GB474822A - Process for the production of specifically active enzyme preparations - Google Patents

Process for the production of specifically active enzyme preparations

Info

Publication number
GB474822A
GB474822A GB3130/36A GB313036A GB474822A GB 474822 A GB474822 A GB 474822A GB 3130/36 A GB3130/36 A GB 3130/36A GB 313036 A GB313036 A GB 313036A GB 474822 A GB474822 A GB 474822A
Authority
GB
United Kingdom
Prior art keywords
preparations
enzymes
dried
parts
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB3130/36A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of GB474822A publication Critical patent/GB474822A/en
Expired legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/36Adaptation or attenuation of cells
    • AHUMAN NECESSITIES
    • A21BAKING; EDIBLE DOUGHS
    • A21DTREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D6/00Other treatment of flour or dough before baking, e.g. cooling, irradiating, heating

Abstract

Enzyme preparations are obtained by treating micro-organisms containing several enzyme complexes, for example by drying, heating in media rich in albumen, so as to kill the cells while not destroying all the enzymic activity, and liberating "endo-enzymes," subjecting to low temperature, or treating with chemicals such as acetone or glycerin to kill the cells and remove certain enzymes such as lipase, while maintaining a residual activity of other enzymes such as proteinases and zymases. The products may be mized with one another or with other enzyme materials (such as diastase, proteinase, or grain germs) and with substrates (such as carbohydrates and albumens, particularly milk and dried milk preparations) and activators such as organic acids (lactic acid and vinegar) materials containing co-zymases and organic substances containing phosphorus such as nucleo-proteids. The preparations may be used as baker's assistants for the production of bread, the production of malt, cheese, the rotting of manure, the retting of fibres, the fermentation of tobacco, cacao and coffee and other processes for the swelling and loosening of organic materials. For the production of malt, the preparation should contain diastatic, proteolytic and zymatic properties, the cellulose and pectinase complexes being destroyed by means of bacteria or mould fungi which destroy cellulose and contain pectin. Such preparations are suitable for rotting manure and retting fibres. For the fermentation of tobacco the proteolytic enzyme is "selectively fortified." For cheese-making the proteolytic enzyme is "selectively fortified" and enzymes containing lactase which may be obtained from milk-sugar yeast, may be added, such yeasts being living or partly destroyed while maintaining the lactase activity. For the fermentation of cacao and coffee preparations are used in which the pectinases and germination ferment complexes are "selectively fortified" relatively to the other ferment complexes present. As starting materials may be used bacteria rich in pectinase of the coli-aerogenes group and moulds rich in pectinase such as aspergillus; such preparations may be used together with material containing zymase and proteinase for instance from schizo-saccharomyces. To the preparations may be added various specified materials for promoting or inhibiting the action of particular enzymes: tryptic enzymes may be inhibited by organic acids of pH at most 5; and for promoting zymase action magnesium sulphate sufficient to give a magnesium concentration of 0,01 to 0,03 per cent in the medium in which the preparation is to be used. In examples: (1) a baker's assistant consists of 30 parts schizo-saccharomyces dried at 60--65 DEG C. for at least a minute, preferably under reduced pressure, 10 to 15 parts of an aspergillus rich in diastase dried in a similar manner, 30 parts bran flour, 5 parts wheat germ, and 25 to 20 parts of dried milk. The schizo-saccharomyces bomb may be cultivated in known manner in a dilute beer wort free from hops, 5 per cent raw sugar with additional nutrients, or grape must culture medium. The cultivation may be at 30 DEG C. with aeration, and the organism may be separated from the medium, washed and dried as such or after dilution. For the drying the pulp is supplied in a thin layer to a heated rotary drum with a stripper blade at one side, the vapour p being withdrawn by a vacuum pump. Instead of drying the pure yeast, the culture medium containing it may be dried. The drying kills the cells, and inactivates the zymase and enzymes which break down low-molecular albuminous materials while proteinase is less affected. In the cultivation of aspergillus it is important that no spores should be formed, as they are resistant to heat; (2) a baker's assistant consists of 40 parts of an autolyzed aspergillus of the torula, mycotorula, anomalus, mycoderma oidium and monilia groups, preferably those which are not rich in lipase; the organisms are cultivated in known manner, separated from the medium, killed by a suitable antiseptic such as chloroform or toluene and autolyzed at 40 DEG C. and dried rapidly under reduced pressure; 15 parts of dried aspergillus, 10 parts wheat germ, 35 parts "excess from the preparation of the grain flour"; (3) for the fermentation of cacao the preparation contains 60 parts of aspergillus dried at 50--60 DEG C. while killing all the cells, and 40 parts of a preparation obtained by drying in the manner described above pectinase-containing bacteria of the coli-aerogenes group in which the cells are destroyed. Preferably the preparation is used together with