GB486366A - Improvements in and relating to processes for the production of nucleosides - Google Patents
Improvements in and relating to processes for the production of nucleosidesInfo
- Publication number
- GB486366A GB486366A GB1900437A GB1900437A GB486366A GB 486366 A GB486366 A GB 486366A GB 1900437 A GB1900437 A GB 1900437A GB 1900437 A GB1900437 A GB 1900437A GB 486366 A GB486366 A GB 486366A
- Authority
- GB
- United Kingdom
- Prior art keywords
- solution
- acid
- water
- adenosine
- picrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Saccharide Compounds (AREA)
Abstract
Guanosine and adenosine are produced by causing emulsion to act on nucleic acids at a pH value of 4-5,5 and preferably at a slightly elevated temperature, e.g. 30-40 DEG C., crystallizing and separating the guanosine formed, precipitating the adenosine in the form of its picrate, and decomposing the picrate with a basic substance, preferably one forming a difficultly soluble picrate, e.g. potash or ammonia. The action of the emulsion should be continued preferably until the splitting off of phosphoric acid ceases, this occurring after about 8-14 or more days. The nucleic acids may be preliminarily degraded before treatment with emulsin by the action of alkali, e.g. a liquor having a 2-5 per cent alkali concentration. After heating on a water bath for a few hours, the hydrogen ion concentration is brought to the necessary value, e.g. by the addition of acetic acid, before the addition of emulsin. When an aqueous solution of picric acid is p used, it is generally necessary to evaporate the mixture in vacuum before the addition of the picric acid. The phosphoric acid can be removed by treatment with baryta water and excess barium removed by sulphuric acid. Evaporation can be entirely or in part dispensed with if solid picric acid is added to the solution freed from guanosine and albumen, but which may contain some sulphuric acid. In an example, a mixture of yeast nucleic acid and caustic solution is heated on a water bath for two hours. When the solution has cooled to 40 DEG C., acetic acid solution at 40 DEG C. and then an emulsion solution and a few drops of toluene are added. The solution is placed in an incubator at 37 DEG C. and is shaken daily. After 12-14 days, the solution is placed in an ice chest, and the guanosine formed is filtered and washed with water, alcohol and ether. The filtrate is boiled and cooled, and albumen then filtered off. Phosphoric acid is removed as above. The solution is evaporated somewhat, and heated to 50 DEG C., dry picric acid being then stirred in. After standing in an ice chest, adenosine picrate is filtered off, and washed with water, alcohol and ether. The picrate is crushed, treated in an Erlenmerger flask with water, and heated to 55 DEG C. Concentrated potash solution is then added, and after standing in ice water, potassium picrate is filtered off. The filtrate is seeded with adenosine, and after standing in an ice chest, the paste is filtered off, washed with water and then with acetone. The adenosine may be recrystallized from water. A 25 per cent ammonia solution may be used in place of the potash solution. Cytidine and uridine can be obtained from the filtrate obtained after precipitation of the adenosine with picric acid. A statement of the prior art refers to the action on yeast nucleic acid of juices expressed from spinach, or Cortinellus edodes or nucleotidases from liver, or intestinal mucus. The adenosine is isolated in the form of its picrate, which is decomposed by sulphuric acid. The yield is very small.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB1900437A GB486366A (en) | 1937-07-08 | 1937-07-08 | Improvements in and relating to processes for the production of nucleosides |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB1900437A GB486366A (en) | 1937-07-08 | 1937-07-08 | Improvements in and relating to processes for the production of nucleosides |
Publications (1)
Publication Number | Publication Date |
---|---|
GB486366A true GB486366A (en) | 1938-06-02 |
Family
ID=10122130
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB1900437A Expired GB486366A (en) | 1937-07-08 | 1937-07-08 | Improvements in and relating to processes for the production of nucleosides |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB486366A (en) |
-
1937
- 1937-07-08 GB GB1900437A patent/GB486366A/en not_active Expired
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