HRP20180031T1 - Method of enhancing cell growth using alkyl-amine-n-oxide (aanox) - Google Patents

Method of enhancing cell growth using alkyl-amine-n-oxide (aanox) Download PDF

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Publication number
HRP20180031T1
HRP20180031T1 HRP20180031TT HRP20180031T HRP20180031T1 HR P20180031 T1 HRP20180031 T1 HR P20180031T1 HR P20180031T T HRP20180031T T HR P20180031TT HR P20180031 T HRP20180031 T HR P20180031T HR P20180031 T1 HRP20180031 T1 HR P20180031T1
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Croatia
Prior art keywords
cells
oxide
factor
ddao
aanox
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HRP20180031TT
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Croatian (hr)
Inventor
Sylvain Roy
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Baxalta GmbH
Baxalta Incorporated
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Application filed by Baxalta GmbH, Baxalta Incorporated filed Critical Baxalta GmbH
Publication of HRP20180031T1 publication Critical patent/HRP20180031T1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/005—Protein-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0043—Medium free of human- or animal-derived components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0031—Serum-free culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/76—Undefined extracts from plants

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Claims (13)

1. Postupak poboljšavanja eksprimiranja rekombinantnog čimbenika zgrušavanja krvi u kulturi stanica koja sadrži uzgajane stanice koje eksprimiraju rekombinantni čimbenik zgrušavanja krvi u mediju za uzgoj u kulturi koja sadrži količinu alkil-amin-N-oksida (AANOx) dovoljna za poboljšavanje stope rasta stanica u kulturi, naznačen time što se količina AANOx kreće između 4 i 80 ppb, te što je AANOx DDAO ili srodni analit koji se bira iz skupine koju čine dimetil(tetradecil)amin-oksid (C14NC2O), dimetil(heksadecil)amin-oksid (C16NC2O), a analite AANOx se bira iz skupine koju čine C10 dimetilamin-oksid, C12 dimetilamin-oksid, C14 dimetilamin-oksid i C16 dimetilamin-oksid.1. A method of improving the expression of a recombinant blood coagulation factor in a cell culture containing cultured cells expressing a recombinant blood coagulation factor in a culture medium containing an amount of alkyl-amine-N-oxide (AANOx) sufficient to improve the growth rate of the cells in the culture, indicated by the fact that the amount of AANOx ranges between 4 and 80 ppb, and which is AANOx DDAO or a related analyte selected from the group consisting of dimethyl(tetradecyl)amine oxide (C14NC2O), dimethyl(hexadecyl)amine oxide (C16NC2O), and the analyte AANOx selected from the group consisting of C10 dimethylamine oxide, C12 dimethylamine oxide, C14 dimethylamine oxide and C16 dimethylamine oxide. 2. Postupak poboljšavanja stope rasta stanica u kulturi stanica koja sadrži uzgajane stanice u mediju za uzgoj u kulturi koja sadrži količinu alkil-amin-N-oksida (AANOx) dovoljna za poboljšavanje stope rasta stanica u kulturi, naznačen time što se količina AANOx kreće između 4 i 80 ppb, te što je AANOx DDAO ili srodni analit koji se bira iz skupine koju čine dimetil(tetradecil)amin-oksid (C14NC2O), dimetil(heksadecil)amin-oksid (C16NC2O), a analite AANOx se bira iz skupine koju čine C10 dimetilamin-oksid, C12 dimetilamin-oksid, C14 dimetilamin-oksid i C16 dimetilamin-oksid.2. A method of improving the growth rate of cells in a cell culture containing cultured cells in a culture medium containing an amount of alkyl-amine-N-oxide (AANOx) sufficient to improve the growth rate of cells in culture, indicated by the fact that the amount of AANOx ranges between 4 and 80 ppb, and which is AANOx DDAO or a related analyte selected from the group consisting of dimethyl(tetradecyl)amine oxide (C14NC2O), dimethyl(hexadecyl)amine oxide (C16NC2O), and the analyte AANOx selected from the group consisting of C10 dimethylamine oxide, C12 dimethylamine oxide, C14 dimethylamine oxide and C16 dimethylamine oxide. 3. Postupak u skladu s patentnim zahtjevom 1 ili 2, naznačen time što se količina dodecildimetilamin-oksida (DDAO) ili srodnog analita kreće između 4 i 50 ppb, primjerice između 10 ppb i 40 ppb.3. The method according to claim 1 or 2, characterized in that the amount of dodecyldimethylamine oxide (DDAO) or a related analyte ranges between 4 and 50 ppb, for example between 10 ppb and 40 ppb. 4. Postupak u skladu s patentnim zahtjevom 1 ili 2, naznačen time što se dodecildimetilamin-oksid (DDAO) ili srodni analit dodaje kroz pravilne vremenske periode radi održavanja razine DDAO ili srodni analit u mediju za stanice.4. The method according to claim 1 or 2, characterized in that dodecyldimethylamine oxide (DDAO) or a related analyte is added at regular intervals to maintain the level of DDAO or a related analyte in the cell medium. 5. Postupak u skladu s patentnim zahtjevom 1 ili 2, naznačen time što se dodecildimetilamin-oksid (DDAO) ili srodni analit dodaje kroz više vremenskih perioda radi održavanja razine DDAO ili srodni analit u mediju za stanice.5. The method according to claim 1 or 2, characterized in that dodecyldimethylamine oxide (DDAO) or a related analyte is added over several time periods to maintain the level of DDAO or a related analyte in the cell medium. 6. Postupak u skladu s patentnim zahtjevom 1 ili 2, naznačen time što se dodecildimetilamin-oksid (DDAO) ili srodni analit dodaje kontinuirano kroz jedan vremenski period radi održavanja razine DDAO ili srodni analit u mediju za stanice.6. The method according to claim 1 or 2, characterized in that dodecyldimethylamine oxide (DDAO) or a related analyte is added continuously over a period of time to maintain the level of DDAO or a related analyte in the cell medium. 7. Postupak u skladu s patentnim zahtjevom 1 ili 2, naznačen time što se dodecildimetilamin-oksid (DDAO) ili srodni analit ne dobiva iz hidrolizatnog pripravka soje.7. The method according to claim 1 or 2, characterized in that dodecyldimethylamine oxide (DDAO) or a related analyte is not obtained from the soy hydrolyzate preparation. 8. Postupak u skladu s patentnim zahtjevom 1 ili 2, naznačen time što se dodecildimetilamin-oksid (DDAO) ili srodni analit dobiva iz hidrolizatnog pripravka soje.8. The method according to claim 1 or 2, characterized in that dodecyldimethylamine oxide (DDAO) or a related analyte is obtained from a soy hydrolyzate preparation. 9. Postupak u skladu s patentnim zahtjevom 1 ili 2, naznačen time što je medij za uzgoj u kulturi medij bez životinjskog proteina.9. The method according to claim 1 or 2, characterized in that the culture medium is a medium without animal protein. 10. Postupak u skladu s patentnim zahtjevom 1 ili 2, naznačen time što medij za uzgoj u kulturi sadrži životinjski protein.10. The method according to claim 1 or 2, characterized in that the culture medium contains animal protein. 11. Postupak u skladu s patentnim zahtjevom 1 ili 2, naznačen time što su stanice sisavačke stanice, primjerice stanice BSC, stanice LLC-MK, stanice CV-1, stanice COS, stanice VERO, stanice MDBK, stanice MDCK, stanice CRFK, stanice RAF, stanice RK, stanice TCMK-1, stanice LLCPK, stanice PK15, stanice LLC-RK, stanice MDOK, stanice BHK-21, stanice CHO, stanice NS-1, stanice MRC-5, stanice WI-38, stanice BHK, stanice 293 ili stanice RK.11. The method according to claim 1 or 2, characterized in that the cells are mammalian cells, for example BSC cells, LLC-MK cells, CV-1 cells, COS cells, VERO cells, MDBK cells, MDCK cells, CRFK cells, RAF, RK cells, TCMK-1 cells, LLCPK cells, PK15 cells, LLC-RK cells, MDOK cells, BHK-21 cells, CHO cells, NS-1 cells, MRC-5 cells, WI-38 cells, BHK cells, station 293 or station RK. 12. Postupak u skladu s patentnim zahtjevom 1 ili 2, naznačen time što se rekombinantni čimbenik zgrušavanja krvi bira iz skupine koju čine Čimbenik II, Čimbenik V, Čimbenik VII, Čimbenik VIII, Čimbenik IX, Čimbenik X, Čimbenik XI, von Willebrandov čimbenik, Čimbenik XII i Čimbenik XIII.12. The method according to patent claim 1 or 2, characterized in that the recombinant blood clotting factor is selected from the group consisting of Factor II, Factor V, Factor VII, Factor VIII, Factor IX, Factor X, Factor XI, von Willebrand factor, Factor XII and Factor XIII. 13. Postupak u skladu s patentnim zahtjevom 1, naznačen time što je AANOx dimetil(tetradecil)amin-oksid ili dimetil(heksadecil)amin-oksid.13. The method according to claim 1, characterized in that AANOx is dimethyl(tetradecyl)amine oxide or dimethyl(hexadecyl)amine oxide.
HRP20180031TT 2008-12-30 2018-01-09 Method of enhancing cell growth using alkyl-amine-n-oxide (aanox) HRP20180031T1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14155508P 2008-12-30 2008-12-30
EP09798998.2A EP2373783B1 (en) 2008-12-30 2009-12-30 Method of enhancing cell growth using alkyl-amine-n-oxide (aanox)
PCT/US2009/069854 WO2010078450A1 (en) 2008-12-30 2009-12-30 Method of enhancing cell growth using alkyl-amine-n-oxide (aanox)

