HRP20170096T1 - Fermentativna proizvodnja izobutanola uz pomoć kvasca - Google Patents
Fermentativna proizvodnja izobutanola uz pomoć kvasca Download PDFInfo
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- HRP20170096T1 HRP20170096T1 HRP20170096TT HRP20170096T HRP20170096T1 HR P20170096 T1 HRP20170096 T1 HR P20170096T1 HR P20170096T T HRP20170096T T HR P20170096TT HR P20170096 T HRP20170096 T HR P20170096T HR P20170096 T1 HRP20170096 T1 HR P20170096T1
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- ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 title claims 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims 2
- 238000012262 fermentative production Methods 0.000 title 1
- 210000005253 yeast cell Anatomy 0.000 claims 15
- AMIMRNSIRUDHCM-UHFFFAOYSA-N Isopropylaldehyde Chemical compound CC(C)C=O AMIMRNSIRUDHCM-UHFFFAOYSA-N 0.000 claims 6
- 210000004027 cell Anatomy 0.000 claims 6
- 108090000623 proteins and genes Proteins 0.000 claims 6
- 238000006243 chemical reaction Methods 0.000 claims 5
- 108090000790 Enzymes Proteins 0.000 claims 4
- 102000004190 Enzymes Human genes 0.000 claims 4
- 108010004277 2-acetolactate mutase Proteins 0.000 claims 3
- WTLNOANVTIKPEE-UHFFFAOYSA-N 2-acetyloxypropanoic acid Chemical compound OC(=O)C(C)OC(C)=O WTLNOANVTIKPEE-UHFFFAOYSA-N 0.000 claims 3
- QHKABHOOEWYVLI-UHFFFAOYSA-N 3-methyl-2-oxobutanoic acid Chemical compound CC(C)C(=O)C(O)=O QHKABHOOEWYVLI-UHFFFAOYSA-N 0.000 claims 3
- 230000000694 effects Effects 0.000 claims 3
- 101150026777 ADH5 gene Proteins 0.000 claims 2
- 108010000700 Acetolactate synthase Proteins 0.000 claims 2
- 102000007698 Alcohol dehydrogenase Human genes 0.000 claims 2
- 108010021809 Alcohol dehydrogenase Proteins 0.000 claims 2
- 108700016168 Dihydroxy-acid dehydratases Proteins 0.000 claims 2
- 101100396751 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) ilv-2 gene Proteins 0.000 claims 2
- 241000235648 Pichia Species 0.000 claims 2
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 claims 2
- XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 claims 2
- 230000002438 mitochondrial effect Effects 0.000 claims 2
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 claims 2
- 230000008685 targeting Effects 0.000 claims 2
- JTEYKUFKXGDTEU-UHFFFAOYSA-N 2,3-dihydroxy-3-methylbutanoic acid Chemical compound CC(C)(O)C(O)C(O)=O JTEYKUFKXGDTEU-UHFFFAOYSA-N 0.000 claims 1
- 101150078509 ADH2 gene Proteins 0.000 claims 1
- 101150089022 ADH4 gene Proteins 0.000 claims 1
- 101150006123 ADH7 gene Proteins 0.000 claims 1
- 108700028369 Alleles Proteins 0.000 claims 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 1
- 101710088194 Dehydrogenase Proteins 0.000 claims 1
- 101100269269 Drosophila mayaguana Adh gene Proteins 0.000 claims 1
- 108010035824 Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) Proteins 0.000 claims 1
- 102000003679 Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) Human genes 0.000 claims 1
- 108090000094 Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) Proteins 0.000 claims 1
- 101150111679 ILV5 gene Proteins 0.000 claims 1
- 101100054943 Mus musculus Adh4 gene Proteins 0.000 claims 1
- 101100001026 Mus musculus Adh7 gene Proteins 0.000 claims 1
- BAWFJGJZGIEFAR-NNYOXOHSSA-O NAD(+) Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-O 0.000 claims 1
- 101150050255 PDC1 gene Proteins 0.000 claims 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims 1
- 101100396749 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ILV3 gene Proteins 0.000 claims 1
- 101100082596 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PDC5 gene Proteins 0.000 claims 1
- 101100519200 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PDC6 gene Proteins 0.000 claims 1
- 241000235013 Yarrowia Species 0.000 claims 1
