HK1252144A1 - 提高原代細胞中基於核酸內切酶的基因編輯 - Google Patents
提高原代細胞中基於核酸內切酶的基因編輯 Download PDFInfo
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- HK1252144A1 HK1252144A1 HK18111535.9A HK18111535A HK1252144A1 HK 1252144 A1 HK1252144 A1 HK 1252144A1 HK 18111535 A HK18111535 A HK 18111535A HK 1252144 A1 HK1252144 A1 HK 1252144A1
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
- C12N7/04—Inactivation or attenuation; Producing viral sub-units
- C12N7/045—Pseudoviral particles; Non infectious pseudovirions, e.g. genetically engineered
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- C12N2310/00—Structure or type of the nucleic acid
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- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
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- Proteomics, Peptides & Aminoacids (AREA)
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- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
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Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562161104P | 2015-05-13 | 2015-05-13 | |
| US62/161,104 | 2015-05-13 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HK1252144A1 true HK1252144A1 (zh) | 2019-05-17 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HK18111535.9A HK1252144A1 (zh) | 2015-05-13 | 2016-05-12 | 提高原代細胞中基於核酸內切酶的基因編輯 |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US10563226B2 (enExample) |
| EP (1) | EP3294343A4 (enExample) |
| JP (1) | JP2018520648A (enExample) |
| CN (1) | CN108136047B (enExample) |
| AU (1) | AU2016262093B2 (enExample) |
| CA (1) | CA2985650A1 (enExample) |
| HK (1) | HK1252144A1 (enExample) |
| WO (1) | WO2016183345A1 (enExample) |
Families Citing this family (76)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US9950282B2 (en) | 2012-03-15 | 2018-04-24 | Flodesign Sonics, Inc. | Electronic configuration and control for acoustic standing wave generation |
| US10967298B2 (en) | 2012-03-15 | 2021-04-06 | Flodesign Sonics, Inc. | Driver and control for variable impedence load |
| US9458450B2 (en) | 2012-03-15 | 2016-10-04 | Flodesign Sonics, Inc. | Acoustophoretic separation technology using multi-dimensional standing waves |
| US10704021B2 (en) | 2012-03-15 | 2020-07-07 | Flodesign Sonics, Inc. | Acoustic perfusion devices |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
| CA2935960C (en) | 2014-01-08 | 2023-01-10 | Bart Lipkens | Acoustophoresis device with dual acoustophoretic chamber |
| AU2015298571B2 (en) | 2014-07-30 | 2020-09-03 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| US11708572B2 (en) | 2015-04-29 | 2023-07-25 | Flodesign Sonics, Inc. | Acoustic cell separation techniques and processes |
| US11021699B2 (en) | 2015-04-29 | 2021-06-01 | FioDesign Sonics, Inc. | Separation using angled acoustic waves |
| US11377651B2 (en) | 2016-10-19 | 2022-07-05 | Flodesign Sonics, Inc. | Cell therapy processes utilizing acoustophoresis |
| US11459540B2 (en) | 2015-07-28 | 2022-10-04 | Flodesign Sonics, Inc. | Expanded bed affinity selection |
| US11474085B2 (en) | 2015-07-28 | 2022-10-18 | Flodesign Sonics, Inc. | Expanded bed affinity selection |
| GB2557123B (en) | 2015-07-31 | 2021-11-03 | Univ Minnesota | Modified cells and methods of therapy |
| US12043852B2 (en) | 2015-10-23 | 2024-07-23 | President And Fellows Of Harvard College | Evolved Cas9 proteins for gene editing |
