GB2594955A

GB2594955A - Seedstocks of Sphagnum

Info

Publication number: GB2594955A
Application number: GB2006992.8A
Authority: GB
Inventors: Wright Neal
Original assignee: Micropropagation Services EM Ltd
Current assignee: Micropropagation Services EM Ltd
Priority date: 2020-05-12
Filing date: 2020-05-12
Publication date: 2021-11-17
Anticipated expiration: 2040-05-12
Also published as: GB2594955B; GB202006992D0

Abstract

A method of providing a seedstock of Sphagnum is provided. The method comprises providing in vitro Sphagnum and applying the Sphagnum to a growth surface, cultivating the Sphagnum in vivo on the growth surface, harvesting the cultivated Sphagnum from the growth surface and chopping the harvested Sphagnum to provide a seedstock of Sphagnum for cultivation, the seedstock comprising a plurality of fragments of the in vivo Sphagnum. The fragments may have a length of between 5 and 50 mm. The growth surface may comprise compost, soil, peat and/or dried Sphagnum. The chopping of the harvested Sphagnum may comprise chopping such that at least 50% by weight of the fragments of in vivo Sphagnum have a length of at least 5mm. The method may further comprise chopping Sphagnum harvested from the seedstock to provide a second seedstock of Sphagnum for cultivation, the second seed stock comprising a plurality of fragments of second-generation in vivo Sphagnum. Sphagnum obtainable by the method is also disclosed.

Description

SEEDSTOCKS OF SPHAGNUM

The present disclosure relates to Sphagnum, in particular to methods of providing a seedstock of Sphagnum.

Sphagnum is a genus of moss. It is a lower plant, or a non-vascular plant, and is an example of a bryophyte. It is often referred to as peat moss and typically grows in the wild in peatlands or wetlands. Examples of suitable habitats for Sphagnum include bogs, such as raised bogs and blanket bogs, moors, mires, and fens. Sphagnum has a particularly high capacity for maintaining water in its hyaline cells. As such, in its natural environment, Sphagnum typically grows in wet conditions such as in peatlands.

Peatlands around the world are formed when lower layers of Sphagnum decay to form peat, while the upper layer continues to grow on the surface. As a result of this, carbon is stored within the peat while the actively-growing upper Sphagnum continues sequestering carbon dioxide from the atmosphere. Peatlands cover approximately 3% of the land on the Earth's surface, but store over 500 Gigatonnes of carbon -more than all other vegetation types combined. However, due to adverse impacts on the peatlands (e.g. industrial pollution, drainage -particularly for agriculture, and peat harvesting) the actively-growing upper Sphagnum has been eroded (or is now absent) in many peatlands, thereby exposing the peat to the atmosphere. This absence of surface Sphagnum enables carbon to be released from the peatland. This is a pressing environmental issue, and damaged peatlands now contribute around 6% of global anthropogenic carbon dioxide emissions. As a result, there is a pressing need for effective peatland restoration and methods of effectively growing Sphagnum for restoration purposes. Conventional methods of peatland restoration typically involve translocating Sphagnum from other sites including peatlands, which is clearly not sustainable.

Peat is also used as horticultural growing media. As this peat is harvested from the wild, this damages peatlands and ultimately exacerbates carbon emissions. There is a growing demand for alternatives to peat in growing media, and Sphagnum itself has been identified as a key peat alternative. Therefore, there exists a need for an effective method of growing Sphagnum for the purposes of harvest, such as for growing media.

Unlike higher plants or vascular plants, Sphagnum does not have roots. This has several implications, one of which is that water is not primarily taken up from below (e.g. from roots), and instead water is absorbed through the main body of the plant, such as the stem, leaves, or branches. Sphagnum spreads primarily from new offshoots called innovations. As used herein, the term "innovation" may otherwise be referred to as a "growing point". An individual plant of Sphagnum grows in a strand. A strand of Sphagnum comprises a stem with small leaves arranged intermittently along the length of the stem. The strand also comprises larger branches shooting off from the stem. At the top of the stem, the strand comprises a capitulum which is a head of new innovations. The capitulum is the primary growth point of Sphagnum. Due to this, water is primarily desired at the top of Sphagnum, which makes good use of collecting water e.g. from rain or irrigation from above. Sphagnum can also comprise innovations where branches sprout off from the main stem. The innovations can eventually result in new stems which break off from the original stem to form a new strand of Sphagnum. Multiple strands of Sphagnum together grow as a hummock. The creation of new strands is a result of vegetative reproduction of Sphagnum, and allows Sphagnum to grow into carpets covering a surface. Sphagnum can also reproduce via spores, but the abundance of spores of Sphagnum is often very low in the wild, and at most sites reproduction via spores is limited, and in any case is limited to a specific and narrow window of time in the year. As such, vegetative reproduction often dominates.

Another property of Sphagnum resulting from its lack of roots is that it cannot be planted in conventional ways. Typical methods of applying Sphagnum to a surface involve partially burying a clump of Sphagnum, such as in undergrowth on a peatland. Providing clumps of Sphagnum results in a non-uniform coverage of Sphagnum in the discrete areas of the clumps, and requires a large amount of starting material which can be expensive, especially if a high degree of initial coverage is desired. As Sphagnum is often translocated from other wild sources, using a large amount of starting material is of course damaging to the environment. Where a sustainable production of Sphagnum is possible, for example through tissue culture, the damage to the environment can be mitigated, but the cost of providing an initial large amount of starting material can be expensive, and often prohibitively so.

Other methods of applying Sphagnum include dispersing strands of Sphagnum onto a surface, such as an exposed peatland for restoration. Such methods are ineffective as the strands are not secured to the ground, often dry out before establishing and anchoring themselves, and blow away in wind or rain -both of which are particularly significant factors on peatlands.

The present disclosure seeks to address one or more of the above problems.

Aspects of the invention are set out in the independent claims and preferred features are set out in the dependent claims.

According to a first aspect of the present disclosure, there is provided a suspension of Sphagnum, comprising: a fluid solution comprising: water; and a thickening agent dissolved in the water, wherein the thickening agent comprises a cellulose-based or a starch-based thickening agent; and a plurality of fragments of Sphagnum suspended in the solution.

Disclosed herein is a suspension of Sphagnum, comprising: a fluid solution comprising: water; a thickening agent; and a plurality of fragments of Sphagnum suspended in the solution. Optionally, the suspension further comprises nutrients. Optionally, the nutrients comprise calcium. Optionally, the thickening agent comprises a cellulose-based thickening agent. Optionally, the thickening agent comprises a starch-based thickening agent.

The inventors have developed the suspension of the present disclosure which provides a medium for carrying Sphagnum to allow effective spreading of Sphagnum onto a surface, for example onto a peatland for restoration or onto a surface for propagation, e.g. in a greenhouse or on a field. Optionally, the suspension is suitable for coating a surface, in particular a growth surface on which Sphagnum can be grown. Optionally, the Sphagnum in the suspension is suitable for growth. In other words, it is not dried out or otherwise dead.

Preferably, the suspension is not toxic to the Sphagnum.

The suspension comprises a fluid solution. The fluid solution comprises water. Preferably, the water may be rainwater, or other low-salt water such as distilled water, or reverse osmosis water. In other examples, the water may be deionised water or otherwise clean water. Due to the presence of the water, the solution may be referred to as an aqueous solution. The fluid solution is preferably a liquid at room temperature, in particular at 20 °C. Preferably, the fluid solution is a liquid above 0 °C and below 50 °C, preferably at least between 5 °C and 25 °C.

The fluid solution also comprises a thickening agent. The thickening agent is dissolved into the water, and is thus water-soluble. The thickening agent acts to thicken the solution. In other words, the thickening agent increases the viscosity of the solution. The viscosity of the solution is thus higher than liquid water alone. This solution may be referred to as a thickened fluid solution or a viscous fluid. Optionally, the fluid solution may be referred to as a liquid gel.

The fluid solution should be differentiated from solids in which sufficient amounts of thickening agents, gelling agents, or solidifying agents are added to a liquid such that the liquid turns into a solid. In other words, the fluid solution is not a solid. In one example, the fluid solution is not a solid gel. In contrast, the fluid solution is able to flow. In particular, the fluid solution may be able to take on the shape of its container, for example as a liquid.

The thickening agent is dissolved in the water. This should be differentiated from situations where a thickening agent is merely mixed with water. One such example is where the thickening agent comprises a starch-based thickening agent. Starch is insoluble in water at room temperature. Therefore, merely adding starch to water will not provide a thickened fluid solution as described herein. Instead, the starch can be heated to dissolve into the water in a process referred to as starch gelatinization, at which point the starch acts to thicken the water to form a fluid solution.