- 229910052799 carbon Inorganic materials 0.000 claims 1
- 101150045896 ilv-2 gene Proteins 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 230000002503 metabolic effect Effects 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 claims 1
- 150000007523 nucleic acids Chemical group 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 238000006491 synthase reaction Methods 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/16—Butanols
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Claims (11)
1. Stanica kvasca koja proizvodi izobutanol. naznačena time što stanica ima povećan metabolički tijek materijala od piruvata preko acetolaktata. 2.3-dihidroksiizovalerata. 2-ketoizovalerata, izobutiraldehida do izobutanola. gdje se sve gene koji kodiraju enzime koji sudjeluju u toj pretvorbi hipereksprimira, te gdje su svi ti geni homologni s navedenom stanicom kvasca, gdje Ilv2 s SEQ ID No. 2 katalizira acetolaktat-sintaznu reakciju iz piruvata u acetolaktat, Ilv5 s SEQ ID No. 6 katalizira acetohidroksikiselina-reduktoizomeraznu reakciju iz acetolaktata u 2,3-dihidroksiizovalerat. Ilv3 s SEO ID No. 8 katalizira dihidroksikiselina-dehidrataznu reakciju iz 2.3-dihidroksiizovalerata u 2-ketoizovalerat. 2-ketokiselina-dekarboksilaza katalizira reakciju iz 2-ketoizovalerata u izobutiraldehid. a alkohol-dehidrogenaza katalizira reakciju iz izobutiraldehida u izobutanol, gdje je bilo najmanje jedan od promotora tih gena zamijenjen najmanje jednim jačim promotorom, ili su nukleinskokiselinski sljedovi tih gena pretvoreni u kodonski optimizirane alele, što se geni ILV2, ILV5 i ILV3 hipereksprimiraju bez mitohondijski ciljajućeg slijeda kod enzima acetolaktat-sintetaze. acetohidroksikiselina-reduktoizomeraze i dihidroksikiselina-dehidrataze. ili s uništenim. inaktiviranim mitohondijski ciljajućim slijedom, i što je uz eksprimiranje gena PDC1 s SEQ ID No. 13. PDC5 s SEO ID No. 15 i PDC6 s SEQ ID No. 17 smanjena odnosno isključena aktivnost kodiranih enzima.
2. Stanica kvasca u skladu s patentnim zahtjevom 1, naznačena time što se 2-ketokiselina-dekarboksilaza bira između najmanje jednog od enzima Aro 10 s SEQ ID No. 20 ili Thi3 s SEQ ID No. 22.
3. Stanica kvasca u skladu s bilo kojim od patentnih zahtjeva 1 ili 2, naznačena time što se alkohol-dehidrogenaza bira između najmanje jednog od enzima Adhl s SEQ ID No. 24. Adh2 s SEQ ID No. 26, Adh3 s SEQ ID No. 28. Adh4 s SEO ID No. 30. Adh5 s SEO ID No. 32. Adh6 s SEQ ID No. 34. Adh7 s SEO ID No. 36. Sfal s SEQ ID No. 38 ili Yprl s SEQ ID No. 40.
4. Stanica kvasca u skladu s bilo kojim od patentnih zahtjeva 1 do 3, naznačena time što se sve hipereksprimirane gene hipereksprimira u kodonski optimiziranim varijantama.
5. Stanica kvasca u skladu s bilo kojim od patentnih zahtjeva 1 do 4. naznačena time što stanica eksprimira acetohidroksikiseiina-reduktoizomerazu, s povećanom specifičnošću za NADH u usporedbi s NADPH.
6. Stanica kvasca u skladu s bilo kojim od patentnih zahtjeva 1 do 4. naznačena time što stanica također eksprimira fosforilativnu gliceraldehid-3-fosfat-dehidrogenazu. s povećanom specifičnošću za NADP+ u usporedbi s NAD+.
7. Stanica kvasca u skladu s patentnim zahtjevom 6. naznačena time što se tu NADP-gliceraldehid-3-fosfat-dehidrogenazu eksprimira u stanici kvasca kod koje nema ekspresije ili aktivnosti, ili je smanjena ekspresija ili aktivnost NAD-gliceraldehid-3-tbsfat-dehidrogenaza.
8. Stanica kvasca u skladu s bilo kojim od patentnih zahtjeva 1 do 7, naznačena time što se dodatno hipereksprimira protein Ilv6 s SEQ ID No. 4. u istom staničnom odjeljku kao Ilv2.
9. Stanica kvasca u skladu s bilo kojim od patentnih zahtjeva 1 do 8. naznačena time što se stanica bira iz sljedeće skupine: Pichia, Candida, Hansenula, Kluyveromices, Yarrowia i Saccharomices.
