. | Composição enzimática, célula hospedeira de fungo filamentoso recombinante, método para produzir uma composição enzimática, processos para degradar um material celulósico, para sintetizar um produto de fermentação, e, para fermentar um material celulósico |
-
2016
- 2016-12-08 US US15/781,857 patent/US10808235B2/en active Active
- 2016-12-08 CN CN201680071634.3A patent/CN108368530A/zh not_active Withdrawn
- 2016-12-08 EP EP20163501.8A patent/EP3702469A1/en not_active Withdrawn
- 2016-12-08 WO PCT/EP2016/080240 patent/WO2017097895A1/en active Application Filing
- 2016-12-08 DK DK16816610.6T patent/DK3387139T3/da active
- 2016-12-08 BR BR112018011097A patent/BR112018011097A2/pt not_active Application Discontinuation
- 2016-12-08 PL PL16816610T patent/PL3387139T3/pl unknown
- 2016-12-08 EP EP16816610.6A patent/EP3387139B1/en active Active
-
2020
- 2020-06-19 HR HRP20200975TT patent/HRP20200975T1/hr unknown
- 2020-08-31 US US17/008,565 patent/US20200392475A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US10808235B2 (en) | 2020-10-20 |
| PL3387139T3 (pl) | 2020-11-02 |
| EP3387139B1 (en) | 2020-04-22 |
| WO2017097895A1 (en) | 2017-06-15 |
| US20180362945A1 (en) | 2018-12-20 |
| CN108368530A (zh) | 2018-08-03 |
| EP3702469A1 (en) | 2020-09-02 |
| EP3387139A1 (en) | 2018-10-17 |
| BR112018011097A2 (pt) | 2018-12-04 |
| US20200392475A1 (en) | 2020-12-17 |
| DK3387139T3 (da) | 2020-07-20 |
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