In the first aspect of the present disclosure, the thickening agent comprises a cellulose-based or a starch-based thickening agent. As used herein, "cellulose-based" preferably connotes a thickening agent comprising cellulose, such as a compound containing cellulose. For example, the thickening agent contains cellulose and is made from cellulose, but may be formed of other atoms or molecules. In other words, the thickening agent may be derived from cellulose. One such example of a cellulose-based thickening agent is hydroxyethyl cellulose. Equally, "starch-based" preferably connotes a thickening agent comprising starch.

In some examples, the suspension contains one or more thickening agents. For example, the suspension may contain a cellulose-based thickening agent and a starch-based thickening agent. In other examples, the thickening agent may consist of a particular thickening agent. For example, the thickening agent may consist of a cellulose-based thickening agent. For example, the thickening agent may consist of a starch-based thickening agent.

Preferably, the thickening agent comprises a cellulose-based thickening agent. Cellulose is a polysaccharide. It is preferable for the cellulose to be obtained from plant material.

More preferably, the thickening agent comprises a cellulose ether. Even more preferably, the thickening agent comprises hydroxyethyl cellulose. For example, the thickening agent may be NatrosolTm hydroxyethyl cellulose, commercially available in powder form from Ashland, USA. Most preferably, the thickening agent comprises NatrosolTM 250 HX or HHX.

Hydroxyethyl cellulose can be dissolved in water at room temperature in order to thicken the fluid solution. As such, it has been found to be a particularly preferable thickening agent.

Optionally, the thickening agent comprises methyl cellulose, such as carboxymethyl cellulose.

Optionally, the fluid solution is viscoelastic. This means that it exhibits both viscous and elastic properties. Optionally, the fluid solution is a shear-thinning fluid. Optionally, the fluid solution is a non-Newtonian fluid. An aqueous solution of hydroxyethyl cellulose is an example of a viscoelastic, shear-thinning, and non-Newtonian fluid.

Optionally, the fluid solution comprises between 1 g and 20 g of hydroxyethyl cellulose per L of water, more preferably between 2 g and 18 g, even more preferably between 3 g and 15 g, and still more preferably between 4 g and 12 g. Even more preferably, the fluid solution comprises between 5 g and 10 g of hydroxyethyl cellulose per L of water. This has been found to provide an optimum viscosity to provide the benefits as described herein. For NatrosolTm HX, preferably the solution comprises between 5 g and 15 g of thickening agent per L of water, more preferably between 8 g and 10 g, most preferably about 9 g per L. For NatrosolTM HHX, preferably the solution comprises between 2 g and 10 g of thickening agent per L of water, more preferably between 5 g and 8 g. At around 20 °C, this provides optimum properties of the suspension, such as the viscosity. For example, providing between 5 and 15 g of hydroxyethyl cellulose per L of water ensures that the Sphagnum is well suspended and flows with the fluid solution and does not float or sink when stored in containers.

Preferably, the thickening agent comprises a starch-based thickening agent. Starch is a polysaccharide. It is preferable for the starch to be obtained from plant material. For example, starch in the form of cornflour is known to be a thickening agent.

As mentioned above, starch can be dissolved in water by heating the starch and water.

Depending on the starch, the mixture can be heated to around 55 °C -85 °C in order to dissolve the starch in the water. This is known as starch gelatinization. By dissolving starch in water in this manner, a thickened fluid solution can be obtained.

Optionally, the fluid solution comprises between 10 g and 100 g of starch per L of water, preferably between 20 g and 50 g, more preferably between 30 g and 40 g. Optionally, the thickening agent comprises a natural starch, such as a maize starch. Optionally, the thickening agent comprises a partially pregelatinized starch. Preferably, the thickening agent comprises between 30 g and 40 g of a partially pregelatinized starch. Optionally, the thickening agent comprises LYCATAB (RIM) PGS, commercially available from Roquette Pharma. Preferably, the thickening agent comprises a pregelatinized starch (i.e. fully pregelatinized). This is soluble is cold water, which is preferable for providing the thickening agent dissolved in water. Optionally, the thickening agent comprises PREGEFLO (RIM) CH 40, commercially available from Roquette Pharma.

Optionally, the thickening agent comprises an extract from a plant. Preferably, the thickening agent comprises an extract from a vascular plant. Vascular plants have conductive (vascular) tissues such as a xylem or phloem, for transporting resources. A vascular plant may otherwise be referred to as a higher plant. Preferably, the term vascular plant excludes non-vascular plants (i.e. lower plants) such as bryophytes and algae. As such, the thickening agent can be made from a plant-based extract from a vascular plant. For example, the plant-based extract may be cellulose or starch.

Optionally, the thickening agent comprises a polymer-based thickening agent. A polymer is a molecule of repeating monomer sub-units. The polymer may be natural or synthetic.

Optionally, the thickening agent comprises a polysaccharide-based thickening agent. A polysaccharide is a carbohydrate polymer chain comprising monomers of monosaccharides bonded together. Starch is an example of a polysaccharide, and is made from a chain of glucose monomers, where glucose is a monosaccharide. A polysaccharide-based thickening agent preferably means a thickening agent derived from or comprising a polysaccharide.

Optionally, the thickening agent comprises a protein-based thickening agent. For example, the thickening agent may comprise gelatine. In another example, the thickening agent may comprise collagen. Optionally, the thickening agent may comprise chickpea water.

Optionally, the thickening agent may comprise guar gum. Optionally, the thickening agent may comprise xanthan gum. Optionally, the thickening agent comprises a fibre-based thickening agent. Optionally, the thickening agent may comprise chia seeds. Optionally, the thickening agent comprises psyllium husk. Optionally, the thickening agent comprises a flour for example coconut flour, chickpea flour, or cornflour.

Optionally, the thickening agent does not comprise sodium alginate. Optionally, the thickening agent does not comprise algin. Algin, or otherwise known as alginic acid, is a polysaccharide. Algin is derived from algae (a non-vascular plant), and is thus not derived from a vascular plant. Algin is often used in salt form as sodium alginate. Sodium alginate is soluble in water, and is often combined with calcium, such as in the form of calcium chloride, to solidify the sodium alginate solution into a solid gel. The calcium forms cross links with the alginate and causes the solution to solidify, and the resultant calcium alginate is not soluble in water. This method is often used for producing solid alginate beads, such as in food preparation. Such a result is not preferable for the suspension of the present disclosure, as this would inhibit the beneficial properties of the fluid solution. In particular, the solid alginate gel would not be a fluid, and would instead set into a solid. In other words, the solid alginate gel would set, and would not be non-setting. Using sodium alginate and calcium chloride would therefore cause setting of the gel, and has been found to form undesirable clumps around the Sphagnum. This prevents a suspension comprising a fluid solution because instead the gel solidifies, meaning the Sphagnum cannot be spread as desired herein.

Moreover, it is preferable to not use sodium alginate in the solution of the present disclosure because the algin clumps and solidifies in the presence of calcium nutrients. In some cases, it may be desirable to add calcium as a nutrient in the suspension, as described in more detail below, in which case sodium alginate would not be desirable for a thickening agent. In some cases, the water used may comprise nutrients such as calcium (such as in ground water which absorbs calcium from soil/rocks), and therefore alginate may solidify when used even where calcium is not specifically added as a nutrient. Furthermore, calcium can often be found in soil where the suspension is spread, and may cause the alginate to solidify. Additionally, alginate has been found to impede growth of Sphagnum as alginate clumps around the Sphagnum and prevents water and air uptake.

Generally, the algin would also react to solidify the solution to form a solid gel in the presence of any divalent cation. Divalent means having a valence of 2. For example, the divalent cation may comprise calcium (i.e. Ca24). In other examples, the divalent cation may comprise magnesium. Other divalent cations include iron (2+), beryllium, strontium, barium, and radium.

It is therefore desirable to select a thickening agent which retains a fluid solution, even in the presence of calcium. A cellulose-based thickening agent or a starch-based thickening agent has been found to be preferable.

Preferably, the thickening agent does not comprise agar. Agar is a solid at room temperature (e.g. 20 °C), and therefore does not provide a fluid solution suitable for use in the present disclosure. Although agar can be used as a solid growth medium, such as during in vitro cultivation of Sphagnum, this does not form a suspension as the Sphagnum merely rests on the solid medium, and the agar does not form a fluid solution.

