HK1202896A1 - 種用於糖蛋白合成的基於原核生物的無細胞系統 - Google Patents

種用於糖蛋白合成的基於原核生物的無細胞系統

Info

Publication number
HK1202896A1
HK1202896A1 HK15103270.8A HK15103270A HK1202896A1 HK 1202896 A1 HK1202896 A1 HK 1202896A1 HK 15103270 A HK15103270 A HK 15103270A HK 1202896 A1 HK1202896 A1 HK 1202896A1
Authority
HK
Hong Kong
Prior art keywords
prokaryote
glycoproteins
synthesis
free system
based cell
Prior art date
Application number
HK15103270.8A
Other languages
English (en)
Inventor
．德利薩
Original Assignee
Univ Cornell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Cornell filed Critical Univ Cornell
Publication of HK1202896A1 publication Critical patent/HK1202896A1/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
HK15103270.8A 2011-11-04 2015-03-31 種用於糖蛋白合成的基於原核生物的無細胞系統 HK1202896A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161555854P 2011-11-04 2011-11-04
PCT/US2012/063590 WO2013067523A1 (en) 2011-11-04 2012-11-05 A prokaryote-based cell-free system for the synthesis of glycoproteins

Publications (1)

Publication Number Publication Date
HK1202896A1 true HK1202896A1 (zh) 2015-10-09

Family

ID=48192910

Family Applications (1)

Application Number Title Priority Date Filing Date
HK15103270.8A HK1202896A1 (zh) 2011-11-04 2015-03-31 種用於糖蛋白合成的基於原核生物的無細胞系統

Country Status (5)

Country Link
US (2) US11193154B2 (zh)
CN (2) CN104080921A (zh)
HK (1) HK1202896A1 (zh)
IN (1) IN2014CN04076A (zh)
WO (1) WO2013067523A1 (zh)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201508347YA (en) * 2013-03-14 2015-11-27 Glycobia Inc Oligosaccharide compositions, glycoproteins and methods to produce the same in prokaryotes
US20160312312A1 (en) 2013-12-06 2016-10-27 President And Fellows Of Harvard College Paper-based synthetic gene networks
US20170198326A1 (en) * 2014-07-08 2017-07-13 Technion Research & Development Foundation Limited Methods and kits for cell-free transcription and translation
PT3240895T (pt) * 2014-12-30 2022-03-02 Glaxosmithkline Biologicals Sa Composições e métodos para glicosilação de proteínas
US10265391B2 (en) 2015-02-26 2019-04-23 Vaxnewmo Llc Acinetobacter O-oligosaccharyltransferases and uses thereof
CN106478773B (zh) * 2015-08-25 2021-09-14 三生国健药业（上海）股份有限公司 一种人工合成的新型信号肽
WO2017117539A1 (en) 2015-12-30 2017-07-06 Northwestern University Cell-free glycoprotein synthesis (cfgps) in prokaryotic cell lysates enriched with components for glycosylation
US10829795B2 (en) 2016-07-14 2020-11-10 Northwestern University Method for rapid in vitro synthesis of glycoproteins via recombinant production of N-glycosylated proteins in prokaryotic cell lysates
US11898187B2 (en) 2017-08-15 2024-02-13 Northwestern University Protein glycosylation sites by rapid expression and characterization of N-glycosyltransferases
US11530432B2 (en) 2018-03-19 2022-12-20 Northwestern University Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates
US11725224B2 (en) 2018-04-16 2023-08-15 Northwestern University Methods for co-activating in vitro non-standard amino acid (nsAA) incorporation and glycosylation in crude cell lysates
US11932670B2 (en) 2018-06-16 2024-03-19 Vaxnewmo Llc Glycosylated ComP pilin variants, methods of making and uses thereof
CN115181752A (zh) * 2022-07-12 2022-10-14 大连大学 一种糖链质粒优化提高修饰蛋白质效率以及蛋白表达量的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4061043B2 (ja) * 2000-12-28 2008-03-12 株式会社ポストゲノム研究所 ｉｎｖｉｔｒｏ転写／翻訳系によるペプチド等の製造方法
EP1481057B1 (en) 2002-03-07 2006-02-15 Eidgenössische Technische Hochschule Zürich System and method for the production of recombinant glycosylated proteins in a prokaryotic host
JP4590249B2 (ja) * 2004-11-17 2010-12-01 独立行政法人理化学研究所 糖タンパク質合成用の無細胞タンパク質合成システム
CN101960017B (zh) * 2008-01-03 2017-06-09 康乃尔研究基金会有限公司 原核生物中的糖基化蛋白表达
US20100286067A1 (en) * 2008-01-08 2010-11-11 Biogenerix Ag Glycoconjugation of polypeptides using oligosaccharyltransferases

Also Published As

Publication number Publication date
IN2014CN04076A (zh) 2015-10-23
CN112980907A (zh) 2021-06-18
US20140255987A1 (en) 2014-09-11
CN104080921A (zh) 2014-10-01
US20220340947A1 (en) 2022-10-27
WO2013067523A1 (en) 2013-05-10
US11193154B2 (en) 2021-12-07

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