GB9926730D0 - Unactivated oocytes as cytoplast recipeints for nuclear transfer - Google Patents

Unactivated oocytes as cytoplast recipeints for nuclear transfer

Info

Publication number
GB9926730D0
GB9926730D0 GBGB9926730.4A GB9926730A GB9926730D0 GB 9926730 D0 GB9926730 D0 GB 9926730D0 GB 9926730 A GB9926730 A GB 9926730A GB 9926730 D0 GB9926730 D0 GB 9926730D0
Authority
GB
United Kingdom
Prior art keywords
time
embryo
transfer
recipeints
cytoplast
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GBGB9926730.4A
Other versions
GB2340493A (en
GB2340493B (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UK Government
Minister of Agriculture Fisheries and Food UK
Biotechnology and Biological Sciences Research Council
Roslin Institute Edinburgh
Original Assignee
UK Government
Minister of Agriculture Fisheries and Food UK
Biotechnology and Biological Sciences Research Council
Roslin Institute Edinburgh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9517779.6A external-priority patent/GB9517779D0/en
Application filed by UK Government, Minister of Agriculture Fisheries and Food UK, Biotechnology and Biological Sciences Research Council, Roslin Institute Edinburgh filed Critical UK Government
Priority to GB9926730A priority Critical patent/GB2340493B/en
Publication of GB9926730D0 publication Critical patent/GB9926730D0/en
Publication of GB2340493A publication Critical patent/GB2340493A/en
Application granted granted Critical
Publication of GB2340493B publication Critical patent/GB2340493B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Reproductive Health (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Plant Pathology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Epidemiology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Gynecology & Obstetrics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

A method of reconstituting an animal embryo involves transferring a diploid nucleus into an oocyte which is arrested in the metaphase of the second meiotic division. The oocyte is not activated at the time of transfer, so that the donor nucleus is kept exposed to the recipient cytoplasm for a period of time. The diploid nucleus can be donated by a cell in either the G0 or G1 phase of the cell cycle at the time of transfer. Subsequently, the reconstituted embryo is activated. Correct ploidy is maintained during activation, for example, by incubating the reconstituted embryo in the presence of a microtubule inhibitor much as nocodazole. The reconstituted embryo may then give rise to one or more live animal births. The invention is useful in the production of transgenic animals as well as non-transgenics of high genetic merit.
GB9926730A 1995-08-31 1996-08-30 Unactivated oocytes as cytoplast recipients for nuclear transfer Expired - Fee Related GB2340493B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB9926730A GB2340493B (en) 1995-08-31 1996-08-30 Unactivated oocytes as cytoplast recipients for nuclear transfer

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9517779.6A GB9517779D0 (en) 1995-08-31 1995-08-31 Biological manipulation
GB9926730A GB2340493B (en) 1995-08-31 1996-08-30 Unactivated oocytes as cytoplast recipients for nuclear transfer
GB9802916A GB2318792B (en) 1995-08-31 1996-08-30 Unactivated oocytes as cytoplast recipients for nuclear transfer

Publications (3)

Publication Number Publication Date
GB9926730D0 true GB9926730D0 (en) 2000-01-12
GB2340493A GB2340493A (en) 2000-02-23
GB2340493B GB2340493B (en) 2000-06-21

Family

ID=26307655

Family Applications (2)

Application Number Title Priority Date Filing Date
GB9926730A Expired - Fee Related GB2340493B (en) 1995-08-31 1996-08-30 Unactivated oocytes as cytoplast recipients for nuclear transfer
GB9802916A Expired - Fee Related GB2318792B (en) 1995-08-31 1996-08-30 Unactivated oocytes as cytoplast recipients for nuclear transfer

Family Applications After (1)

Application Number Title Priority Date Filing Date
GB9802916A Expired - Fee Related GB2318792B (en) 1995-08-31 1996-08-30 Unactivated oocytes as cytoplast recipients for nuclear transfer

Country Status (1)

Country Link
GB (2) GB2340493B (en)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2265909B (en) * 1992-04-01 1996-03-20 Inst Of Animal Physiology And Fusion of donor nucleus with recipient cytoplast in the absence of calcium ion
CA2145190A1 (en) * 1992-09-22 1994-03-31 The Government Of The United States Of America As Represented By The Sec Retary, Department Of Health And Human Services Use of taxol for treating lymphomas and breast cancer
GB9308271D0 (en) * 1993-04-21 1993-06-02 Univ Edinburgh Method of isolating and/or enriching and/or selectively propagating pluripotential animal cells and animals for use in said method
ATE255412T1 (en) * 1993-07-29 2003-12-15 Us Gov Health & Human Serv METHOD FOR TREATING ATHEROSCLERosis OR RESTENOSIS USING A MICROTUBULUS STABILIZER

Also Published As

Publication number Publication date
GB2340493A (en) 2000-02-23
GB2318792A (en) 1998-05-06
GB2318792B (en) 2000-06-21
GB2340493B (en) 2000-06-21
GB9802916D0 (en) 1998-04-08

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Legal Events

Date Code Title Description
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20130830