GB982241A - A process for the purification of interferon - Google Patents

A process for the purification of interferon

Info

Publication number
GB982241A
GB982241A GB2267660A GB2267660A GB982241A GB 982241 A GB982241 A GB 982241A GB 2267660 A GB2267660 A GB 2267660A GB 2267660 A GB2267660 A GB 2267660A GB 982241 A GB982241 A GB 982241A
Authority
GB
United Kingdom
Prior art keywords
chromatography
interferon
adjustment
deae cellulose
sulphomethylcellulose
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2267660A
Inventor
Derek Clissold Burke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Research Development Corp UK
Original Assignee
National Research Development Corp UK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Research Development Corp UK filed Critical National Research Development Corp UK
Priority to GB2267660A priority Critical patent/GB982241A/en
Publication of GB982241A publication Critical patent/GB982241A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Interferon is purified by concentrating an aqueous inorganic salt solution of interferon by pressure dialysis, subjecting the concentrate to chromatography on sulphomethylcellulose at pH 2 with an increasing chloride gradient and subjecting the purified material so obtained to one or more stages of chromatography on diethylaminoethylcellulose at pH of between 5 and 6 with an increasing phosphate gradient and recovering a fraction containing interferon of increased purity and concentration. The six stage purification of the example consists of (a) ammonium sulphate precipitation followed by pressure dialysis, (b) chromatography on sulphomethylcellulose at pH 2,0, (c) concentration and adjustment to pH 6,6, (d) chromatography on DEAE cellulose at pH 6,6, (e) adjustment to pH 4,5 and chromatography on DEAE cellulose at pH 4,5, (f) adjustment to pH 5,8 and chromatography on DEAE cellulose at pH 5,8. Step (d) may be carried out at pH 6,g to 8,0.
GB2267660A 1960-06-28 1960-06-28 A process for the purification of interferon Expired GB982241A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB2267660A GB982241A (en) 1960-06-28 1960-06-28 A process for the purification of interferon

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB2267660A GB982241A (en) 1960-06-28 1960-06-28 A process for the purification of interferon

Publications (1)

Publication Number Publication Date
GB982241A true GB982241A (en) 1965-02-03

Family

ID=10183307

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2267660A Expired GB982241A (en) 1960-06-28 1960-06-28 A process for the purification of interferon

Country Status (1)

Country Link
GB (1) GB982241A (en)

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