GB658669A - Improvements in or relating to the preparation of one or more basic amino acids from a mixture of amino acids - Google Patents
Improvements in or relating to the preparation of one or more basic amino acids from a mixture of amino acidsInfo
- Publication number
- GB658669A GB658669A GB3416/49A GB341649A GB658669A GB 658669 A GB658669 A GB 658669A GB 3416/49 A GB3416/49 A GB 3416/49A GB 341649 A GB341649 A GB 341649A GB 658669 A GB658669 A GB 658669A
- Authority
- GB
- United Kingdom
- Prior art keywords
- acid
- resin
- adsorbed
- amino acids
- buffer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C227/38—Separation; Purification; Stabilisation; Use of additives
- C07C227/40—Separation; Purification
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
One or more basic amino acids are separated from a mixture of amino acids by passing a solution of the mixture through a bed or column of "carboxylic type" ion-exchange resin containing carboxylic acid groups and carboxylic salt groups in a ratio that has been adjusted so that the pH of the amino acid solution in contact with the resin is maintained below the isoelectric point of the acid or acids to be adsorbed and above that of the acid or acids not to be adsorbed, and thereafter eluting adsorbed acid from said resin. The "carboxylic type" resins are those wherein the cation-adsorbing groups are carboxyl groups, and are made by copolymerizing styrene, maleic anhydride, and a compound containing non-conjugated double bonds, one in a CH2=C< grouping, or by copolymerizing an acrylic acid CH2= CR-COOH with a compound having two non-conjugated double bonds, as described in U.S.A. Specifications 2,340,110 and 2,340,111. Adjustment of the ratio of acid to salt groups in the resin is effected by converting it to its sodium, ammonium or other alkali metal salt by treating with alkali hydroxide solution, and adding a buffer solution such as sodium acetate-acetic acid or containing other salts of strong bases and weak acids, e.g. alkali metal salts of phosphoric, citric, propionic or phthalic acid. A resin which operates at pH 6.3-7.6 to adsorb basic amino acids but not neutral or acidic is prepared by treating the sodium salt of the resin with a buffer of pH 4.2-5.5. A resin may be prepared to operate at pH 7.6-9.7 to adsorp arginine and lysine but not histidine by using a buffer of pH 5.8-7.5. By a sequence of operations, separation in steps may be effected. Two beds of resin may sometimes be used in each step. The resin may be treated with 0.1 N sodium hydroxide to form the salt, rinsed with water until the effluent has a pH of 10, treated with buffer solution of the proper pH, and again rinsed. The solution of amino acids should have a pH approximately that to prevail in the column. A flow rate of 1 volume of 0.1 per cent amino acid solution per 10 volumes of resin per minute is suitable. Removal of adsorbed acid may be effected with strong or weak acids, e.g. 0.01 N mineral acid or aqueous solutions of carbon dioxide under pressure, or with alkali such as sodium hydroxide or ammonia solution. Protein hydrolysates made with strong mineral acids are treated by steam distillation or by addition of an acid-adsorbing resin such as is described in Specification 556,622 to raise the pH to about 3 without amino acids being adsorbed. The preferred carboxylic type resins are derived from acrylic, methacrylic or maleic acid and an aromatic divinyl compound such as divinyl benzene or naphthalene. The latter is generally present in amount of less than 10, preferably 5, per cent. In examples: (1) a mixture containing arginine, lysine, histidine, leucine, and glutamic acid is passed through divinyl benzene-methacrylic acid resin which has been treated with sodium hydroxide and a buffer of pH 4.7, arginine, lysine, and histidine being adsorbed; (2) arginine and lysine are separated from histidine using a buffer of pH 7.0; (3) a mixture of amino acids as in (2) but of pH about 10 is treated with the resin converted to sodium salt, only arginine being adsorbed, the effluent being neutralized and treated with resin to which a buffer of pH is added, the remaining arginine and lysine being adsorbed.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US286274XA | 1948-03-16 | 1948-03-16 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB658669A true GB658669A (en) | 1951-10-10 |
Family
ID=21844516
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB3416/49A Expired GB658669A (en) | 1948-03-16 | 1949-02-08 | Improvements in or relating to the preparation of one or more basic amino acids from a mixture of amino acids |
Country Status (3)
Country | Link |
---|---|
CH (1) | CH286274A (en) |
GB (1) | GB658669A (en) |
NL (2) | NL145241B (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2949450A (en) * | 1955-07-18 | 1960-08-16 | John B Stark | Separations of nitrogenous organic compounds |
CN103145573A (en) * | 2013-03-27 | 2013-06-12 | 中粮生物化学(安徽)股份有限公司 | Purification method of lysine |
-
0
- NL NL70222D patent/NL70222C/xx active
- NL NL707003014A patent/NL145241B/en unknown
-
1949
- 1949-02-08 GB GB3416/49A patent/GB658669A/en not_active Expired
- 1949-03-09 CH CH286274D patent/CH286274A/en unknown
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2949450A (en) * | 1955-07-18 | 1960-08-16 | John B Stark | Separations of nitrogenous organic compounds |
CN103145573A (en) * | 2013-03-27 | 2013-06-12 | 中粮生物化学(安徽)股份有限公司 | Purification method of lysine |
Also Published As
Publication number | Publication date |
---|---|
NL70222C (en) | |
NL145241B (en) | |
CH286274A (en) | 1952-10-15 |
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