In cases where the thickening agent is a polymer, the thickening agent optionally comprises a homopolymer. A homopolymer is a polymer comprising repeating monomer units of the same monomer. This is in contrast, for example, to a copolymer which comprises two or more monomers alternating in a chain. Optionally, the homopolymer comprises a glucose monomer. For example, cellulose and starch are homopolymers comprising glucose monomers. As an example, algin is a copolymer and is not a homopolymer.

In cases where the thickening agent is a polymer, each monomer of the thickening agent is optionally electrically neutral. Optionally, each monomer of the thickening agent does not comprise a carboxylate group. For example, cellulose and starch have glucose monomers which are electrically neutral and do not comprise a carboxylate group.

Optionally, the fluid solution also comprises nutrients. The nutrients can be provided to facilitate growth of the Sphagnum within the suspension. The nutrients can be dissolved into the water, and are thus water-soluble. For example, the nutrients may be in the form of nutritional salts. The nutrients can provide conditions which facilitate the growth of Sphagnum. This can be particularly beneficial for fragments of Sphagnum when they initially begin growing. Especially on peatlands where conditions are harsh, providing nutrients to facilitate growth can result in better establishment, especially initially. By providing the nutrients in the suspension, it is not necessary to apply any further nutrients for a set period of time, for example until after the Sphagnum has established and starts growing rapidly (e.g. two to three weeks when grown in a greenhouse, or two to three months when grown in a field). When Sphagnum is grown on a peatland, it is not normally supplemented with nutrients after establishment due to its inaccessibility and therefore providing the initial nutrient supply is especially beneficial. Where Sphagnum is grown for growing media purposes, such as in a greenhouse or in a field, it may be beneficial to treat the Sphagnum at a later stage with irrigation and further nutrients to facilitate growth. In such cases, the nutrients in the suspension provide an initial source.

The nutrients can be mixed throughout the solution leading to uniform access to the nutrients by the Sphagnum. Optionally, the nutrients are homogeneously or substantially homogeneously dispersed throughout the suspension. This facilitates uniformity of growth of the Sphagnum when the suspension is applied over a surface, due to the uniform supply of nutrients.

The thickened solution also retains the nutrients within the suspension. In the absence of a thickening agent, the nutrients would not be well retained, and could be washed away or separated from the Sphagnum. The thickening agent therefore holds the nutrients to provide a supply to the Sphagnum over an extended period of time. Because the nutrients are held in the suspension, this provides an improved system compared to simply providing nutrients via irrigation onto a suspension not comprising nutrients. Applying nutrients, particularly shortly after application of the suspension, can result in washing away the suspension before it is established. It is therefore preferable to not apply irrigation or treatment with nutrients for a period, such as at least two weeks, after application. However, this will depend on the environmental conditions, as for example if it is particularly dry then it may be desirable to apply a small amount of irrigation shortly after application to prevent desiccation of the Sphagnum, but avoid washing away the nutrient. Therefore, providing a source of nutrients can provide an initial supply where it is desirable not to apply nutrients for a period of time after application. Moreover, the nutrient can be absorbed by the Sphagnum and held loosely until required, which can be assisted by avoiding excess irrigation.

Optionally, the nutrients comprise calcium. Calcium has been found to be a particularly useful nutrient for the growth of Sphagnum. Preferably, the nutrients comprise at least 1 mg of calcium per L (litre) of water. Preferably, the nutrients comprise less than 100 mg of calcium per L of water. Preferably, the nutrients comprise between 1.17 mg and 92.39 mg of calcium per L of water. More preferably, the nutrients comprise between 1 mg and 50 mg of calcium per L of water, even more preferably between 2 mg and 25 mg, yet even more preferably between 5 mg and 20 mg. For example, the calcium may be provided in the form of a calcium salt, such as calcium oxide (CaO), calcium chloride (CaCl2), or calcium nitrate (Ca(NO3)2). It has been found that even low levels of calcium can be beneficial to growth of Sphagnum.

The amount of nutrients can be adjusted depending on the amount of Sphagnum in the suspension. For example, the above nutrient levels may be used for 100 g of Sphagnum per L of suspension (100 g calculated using the standardised mass per volume by compressing Sphagnum by 16 g / cm2, which provides a dry weight of around 1.43 g). It will be realised the values disclosed herein are optimised for 100 g of standard weight of Sphagnum, and each value may be scaled to a different concentration for a different amount of Sphagnum.

For example, the nutrients may comprise between 1 mg and 100 mg of calcium per 100 g standardised weight (compressed by 16 g / cm2) of Sphagnum. Optionally, the nutrients may comprise between 0.7 mg and 70 mg of calcium per 1 g dry weight of Sphagnum.

Optionally, the suspension does not solidify in the presence of calcium. In other words, the fluid solution remains fluid in the presence of calcium. For example, calcium may be added to the suspension as a nutrient. In other cases, calcium may be present in the soil to which the suspension is applied. In yet other cases, calcium may be present in the water of the suspension, or water added to the suspension such as irrigation during growth. Therefore, it is preferable for the suspension to remain fluid and not solidify in the presence of calcium, which allows it to be adapted for use in cultivation. Optionally, the suspension does not solidify in the presence of any nutrients, including the nutrients disclosed herein such as magnesium.

Optionally, the nutrients comprise at least one of: magnesium, nitrogen, potassium, and/or phosphorus. For example, this can be in addition or instead of the presence of calcium.

Optionally, the nutrients comprise magnesium. Preferably, the nutrients comprise at least 0.1 mg of magnesium per L of water. Preferably, the nutrients comprise less than 50 mg of magnesium per L of water. Preferably, the nutrients comprise between 0.33 mg and 32.93 mg of magnesium per L of water. More preferably, the nutrients comprise between 1 mg and 15 mg of magnesium per L of water, even more preferably between 3 mg and 10 mg. As above, the amount of magnesium can be adjusted depending on the amount of Sphagnum per L of suspension. Optionally, the nutrients comprise between 0.1 mg and 50 mg of magnesium per 100 g standardised weight (compressed by 16 g / cm2) of Sphagnum. Optionally, the nutrients may comprise between 0.07 mg and 50 mg of magnesium per 1 g dry weight of Sphagnum.

Optionally, the nutrients comprise nitrogen. As used herein, the term "nitrogen" preferably includes nitrogen-containing elements or compounds, and is thus a total nitrogen content of the nutrients present. Preferably, "nitrogen" includes nitrate, ammonium, and ureic nitrogen. Preferably, the nutrients comprise at least 15 mg of nitrogen per L of water. Preferably, the nutrients comprise less than 250 mg of nitrogen per L of water. Preferably, the nutrients comprise between 18.05 mg and 240.81 mg of nitrogen per L of water. More preferably, the nutrients comprise between 15 mg and 100 mg of nitrogen per L of water, even more preferably between 25 mg and 75 mg. As above, the amount of nitrogen can be adjusted depending on the amount of Sphagnum per L of suspension. Optionally, the nutrients comprise between 15 mg and 250 mg of nitrogen per 100 g standardised weight (compressed by 16 g / cm2) of Sphagnum. Optionally, the nutrients may comprise between 10 mg and 175 mg of nitrogen per 1 g dry weight of Sphagnum.

Optionally, the nutrients comprise phosphorus. Preferably, the nutrients comprise at least 5 mg of phosphorus per L of water. Preferably, the nutrients comprise less than 75 mg of phosphorus per L of water. Preferably, the nutrients comprise between 10.99 mg and 61.41 mg of phosphorus per L of water. More preferably, the nutrients comprise between 5 mg and 50 mg of phosphorus per L of water, even more preferably between 10 mg and 20 mg. As above, the amount of phosphorus can be adjusted depending on the amount of Sphagnum per L of suspension. Optionally, the nutrients comprise between 5 mg and 75 mg of phosphorus per 100 g standardised weight (compressed by 16 g / cm2) of Sphagnum.

Optionally, the nutrients may comprise between 3.5 mg and 55 mg of phosphorus per 1 g dry weight of Sphagnum.

Optionally, the nutrients comprise potassium. Preferably, the nutrients comprise at least 20 mg of potassium per L of water. Preferably, the nutrients comprise less than 300 mg of potassium per L of water. Preferably, the nutrients comprise between 66.84 mg and 266.25 mg of potassium per L of water. More preferably, the nutrients comprise between 50 mg and 150 mg of potassium per L of water. As above, the amount of potassium can be adjusted depending on the amount of Sphagnum per L of suspension. Optionally, the nutrients comprise between 20 mg and 300 mg of potassium per 100 g standardised weight (compressed by 16 g / cm2) of Sphagnum. Optionally, the nutrients may comprise between 14 mg and 210 mg of potassium per 1 g dry weight of Sphagnum. The scaling of the nutrients may be applied to other values for each nutrient disclosed herein, wherein each disclosed value corresponds to 100 g of Sphagnum by standardised weight, or 1.43 g by dry weight.

