HK1200912A1 - 精子染色和分選方法 - Google Patents

精子染色和分選方法

Info

Publication number
HK1200912A1
HK1200912A1 HK15101291.7A HK15101291A HK1200912A1 HK 1200912 A1 HK1200912 A1 HK 1200912A1 HK 15101291 A HK15101291 A HK 15101291A HK 1200912 A1 HK1200912 A1 HK 1200912A1
Authority
HK
Hong Kong
Prior art keywords
sorting methods
sperm staining
sperm
staining
sorting
Prior art date
Application number
HK15101291.7A
Other languages
English (en)
Inventor
Kenneth Michael Evans
Thomas B Gilligan
Clara Gonzalez-Marin
Original Assignee
Inguran Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inguran Llc filed Critical Inguran Llc
Publication of HK1200912A1 publication Critical patent/HK1200912A1/zh

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/061—Sperm cells, spermatogonia
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432—Quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
HK15101291.7A 2011-09-30 2015-02-05 精子染色和分選方法 HK1200912A1 (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161541451P 2011-09-30 2011-09-30
US201161541438P 2011-09-30 2011-09-30
PCT/US2012/058008 WO2013049631A1 (en) 2011-09-30 2012-09-28 Sperm staining and sorting methods

Publications (1)

Publication Number Publication Date
HK1200912A1 true HK1200912A1 (zh) 2015-08-14

Family

ID=47046868

Family Applications (1)

Application Number Title Priority Date Filing Date
HK15101291.7A HK1200912A1 (zh) 2011-09-30 2015-02-05 精子染色和分選方法

Country Status (5)

Country Link
US (2) US9581587B2 (zh)
EP (1) EP2761275B1 (zh)
DK (1) DK2761275T3 (zh)
HK (1) HK1200912A1 (zh)
WO (1) WO2013049631A1 (zh)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013049631A1 (en) 2011-09-30 2013-04-04 Inguran, Llc Sperm staining and sorting methods
US10620213B2 (en) 2012-10-05 2020-04-14 Inguran, Llc High pressure sperm sorting and flow cytometer methods
CN103525760B (zh) * 2013-09-29 2016-09-21 大连金弘基种畜有限公司 性控精液的分离方法
WO2016081864A1 (en) * 2014-11-20 2016-05-26 Inguran, Llc Low sugar sperm media and compositions
WO2016118557A1 (en) * 2015-01-20 2016-07-28 President And Fellows Of Harvard College Assessing metabolic condition of sperm
EP3570853B1 (en) * 2017-01-20 2024-07-24 Inguran, LLC Sperm processing method, apparatus and related media compositions
WO2019199852A1 (en) 2018-04-09 2019-10-17 Inguran, Llc Improved sperm nuclei and methods of their manufacture and use
CN109738412B (zh) * 2019-03-05 2021-07-13 贵州大学 一种饮用水中铝、铁、铬、汞、铕、铥和镱离子的检测方法
CN117965432B (zh) * 2024-03-29 2024-06-14 内蒙古大学 一种通过nad+提高牛精子分选后活力的方法

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US139497A (en) 1873-06-03 Improvement in washing-machines
US4559309A (en) 1982-09-01 1985-12-17 Memorial Sloan Kettering Cancer Center Flow cytometry-fluorescence measurements for characterizing sperm
JP2552582B2 (ja) 1989-05-10 1996-11-13 アメリカ合衆国 子の性の前選択の方法
US5895922A (en) 1996-03-19 1999-04-20 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of National Defence Fluorescent biological particle detection system
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US7070917B1 (en) * 1999-03-05 2006-07-04 Preben Christensen Determination of sperm concentration and viability for artificial insemination
US7208265B1 (en) * 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
US7019129B1 (en) 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
AU1980202A (en) 2000-11-22 2002-06-03 Pharmacia Corporation Methods and apparatus for producing gender enriched sperm
US20060067916A1 (en) 2002-07-22 2006-03-30 Xy, Inc. Sperm cell process system
JP2006525019A (ja) 2003-03-28 2006-11-09 モンサント テクノロジー エルエルシー 精子の染色方法
EP2305172B1 (en) 2003-03-28 2016-05-11 Inguran, LLC Apparatus and methods for providing sex-sorted animal sperm
CN101238369B (zh) 2004-03-29 2012-07-04 英格朗公司 调节细胞内和/或细胞外氧化/还原反应的组合物在精子染色或分选方法中的用途
DE602005010515D1 (de) 2004-08-06 2008-12-04 Fuji Electric Holdings Nachweisverfahren für lebenfähige Zellen
BRPI0814343A2 (pt) * 2007-07-23 2017-10-03 Androgenix Ltd Materiais e métodos para a seleção da sexagem do esperma
GB0910826D0 (en) * 2009-06-23 2009-08-05 Ovasort Ltd Identification of sex-linked proteins
CA2778245A1 (en) * 2009-10-30 2011-05-05 Inguran, LLC. Methods and systems for reducing dna fragmentation in a population of sperm cells
CN102812123A (zh) 2010-04-01 2012-12-05 英格朗公司 减少经处理的精子样品中的dna断裂的方法和系统
WO2013049631A1 (en) 2011-09-30 2013-04-04 Inguran, Llc Sperm staining and sorting methods
EP3492603B1 (en) 2012-10-05 2022-07-20 Inguran, LLC Methods of processing sperm for sex sorting
US10620213B2 (en) 2012-10-05 2020-04-14 Inguran, Llc High pressure sperm sorting and flow cytometer methods

Also Published As

Publication number Publication date
US20130084558A1 (en) 2013-04-04
US9581587B2 (en) 2017-02-28
US20140234833A1 (en) 2014-08-21
DK2761275T3 (en) 2017-09-25
US9140688B2 (en) 2015-09-22
EP2761275B1 (en) 2017-06-21
WO2013049631A1 (en) 2013-04-04
EP2761275A1 (en) 2014-08-06
US20150072340A2 (en) 2015-03-12

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