HK1060598A1 - Method of detecting nucleic acids using probes with fluorescent labels by analysis of the fluorescence melting curves - Google Patents

Method of detecting nucleic acids using probes with fluorescent labels by analysis of the fluorescence melting curves

Info

Publication number
HK1060598A1
HK1060598A1 HK04103632A HK04103632A HK1060598A1 HK 1060598 A1 HK1060598 A1 HK 1060598A1 HK 04103632 A HK04103632 A HK 04103632A HK 04103632 A HK04103632 A HK 04103632A HK 1060598 A1 HK1060598 A1 HK 1060598A1
Authority
HK
Hong Kong
Prior art keywords
probes
analysis
nucleic acids
fluorescent labels
detecting nucleic
Prior art date
Application number
HK04103632A
Other languages
English (en)
Inventor
Shun-Ichi Itoh
Toshinori Hayashi
Juichi Saitoh
Takahiko Ishiguro
Original Assignee
Tosoh Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tosoh Corp filed Critical Tosoh Corp
Publication of HK1060598A1 publication Critical patent/HK1060598A1/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
HK04103632A 2002-06-21 2004-05-21 Method of detecting nucleic acids using probes with fluorescent labels by analysis of the fluorescence melting curves HK1060598A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2002181573A JP2004024035A (ja) 2002-06-21 2002-06-21 核酸の検出方法

Publications (1)

Publication Number Publication Date
HK1060598A1 true HK1060598A1 (en) 2004-08-13

Family

ID=29717538

Family Applications (1)

Application Number Title Priority Date Filing Date
HK04103632A HK1060598A1 (en) 2002-06-21 2004-05-21 Method of detecting nucleic acids using probes with fluorescent labels by analysis of the fluorescence melting curves

Country Status (5)

Country Link
US (1) US20040014119A1 (xx)
EP (1) EP1375674B1 (xx)
JP (1) JP2004024035A (xx)
DE (1) DE60313828T2 (xx)
HK (1) HK1060598A1 (xx)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0303497D0 (en) * 2003-02-15 2003-03-19 Univ Liverpool Immuno PCR method
JP4501443B2 (ja) 2004-02-03 2010-07-14 東ソー株式会社 腸管出血性大腸菌の志賀毒素群遺伝子の検出試薬
CA2584569C (en) * 2004-10-18 2014-09-30 Brandeis University Primers, probes and methods for nucleic acid amplification
US20070065837A1 (en) * 2005-09-21 2007-03-22 Qiagen Diagnostics Gmbh Methods and compositions for the detection of Chlamydia trachomatis
EP1845166A1 (de) * 2006-04-13 2007-10-17 BioSpring GmbH Neue Nukleinsäuresonden mit kovalent gebundenen, interkalierenden Fluoreszenzfarbstoffen zur Hybridisierung an Nukleinsäureproben
JP4957069B2 (ja) * 2006-05-01 2012-06-20 ソニー株式会社 プローブ核酸配列設計方法、プローブ核酸、検出表面、プログラム、情報記憶媒体、並びにプローブ核酸設計システム
WO2008093002A2 (en) * 2007-02-01 2008-08-07 Abacus Diagnostica Oy Method for detection of presence of target polynucleotide in samples
US20100129796A1 (en) * 2008-11-24 2010-05-27 Micah Halpern Dye probe fluorescence resonance energy transfer genotyping
CA2770888C (en) 2009-08-31 2020-04-14 Gen-Probe Incorporated Dengue virus assay
WO2013034160A1 (en) * 2011-09-06 2013-03-14 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e. V. Methods for analyzing biological macromolecular complexes and use thereof
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
GB2506375B (en) * 2012-09-27 2017-10-18 Epistem Ltd Data processing and analysis systems
EP3130679B1 (de) 2015-08-13 2018-02-28 Cladiac GmbH Verfahren und testsystem zum nachweis und/oder quantifizieren einer ziel-nukleinsäure in einer probe
CN107573331B (zh) * 2017-04-25 2019-11-15 浙江大学 能够提高寡核苷酸链Tm值的标记化合物及其应用
WO2022092012A1 (ja) * 2020-10-30 2022-05-05 東洋紡株式会社 複数の標的核酸の同時検出方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2750504B1 (fr) * 1996-06-27 1998-08-28 Appligene Oncor Procede d'analyse d'acides nucleiques par hybridation et dispositif pour sa mise en oeuvre
JP3785517B2 (ja) * 1997-06-05 2006-06-14 東ソー株式会社 核酸融解温度測定法
US6140054A (en) * 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
DE10027113A1 (de) * 1999-12-23 2001-09-27 Andreas Hoeft Verfahren zur Bestimmung von mikrobieller DNS/RNS, Kit dafür und Verwendung des Verfahrens
US20040053230A1 (en) * 2001-03-07 2004-03-18 Schaffer Michael Edward Real time quantitative pcr with intercalating dye for single and multiplex target dna
CA2417986C (en) * 2000-08-11 2013-11-26 University Of Utah Research Foundation Single-labeled oligonucleotide probes
EP1275734A1 (en) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Method for random cDNA synthesis and amplification

Also Published As

Publication number Publication date
EP1375674B1 (en) 2007-05-16
EP1375674A1 (en) 2004-01-02
JP2004024035A (ja) 2004-01-29
US20040014119A1 (en) 2004-01-22
DE60313828D1 (de) 2007-06-28
DE60313828T2 (de) 2008-02-14

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