Optionally, the nutrients comprise sulphur. Optionally, the nutrients comprise between 4.30 mg and 65.59 mg of sulphur per L of water.

Optionally, the nutrients comprise iron. Optionally, the nutrients comprise between 0.31 mg and 9.15 mg of iron per L of water.

Optionally, the nutrients comprise sodium, manganese, copper, zinc, boron, molybdenum, and/or chloride. Optionally, the nutrients comprise between 2.51 mg and 53.47 mg of sodium per L of water, between 0.21 mg and 1.94 mg of manganese per L of water, between 0.09 mg and 0.25 mg of copper per L of water, between 0.37 mg and 1.56 mg of zinc per L of water, between 0.14 mg and 1.02 mg of boron per L of water, between 0.01 mg and 0.15 mg of molybdenum per L of water, and/or between 0.16 mg and 97.64 mg of chloride per L of water.

Some nutrients may be present in the soil on which the suspension is spread, and therefore some nutrients may not be necessary to include in the suspension.

Optionally, the suspension does not comprise sugar (e.g. sucrose), vitamins, and/or plant growth hormones.

Optionally, the fluid solution does not solidify for at least 6 hours at a temperature between 5 °C and 25 °C. Preferably, the suspension is non-setting. As used herein, the term "non-setting" preferably means the suspension does not solidify for at least 6 hours, in particular at 20 °C, preferably between 5 °C and 25 °C. Furthermore, preferably the suspension is non-setting in the presence of calcium. In other words, preferably the fluid solution remains a liquid.

The fluid solution generally increases in thickness at a lower temperature but will not solidify until frozen. VVhen a cellulose-based thickening agent such as hydroxyethyl cellulose is used, the solution does not freeze even at 0 °C. At higher temperatures, the solution will become less viscous and above 30 °C it will become very liquid. Above 40 °C it will start to damage the Sphagnum. It is therefore preferable to keep the suspension stored between 0 °C and 30 °C.

As the fragments of Sphagnum are suspended in the solution, the suspension is thus a thickened fluid solution carrying fragments of Sphagnum, which are held in the suspension.

Any suitable Sphagnum species (or optionally a combination thereof) may be used in the present disclosure. As different species of Sphagnum may have different growth requirements, the Sphagnum species for use in the present disclosure may be selected depending on the environment.

The suspension comprises one or more Sphagnum species. Any species could be used, but in one example the present disclosure comprises the use of one or more Sphagnum species selected from the group consisting of: Sphagnum angustifolium, Sphagnum australe, Sphagnum capillifolium, Sphagnum centrale, Sphagnum compactum, Sphagnum cuspidatum, Sphagnum denticulatum, Sphagnum fa//ax, Sphagnum fimbriatum, Sphagnum fuscum, Sphagnum imbricatum (austinii), Sphagnum inundatum, Sphagnum magellanicum (medium), Sphagnum palustre, Sphagnum papillosum, Sphagnum pulchrum, Sphagnum russowii, Sphagnum squarrosum, Sphagnum subnitens, Sphagnum tenellum, and Sphagnum cristatum. In one example, the method comprises the use of one or more Sphagnum species selected from the group consisting of: Sphagnum palustre, Sphagnum 5 capillifolium, Sphagnum capillifolium rube//um, Sphagnum subnitens, Sphagnum denticulatum, Sphagnum squarrosum, Sphagnum fa/lax, Sphagnum fimbriatum, Sphagnum cuspidatum, Sphagnum magellanicum, and Sphagnum papillosum. In one example, the invention comprises the use of one or more Sphagnum species selected from the group consisting of: Sphagnum palustre, Sphagnum capillifolium, Sphagnum capillifolium rubellum, 10 Sphagnum subnitens, Sphagnum squarrosum, Sphagnum magellanicum, and Sphagnum papillosum.

In one example, a Sphagnum species for use in the present disclosure may be one or more selected from the group consisting of: Sphagnum palustre, Sphagnum capillifolium, 15 Sphagnum fallax, Sphagnum magellanicum, Sphagnum papillosum, and Sphagnum squarrosum Most preferably the Sphagnum species is Sphagnum palustre. For example, Sphagnum palustre may be preferable for use in a growing medium because of its physical properties.

It is also envisaged that the invention could be applied to any hybrid Sphagnum species.

Optionally, the plurality of fragments of Sphagnum comprises at least one of the Sphagnum species disclosed herein.

Optionally, the plurality of fragments of Sphagnum comprises at least 2, 3, 4, 5 or more Sphagnum species.

As the plurality of fragments of Sphagnum are suspended in the solution, preferably this means that the fragments of Sphagnum are held dispersed within the solution, and at least some of the fragments of Sphagnum do not float on the solution and/or do not sink beneath the solution. In other words, the fragments are not left behind when the solution is moved, such as by being poured or spread. This means that the Sphagnum can be distributed onto a surface effectively when the suspension is applied. Once the suspension has been applied, the suspension also retains the Sphagnum substantially in position such that the Sphagnum does not fall out of the suspension e.g. by sinking to the bottom. This enables the Sphagnum to be generally fixed in position with the suspension, and not for example to be washed away while the rest of the suspension is left behind. This also allows the Sphagnum to absorb the nutrients in the suspension over an extended period of time, which is particularly advantageous for the initial period of growth after application, where nutrient supply can aid establishment.

Optionally, the fragments of Sphagnum are dispersed evenly throughout the suspension. For example, the Sphagnum may be homogeneously spread out in the solution (i.e. uniform density). In some cases, it can be acceptable to provide a thoroughly mixed suspension, for example such that at least 50% of the fragments are not settled at the bottom or the top of the mixture.

The thickening agent provides properties of the suspension that help retain the Sphagnum itself within the suspension. By thoroughly mixing the Sphagnum within the solution of the suspension, the Sphagnum can be distributed throughout the suspension, and the viscosity of the solution will retain the Sphagnum. In the absence of a suitable thickening agent, the Sphagnum would not be held suspended by the water alone, and instead would sink to the bottom of the solution. Equally, the thickening agent and quantity thereof is preferably chosen to enable sufficient mixing. If the viscosity is too high, the Sphagnum will not mix in well, may float on top of the upper surface of the thick solution or may be damaged by the mixing.

Preferably, the suspension does not clump around the Sphagnum. By suitable choice of the thickening agent as described herein, the suspension will not clump around the Sphagnum and/or around the nutrients.

A cellulose-based thickening agent or a starch-based thickening agent acts as a suitable thickening agent which provides the above benefits.

The suspension can be used to provide a more uniform density of Sphagnum when spread over a surface than conventional techniques. In particular, the Sphagnum can be mixed to create a substantially uniform density of Sphagnum within the suspension and, because the suspension can retain the Sphagnum well, when the suspension is applied to the surface, the density can be preserved. The thickening agent aids this retention when compared to thinner, less viscous liquids such as in the absence of a thickening agent dissolved in the fluid solution The suspension can provide a uniform density of Sphagnum which can be applied more evenly than conventional techniques. For example, a substantially uniform density of Sphagnum can be applied by spraying, compared to planting individual clumps of Sphagnum over an area. Moreover, the coverage of a surface can be increased more easily, as the suspension can be spread to entirely cover a surface. The density of Sphagnum within the suspension can then be controlled to provide the desired initial coverage of Sphagnum over the area.

The thickened solution is preferably sticky. This allows the suspension to stick to the surface to which it is applied and provides a securing means to secure the suspension to the surface. This can alleviate the problem of unsecured Sphagnum blowing away or being washed away by rain. Instead, the suspension can retain the Sphagnum in position for long enough to provide nutrients to the Sphagnum during its initial establishment phase and long enough that the Sphagnum becomes established enough to continue growing.

Optionally, the suspension is adhesive to a growing substrate. Optionally, the suspension is adhesive to a growing substrate comprising soil, sand, compost, peat, and/or dried Sphagnum. This means the suspension sticks to the substrate when it is put into contact with it. For example, the substrate may be a peat surface or compost in a tray. This allows the suspension to retain the Sphagnum on the surface and anchor it.