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HRP20180031T1 true HRP20180031T1 (en) 2018-04-20

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US (2) US9051544B2 (en)
EP (1) EP2373783B1 (en)
JP (1) JP5764068B2 (en)
KR (1) KR101737645B1 (en)
AU (1) AU2009334512B2 (en)
CA (1) CA2751021A1 (en)
DK (1) DK2373783T3 (en)
HR (1) HRP20180031T1 (en)
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WO (1) WO2010078450A1 (en)

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US10047134B2 (en) * 2013-01-10 2018-08-14 Biocon Limited Process for expression of recombinant proteins in Pichia pastoris using a fed batch model

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HUE036415T2 (en) 2018-07-30
US9051544B2 (en) 2015-06-09
KR20110110271A (en) 2011-10-06
KR101737645B1 (en) 2017-05-18
DK2373783T3 (en) 2018-01-29
AU2009334512B2 (en) 2014-04-24
EP2373783B1 (en) 2017-11-01
WO2010078450A1 (en) 2010-07-08
CA2751021A1 (en) 2010-07-08
US20150252321A1 (en) 2015-09-10
EP2373783A1 (en) 2011-10-12
US9464269B2 (en) 2016-10-11
JP5764068B2 (en) 2015-08-12
US20100233805A1 (en) 2010-09-16
AU2009334512A1 (en) 2011-07-21
JP2012513770A (en) 2012-06-21

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