IN2014CN04076A - - Google Patents
Info
- Publication number
- IN2014CN04076A IN2014CN04076A IN4076CHN2014A IN2014CN04076A IN 2014CN04076 A IN2014CN04076 A IN 2014CN04076A IN 4076CHN2014 A IN4076CHN2014 A IN 4076CHN2014A IN 2014CN04076 A IN2014CN04076 A IN 2014CN04076A
- Authority
- IN
- India
- Prior art keywords
- glycan
- producing
- glycoprotein target
- cell free
- isolated
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161555854P | 2011-11-04 | 2011-11-04 | |
| PCT/US2012/063590 WO2013067523A1 (en) | 2011-11-04 | 2012-11-05 | A prokaryote-based cell-free system for the synthesis of glycoproteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IN2014CN04076A true IN2014CN04076A (enExample) | 2015-10-23 |
Family
ID=48192910
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IN4076CHN2014 IN2014CN04076A (enExample) | 2011-11-04 | 2012-11-05 |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US11193154B2 (enExample) |
| CN (2) | CN104080921A (enExample) |
| HK (1) | HK1202896A1 (enExample) |
| IN (1) | IN2014CN04076A (enExample) |
| WO (1) | WO2013067523A1 (enExample) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20160021075A (ko) * | 2013-03-14 | 2016-02-24 | 글리코비아, 인크. | 올리고사카라이드 조성물, 당단백질, 및 원핵생물에서의 이들의 제조 방법 |
| CN106232832B (zh) | 2013-12-06 | 2022-04-05 | 哈佛大学校长及研究员协会 | 基于纸的合成基因网络 |
| US20170198326A1 (en) * | 2014-07-08 | 2017-07-13 | Technion Research & Development Foundation Limited | Methods and kits for cell-free transcription and translation |
| SI3240895T1 (sl) * | 2014-12-30 | 2022-04-29 | Glaxosmithkline Biologicals S.A. | Sestavki in postopki za glikozilacijo proteinov |
| US10265391B2 (en) | 2015-02-26 | 2019-04-23 | Vaxnewmo Llc | Acinetobacter O-oligosaccharyltransferases and uses thereof |
| CN106478773B (zh) * | 2015-08-25 | 2021-09-14 | 三生国健药业（上海）股份有限公司 | 一种人工合成的新型信号肽 |
| WO2017117539A1 (en) * | 2015-12-30 | 2017-07-06 | Northwestern University | Cell-free glycoprotein synthesis (cfgps) in prokaryotic cell lysates enriched with components for glycosylation |
| US10829795B2 (en) | 2016-07-14 | 2020-11-10 | Northwestern University | Method for rapid in vitro synthesis of glycoproteins via recombinant production of N-glycosylated proteins in prokaryotic cell lysates |
| US11898187B2 (en) | 2017-08-15 | 2024-02-13 | Northwestern University | Protein glycosylation sites by rapid expression and characterization of N-glycosyltransferases |
| US11530432B2 (en) | 2018-03-19 | 2022-12-20 | Northwestern University | Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates |
| WO2019204346A1 (en) | 2018-04-16 | 2019-10-24 | Northwestern University | METHODS FOR CO-ACTIVATING IN VITRO NON-STANDARD AMINO ACID (nsAA) INCORPORATION AND GLYCOSYLATION IN CRUDE CELLLYSATES |
| ES3051337T3 (en) | 2018-06-16 | 2025-12-26 | Vaxnewmo Llc | Glycosylated comp pilin variants, methods of making and uses thereof |
| US12350342B2 (en) | 2018-12-21 | 2025-07-08 | Vaxnewmo Llc | O-linked glycosylation recognition motifs |
| EP3908669A4 (en) | 2019-01-11 | 2023-05-03 | Northwestern University | SYNTHESIS OF BIOCONJUGATED VACCINES IN PROKARYOT CELL LYSATE |
| MX2022001606A (es) * | 2019-08-09 | 2022-03-11 | Glaxosmithkline Biologicals Sa | Enzimas de oligosacariltransferasa pglb mutada. |
| US12226410B2 (en) | 2019-10-18 | 2025-02-18 | Northwestern University | Methods for enhancing cellular clearance of pathological molecules via activation of the cellular protein ykt6 |
| WO2021081485A1 (en) | 2019-10-25 | 2021-04-29 | Northwestern University | Cell-free extract preparation protocol for enrichment of membrane vesicles and increased glycoprotein yields |
| CN117616124A (zh) * | 2021-05-21 | 2024-02-27 | 迦西勒斯医药公司 | 嵌合型气体囊泡及其蛋白质表现系统 |
| CN115181752B (zh) * | 2022-07-12 | 2024-12-24 | 大连大学 | 一种糖链质粒优化提高修饰蛋白质效率以及蛋白表达量的方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4061043B2 (ja) | 2000-12-28 | 2008-03-12 | 株式会社ポストゲノム研究所 | ｉｎｖｉｔｒｏ転写／翻訳系によるペプチド等の製造方法 |
| DK1481057T3 (da) * | 2002-03-07 | 2006-05-15 | Eidgenoess Tech Hochschule | System og fremgangsmåde til fremstilling af rekombinante glycosylerede proteiner i en prokaryot vært |
| JP4590249B2 (ja) * | 2004-11-17 | 2010-12-01 | 独立行政法人理化学研究所 | 糖タンパク質合成用の無細胞タンパク質合成システム |
| WO2009089154A2 (en) | 2008-01-03 | 2009-07-16 | Cornell Research Foundation, Inc. | Glycosylated protein expression in prokaryotes |
| CN102037004A (zh) * | 2008-01-08 | 2011-04-27 | 生物种属学股份公司 | 使用寡糖基转移酶的多肽的糖缀合 |
-
2012
- 2012-11-05 IN IN4076CHN2014 patent/IN2014CN04076A/en unknown
- 2012-11-05 CN CN201280066129.1A patent/CN104080921A/zh active Pending
- 2012-11-05 WO PCT/US2012/063590 patent/WO2013067523A1/en not_active Ceased
- 2012-11-05 US US14/356,258 patent/US11193154B2/en active Active
- 2012-11-05 CN CN202110180948.XA patent/CN112980907A/zh active Pending
- 2012-11-05 HK HK15103270.8A patent/HK1202896A1/xx unknown
-
2021
- 2021-12-06 US US17/543,614 patent/US20220340947A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US11193154B2 (en) | 2021-12-07 |
| WO2013067523A1 (en) | 2013-05-10 |
| US20140255987A1 (en) | 2014-09-11 |
| CN104080921A (zh) | 2014-10-01 |
| US20220340947A1 (en) | 2022-10-27 |
| HK1202896A1 (en) | 2015-10-09 |
| CN112980907A (zh) | 2021-06-18 |