| WO2017184674A1 (en) * | 2016-04-19 | 2017-10-26 | President And Fellows Of Harvard College | Methods for increasing efficiency of nuclease-mediated gene editing in stem cells |
| US11214789B2 (en) | 2016-05-03 | 2022-01-04 | Flodesign Sonics, Inc. | Concentration and washing of particles with acoustics |
| US11085035B2 (en) | 2016-05-03 | 2021-08-10 | Flodesign Sonics, Inc. | Therapeutic cell washing, concentration, and separation utilizing acoustophoresis |
| KR20250103795A (ko) | 2016-08-03 | 2025-07-07 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| CN109804066A (zh) | 2016-08-09 | 2019-05-24 | 哈佛大学的校长及成员们 | 可编程cas9-重组酶融合蛋白及其用途 |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| AU2017342543B2 (en) | 2016-10-14 | 2024-06-27 | President And Fellows Of Harvard College | AAV delivery of nucleobase editors |
| CN110520530A (zh) | 2016-10-18 | 2019-11-29 | 明尼苏达大学董事会 | 肿瘤浸润性淋巴细胞和治疗方法 |
| CN110494543A (zh) | 2016-10-19 | 2019-11-22 | 弗洛设计声能学公司 | 通过声学的亲和细胞提取 |
| WO2018081476A2 (en) * | 2016-10-27 | 2018-05-03 | Intima Bioscience, Inc. | Viral methods of t cell therapy |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| CN107746845B (zh) * | 2016-12-28 | 2021-03-26 | 北京微旋基因技术有限公司 | 特异性靶向LAG-3基因的sgRNA和特异性敲除LAG-3基因的方法 |
| CN107760680B (zh) * | 2016-12-28 | 2020-09-22 | 北京微旋基因技术有限公司 | 特异性靶向TIM-3基因的sgRNA和特异性敲除TIM-3基因的方法 |
| EP3592381A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Cancer vaccine |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| KR20190127797A (ko) | 2017-03-10 | 2019-11-13 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 시토신에서 구아닌으로의 염기 편집제 |
| CA3057192A1 (en) | 2017-03-23 | 2018-09-27 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| CN108866100A (zh) * | 2017-05-16 | 2018-11-23 | 中国科学院上海生命科学研究院 | 一种高效率的基因编辑方法 |
| WO2019006418A2 (en) | 2017-06-30 | 2019-01-03 | Intima Bioscience, Inc. | ADENO-ASSOCIATED VIRAL VECTORS FOR GENE THERAPY |
| JP2020530277A (ja) | 2017-06-30 | 2020-10-22 | セレクティスCellectis | 反復投与のための細胞免疫療法 |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
| CA3082251A1 (en) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | Uses of adenosine base editors |
| WO2019084168A1 (en) * | 2017-10-24 | 2019-05-02 | Editas Medicine, Inc. | SYSTEMS AND METHODS FOR TREATING HYPER-IGM SYNDROME |
| KR20220066413A (ko) | 2017-12-14 | 2022-05-24 | 프로디자인 소닉스, 인크. | 음향 트랜스듀서 구동기 및 제어기 |
| EP3724214A4 (en) | 2017-12-15 | 2021-09-01 | The Broad Institute Inc. | SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING |
| BR112020018658A2 (pt) | 2018-03-15 | 2020-12-29 | KSQ Therapeutics, Inc. | Composições de regulação gênica e métodos para imu-noterapia aprimorada |
| US20210071202A1 (en) * | 2018-03-29 | 2021-03-11 | Jichi Medical University | Genome editing method, composition, cell, cell preparation, and method for producing cell preparation |
| EP3784292A4 (en) * | 2018-04-27 | 2022-01-19 | Seattle Children's Hospital (DBA Seattle Children's Research Institute) | THERAPEUTIC GENOME EDITING IN HYPER-IGM SEX-LINKED SYNDROME |
| KR20210045360A (ko) | 2018-05-16 | 2021-04-26 | 신테고 코포레이션 | 가이드 rna 설계 및 사용을 위한 방법 및 시스템 |
| WO2019226953A1 (en) | 2018-05-23 | 2019-11-28 | The Broad Institute, Inc. | Base editors and uses thereof |
| CA3101477A1 (en) * | 2018-05-30 | 2019-12-05 | M2X2 Therapeutics, Inc. | Cell therapy |