10. Stanica kvasca u skladu s patentnim zahtjevom 9. naznačena time stoje stanica domaćin Saccharomices cerevisiae.
11. Postupak proizvodnje izobutanola uz pomoć stanica kvasca, naznačen time što se sastoji u osiguravanju stanice kvasca u skladu s bilo kojim od patentnih zahtjeva 1 do 10. kao i u dovođenju stanice kvasca u kontakt s fermentabilnim izvorom ugljika.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102008010121A DE102008010121B4 (de) | 2008-02-20 | 2008-02-20 | Fermentative Produktion von Isobutanol mit Hefe |
| EP09713531.3A EP2255007B1 (de) | 2008-02-20 | 2009-02-19 | Fermentative produktion von isobutanol mit hefe |
| PCT/EP2009/001191 WO2009103533A1 (de) | 2008-02-20 | 2009-02-19 | Fermentative produktion von isobutanol mit hefe |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20170096T1 true HRP20170096T1 (hr) | 2017-03-24 |
Family
ID=40718833
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20170096TT HRP20170096T1 (hr) | 2008-02-20 | 2017-01-20 | Fermentativna proizvodnja izobutanola uz pomoć kvasca |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US8530226B2 (hr) |
| EP (1) | EP2255007B1 (hr) |
| BR (1) | BRPI0913670B1 (hr) |
| CA (1) | CA2716427C (hr) |
| DE (1) | DE102008010121B4 (hr) |
| DK (1) | DK2255007T3 (hr) |
| ES (1) | ES2613539T3 (hr) |
| HR (1) | HRP20170096T1 (hr) |
| HU (1) | HUE031212T2 (hr) |
| PL (1) | PL2255007T3 (hr) |
| PT (1) | PT2255007T (hr) |
| WO (1) | WO2009103533A1 (hr) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9303225B2 (en) | 2005-10-26 | 2016-04-05 | Butamax Advanced Biofuels Llc | Method for the production of isobutanol by recombinant yeast |
| UA96928C2 (ru) | 2005-10-26 | 2011-12-26 | Э.И. Дю Пон Де Немур Энд Компани | Ферментативное продуцирование спиртов с четырьмя атомами углерода |
| US8273558B2 (en) | 2005-10-26 | 2012-09-25 | Butamax(Tm) Advanced Biofuels Llc | Fermentive production of four carbon alcohols |
| EP2235193B1 (en) | 2007-12-23 | 2015-11-25 | GEVO, Inc. | Yeast organism producing isobutanol at a high yield |
| US8455239B2 (en) | 2007-12-23 | 2013-06-04 | Gevo, Inc. | Yeast organism producing isobutanol at a high yield |
| EP2238098A4 (en) | 2007-12-27 | 2016-06-01 | Gevo Inc | RECOVERING HIGHER ALCOHOLS IN DILUTED AQUEOUS SOLUTIONS |
| BRPI0909989A2 (pt) * | 2008-06-05 | 2021-06-22 | Butamax Advanced Biofuels Llc | célula de levedura recombinante e método para a produção de um produto |
| US20160222370A1 (en) * | 2008-09-29 | 2016-08-04 | Butamax Advanced Biofuels Llc | Recombinant Yeast Host Cell With Fe-S Cluster Proteins And Methods Of Using Thereof |
| ES2521675T3 (es) | 2008-09-29 | 2014-11-13 | Butamax Advanced Biofuels Llc | Identificación y uso de [2Fe-2S]-dihidroxi-ácido deshidratasas bacterianas |
| AU2009296224B2 (en) | 2008-09-29 | 2015-02-12 | Butamax(Tm) Advanced Biofuels Llc | Increased heterologous Fe-S enzyme actiivty in yeast |
| BR112012003241A2 (pt) | 2009-08-12 | 2015-09-01 | Gevo Inc | Localização de via de isobutanol citosólico para a produção de isobutanol |
| EP2504422B1 (en) | 2009-11-24 | 2016-01-27 | GEVO, Inc. | Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids |
| US20120034666A1 (en) * | 2009-11-24 | 2012-02-09 | Gevo, Inc. | Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids |
| WO2011073250A2 (en) | 2009-12-15 | 2011-06-23 | Stratley Ag | Method for recovery of organic components from dilute aqueous solutions |
| US20110201090A1 (en) * | 2010-02-12 | 2011-08-18 | Gevo, Inc. | Yeast microorganisms with reduced by-product accumulation for improved production of fuels, chemicals, and amino acids |
| JP5950830B2 (ja) * | 2010-02-17 | 2016-07-13 | ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー | Ｆｅ−Ｓクラスター要求タンパク質の活性の改善 |