Optionally, the suspension provides capillary contact with a surface to which it is applied to enable fluid transfer between the surface and the suspension. As used herein "capillary contact" preferably means providing a fluid pathway, such as through capillary action. In this manner, the suspension can contact the surface to which it is applied, and provide a fluid pathway between the surface and the Sphagnum, thereby enabling fluid transfer. For example, when applied to a peat surface, the suspension can enable the transfer of water and nutrients from the peat into the solution for use by the Sphagnum in growth. The capillary contact is promoted by the thickened fluid solution because the surface area in contact with the surface is increased compared to a solid (e.g. a solid gel) due to its flowing properties. The thickened suspension also retains that contact over time, which is advantageous compared to liquid without an appropriate thickening agent, which instead would wash or drain away. A cellulose-based or a starch-based thickening agent has been found to provide optimum capillary contact and promote fluid transfer. Using alternative thickening agents such as alginate has been found to inhibit fluid transfer and prevent air and water uptake.

The fluid solution in the suspension allows the suspension to be spread over a surface, for example by spraying. This allows vast areas to be covered quickly, especially when the spraying can be conducted by a machine or a hand-held sprayer, and much faster than applying clumps of Sphagnum by hand. Spraying also allows for much easier application than a solid suspension.

By providing fragments of Sphagnum, the Sphagnum can be sprayed more easily than long strands which have been found to clog and tangle in a spraying system. Preferably, by having fragments of length less than 50 mm, more preferably between 5 mm and 30 mm, the fragments are easily sprayed and avoid clogging or tangling.

Optionally, the suspension is capable of being sprayed through a nozzle having a diameter of between 5 mm and 10 mm. Thus, the viscosity of the suspension is provided to enable such spraying. If the suspension is too viscous, for example if it comprises a more solid gel, the suspension would not be able to be sprayed through such a nozzle. It is preferable for the suspension to be capable of being sprayed in this way, while retaining the Sphagnum dispersed in the solution. This provides an ideal viscosity, where if the viscosity is too high such as with a solid gel, the suspension could not be sprayed, and where if the viscosity is too low such as with a non-thickened solution, the suspension could not retain the Sphagnum and the Sphagnum would not be carried with the solution. Therefore, the thickened fluid solution of the present disclosure provides an optimised suspension for holding Sphagnum for applying to a surface.

Optionally, the fluid solution has a viscosity of between 1000 and 4000 mPas at 25 °C. As used herein, the viscosity is measured using the standard Brookfield scale, such as using a Brookfield Dial Reading Viscometer LVF, commercially available from Brookfield Engineering Laboratories, Inc, USA.

Optionally, the fluid solution has a pH of between 3.5 and 6.5, preferably between 5.0 and 30 6.0.

To provide a sustainable way of cultivating Sphagnum, it is preferable that the source is not translocated from a wild site. For example, when applying Sphagnum to restore a damaged peatland or for production of growing media, it is clearly preferable that the Sphagnum used is not translocated from another wild site such as a peatland. Preferably, the Sphagnum is sourced from a cultivated source. In one example, the Sphagnum could be cultivated or grown in a greenhouse. In some examples, this could originate from a wild site, but the Sphagnum can be bulked up by cultivation in a greenhouse, meaning that more Sphagnum can be used for a certain amount taken from the wild site.

Optionally, the fragments of Sphagnum are cultivated in vitro. This means that the Sphagnum has been propagated under controlled laboratory conditions, such as in a culture vessel such as a petri dish, a test tube, or other sterile container. Preferably, the in vitro Sphagnum has been cultivated using tissue culture techniques. Preferably, the fragments of Sphagnum are micropropagated. This means that the Sphagnum was grown using clonal tissue culture techniques to produce genetically identical plantlets. This is typically performed in a laboratory by initiating Sphagnum in vitro. This can require only a small amount of wild harvested material, reducing the environmental impact. The in vitro Sphagnum can then be chopped to form the plurality of fragments of Sphagnum for use in the suspension of the present disclosure.

Optionally, the fragments of Sphagnum have subsequently been cultivated in vivo. As used herein, cultivating "in vivo" preferably means cultivating outside of laboratory conditions, such as outside of in vitro or tissue culture conditions. For example, after growing in vitro such as by micropropagation, the Sphagnum may be transferred to and grown in vivo such as in a greenhouse. Preferably, the Sphagnum is cultivated in vivo for at least one month, preferably at least four months. The Sphagnum can then be harvested and chopped to form the plurality of fragments of Sphagnum for use in the suspension of the present disclosure.

Providing fragments of Sphagnum may involve chopping strands of Sphagnum to provide the desired length. For example, a desired length may be between 5 mm and 30 mm.

Optionally, at least 50% by mass of the fragments of Sphagnum have a length of at least 5 mm. During the chopping process, smaller fragments may form due to inefficiencies in the chopping mechanism and the tendency of branches and leaves to break off due to the fragility of the strands. By suitable choice of a chopping mechanism, this effect can be mitigated, but a small proportion of the fragments will have a length shorter than desired. For example, it is desirable to minimise the number of fragments with a length less than 5 mm. These fragments tend to have fewer potential growing points, are less sturdy, and have a lower probability of quality establishment. Preferably, less than 60% by mass of the fragments of Sphagnum in the suspension will have a length less than 5 mm, more preferably less than 50%, still more preferably less than 40%, yet more preferably less than 35%. Measurements of the number of fragments less than 5 mm are shown in Example 2 below.

Optionally, the fragments of Sphagnum have a mean length of between 5 mm and 50 mm, preferably between 5 mm and 30 mm. Optionally, fragments of Sphagnum having a length of at least 5 mm have a mean length of between 5 mm and 30 mm. Optionally, fragments of Sphagnum having a length of at least 5 mm have a mean length of between 5 mm and 50 mm. Preferably, the fragments of Sphagnum having a length of at least 5 mm have a mean length of between 5 mm and 25 mm, more preferably between 7.5 mm and 20 mm, even more preferably between 7.5 and 15 mm. Measurements of the lengths of fragments of Sphagnum are shown in Example 3 below.

Optionally, the suspension comprises at least 1000 fragments of Sphagnum having a length of at least 5 mm per L of fluid solution. Preferably, the suspension comprises at least 2000 fragments of Sphagnum having a length of at least 5 mm per L of fluid solution. Measurements of the numbers of fragments of Sphagnum are shown in Example 2 below.

Optionally, the suspension comprises a total mass of fragments of Sphagnum of at least 50 g per L of fluid solution. Optionally, the suspension comprises a total mass of fragments of Sphagnum of between 50 g and 100 g per L of fluid solution, preferably about 100 g per L. Optionally, the suspension comprises a total mass of fragments of Sphagnum of at least 25 g per L of fluid solution. Optionally, the suspension comprises a total mass of fragments of Sphagnum of less than 500 g per L of fluid solution. Optionally, the suspension comprises a total mass of fragments of Sphagnum of less than 400 g per L of fluid solution, preferably less than 300 g per L. The total mass can be calculated by compressing the Sphagnum to a standardised mass per volume by compressing with a force of 16 g / cm2 to remove excess water to ensure a standardised mass.

Optionally, the suspension comprises a total dry mass of fragments of Sphagnum of at least 1 g per L of fluid solution. The dry mass can be calculated by drying the Sphagnum to remove the water. As is standard in the art, the drying can be performed by heating the Sphagnum at around 110 °C for at least 24 hours. Alternatively, the drying can be performed by drying at around 25 °C in a humidity of less than 50 % until no further weight loss is measured. This ensures that all water has been evaporated, and that remaining is the dry mass of the Sphagnum. Of course, this will kill the Sphagnum, so this merely provides a useful method for calculation of the dry mass. Measurements of the total dry mass of fragments of Sphagnum are shown in Example 5 below.

Optionally, the fragments of Sphagnum have a mean stem diameter of between 0.1 mm and 1 mm. Measurements of the stem diameter of fragments of Sphagnum are shown in Example 4 below.

According to a second aspect of the present disclosure, there is provided a method of producing a suspension of Sphagnum, the method comprising: providing a plurality of fragments of Sphagnum; preparing a fluid solution comprising: providing water; and dissolving a thickening agent in the water, wherein the thickening agent comprises a cellulose-based or a starch-based thickening agent; and mixing the plurality of fragments of Sphagnum with the fluid solution to suspend the plurality of fragments of Sphagnum in the fluid solution.

Disclosed herein is a method of producing a suspension of Sphagnum, the method comprising: providing a plurality of fragments of Sphagnum; preparing a fluid solution comprising: providing water; and dissolving a thickening agent in the water; and mixing the plurality of fragments of Sphagnum with the fluid solution to suspend the plurality of fragments of Sphagnum in the fluid solution. Optionally, the suspension further comprises nutrients. Optionally, the nutrients comprise calcium. Optionally, the thickening agent comprises a cellulose-based thickening agent. Optionally, the thickening agent comprises a starch-based thickening agent.

Optionally, the method of the second aspect comprises providing the suspension comprising any of the features described in relation to the suspension of the first aspect.