a pure yeast such as dako-schizo-saccharomyces.ALSO:Enzyme preparations are obtained by treating micro-organisms containing several enzyme complexes, for example by drying, heating in media rich in albumen so as to kill the cells while not destroying all the enzyme activity, and liberating "endo-enzymes," subjecting to low temperature, or treating with chemicals such as acetone or glycerin to kill the cells and remove certain enzymes such as lipase, while maintaining a residual activity of other enzymes such as proteinases and zymases. The products may be mixed with one another or with other enzyme materials (such as diastase, proteinase, or grain germs) and with substrates (such, as carbohydrates and albumens, particularly milk, and dried milk preparations) and, activators such as organic acids (lactic acid and vinegar) materials containing co-zymases and organic substances containing phosphorus such as nucleo-proteids. The preparations may be used for the production of cheese, and the rotting of manure and other processes for the swelling and loosening of organic materials. For the production of malt, the preparation should contain diastatic, proteolytic and zymatic properties, the cellulose and pectinase complexes being destroyed by means of bacteria or mould fungi which destroy cellulase and contain pectin. Such preparations are suitable for rotting manure and retting fibres. For cheese-making the proteolytic enzyme is "selectively fortified" and enzymes containing lactase which may be obtained from milk-sugar yeast, may be added, such yeast being living or partly destroyed while maintaining the lactase activity. To the preparations may be added various specified materials for promoting or inhibiting the action of particular enzymes; tryptic enzymes may be inhibited by organic acids of pH at most 5; and for promoting zymase-action magnesium sulphate sufficient to give a magnesium concentration of 0,01 to 0,03 per cent in the medium in which the preparation is to be used.ALSO:Enzyme preparations are obtained by treating micro-organisms containing several enzyme complexes, for example by drying, heating in media rich in albumen, so as to kill the cells while not destroying all the enzyme activity, and liberating "endo-enzymes," subjecting to low temperature, or treating with chemicals such as acetone or glycerin to kill the cells and remove certain enzymes such as lipase, while maintaining a residual activity of other enzymes such as proteinases and zymases. The products may be mixed with one another or with other enzyme materials (such as diastase, proteinase, or grain germs) and with substrates (such as carbohydrate and albumens, particularly milk, and dried milk preparations) and activators such as organic acids (lactic acid and vinegar) materials containing cozymases and organic substances containing phosphorus such as nucleo-proteids. The preparations may be used as bakers' assistants for the production of bread, the production of malt, the fermentation of tobacco, cacao and coffee and other processes for the swelling and loosening of organic materials. For the production of malt, the preparation should contain diastatic, proteolytic and zymatic properties, the cellulose and pectinase, complexes being destroyed by means of bacteria or mould fungi which destroy cellulase and contain pectin. For the fermentation of tobacco the proteolytic enzyme is "selectively fortified." For the fermentation of cacao and coffee preparations are used in which the pectinases and germination ferment complexes are "selectively fortified" relatively to the other ferment complexes present. As starting materials may be used bacteria rich in pectinase of the coli-aerogenes group and moulds rich in pectinase such as aspergillus; such preparations may be used together with material containing zymase and proteinase, for instance from schizo-saccharomyces. To the preparations may be added various specified materials for promoting or inhibiting the action of particular enzymes; tryptic enzymes may be inhibited by organic acids of pH at most 5; and for promoting zymase-action magnesium sulphate sufficient to give a magnesium concentration of 0,01 to 0,03 per cent in the medium in which the preparation is to be used. In examples: (1) a baker's assistant consists of 30 parts schizo-saccharomyces dried at 60-65 DEG C. for at least a minute, preferably under reduced pressure, 10 to 15 parts of an aspergillus rich in diastase dried in a similar manner, 30 parts bran flour, 5 parts wheat germs, and 25 to 20 parts of dried milk. The schizo-saccharomyces bomb may be cultivated in known manner in a dilute beer wort free from hops, 5 per cent raw sugar with additional nutrients, or grape must culture medium. The cultivation may be at 30 DEG C. with aeration, and the organism may be separated from the medium, washed and dried as such or after dilution. For the drying the pulp is supplied in a thin layer to a heated rotary drum with a stripper blade at one side, the vapour being withdrawn by a vacuum pump. Instead of drying the pure yeast, the culture medium containing it may be dried. The drying kills the cells, and inactivates the zymase and enzymes which break down low-molecular albuminous
GB3130/36A 1935-02-01 1936-02-01 Process for the production of specifically active enzyme preparations Expired GB474822A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE474822X 1935-02-01

Publications (1)

Publication Number Publication Date
GB474822A true GB474822A (en) 1937-11-08

Family

ID=6541771

Family Applications (1)