| EP3810638A1 (en) * | 2018-06-25 | 2021-04-28 | Ospedale San Raffaele S.r.l. | Gene therapy |
| US12281338B2 (en) | 2018-10-29 | 2025-04-22 | The Broad Institute, Inc. | Nucleobase editors comprising GeoCas9 and uses thereof |
| CN109528653B (zh) * | 2018-11-22 | 2020-07-31 | 北京大学 | 具有基因编辑功能的膜性囊泡及其制备方法、药物组合物和用途 |
| CN109735516B (zh) * | 2019-01-22 | 2021-09-03 | 自然资源部第一海洋研究所 | 受核苷酸片段引导具有特异核酸内切酶活性的piwi蛋白 |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| DE112020001306T5 (de) | 2019-03-19 | 2022-01-27 | Massachusetts Institute Of Technology | Verfahren und zusammensetzungen zur editierung von nukleotidsequenzen |
| CN111812066B (zh) * | 2019-04-10 | 2023-04-28 | 华东理工大学 | 基于CRISPR/Cas12a系统的生物传感器、试剂盒及其在小分子检测中的用途 |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| JP2023525304A (ja) | 2020-05-08 | 2023-06-15 | ザ ブロード インスティテュート,インコーポレーテッド | 標的二本鎖ヌクレオチド配列の両鎖同時編集のための方法および組成物 |
| CN111909961B (zh) * | 2020-08-21 | 2022-04-19 | 华侨大学 | 一种CRISPR/Cas介导的ATL细胞基因编辑载体及其应用 |
| EP4262850A4 (en) * | 2020-12-21 | 2025-07-09 | Novo Nordisk As | COMPOSITIONS AND METHODS FOR SITE-DIRECTED MUTAGENESIS |
| EP4288088A2 (en) * | 2021-02-08 | 2023-12-13 | Intellia Therapeutics, Inc. | Lymphocyte activation gene 3 (lag3) compositions and methods for immunotherapy |
| WO2022170193A2 (en) * | 2021-02-08 | 2022-08-11 | Intellia Therapeutics, Inc. | T-cell immunoglobulin and mucin domain 3 (tim3) compositions and methods for immunotherapy |
| CN113481184A (zh) * | 2021-08-06 | 2021-10-08 | 北京大学 | 融合蛋白以及其使用方法 |
| JP2024534114A (ja) * | 2021-08-24 | 2024-09-18 | インテリア セラピューティクス,インコーポレイテッド | 細胞療法用のプログラム細胞死タンパク質1(pd1)組成物及び方法 |
| JP2024543216A (ja) * | 2021-12-03 | 2024-11-19 | ザ ジェネラル ホスピタル コーポレイション | Crispr関連トランスポザーゼおよびその使用方法 |
| WO2023122036A1 (en) * | 2021-12-20 | 2023-06-29 | Sobol Robert E | Methods and compositions for improved molecular therapies of multigenic diseases |
| JP2025531296A (ja) * | 2022-09-19 | 2025-09-19 | エメンドバイオ・インコーポレイテッド | Pdcd1の両アレルノックアウト |
| WO2024102954A1 (en) | 2022-11-10 | 2024-05-16 | Massachusetts Institute Of Technology | Activation induced clipping system (aics) |
| WO2024107119A1 (en) * | 2022-11-17 | 2024-05-23 | Agency For Science, Technology And Research | T3 vectors for recombinant protein production in mammalian cells |
| CN117987436B (zh) * | 2024-04-03 | 2024-06-25 | 南京鸿明生物科技有限公司 | 一种双链靶dna序列的制备方法 |
| WO2025235862A1 (en) | 2024-05-10 | 2025-11-13 | Inndura Therapeutics Inc. | A modified immune cell receptor comprising a target-binding domain and the extracellular domain of cd16a |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2415333A1 (en) * | 2000-08-03 | 2002-02-14 | Onyx Pharmaceuticals, Inc. | Adenovirus e1b-55k single amino acid mutants and methods of use |
| US7838219B2 (en) | 2001-02-02 | 2010-11-23 | Novici Biotech Llc | Method of increasing complementarity in a heteroduplex |
| EP1590468A2 (en) | 2003-01-28 | 2005-11-02 | Cellectis | Use of meganucleases for inducing homologous recombination ex vivo and in toto in vertebrate somatic tissues and application thereof |
| EP1591521A1 (en) | 2004-04-30 | 2005-11-02 | Cellectis | I-Dmo I derivatives with enhanced activity at 37 degrees C and use thereof |
| WO2008153421A1 (en) * | 2007-06-15 | 2008-12-18 | Otago Innovation Limited | Anticancer therapies |