Features described in relation to the first aspect may be applied to the second aspect alone or in combination, and vice versa. In particular, features of the fragments of Sphagnum, the nutrients, and the thickening agent described in relation to the first aspect can be applied to the second aspect, and vice versa.

According to a third aspect of the present disclosure, there is provided a method of providing a seedstock of Sphagnum comprising: providing in vitro Sphagnum; applying the Sphagnum to a growth surface; cultivating the Sphagnum in vivo on the growth surface; harvesting the cultivated Sphagnum from the growth surface; and chopping the harvested Sphagnum to provide seedstock of Sphagnum for cultivation, the seedstock comprising a plurality of fragments of the in vivo Sphagnum.

Disclosed herein is a method comprising providing in vitro Sphagnum; applying the Sphagnum to a growth surface; cultivating the Sphagnum in vivo on the growth surface; harvesting the cultivated Sphagnum from the growth surface; and chopping the harvested Sphagnum to provide a plurality of fragments of in vivo Sphagnum for cultivation.

The Sphagnum may be any one or more of the species of Sphagnum disclosed herein. Preferably, the Sphagnum is Sphagnum palustre.

The Sphagnum is in vitro. This means that the Sphagnum has been propagated under controlled laboratory conditions. Preferably, the in vitro Sphagnum has been cultivated using tissue culture techniques. Preferably, the Sphagnum has been micropropagated. This means that the Sphagnum was grown using clonal tissue culture techniques to produce genetically identical plantlets. Preferably, the in vitro Sphagnum has been cultured under sterile conditions or substantially sterile conditions.

The term "in vitro Sphagnum" encompasses Sphagnum which has been grown under in vitro conditions, such as in a laboratory. The in vitro Sphagnum is in contrast to in vivo Sphagnum which has been grown outside of controlled laboratory conditions, such as in a greenhouse or on a field. Growing in vitro isolates the Sphagnum and reduces exposure to pathogens, resulting in a cleaner product. Clonal propagation in vitro can produce identical plantlets from the original source.

Preferably, the in vitro Sphagnum has not been grown in vivo, e.g. outside of a laboratory such as in a greenhouse, before being applied to the growth surface. In other words, the term "in vitro Sphagnum" preferably does not include Sphagnum that has been cultivated outside of laboratory conditions, for example for more than one month, before being applied to the growth surface. However, it will be appreciated that the in vitro Sphagnum may be briefly removed from in vitro conditions, such as during the preparation of a suspension comprising the in vitro Sphagnum as will be described below, for example by removing the Sphagnum from the laboratory for chopping into fragments and mixing with a fluid solution to form a suspension of in vitro Sphagnum, and that the term "in vitro Sphagnum" thus preferably extends to this brief period outside of laboratory conditions.

In this manner, the in vitro Sphagnum is clean. The Sphagnum can be grown in a nutrient medium and for example supplemented with artificial lighting to culture the Sphagnum in vitro. The Sphagnum may be grown in culture vessels such as petri dishes which are sealed or substantially sealed to prevent contamination. The exposure of the in vitro Sphagnum to contaminants is thus minimal. The cultures and/or equipment may also be sterilised before initiation, reducing the risk of contamination and ensuring a clean product.

Providing micropropagated in vitro Sphagnum allows clonal production which provides a sustainable source, with less damage to the environment than harvesting wild Sphagnum. A particular desired species can also be selected, for example selecting particular species and/or from a particular location which can be important for restoration and reintroduction purposes. In other cases, particular species that have desirable growth characteristics for Sphagnum farming may be selected. For example, Sphagnum palustre has been found to be particularly well suited for its physical properties and produces a high productivity harvest and a high volume growing media.

The Sphagnum can be applied to a growth surface. The growth surface is a surface suitable for facilitating growth of the Sphagnum. For example, the growth surface may comprise a growth substrate such as compost, soil, sand, peat, dried Sphagnum, and/or alternative growing media.

The Sphagnum can then be cultivated in vivo on the growth surface. As used herein, "cultivating" preferably means maintaining the Sphagnum in a live state. Cultivating encompasses and preferably refers to facilitating growth of the Sphagnum. Preferably, the method comprises growing the Sphagnum in vivo on the growth surface. As used herein, cultivating "in vivo" preferably means cultivating outside of in vitro conditions, or in other words outside of controlled laboratory conditions. For example, in vivo cultivating encompasses cultivating in a greenhouse or in a field.

The Sphagnum can then be harvested from the growth surface. After a desired period of time cultivating the Sphagnum, the Sphagnum can be removed from the growth surface. This may be determined by the length of time, or by the size or coverage of the Sphagnum after a sufficient amount of growth.

The harvested Sphagnum can then be chopped to provide a seedstock of Sphagnum for cultivation, the seedstock comprising a plurality of fragments of the in vivo Sphagnum. In other words, the harvested in vivo Sphagnum is chopped into a plurality of fragments which form the seedstock. The purpose of the chopping is to provide fragments of a desired size for further growth. Preferably, the chopped fragments are still alive and capable of growth.

Preferably, the harvested Sphagnum is alive when chopped. In particular, the harvested Sphagnum is preferably not dried out or dead before chopping.

The plurality of fragments of in vivo Sphagnum thus provide the seedstock. The seedstock acts as a supply of Sphagnum for further growth. For example, the seedstock can be used by spreading the fragments on a growth surface for growth. In other words, the term "seedstock" does not mean seeds per se, but rather vegetative pieces of Sphagnum which can be used for further growth. As Sphagnum can be grown from fragments of vegetative material, fragments can be used as a seedstock. The seedstock can also include a suspension of Sphagnum as will be described below, which can act as a particularly effective means for spreading the Sphagnum.

The resultant in vivo Sphagnum in the seedstock is particularly suitable for further growth. Because it originated from an in vitro source, it retains the properties of that source. For example, the material is clonal and its propagation is sustainable, and it is still relatively clean given appropriate in vivo conditions of cultivation.

Furthermore, the resultant fragments of in vivo Sphagnum in the seedstock have significantly different characteristics to the in vitro Sphagnum.

Sphagnum typically grows from individual discrete growing points called innovations. In whole plants of wild Sphagnum, each strand grows primarily from a large capitulum at the top of the stem. This results in a very small number of growing points in a defined area of Sphagnum, largely constrained by the number of capitula. In vitro Sphagnum forms a long thin strand with a small capitulum and without any branches. An example strand of in vitro Sphagnum is shown in Figure 1. The stem comprises small leaves spaced around 0.5 -1 mm apart along the length of the stem. If in vitro Sphagnum is used as a seedstock, many of the advantages of in vitro material are realised e.g. a clean and sustainable product. Furthermore, it has been found that if in vitro Sphagnum is chopped into a plurality of fragments for the seedstock, each leaf has the potential to form an innovation. In other words, each leaf is a potential innovation. On each fragment, one or more leaves will typically form an innovation and begin to grow when applied to a growth surface. This results in a large number of innovations compared to only the capitulum growing. Providing a large number of innovations is useful in a seedstock as it increases the initial coverage of Sphagnum. However, the inventors have found that the seedstock can be further improved by cultivating the in vitro Sphagnum in vivo, and then using the subsequent material as the seedstock.

In contrast to in vitro Sphagnum, the resultant strands of in vivo Sphagnum are physically different. As example strand of in vivo Sphagnum is shown in Figure 2. The in vivo Sphagnum comprises a number of branches along the length of the stem, more similar to wild Sphagnum. The branches are typically arranged around 5 mm apart along the length of the stem. \Mien the in vivo Sphagnum is chopped and the seedstock is applied to a growth surface, the branches can form innovations. In other words, the branches are potential innovations, rather than the leaves. By chopping the fragments to generally provide around one branch per fragment, a potential innovation can be provided per fragment. This results in a slightly lower number of total innovations compared to the same amount of in vitro material. Therefore, it might be expected that the growth performance would be worse.

However, surprisingly, the in vivo Sphagnum provides an improvement of growth compared to the in vitro Sphagnum, especially over the initial establishment growth phase.

It has been found that the resulting fragments of in vivo Sphagnum are more robust than the in vitro Sphagnum. In particular, the stem diameter of the fragments is much larger than the diameter of the stems of in vitro Sphagnum. This allows for better water and nutrient absorption and transport, which promote better growth when the in vivo seedstock is applied to a growth surface. The thicker stems provide a stronger material which is more resilient to drought and contaminants, and has better water holding capacity.

It has also been found that when the in vivo Sphagnum in the seedstock is applied to a growth surface, the size of each innovation is also much larger than the size of the innovations of the in vitro Sphagnum. As each individual growing point is much larger, this means that it is more hardy and resilient when further cultivated. This makes it particularly well-suited for initial establishment, especially in harsh conditions such as on a peatland.

