GB708749A - Process for the production of antibiotically active substances known as rhodomycins - Google Patents

Process for the production of antibiotically active substances known as rhodomycins

Info

Publication number
GB708749A
GB708749A GB20634/51A GB2063451A GB708749A GB 708749 A GB708749 A GB 708749A GB 20634/51 A GB20634/51 A GB 20634/51A GB 2063451 A GB2063451 A GB 2063451A GB 708749 A GB708749 A GB 708749A
Authority
GB
United Kingdom
Prior art keywords
acid
extracted
precipitated
butanol
rhodomycins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB20634/51A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer AG
Original Assignee
Bayer AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer AG filed Critical Bayer AG
Publication of GB708749A publication Critical patent/GB708749A/en
Expired legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/28—Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Virology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)

Abstract

An antibiotic rhodomycin is produced by growing Streptomyces purpurascens NRRL-2269 under aerobic conditions in a nutrient medium. Rhodomycin is amphoteric; empirical formula C20H29O7N; consists of two components having average molecular weight of 420; contains neither sulphur nor methoxy groups; yields red-coloured solutions in acidic media, purple-coloured solutions in neutral media and blue-coloured solutions in alkaline media; soluble at the isoelectric point (pH8.4-8.6) in alkanols having up to 4 carbon atoms, chloroform, ether, water and acetone; is precipitated from aqueous solutions by picric acid, picrolonic acid and perchloric acid; decomposes when heated with aqueous acidic media; forms salts with mineral salts and with alkalies; absorption band maxima in benzene at 575,542, 535, 504 millimicrons, in acetic anhydridepyroboric acetate mixture at 587, 542 and 505; effective against Staphylococcus aureus in dilutions of 1 in 30,000,000. Salts are also formed with stannous chloride, mercury chloride, chlorauric acid, potassium chromate, chloroplatinic acid, helianthin, alizarine sulphonic acid, anthraquinone sulphonic acid, iodine in potassium iodide, phosphotungstic acid, phosphomolybdic acid, b -naphthalene sulphonic acid, styphnic acid and strong mineral acids, e.g. hydrochloric, phosphoric and sulphuric. The nutrient medium contains a carbon source, e.g. glycerin, glucose or dextrin, a nitrogen source, e.g. glycocoll, alkali metal nitrates, glycine ethyl ester hydrochloride or tyrosine, and mineral sources, e.g. sodium chloride, secondary potassium phosphate, ammonium sulphate, ferrous sulphate, calcium carbonate and Hoagland's solution. Yeast liquor or corn steep liquor may be present. Rhodomycins are obtained from the culture solution or mycelium. The culture solution is concentrated, acetone and basic lead acetate added, and extracted with butanol at pH 8.6. Butanol solution is extracted with 10 per cent acetic acid and the rhodomycins precipitated as picrate. The powdered mycelium is extracted with acidified acetone water or acidified methanol-water, the mycelium centrifuged off, and impurities precipitated from the neutralized centrifugate from which the organic solvent has been removed, by basic lead acetate. The aqueous filtrate is extracted with butanol, and then the butanol extract is extracted with aqueous acetic acid. The antibiotic is precipitated from the acid extract by picric acid.
GB20634/51A 1950-09-02 1951-08-31 Process for the production of antibiotically active substances known as rhodomycins Expired GB708749A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE708749X 1950-09-02

Publications (1)

Publication Number Publication Date
GB708749A true GB708749A (en) 1954-05-12

Family

ID=6617679

Family Applications (1)

Application Number Title Priority Date Filing Date
GB20634/51A Expired GB708749A (en) 1950-09-02 1951-08-31 Process for the production of antibiotically active substances known as rhodomycins

Country Status (1)

Country Link
GB (1) GB708749A (en)

Similar Documents

Publication Publication Date Title
Suzuki et al. A new antibiotic, polyoxin A
GB715362A (en) Improvements in or relating to antimicrobial agent
GB1339032A (en) Rabelomycin and processes for its production
GB708749A (en) Process for the production of antibiotically active substances known as rhodomycins
US3022286A (en) Process for preparing higher fatty acid salts of neomycin
GB1063255A (en) The antibiotic anguidine and derivatives thereof
GB977327A (en) An antibiotic moenomycin and a process for its manufacture
Matsuoka et al. Unamycin, an antifungal substance produced by Streptomyces fungicidicus
GB1292010A (en) A new antibiotic, its production and its use
Suzuki et al. Neopterin-3'-β-D-glucuronide: Isolation from Azotobacter agilis
GB1178783A (en) Antibiotic K-16
GB729208A (en) Improvements in or relating to a new antibiotic p.a. 95 and the preparation thereof
GB1388878A (en) Antibiotics process for their production and use in ruminant feed
GB1057424A (en) New antibiotic and process for its manufacture
SE7708939L (en) ANTIBIOTICS
SU1475150A1 (en) Antibiotic eremomycin and method of its preparing
GB829327A (en) Novel antibiotic designated marcomycin and process of making same
GB1356723A (en) Antibiotic sf-1293 substance and its production and uses
GB912339A (en) Process for the manufacture of the antibiotic pilosomycin, its components and salts thereof
GB850325A (en) Production of a new antibiotic flavensomycin
GB684417A (en) A new antibiotic and a process for the production thereof
GB783486A (en) A new antibiotic filipin
GB762736A (en) The new antibiotic b-mycin and processes for its preparation
GB1107112A (en) Antibiotic josamycin and processes for the production thereof
GB817385A (en) Process for the production of an antibiotic designated kanamycin