HK1258715A1 - 用於生產核酸和蛋白質的裝置和方法 - Google Patents

用於生產核酸和蛋白質的裝置和方法

Info

Publication number
HK1258715A1
HK1258715A1 HK19101116.6A HK19101116A HK1258715A1 HK 1258715 A1 HK1258715 A1 HK 1258715A1 HK 19101116 A HK19101116 A HK 19101116A HK 1258715 A1 HK1258715 A1 HK 1258715A1
Authority
HK
Hong Kong
Prior art keywords
proteins
methods
devices
nucleic acids
producing nucleic
Prior art date
Application number
HK19101116.6A
Other languages
English (en)
Inventor
Claudia Chiocchini
Axel Trefzer
Krishna Vattem
Phillip Kuhn
Original Assignee
Pierce Biotechnology Inc
Thermo Fisher Scient Geneart Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pierce Biotechnology Inc, Thermo Fisher Scient Geneart Gmbh filed Critical Pierce Biotechnology Inc
Publication of HK1258715A1 publication Critical patent/HK1258715A1/zh

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/005—Amino acids other than alpha- or beta amino acids, e.g. gamma amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/04—Methods of creating libraries, e.g. combinatorial synthesis using dynamic combinatorial chemistry techniques

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Sustainable Development (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
HK19101116.6A 2015-10-06 2019-01-22 用於生產核酸和蛋白質的裝置和方法 HK1258715A1 (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562237764P 2015-10-06 2015-10-06
US201662334304P 2016-05-10 2016-05-10
PCT/US2016/055292 WO2017062343A1 (en) 2015-10-06 2016-10-04 Devices and methods for producing nucleic acids and proteins

Publications (1)

Publication Number Publication Date
HK1258715A1 true HK1258715A1 (zh) 2019-11-15

Family

ID=57145054

Family Applications (1)

Application Number Title Priority Date Filing Date
HK19101116.6A HK1258715A1 (zh) 2015-10-06 2019-01-22 用於生產核酸和蛋白質的裝置和方法

Country Status (4)

Country Link
US (2) US20180291413A1 (zh)
EP (2) EP3359669B1 (zh)
HK (1) HK1258715A1 (zh)
WO (1) WO2017062343A1 (zh)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110100011A (zh) * 2016-11-21 2019-08-06 诺维信公司 Dna分子的酵母细胞提取物辅助构建
US20210147830A1 (en) * 2018-06-29 2021-05-20 Thermo Fisher Scientific Geneart Gmbh High throughput assembly of nucleic acid molecules
US20210040472A1 (en) * 2019-08-09 2021-02-11 Nutcracker Therapeutics, Inc. Methods and apparatuses for manufacturing for removing material from a therapeutic composition
WO2021178210A1 (en) * 2020-03-03 2021-09-10 Codex Dna, Inc. Methods for assembling nucleic acids
GB202406294D0 (en) 2024-05-06 2024-06-19 Thermo Fisher Scientific Baltics Uab Methods and compositions for producing capped mRNA