The in vivo Sphagnum originating from the in vitro source still has a higher density of innovations than wild-harvested Sphagnum. The cultivation phase of the present method hence results in the Sphagnum becoming more adapted to the environment, so that when the seedstock is applied to a surface and grown on, it establishes faster and more effectively. The larger innovations are less dominated by competitors such as algae, other mosses, or liverworts, and result in improved efficiency of establishment over in vitro Sphagnum. The thicker stems provide an improved water holding capacity which can improve establishment in poor conditions. The in vivo Sphagnum of the seedstock thus provides an optimum between the number of innovations from the in vitro material, and the larger and more robust innovations from cultivating in vivo.

The in vivo fragments are faster to establish because of their larger growing points. The fragments are also more resistant to the environmental conditions due to their exposure during the cultivating step of the method. This also avoids the effect of shock to the in vitro Sphagnum after immediate spreading directly from the laboratory.

Measurements of the comparative growth of fragments of in vitro Sphagnum and fragments of in vivo Sphagnum are shown in Example 1 below.

Optionally, the fragments of in vivo Sphagnum have a mean stem diameter which is at least 50% thicker than a mean stem diameter of the in vitro Sphagnum. Optionally, the fragments of in vivo Sphagnum have a mean stem diameter which is at least 20% thicker than a mean stem diameter of the in vitro Sphagnum, preferably at least 30% thicker, more preferably at least 40%, still more preferably at least 50%, even more preferably at least 60%, yet more preferably at least 70%.

Optionally, the fragments of in vivo Sphagnum have a mean stem diameter of at least 0.4 mm. Optionally, the in vitro Sphagnum has a mean stem diameter of less than 0.3 mm.

Measurements of the mean stem diameter are shown in Example 5 below.

Optionally, the fragments of in vivo Sphagnum have a mean length of between 5 and 50 mm. Optionally, the fragments of in vivo Sphagnum have a mean length of between 5 and 30 mm, preferably between 10 and 20 mm. It is preferable to maximise the number of fragments of in vivo Sphagnum with a length of at least 5 mm to maximise the number of fragments with a branch with the potential to form an innovation.

Optionally, the providing in vitro Sphagnum comprises chopping in vitro Sphagnum into a plurality of fragments of in vitro Sphagnum. The in vitro Sphagnum fragments may have any length described in relation to the in vivo Sphagnum. For example, the fragments of in vitro Sphagnum may have a mean length of between 5 and 50 mm, preferably between 5 and 30 mm.

Optionally, the providing in vitro Sphagnum comprises mixing the plurality of fragments of in vitro Sphagnum with a first fluid solution to provide a suspension of in vitro Sphagnum. For example, the fragments of in vitro Sphagnum may be mixed with the first fluid solution by stirring with a mechanical stirrer, or by stirring with a utensil operated by hand.

Optionally, the first fluid solution comprises water and a thickening agent dissolved in the water. The suspension may comprise any of the features of the suspension described herein, such as in the first aspect of the present disclosure. For example, the first fluid solution may comprise any of the features described in relation to the fluid solution of the first aspect of the present disclosure. For example, the thickening agent may comprise a cellulose-based or a starch-based thickening agent. For example, the fluid solution may comprise nutrients as described above.

Optionally, the suspension of in vitro Sphagnum comprises at least 50 g of in vitro Sphagnum per L of the first fluid solution. Optionally, between 50 g and 150 g, preferably around 100g.

Optionally, the applying comprises applying the suspension of in vitro Sphagnum onto the growth surface at a density of between 0.5 and 5 L / m2. Preferably, the applying comprises applying the suspension of in vitro Sphagnum onto the growth surface at a density of between 0.5 and 3 L / m2. More preferably, the applying comprises applying the suspension of in vitro Sphagnum onto the growth surface at a density of between 1 and 3 L / m2. Even more preferably, the suspension is applied onto the growth surface between 1.5 and 2.5 L / m2, most preferably approximately 2 L/ m2.

Optionally, the applying comprises spraying the suspension of in vitro Sphagnum onto the growth surface Optionally, the spraying comprises pumping the suspension of in vitro Sphagnum through an aperture to hit a deflecting plate. Preferably, the aperture is less than 10 mm in diameter, more preferably between 5 mm and 10 mm, most preferably about 6 mm. The deflecting plate provides spreading over a greater area and a more even spread. For example, the suspension may be sprayed by using a peristaltic pump. A peristaltic pump evenly pumps the suspension without the fragments of Sphagnum tangling in the pump. For fragments sizes below about 30 mm, a peristaltic pump has been found to provide optimum pumping without tangling. Alternatively, the pump may be a vane pump having smooth soft vanes to prevent damage to the Sphagnum.

Optionally, the applying comprises applying the suspension in discrete amounts onto the growth surface. For example, the suspension may be pumped onto individual cells in a plug cell tray. This results in small clumps of Sphagnum growing on individual plugs of the growth surface, such as a growing medium.

Optionally, the growth surface comprises compost, soil, sand, peat, and/or dried Sphagnum. Alternatively, the growth surface may comprise an alternative growing media, such as a peat-free growing media. Preferably, the growth surface comprises peat and/or dried Sphagnum. The growth surface is preferably the uppermost surface, for example exposed to the air (before Sphagnum is applied). For example, the growth substrate may be a growing medium such as compost, peat, dried Sphagnum, and/or alternative growing media. The growth surface may be an outdoor area such as a field or a peatland. Alternatively, the growth surface may be a horticultural growing bed, such as an indoor or outdoor bed. The growth surface may be arranged in a tray, such as a horticultural tray for use in a greenhouse or polytunnel or outdoors. For example, the growth surface may comprise a horticultural growing medium (e.g. a peat-based growing medium) applied to a tray. The tray may be a plug cell tray having individual cells for plugs, or it may be a carpet tray having a single larger region for growing media.

Optionally, the cultivating is carried out under controlled environmental conditions. Optionally, the controlled environmental conditions controlled comprise at least one of: irrigation, nutrient supply, lighting, shading, temperature, and/or humidity. For example, the cultivating may be carried out in an indoor growing room such as a greenhouse or a polytunnel. Optionally, the indoor growing room is heated. Optionally, the indoor growing room comprises artificial lighting to replace or supplement natural lighting for growth of Sphagnum.

Optionally, the cultivating comprises irrigating the Sphagnum. Optionally, the irrigating comprises applying water to the Sphagnum at a rate of at least 1 L / m2 / day. Optionally, the irrigating is performed using spray irrigation such as using small droplet sprinklers or a misting system. The amount of irrigation can be dependent on water loss by evaporation. In other words, humidity airflow can affect the water application required, and more or less water can be applied as necessary.

Optionally, the cultivating comprises applying nutrients to the Sphagnum. Optionally, the nutrients are contained in water supplied via irrigation. For example, the nutrients may be the same or different to nutrients provided in the suspension. Optionally, the nutrients are applied after cultivating for at least two weeks. For example, as the suspension contains a supply of nutrients, in some cases it is not necessary to supply nutrients for an initial period of cultivation. The nutrients may comprise any of the nutrients in any of the concentrations or ranges as described in relation to the first or second aspect of the present disclosure.

Optionally, the cultivating is carried out for at least one month. Preferably, the cultivating is carried out for at least four months. More preferably, the cultivating is carried out for between four and six months. In some cases, the cultivating can be carried out for longer, such as 5 when outside and the Sphagnum growth is slower.

Optionally, the chopping the harvested Sphagnum comprises chopping such that at least 50 % by weight of the fragments of in vivo Sphagnum have a length of at least 5 mm. Optionally, at least 30 %, preferably at least 40 %, more preferably at least 50 % even more preferably at least 60 %.

Optionally, the providing the seedstock of Sphagnum comprises mixing the plurality of fragments of in vivo Sphagnum with a second fluid solution to provide a suspension of in vivo Sphagnum. For example, the second fluid solution may be the same as the first fluid solution. In other cases, the second fluid solution may be different. For example, it may have a different thickening agent or concentration, or a different nutrient composition. Although referred to as the "second" fluid solution, in some examples the seedstock may comprise a fluid solution, while the in vitro Sphagnum is not suspended in a fluid solution. In other words, the second fluid solution is not limited to the inclusion of the first fluid solution.

Optionally, the second fluid solution comprises water and a thickening agent dissolved in the water. For example, the second fluid solution may comprise any of the features of the fluid solution disclosed herein, such as in the first aspect of the present disclosure. For example, the thickening agent may comprise a cellulose-based or a starch-based thickening agent.