Application Number Title Priority Date Filing Date
GB3130/36A Expired GB474822A (en) 1935-02-01 1936-02-01 Process for the production of specifically active enzyme preparations

Country Status (1)

Country Link
GB (1) GB474822A (en)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2665215A (en) * 1949-09-30 1954-01-05 Wallerstein Co Inc Method of making bakery products and composition therefor
US2693440A (en) * 1952-11-25 1954-11-02 Nat Dairy Res Lab Inc Drying of yeast to inactivate zymase and preserve lactase
US2715601A (en) * 1953-06-17 1955-08-16 Nat Dairy Res Lab Inc Lactase enzyme preparation
US2762749A (en) * 1951-05-02 1956-09-11 Nat Dairy Res Lab Inc Production of lactase
US2762748A (en) * 1953-03-31 1956-09-11 Nat Dairy Res Lab Inc Drying of yeast to inactivate zymase and preserve lactase
US2776928A (en) * 1955-05-23 1957-01-08 Nat Dairy Res Lab Inc Production of saccharomyces fragilis with an optimum yield of lactase
EP0036737A3 (en) * 1980-03-24 1983-05-11 Pfizer Inc. Lactase preparation
CN112456653A (en) * 2020-10-27 2021-03-09 广西诺亚生物科技发展有限公司 Compound for adjusting aquaculture water quality and preparation process thereof
CN117243885A (en) * 2023-11-15 2023-12-19 北京青藤谷禧干细胞科技研究院有限公司 Stem cell exosome composition for improving skin and preparation method thereof

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2665215A (en) * 1949-09-30 1954-01-05 Wallerstein Co Inc Method of making bakery products and composition therefor
US2762749A (en) * 1951-05-02 1956-09-11 Nat Dairy Res Lab Inc Production of lactase
US2693440A (en) * 1952-11-25 1954-11-02 Nat Dairy Res Lab Inc Drying of yeast to inactivate zymase and preserve lactase
US2762748A (en) * 1953-03-31 1956-09-11 Nat Dairy Res Lab Inc Drying of yeast to inactivate zymase and preserve lactase
US2715601A (en) * 1953-06-17 1955-08-16 Nat Dairy Res Lab Inc Lactase enzyme preparation
US2776928A (en) * 1955-05-23 1957-01-08 Nat Dairy Res Lab Inc Production of saccharomyces fragilis with an optimum yield of lactase
EP0036737A3 (en) * 1980-03-24 1983-05-11 Pfizer Inc. Lactase preparation
CN112456653A (en) * 2020-10-27 2021-03-09 广西诺亚生物科技发展有限公司 Compound for adjusting aquaculture water quality and preparation process thereof
CN117243885A (en) * 2023-11-15 2023-12-19 北京青藤谷禧干细胞科技研究院有限公司 Stem cell exosome composition for improving skin and preparation method thereof
CN117243885B (en) * 2023-11-15 2024-01-26 北京青藤谷禧干细胞科技研究院有限公司 Stem cell exosome composition for improving skin and preparation method thereof

Similar Documents

Publication Publication Date Title
Underkofler et al. Production of microbial enzymes and their applications
US4216235A (en) Elimination of flatulent sugars from soy
US20090004331A1 (en) Process of Producing Rooibos Tea Extract
KR20080046225A (en) Method of producing fungal culture
GB474822A (en) Process for the production of specifically active enzyme preparations
KR101983039B1 (en) Method for Manufacturing Nuruk Sauce
Beckhorn et al. Food additives, production and use of microbial enzymes for food processing
Smythe Microbiological production of enzymes and their industrial applications
KR20100136813A (en) Rice nuruk containing lactic acid bacteria and method of manufacturing rice makkoli using the above
CN107183235B (en) Method for preparing novel Pu' er tea
US1391219A (en) Enzymic substance and process of making the same
CN111034923A (en) Preparation process of fermented beef
CN109182204A (en) A kind of microbe leaven and preparation method thereof
US1460736A (en) Enzymic substance and process of making the same
US2077447A (en) Process of chillproofing and stabilizing beers and ales
US2077449A (en) Process of chillproofing and stabilizing malt beverages
US6090603A (en) Method for producing enzymes and, respectively, enzyme complexes
Kadiri et al. Application of Microbial Enzyme in Food Biotechnology
CN117801967B (en) Method for culturing Eurotium cristatum and application
KR102522128B1 (en) Method for manufacturing Takju with improved palatability and stability
KR890005275B1 (en) Process for eliminating ill soyben flour from tempeh
SU68061A1 (en) The method of fermentation of soybeans and similar protein-containing raw materials
CN105695224A (en) Shiitake mushroom and black garlic wine
Potter et al. Fermentations and Other Uses of Microorganisms
CN117837702A (en) Processing method of purple potato enzyme beverage