HK1252480A1 - 多聚體蛋白質的純度測定 - Google Patents

多聚體蛋白質的純度測定

Info

Publication number
HK1252480A1
HK1252480A1 HK18111788.3A HK18111788A HK1252480A1 HK 1252480 A1 HK1252480 A1 HK 1252480A1 HK 18111788 A HK18111788 A HK 18111788A HK 1252480 A1 HK1252480 A1 HK 1252480A1
Authority
HK
Hong Kong
Prior art keywords
multimeric protein
protein purity
purity determination
determination
multimeric
Prior art date
Application number
HK18111788.3A
Other languages
English (en)
Inventor
Kathir Muthusamy
Jiann-Kae Luo
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of HK1252480A1 publication Critical patent/HK1252480A1/zh

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/526—CH3 domain
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/061—Sources
    • G01N2201/06113—Coherent sources; lasers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Optics & Photonics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
HK18111788.3A 2015-05-12 2018-09-13 多聚體蛋白質的純度測定 HK1252480A1 (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201562160341P 2015-05-12 2015-05-12

Publications (1)

Publication Number Publication Date
HK1252480A1 true HK1252480A1 (zh) 2019-05-24

Family

ID=56072452

Family Applications (1)

Application Number Title Priority Date Filing Date
HK18111788.3A HK1252480A1 (zh) 2015-05-12 2018-09-13 多聚體蛋白質的純度測定

Country Status (11)

Country Link
US (2) US10520511B2 (zh)
EP (2) EP3294775B1 (zh)
JP (3) JP7240812B2 (zh)
AR (1) AR104610A1 (zh)
AU (2) AU2016261854B2 (zh)
CA (1) CA2984010A1 (zh)
ES (1) ES2881359T3 (zh)
HK (1) HK1252480A1 (zh)
MA (2) MA56416A (zh)
PL (1) PL3294775T3 (zh)
WO (1) WO2016183222A1 (zh)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3384274T3 (da) 2015-11-30 2021-12-13 Intabio Llc Fremgangsmåder til prøvekarakterisering
EP3541365A1 (en) 2016-11-21 2019-09-25 Just Biotherapeutics, Inc. Aflibercept formulations and uses thereof
CA3067735A1 (en) 2017-08-17 2019-02-21 Just Biotherapeutics, Inc. Method of purifying glycosylated protein from host cell galectins and other contaminants
CA3089842A1 (en) 2018-01-29 2019-08-01 Intabio, Inc. Devices, methods and kits for sample characterization
US20190369068A1 (en) 2018-05-31 2019-12-05 Intabio, Inc. Software for microfluidic systems interfacing with mass spectrometry
CN112654639A (zh) * 2018-09-10 2021-04-13 基因泰克公司 用于亲和毛细管电泳的系统和方法
US11573226B2 (en) 2018-09-10 2023-02-07 Genentech, Inc. Systems and methods for affinity capillary electrophoresis
US11782023B2 (en) * 2018-12-19 2023-10-10 Regeneron Pharmaceuticals, Inc. Ce-western applications for antibody development
US11285484B2 (en) 2019-08-12 2022-03-29 Intabio, Llc Multichannel isoelectric focusing devices and high voltage power supplies

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPP385598A0 (en) 1998-06-02 1998-06-25 Life Therapeutics Limited Purification of antibodies
US6818112B2 (en) * 1999-04-20 2004-11-16 Target Discovery, Inc. Protein separation via multidimensional electrophoresis
US7087411B2 (en) 1999-06-08 2006-08-08 Regeneron Pharmaceuticals, Inc. Fusion protein capable of binding VEGF
AUPQ743300A0 (en) * 2000-05-11 2000-06-01 Life Therapeutics Limited Separation of avian antibodies
JP3727317B2 (ja) 2002-03-08 2005-12-14 エイエスエムエル ネザランドズ ベスローテン フエンノートシャップ リソグラフィに使用するためのマスク、マスクを作成する方法、リソグラフィ装置、およびデバイス製造方法
EP1803814A1 (en) * 2005-12-27 2007-07-04 SIGMA-TAU Industrie Farmaceutiche Riunite S.p.A. Method of improving the antibody selection capacity in phage-display library
CN105177091A (zh) * 2006-03-31 2015-12-23 中外制药株式会社 用于纯化双特异性抗体的抗体修饰方法
US7771997B2 (en) 2007-06-04 2010-08-10 Regeneron Pharmaceuticals, Inc. Enhanced expression and stability regions
JP4977588B2 (ja) 2007-12-07 2012-07-18 富士フイルム株式会社 イムノクロマトグラフ方法
CN103833855A (zh) 2009-06-26 2014-06-04 瑞泽恩制药公司 容易地分离的具有天然免疫球蛋白形式的双特异性抗体
WO2012162068A2 (en) * 2011-05-21 2012-11-29 Macrogenics, Inc. Deimmunized serum-binding domains and their use for extending serum half-life
GB2504139B (en) 2012-07-20 2014-12-31 Argen X Bv Antibodies to highly conserved targets produced by the immunisation of Camelidae species
DK2825559T3 (da) 2012-03-13 2019-06-03 Novimmune Sa Letisolerbare bispecifikke antistoffer med nativt immunoglobulinformat
JP2015525071A (ja) 2012-06-05 2015-09-03 リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． 共通の軽鎖を用いて完全ヒト型二重特異性抗体を作製するための方法
TW202206465A (zh) * 2012-11-14 2022-02-16 美商再生元醫藥公司 重組細胞表面捕捉蛋白質
CA2922979A1 (en) * 2013-09-03 2015-03-12 Novimmune S.A. Readily isolated bispecific binding molecules with native format having mutated constant regions

Also Published As

Publication number Publication date
MA56416A (fr) 2022-05-04
ES2881359T3 (es) 2021-11-29
US20200166522A1 (en) 2020-05-28
EP3294775B1 (en) 2021-07-14
JP2018528389A (ja) 2018-09-27
JP7240812B2 (ja) 2023-03-16
AU2016261854A1 (en) 2017-11-09
US20170045527A1 (en) 2017-02-16
US10520511B2 (en) 2019-12-31
PL3294775T3 (pl) 2021-12-13
WO2016183222A1 (en) 2016-11-17
EP3885368A1 (en) 2021-09-29
AU2016261854B2 (en) 2022-05-26
EP3294775A1 (en) 2018-03-21
JP2022171730A (ja) 2022-11-11
AR104610A1 (es) 2017-08-02
CA2984010A1 (en) 2016-11-17
MA43987A (fr) 2018-12-19
WO2016183222A4 (en) 2017-01-05
AU2022203033A1 (en) 2022-05-26
JP2021152544A (ja) 2021-09-30
JP7137663B2 (ja) 2022-09-14

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