| EP2575499A2 (en) | 2010-05-31 | 2013-04-10 | Vib Vzw | The use of transporters to modulate flavor production by yeast |
| US9012190B2 (en) | 2011-06-15 | 2015-04-21 | Butamax Advanced Biofuels Llc | Use of thiamine and nicotine adenine dinucleotide for butanol production |
| KR20130105649A (ko) * | 2010-09-07 | 2013-09-25 | 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 | 피루베이트로부터 아세토락테이트로의 전환을 촉매작용시키는 폴리펩티드를 암호화하는 폴리뉴클레오티드의 통합 |
| EP2508597A1 (en) | 2011-04-05 | 2012-10-10 | Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) | Production of butanol by fermentation in Arxula sp. |
| BR112013032320A2 (pt) * | 2011-06-17 | 2016-12-20 | Butamax Advanced Biofuels Llc | método e composição de produção de butanol, composição de butanol e isobutanol, método de cultivo de um micro-organismo produtor de butanol em material lignocelulósico e método para inibir o crescimento de micro-organismos que não produzem butanol em uma composição |
| US20140329275A1 (en) * | 2011-10-10 | 2014-11-06 | Regents Of The University Of Minnesota | Biocatalysis cells and methods |
| EP2583957A1 (de) | 2011-10-18 | 2013-04-24 | LANXESS Deutschland GmbH | Lineare Butene aus Isobutanol |
| WO2013102147A2 (en) | 2011-12-30 | 2013-07-04 | Butamax (Tm) Advanced Biofuels Llc | Genetic switches for butanol production |
| US9650624B2 (en) | 2012-12-28 | 2017-05-16 | Butamax Advanced Biofuels Llc | DHAD variants for butanol production |
| WO2014151190A1 (en) | 2013-03-15 | 2014-09-25 | Butamax Advanced Biofuels Llc | Dhad variants and methods of screening |
| WO2014144210A2 (en) * | 2013-03-15 | 2014-09-18 | Butamax Advanced Biofuels Llc | Competitive growth and/or production advantage for butanologen microorganism |
| WO2015002913A1 (en) | 2013-07-03 | 2015-01-08 | Butamax Advanced Biofuels Llc | Partial adaptation for butanol production |
| WO2018112636A1 (en) | 2016-12-21 | 2018-06-28 | Creatus Biosciences Inc. | Method and organism expressing metschnikowia xylose transporters for increased xylose uptake |
| CA3187165A1 (en) | 2020-06-15 | 2021-12-23 | Melio Peptide Systems Inc. | Microorganisms and methods for reducing bacterial contamination |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| UA96928C2 (ru) * | 2005-10-26 | 2011-12-26 | Э.И. Дю Пон Де Немур Энд Компани | Ферментативное продуцирование спиртов с четырьмя атомами углерода |
-
2008
- 2008-02-20 DE DE102008010121A patent/DE102008010121B4/de active Active
-
2009
- 2009-02-19 BR BRPI0913670-3A patent/BRPI0913670B1/pt active IP Right Grant
- 2009-02-19 PL PL09713531T patent/PL2255007T3/pl unknown
- 2009-02-19 HU HUE09713531A patent/HUE031212T2/en unknown
- 2009-02-19 US US12/918,722 patent/US8530226B2/en active Active
- 2009-02-19 PT PT97135313T patent/PT2255007T/pt unknown
- 2009-02-19 DK DK09713531.3T patent/DK2255007T3/en active
- 2009-02-19 ES ES09713531.3T patent/ES2613539T3/es active Active
- 2009-02-19 EP EP09713531.3A patent/EP2255007B1/de active Active
- 2009-02-19 WO PCT/EP2009/001191 patent/WO2009103533A1/de active Application Filing
- 2009-02-19 CA CA2716427A patent/CA2716427C/en active Active
-
2017
- 2017-01-20 HR HRP20170096TT patent/HRP20170096T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CA2716427A1 (en) | 2009-08-27 |
| HUE031212T2 (en) | 2017-07-28 |
| DE102008010121A1 (de) | 2009-09-03 |
| DK2255007T3 (en) | 2017-02-06 |
| WO2009103533A1 (de) | 2009-08-27 |
| EP2255007B1 (de) | 2016-11-02 |
| CA2716427C (en) | 2017-08-29 |
| PL2255007T3 (pl) | 2017-06-30 |
| BRPI0913670A2 (pt) | 2016-07-26 |
| ES2613539T3 (es) | 2017-05-24 |
| EP2255007A1 (de) | 2010-12-01 |
| DE102008010121B4 (de) | 2013-11-21 |
| US20110053235A1 (en) | 2011-03-03 |
| BRPI0913670B1 (pt) | 2020-10-20 |
| PT2255007T (pt) | 2017-02-08 |
| US8530226B2 (en) | 2013-09-10 |
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