Family Cites Families (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4652639A (en) 1982-05-06 1987-03-24 Amgen Manufacture and expression of structural genes
CA2115049C (en) 1991-09-06 2003-10-21 Michael G. Rosenblum Dna sequences encoding gelonin polypeptide
US5869644A (en) 1992-04-15 1999-02-09 The Johns Hopkins University Synthesis of diverse and useful collections of oligonucleotidies
US5580759A (en) 1994-02-03 1996-12-03 Board Of Regents, The University Of Texas System Construction of recombinant DNA by exonuclease recession
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6764835B2 (en) 1995-12-07 2004-07-20 Diversa Corporation Saturation mutageneis in directed evolution
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US6562594B1 (en) 1999-09-29 2003-05-13 Diversa Corporation Saturation mutagenesis in directed evolution
US6495318B2 (en) 1996-06-17 2002-12-17 Vectorobjects, Llc Method and kits for preparing multicomponent nucleic acid constructs
US6110668A (en) 1996-10-07 2000-08-29 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Gene synthesis method
US6153410A (en) 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
AU6846698A (en) 1997-04-01 1998-10-22 Glaxo Group Limited Method of nucleic acid amplification
DE19736591A1 (de) 1997-08-22 1999-02-25 Peter Prof Dr Hegemann Verfahren zum Herstellen von Nukleinsäurepolymeren
ES2340857T3 (es) 1997-09-16 2010-06-10 Centocor Ortho Biotech Inc. Metodo para la sintesis quimica completa y emsamblaje de genes y genomas.
US6083726A (en) 1998-02-03 2000-07-04 Lucent Technologies, Inc. Methods for polynucleotide synthesis and articles for polynucleotide hybridization
EP1112355A1 (en) 1998-09-14 2001-07-04 Aston University Gene and protein libraries and methods relating thereto
US8137906B2 (en) 1999-06-07 2012-03-20 Sloning Biotechnology Gmbh Method for the synthesis of DNA fragments
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
EP1358322B1 (en) 2001-02-02 2009-10-28 Large Scale Biology Corporation A method of increasing complementarity in a heteroduplex polynucleotide
ATE431407T1 (de) 2002-01-11 2009-05-15 Biospring Ges Fuer Biotechnolo Verfahren zur herstellung von dna
US7078211B2 (en) 2002-02-01 2006-07-18 Large Scale Biology Corporation Nucleic acid molecules encoding endonucleases and methods of use thereof
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
GB0213816D0 (en) 2002-06-14 2002-07-24 Univ Aston Method of producing DNA and protein libraries
DE10260805A1 (de) 2002-12-23 2004-07-22 Geneart Gmbh Verfahren und Vorrichtung zum Optimieren einer Nucleotidsequenz zur Expression eines Proteins
JP2007534320A (ja) 2004-02-27 2007-11-29 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ ポリヌクレオチド合成法
US7892725B2 (en) 2004-03-29 2011-02-22 Inguran, Llc Process for storing a sperm dispersion
DE602005026128D1 (de) 2004-08-27 2011-03-10 Wisconsin Alumni Res Found Verfahren zur fehlerverringerung in nukleinsäurepopulationen
WO2006078821A2 (en) * 2005-01-21 2006-07-27 Invitrogen Corporation Products and processes for in vitro synthesis of biomolecules
EP1963525B2 (en) 2005-12-02 2017-10-11 Synthetic Genomics, Inc. Synthesis of error-minimized nucleic acid molecules
US20070231805A1 (en) 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
US20070292954A1 (en) 2006-04-21 2007-12-20 The Brigham And Women's Hospital, Inc. Generation of recombinant DNA by sequence-and ligation-independent cloning
AU2007298650B2 (en) 2006-05-04 2013-10-17 Abbott Diagnostics Scarborough, Inc. Recombinase polymerase amplification
CA2656022C (en) 2006-06-19 2017-10-17 The Johns Hopkins University Single-molecule pcr on microparticles in water-in-oil emulsions
WO2008005675A2 (en) 2006-06-30 2008-01-10 Applera Corporation Emulsion pcr and amplicon capture
DE102006039479A1 (de) 2006-08-23 2008-03-06 Febit Biotech Gmbh Programmierbare Oligonukleotidsynthese
US20110124049A1 (en) 2007-08-07 2011-05-26 Mo-Huang Li Integrated microfluidic device for gene synthesis
US20100291633A1 (en) 2007-09-03 2010-11-18 Thorsten Selmer Method of cloning at least one nucleic acid molecule of interest using type iis restriction endonucleases, and corresponding cloning vectors, kits and system using type iis restriction endonucleases
AU2008311000B2 (en) 2007-10-08 2013-12-19 Synthetic Genomics, Inc. Assembly of large nucleic acids
DK3064599T3 (en) 2008-02-15 2019-04-08 Synthetic Genomics Inc METHODS IN VITRO FOR COMBINING AND COMBINATORY CONSTRUCTION OF NUCLEIC ACID MOLECULES
EP2130918A1 (de) 2008-06-05 2009-12-09 C-Lecta GmbH Verfahren zur Erzeugung einer Varianten-Bibliothek von DNA-Sequenzen
US20090312196A1 (en) 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants
US20100062495A1 (en) 2008-09-10 2010-03-11 Genscript Corporation Homologous recombination-based DNA cloning methods and compositions
EP2175018B1 (en) 2008-10-08 2011-06-08 Icon Genetics GmbH Process of clean cloning
DK2398915T3 (en) 2009-02-20 2016-12-12 Synthetic Genomics Inc Synthesis of nucleic acids sequence verified
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US9309566B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
EP3360974A1 (en) 2009-06-05 2018-08-15 Alere San Diego, Inc. Recombinase polymerase amplification reagents
WO2011102802A1 (en) 2010-02-18 2011-08-25 Agency For Science, Technology And Research Method for reducing mismatches in double-stranded dna molecules
CN110079588B (zh) 2010-12-17 2024-03-15 生命技术公司 用于核酸扩增的方法、组合物、系统、仪器和试剂盒
CA2831140C (en) 2011-04-07 2017-11-07 Alere San Diego, Inc. Monitoring recombinase polymerase amplification mixtures
US9121047B2 (en) 2011-04-07 2015-09-01 Life Technologies Corporation System and methods for making and processing emulsions
US20140155297A1 (en) 2012-02-24 2014-06-05 Cambrian Genomics, Inc. Method and apparatus for light based recovery of sequence verified dna
CN104471075B (zh) 2012-04-19 2018-06-22 生命技术公司 核酸扩增
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US8808989B1 (en) 2013-04-02 2014-08-19 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids

Also Published As

Publication number Publication date
EP3763818A1 (en) 2021-01-13
US20180291413A1 (en) 2018-10-11
WO2017062343A1 (en) 2017-04-13
EP3359669A1 (en) 2018-08-15
EP3359669B1 (en) 2020-05-13
US20220090161A1 (en) 2022-03-24

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