| EP2412806B1 (en) | 2010-07-28 | 2014-01-08 | Institut Pasteur | Use of terminal deoxynucleotidyl transferase for mutagenic DNA repair to generate variability, at a determined position in DNA |
| JP2013544082A (ja) | 2010-10-27 | 2013-12-12 | セレクティス | 二本鎖破断−誘導変異誘発の効率を増大させる方法 |
| US9044492B2 (en) | 2011-02-04 | 2015-06-02 | Cellectis Sa | Method for modulating the efficiency of double-strand break-induced mutagenesis |
| EP2729567B1 (en) | 2011-07-08 | 2016-10-05 | Cellectis | Method for increasing the efficiency of double-strand break-induced mutagenssis |
| US20130129668A1 (en) * | 2011-09-01 | 2013-05-23 | The Regents Of The University Of California | Diagnosis and treatment of arthritis using epigenetics |
| EP2931898B1 (en) * | 2012-12-12 | 2016-03-09 | The Broad Institute, Inc. | Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains |
| US9234213B2 (en) | 2013-03-15 | 2016-01-12 | System Biosciences, Llc | Compositions and methods directed to CRISPR/Cas genomic engineering systems |
| US11274305B2 (en) | 2013-04-04 | 2022-03-15 | Trustees Of Dartmouth College | Compositions and methods for in vivo excision of HIV-1 proviral DNA |
| AU2014312123A1 (en) | 2013-08-29 | 2016-03-17 | Temple University Of The Commonwealth System Of Higher Education | Methods and compositions for RNA-guided treatment of HIV infection |
| US20160237455A1 (en) * | 2013-09-27 | 2016-08-18 | Editas Medicine, Inc. | Crispr-related methods and compositions |
| US9957526B2 (en) * | 2013-10-17 | 2018-05-01 | Sangamo Therapeutics, Inc. | Delivery methods and compositions for nuclease-mediated genome engineering |
| ES3015369T3 (en) * | 2014-10-09 | 2025-05-05 | Seattle Childrens Hospital Dba Seattle Childrens Res Inst | Long poly (a) plasmids and methods for introduction of long poly (a) sequences into the plasmid |
| EP3256170B1 (en) * | 2015-02-13 | 2020-09-23 | University of Massachusetts | Compositions and methods for transient delivery of nucleases |
-
2016
- 2016-05-12 US US15/152,960 patent/US10563226B2/en active Active
- 2016-05-12 WO PCT/US2016/032153 patent/WO2016183345A1/en not_active Ceased
- 2016-05-12 CN CN201680039989.4A patent/CN108136047B/zh active Active
- 2016-05-12 AU AU2016262093A patent/AU2016262093B2/en not_active Ceased
- 2016-05-12 CA CA2985650A patent/CA2985650A1/en active Pending
- 2016-05-12 EP EP16793541.0A patent/EP3294343A4/en not_active Withdrawn
- 2016-05-12 JP JP2017559075A patent/JP2018520648A/ja not_active Ceased
- 2016-05-12 HK HK18111535.9A patent/HK1252144A1/zh unknown
- 2016-05-12 US US15/572,761 patent/US20180119174A1/en not_active Abandoned
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2020
- 2020-01-13 US US16/740,995 patent/US20200377911A1/en not_active Abandoned
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| Publication number | Publication date |
|---|---|
| AU2016262093A1 (en) | 2017-11-30 |
| AU2016262093B2 (en) | 2022-08-18 |
| AU2016262093A8 (en) | 2017-12-07 |
| CA2985650A1 (en) | 2016-11-17 |
| US10563226B2 (en) | 2020-02-18 |
| US20160333377A1 (en) | 2016-11-17 |
| WO2016183345A1 (en) | 2016-11-17 |
| EP3294343A1 (en) | 2018-03-21 |
| CN108136047A (zh) | 2018-06-08 |
| CN108136047B (zh) | 2022-12-13 |
| US20180119174A1 (en) | 2018-05-03 |
| EP3294343A4 (en) | 2018-09-26 |
| US20200377911A1 (en) | 2020-12-03 |
| JP2018520648A (ja) | 2018-08-02 |
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