HK1200912A1 - 精子染色和分選方法 - Google Patents
精子染色和分選方法Info
- Publication number
- HK1200912A1 HK1200912A1 HK15101291.7A HK15101291A HK1200912A1 HK 1200912 A1 HK1200912 A1 HK 1200912A1 HK 15101291 A HK15101291 A HK 15101291A HK 1200912 A1 HK1200912 A1 HK 1200912A1
- Authority
- HK
- Hong Kong
- Prior art keywords
- sorting methods
- sperm staining
- sperm
- staining
- sorting
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0608—Germ cells
- C12N5/061—Sperm cells, spermatogonia
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6432—Quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
- G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Zoology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Organic Chemistry (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Developmental Biology & Embryology (AREA)
- Reproductive Health (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201161541451P | 2011-09-30 | 2011-09-30 | |
US201161541438P | 2011-09-30 | 2011-09-30 | |
PCT/US2012/058008 WO2013049631A1 (en) | 2011-09-30 | 2012-09-28 | Sperm staining and sorting methods |
Publications (1)
Publication Number | Publication Date |
---|---|
HK1200912A1 true HK1200912A1 (zh) | 2015-08-14 |
Family
ID=47046868
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HK15101291.7A HK1200912A1 (zh) | 2011-09-30 | 2015-02-05 | 精子染色和分選方法 |
Country Status (5)
Country | Link |
---|---|
US (2) | US9581587B2 (zh) |
EP (1) | EP2761275B1 (zh) |
DK (1) | DK2761275T3 (zh) |
HK (1) | HK1200912A1 (zh) |
WO (1) | WO2013049631A1 (zh) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013049631A1 (en) | 2011-09-30 | 2013-04-04 | Inguran, Llc | Sperm staining and sorting methods |
US10620213B2 (en) | 2012-10-05 | 2020-04-14 | Inguran, Llc | High pressure sperm sorting and flow cytometer methods |
CN103525760B (zh) * | 2013-09-29 | 2016-09-21 | 大连金弘基种畜有限公司 | 性控精液的分离方法 |
WO2016081864A1 (en) * | 2014-11-20 | 2016-05-26 | Inguran, Llc | Low sugar sperm media and compositions |
WO2016118557A1 (en) * | 2015-01-20 | 2016-07-28 | President And Fellows Of Harvard College | Assessing metabolic condition of sperm |
EP3570853B1 (en) * | 2017-01-20 | 2024-07-24 | Inguran, LLC | Sperm processing method, apparatus and related media compositions |
WO2019199852A1 (en) | 2018-04-09 | 2019-10-17 | Inguran, Llc | Improved sperm nuclei and methods of their manufacture and use |
CN109738412B (zh) * | 2019-03-05 | 2021-07-13 | 贵州大学 | 一种饮用水中铝、铁、铬、汞、铕、铥和镱离子的检测方法 |
CN117965432B (zh) * | 2024-03-29 | 2024-06-14 | 内蒙古大学 | 一种通过nad+提高牛精子分选后活力的方法 |
Family Cites Families (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US139497A (en) | 1873-06-03 | Improvement in washing-machines | ||
US4559309A (en) | 1982-09-01 | 1985-12-17 | Memorial Sloan Kettering Cancer Center | Flow cytometry-fluorescence measurements for characterizing sperm |
JP2552582B2 (ja) | 1989-05-10 | 1996-11-13 | アメリカ合衆国 | 子の性の前選択の方法 |
US5895922A (en) | 1996-03-19 | 1999-04-20 | Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of National Defence | Fluorescent biological particle detection system |
US6149867A (en) | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
US7070917B1 (en) * | 1999-03-05 | 2006-07-04 | Preben Christensen | Determination of sperm concentration and viability for artificial insemination |
US7208265B1 (en) * | 1999-11-24 | 2007-04-24 | Xy, Inc. | Method of cryopreserving selected sperm cells |
US7019129B1 (en) | 2000-05-09 | 2006-03-28 | Biosearch Technologies, Inc. | Dark quenchers for donor-acceptor energy transfer |
AU1980202A (en) | 2000-11-22 | 2002-06-03 | Pharmacia Corporation | Methods and apparatus for producing gender enriched sperm |
US20060067916A1 (en) | 2002-07-22 | 2006-03-30 | Xy, Inc. | Sperm cell process system |
JP2006525019A (ja) | 2003-03-28 | 2006-11-09 | モンサント テクノロジー エルエルシー | 精子の染色方法 |
EP2305172B1 (en) | 2003-03-28 | 2016-05-11 | Inguran, LLC | Apparatus and methods for providing sex-sorted animal sperm |
CN101238369B (zh) | 2004-03-29 | 2012-07-04 | 英格朗公司 | 调节细胞内和/或细胞外氧化/还原反应的组合物在精子染色或分选方法中的用途 |
DE602005010515D1 (de) | 2004-08-06 | 2008-12-04 | Fuji Electric Holdings | Nachweisverfahren für lebenfähige Zellen |
BRPI0814343A2 (pt) * | 2007-07-23 | 2017-10-03 | Androgenix Ltd | Materiais e métodos para a seleção da sexagem do esperma |
GB0910826D0 (en) * | 2009-06-23 | 2009-08-05 | Ovasort Ltd | Identification of sex-linked proteins |
CA2778245A1 (en) * | 2009-10-30 | 2011-05-05 | Inguran, LLC. | Methods and systems for reducing dna fragmentation in a population of sperm cells |
CN102812123A (zh) | 2010-04-01 | 2012-12-05 | 英格朗公司 | 减少经处理的精子样品中的dna断裂的方法和系统 |
WO2013049631A1 (en) | 2011-09-30 | 2013-04-04 | Inguran, Llc | Sperm staining and sorting methods |
EP3492603B1 (en) | 2012-10-05 | 2022-07-20 | Inguran, LLC | Methods of processing sperm for sex sorting |
US10620213B2 (en) | 2012-10-05 | 2020-04-14 | Inguran, Llc | High pressure sperm sorting and flow cytometer methods |
-
2012
- 2012-09-28 WO PCT/US2012/058008 patent/WO2013049631A1/en active Application Filing
- 2012-09-28 EP EP12775394.5A patent/EP2761275B1/en active Active
- 2012-09-28 US US14/348,194 patent/US9581587B2/en active Active
- 2012-09-28 DK DK12775394.5T patent/DK2761275T3/en active
- 2012-09-28 US US13/631,249 patent/US9140688B2/en active Active
-
2015
- 2015-02-05 HK HK15101291.7A patent/HK1200912A1/zh unknown
Also Published As
Publication number | Publication date |
---|---|
US20130084558A1 (en) | 2013-04-04 |
US9581587B2 (en) | 2017-02-28 |
US20140234833A1 (en) | 2014-08-21 |
DK2761275T3 (en) | 2017-09-25 |
US9140688B2 (en) | 2015-09-22 |
EP2761275B1 (en) | 2017-06-21 |
WO2013049631A1 (en) | 2013-04-04 |
EP2761275A1 (en) | 2014-08-06 |
US20150072340A2 (en) | 2015-03-12 |
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