GB986385A - Anti-tumor agent hilamycin and process for the production thereof - Google Patents
Anti-tumor agent hilamycin and process for the production thereofInfo
- Publication number
- GB986385A GB986385A GB13826/61A GB1382661A GB986385A GB 986385 A GB986385 A GB 986385A GB 13826/61 A GB13826/61 A GB 13826/61A GB 1382661 A GB1382661 A GB 1382661A GB 986385 A GB986385 A GB 986385A
- Authority
- GB
- United Kingdom
- Prior art keywords
- hilamycin
- organic solvent
- alkaline
- antitumour agents
- hilamycins
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
- A61K31/7048—Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/06—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Animal Behavior & Ethology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
<PICT:0986385/C2/1> The antitumour agents and antibiotics Hilamycins A, B and C are obtained by cultivating a strain of Streptomyces rochei var. hilamyceticus in an aqueous nutrient medium under submerged aerobic conditions, preferably at 25-35 DEG C. Hilamycin A is a white solid or colourless syrup soluble in alcohols, acetone, esters, benzene and in water at alkaline pH, but insoluble in water at acid or neutral pH, petroleum ether and hexane; having a U.V. maximum at 235-240 mm ; having an I.R. spectrum as shown in Fig. 2; giving negative ferric chloride and Tollens reactions; having elemental analysis C=69.22, H=9.74 and (a )25 DEG D=0. Hilamycin B is a colourless syrup, having similar solubilities to Hilamycin A; a U.V. maximum at 200 mm ; I.R. bands at 3360, 3200, 2940, 2880, 1725, 1705, 1660, 1458, 1437, 1375, 1240, 1160, 1100, 1040, 964, 818, 745 and 695 cm.-1; elemental analysis C = 66.24, H = 8.05, O = 22.53 and N = 1.16 and (a )20 DEG D = 63 DEG (1% in methanol). Hilamycin C is a substance which is separated from Hilamycins A and B by its solubility in hexane or petroleum ether and has U.V. maxima at 226 and 258 mm , has I.R. bands at 2910, 2850, 1700, 1473, 1431, 1406, 1347, 1304, 1285, 1265, 1245, 1225, 1206, 1185, 1095, 932 and 712 cm.-1 and is active against bacteria. The antitumour agents Hilamycins A, B and C are purified by (1) extraction of the culture filtrate at an alkaline pH with an organic solvent and addition of a non-solvent thereto, (2) transferring them from aqueous solution to an organic solvent at acid or neutral pH, (3) transferring them from the organic solvent solution to an aqueous solution at weak alkaline pH, (4) precipitating them from aqueous solution by addition of acid or (5) adsorbtion of an alkaline extract of the culture filtrate on a phenol type ion exchange resin. The antitumour agents are separated from each other by (1) passing an organic solvent solution thereof over active carbon, eluting Hilamycin B and then A with methanol-acetone and Hilamycin C with methanol-benzene or (2) counter-current distribution. The antitumour agents are used to treat mouse leukaemia by intravenous injection.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2215260 | 1960-04-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB986385A true GB986385A (en) | 1965-03-17 |
Family
ID=12074864
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB13826/61A Expired GB986385A (en) | 1960-04-27 | 1961-04-17 | Anti-tumor agent hilamycin and process for the production thereof |
Country Status (3)
| Country | Link |
|---|---|
| DE (1) | DE1180089B (en) |
| FR (1) | FR1908M (en) |
| GB (1) | GB986385A (en) |
-
1961
- 1961-04-17 GB GB13826/61A patent/GB986385A/en not_active Expired
- 1961-04-27 FR FR860062A patent/FR1908M/en active Active
- 1961-04-27 DE DEZ8702A patent/DE1180089B/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| DE1180089B (en) | 1964-10-22 |
| FR1908M (en) | 1963-07-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| GB1226589A (en) | ||
| ES416045A1 (en) | Recovery of acids from aqueous solutions | |
| GB1395907A (en) | Antibiotics | |
| GB1083662A (en) | A novel antibiotic, methods for its production and therapeutic compositions containing it | |
| GB715362A (en) | Improvements in or relating to antimicrobial agent | |
| GB986385A (en) | Anti-tumor agent hilamycin and process for the production thereof | |
| GB1357538A (en) | New antibiotic | |
| GB1355734A (en) | Process for producing antibiotic material | |
| GB1163270A (en) | A new Antibiotic Enduracidin and a process for the production thereof | |
| GB1038242A (en) | Bleomycin and processes for preparation thereof | |
| GB1220770A (en) | Process for the purification of lactic acid | |
| GB1388878A (en) | Antibiotics process for their production and use in ruminant feed | |
| ES361544A1 (en) | Process for the production of a new antibiotic sf-733 substance | |
| SE328254B (en) | ||
| GB1057051A (en) | Tetrahexin, a new antibiotic | |
| Funaki et al. | Virocidin, a new antiviral and antibacterial antibiotic | |
| GB974562A (en) | The antibiotic a22765 and salts thereof | |
| GB1113501A (en) | Antibiotic ra-6950b and method of production | |
| US3023145A (en) | Protomycin and its production | |
| GB1053562A (en) | ||
| GB862685A (en) | New antibiotic designated pa 155a | |
| ES289379A1 (en) | Antibiotic danomycin and method of production | |
| ES467309A1 (en) | Thienamycin recovery | |
| GB1006547A (en) | The antibiotic, takamycin, and process for the production of the same | |
| GB993492A (en) | New antibiotic 9971r.p. and its process of preparation |