HK1202896A1 - A prokaryote-based cell-free system for the synthesis of glycoproteins - Google Patents

A prokaryote-based cell-free system for the synthesis of glycoproteins Download PDF

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Publication number
HK1202896A1
HK1202896A1 HK15103270.8A HK15103270A HK1202896A1 HK 1202896 A1 HK1202896 A1 HK 1202896A1 HK 15103270 A HK15103270 A HK 15103270A HK 1202896 A1 HK1202896 A1 HK 1202896A1
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HK
Hong Kong
Prior art keywords
glycans
glycan
amino acid
glycoprotein
eukaryotic
Prior art date
Application number
HK15103270.8A
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English (en)
Chinese (zh)
Inventor
．德利薩
M.德利萨
Original Assignee
康奈尔大学
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Publication date
Application filed by 康奈尔大学 filed Critical 康奈尔大学
Publication of HK1202896A1 publication Critical patent/HK1202896A1/xx

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
HK15103270.8A 2011-11-04 2012-11-05 A prokaryote-based cell-free system for the synthesis of glycoproteins HK1202896A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161555854P 2011-11-04 2011-11-04
US61/555,854 2011-11-04
PCT/US2012/063590 WO2013067523A1 (en) 2011-11-04 2012-11-05 A prokaryote-based cell-free system for the synthesis of glycoproteins

Publications (1)

Publication Number Publication Date
HK1202896A1 true HK1202896A1 (en) 2015-10-09

Family

ID=48192910

Family Applications (1)

Application Number Title Priority Date Filing Date
HK15103270.8A HK1202896A1 (en) 2011-11-04 2012-11-05 A prokaryote-based cell-free system for the synthesis of glycoproteins

Country Status (5)

Country Link
US (2) US11193154B2 (enExample)
CN (2) CN104080921A (enExample)
HK (1) HK1202896A1 (enExample)
IN (1) IN2014CN04076A (enExample)
WO (1) WO2013067523A1 (enExample)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20160021075A (ko) * 2013-03-14 2016-02-24 글리코비아, 인크. 올리고사카라이드 조성물, 당단백질, 및 원핵생물에서의 이들의 제조 방법
CN106232832B (zh) 2013-12-06 2022-04-05 哈佛大学校长及研究员协会 基于纸的合成基因网络
US20170198326A1 (en) * 2014-07-08 2017-07-13 Technion Research & Development Foundation Limited Methods and kits for cell-free transcription and translation
SI3240895T1 (sl) * 2014-12-30 2022-04-29 Glaxosmithkline Biologicals S.A. Sestavki in postopki za glikozilacijo proteinov
US10265391B2 (en) 2015-02-26 2019-04-23 Vaxnewmo Llc Acinetobacter O-oligosaccharyltransferases and uses thereof
CN106478773B (zh) * 2015-08-25 2021-09-14 三生国健药业（上海）股份有限公司 一种人工合成的新型信号肽
WO2017117539A1 (en) * 2015-12-30 2017-07-06 Northwestern University Cell-free glycoprotein synthesis (cfgps) in prokaryotic cell lysates enriched with components for glycosylation
US10829795B2 (en) 2016-07-14 2020-11-10 Northwestern University Method for rapid in vitro synthesis of glycoproteins via recombinant production of N-glycosylated proteins in prokaryotic cell lysates
US11898187B2 (en) 2017-08-15 2024-02-13 Northwestern University Protein glycosylation sites by rapid expression and characterization of N-glycosyltransferases
US11530432B2 (en) 2018-03-19 2022-12-20 Northwestern University Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates
WO2019204346A1 (en) 2018-04-16 2019-10-24 Northwestern University METHODS FOR CO-ACTIVATING IN VITRO NON-STANDARD AMINO ACID (nsAA) INCORPORATION AND GLYCOSYLATION IN CRUDE CELLLYSATES
ES3051337T3 (en) 2018-06-16 2025-12-26 Vaxnewmo Llc Glycosylated comp pilin variants, methods of making and uses thereof
US12350342B2 (en) 2018-12-21 2025-07-08 Vaxnewmo Llc O-linked glycosylation recognition motifs
EP3908669A4 (en) 2019-01-11 2023-05-03 Northwestern University SYNTHESIS OF BIOCONJUGATED VACCINES IN PROKARYOT CELL LYSATE
MX2022001606A (es) * 2019-08-09 2022-03-11 Glaxosmithkline Biologicals Sa Enzimas de oligosacariltransferasa pglb mutada.
US12226410B2 (en) 2019-10-18 2025-02-18 Northwestern University Methods for enhancing cellular clearance of pathological molecules via activation of the cellular protein ykt6
WO2021081485A1 (en) 2019-10-25 2021-04-29 Northwestern University Cell-free extract preparation protocol for enrichment of membrane vesicles and increased glycoprotein yields
CN117616124A (zh) * 2021-05-21 2024-02-27 迦西勒斯医药公司 嵌合型气体囊泡及其蛋白质表现系统
CN115181752B (zh) * 2022-07-12 2024-12-24 大连大学 一种糖链质粒优化提高修饰蛋白质效率以及蛋白表达量的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4061043B2 (ja) 2000-12-28 2008-03-12 株式会社ポストゲノム研究所 ｉｎｖｉｔｒｏ転写／翻訳系によるペプチド等の製造方法
DK1481057T3 (da) * 2002-03-07 2006-05-15 Eidgenoess Tech Hochschule System og fremgangsmåde til fremstilling af rekombinante glycosylerede proteiner i en prokaryot vært
JP4590249B2 (ja) * 2004-11-17 2010-12-01 独立行政法人理化学研究所 糖タンパク質合成用の無細胞タンパク質合成システム
WO2009089154A2 (en) 2008-01-03 2009-07-16 Cornell Research Foundation, Inc. Glycosylated protein expression in prokaryotes
CN102037004A (zh) * 2008-01-08 2011-04-27 生物种属学股份公司 使用寡糖基转移酶的多肽的糖缀合

Also Published As

Publication number Publication date
US11193154B2 (en) 2021-12-07
WO2013067523A1 (en) 2013-05-10
US20140255987A1 (en) 2014-09-11
CN104080921A (zh) 2014-10-01
US20220340947A1 (en) 2022-10-27
CN112980907A (zh) 2021-06-18
IN2014CN04076A (enExample) 2015-10-23

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