For example, the second fluid solution may comprise nutrients such as calcium. Preferably, the second fluid solution comprises hydroxyethyl cellulose as a thickening agent. In some examples, the second fluid solution comprises more thickening agent than the first fluid solution. In one example, the second fluid solution comprises around 0.5 g more of hydroxyethyl cellulose per L of water.

In other words, the seedstock comprises the suspension of Sphagnum. As described herein, the suspension provides a particularly effective means for carrying the Sphagnum and for spreading it.

Optionally, the method further comprises applying the seedstock of Sphagnum to a growth surface. The growth surface may comprise any of the features described above in relation to the growth surface on which the first suspension is applied. For example, applying the seedstock of Sphagnum to a growth surface may comprise spraying the suspension of in vivo Sphagnum on the growth surface.

Optionally, the method further comprises cultivating the Sphagnum in the seedstock in vivo on the growth surface. The cultivating may comprise any of the features described above in relation to cultivating the in vitro Sphagnum in vivo on the growth surface.

Optionally, the method further comprises harvesting the Sphagnum of the seedstock from the growth surface.

Optionally, the method comprises providing a growing medium comprising the harvested Sphagnum of the seedstock, comprising drying the harvested Sphagnum of the seedstock.

Optionally, the method further comprises chopping the harvested Sphagnum from the seedstock to provide a second seedstock of Sphagnum for cultivation, the second seedstock comprising a plurality of fragments of second generation in vivo Sphagnum.

Optionally, the providing the second seedstock comprises mixing the plurality of fragments of second generation in vivo Sphagnum with a third fluid solution to provide a suspension of second generation in vivo Sphagnum. The third fluid solution may comprise any of the features described above in relation to the first or second fluid solutions, or the fluid solution of the first aspect of the present disclosure.

This third suspension can then be cultivated such as for producing growing media as described above.

According to a fourth aspect of the present disclosure, there is provided Sphagnum obtainable by the method according to any one of the preceding claims. As will be appreciated, the physical difference between the in vitro Sphagnum and the resulting in vivo Sphagnum of the seedstock represents a significant and detectable technical alteration imparted by the process.

Aspects of the invention may be provided in conjunction with each other and features of one aspect may be applied to other aspects. Any feature in one aspect of the invention may be applied to other aspects of the invention, in any appropriate combination. It should also be appreciated that particular combinations of the various features described and defined in any aspects of the invention can be implemented and/or supplied and/or used independently.

Embodiments related to the method may be applied to the Sphagnum obtainable by the method, and vice versa.

Other definitions of terms may appear throughout the specification. Before the exemplary embodiments are described in more detail, it is to be understood that this disclosure is not limited to particular embodiments described, and as such may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting, since the scope of the present disclosure will be defined only by the appended claims.

Where a range of values is provided, it is understood that each intervening value, to the tenth of the unit of the lower limit unless the context clearly dictates otherwise, between the upper and lower limits of that range is also specifically disclosed. Each smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in that stated range is encompassed within this disclosure. The upper and lower limits of these smaller ranges may independently be included or excluded in the range, and each range where either, neither or both limits are included in the smaller ranges is also encompassed within this disclosure, subject to any specifically excluded limit in the stated range. Where the stated range includes one or both of the limits, ranges excluding either or both of those included limits are also included in this disclosure.

Embodiments of the disclosure are described below, by way of example only, with reference to the accompanying Figures and Examples.

Figure 1 shows a photograph of a strand of in vitro Sphagnum.

Figure 2 shows a photograph of a strand of in vivo Sphagnum.

Figure 3 shows a table of the number of innovations counted when Sphagnum was applied to a surface.

Figure 4 shows a photograph of coverage of Sphagnum for Trial 1 Figure 5 shows a photograph of coverage of Sphagnum for Trial 2.

Figure 6 shows a photograph of coverage of Sphagnum for Trial 3 Figure 7 shows a table of the proportion of fragments of Sphagnum having a length of at least 5 mm for Trial 1.

Figure 8 shows a table of the proportion of fragments of Sphagnum having a length of at least 5 mm for Trial 2.

Figure 9 shows a table of the proportion of fragments of Sphagnum having a length of at least 5 mm for Trial 3.

Figure 10 shows a table of the lengths of fragments of Sphagnum having a length of at least mm for Trial 1.

Figure 11 shows a table of the lengths of fragments of Sphagnum having a length of at least 5 mm for Trial 2.

Figure 12 shows a table of the lengths of fragments of Sphagnum having a length of at least 5 mm for Trial 3.

Figure 13 shows a table of the stem diameters of Sphagnum for Trial 1.

Figure 14 shows a table of the stem diameters of Sphagnum for Trial 2.

Figure 15 shows a table of the stem diameters of Sphagnum for Trial 3.

Figure 16 shows a table of the wet weight, dry weight, and water content of fragments of Sphagnum for Trial 1.

Figure 17 shows a table of the wet weight, dry weight, and water content of fragments of Sphagnum for Trial 2.

Figure 18 shows a table of the wet weight, dry weight, and water content of fragments of Sphagnum for Trial 3.

Figure 19 shows a table of the distance between branches for Trial 1.

Figure 20 shows a table of the distance between branches for Trial 2.

Figure 21 shows a table of the distance between branches for Trial 3.

EXAMPLES

EXAMPLE 1

Suspensions of Sphagnum Materials and Methods Suspensions of Sphagnum were prepared in accordance with the present disclosure. Three trials were performed. Trial 1 was in vitro Sphagnum taken immediately from the laboratory after 3 months of growth. Trial 2 was first generation in vivo Sphagnum harvested after 6 months of growth in a heated indoor greenhouse, where in vitro Sphagnum was applied to produce the in vivo Sphagnum. Trial 3 was also first generation in vivo Sphagnum harvested after 6 months of growth in a heated indoor greenhouse, but the Sphagnum was unchopped.

The strands of Sphagnum of Trial 1 and 2 were chopped into typical lengths of around 5 to 30 mm by a machine. In Trial 3, the strands were not chopped, and had a typical length of around 50-100 mm.

A fluid solution was then prepared by preparing a nutrient stock solution. Hortimix 15-5-15 was used, commercially available from Hortifeeds, UK. The nutrients were then diluted in water to provide 0.75 g of Hortimix 15-5-15 per L of water.

A thickening agent was then dissolved in the nutrient solution. The thickening agent was hydroxyethyl cellulose, in the form of NatrosolTM HX, commercially available from Ashland, USA. 8 mg of hydroxyethyl cellulose was used per L of solution.

The fragments of Sphagnum of each trial were then mixed with the solution. This suspended the fragments of Sphagnum within the thickened solution.

The suspension was then sprayed onto a surface at an application rate of 2 L per m2 and cultivated for 4 weeks. Irrigation was supplied by applying water to keep damp, and no further nutrients were applied.

A photograph was taken of each of the three trials. A standardising ring was placed on the Sphagnum surface in the photograph for calibrating size. By measuring the size of the ring in the photograph, an adjustment ratio was calculated for calibration. The width and length of the photograph was then measured for each trial, and these values were multiplied together to provide a measured area for each photograph. This area was then multiplied by the adjustment ratio to provide the true area in the photograph.

The number of innovations were then counted in each trial. In particular, the number of small innovations and the number of large innovations were counted individually to provide a total number of innovations. Small innovations were defined as those lacking significant branches. By dividing the number of innovations by the area, the total number of innovations per m2 was calculated. By dividing the number of innovations per m2 by 2 L (the rate of application), the number of innovations per L of suspension was calculated. The results are shown below in Figures 3 to 6.

Results In Trial 1, the total number of innovations per L of suspension for the in vitro Sphagnum was 9,525. In Trial 1, the number of large innovations per L of suspension for the in vitro Sphagnum was 521.

In Trial 2, the total number of innovations per L of suspension for the in vivo Sphagnum was 8,312. In Trial 2, the number of large innovations per L of suspension for the in vivo 20 Sphagnum was 794.

In Trial 3, the total number of innovations per L of suspension for the unchopped in vivo Sphagnum was 6,067. In Trial 3, the number of large innovations per L of suspension for the 25 unchopped in vivo Sphagnum was 828.

The unchopped in vivo Sphagnum shows a significant drop in the number of innovations compared to the chopped in vivo Sphagnum, showing that the capitula inhibits the number of growing points along the stem. By chopping the Sphagnum into appropriate sizes, the number of innovations can be maximised.

Although the number of innovations of in vitro Sphagnum is larger than in vivo Sphagnum, the number of large innovations is much more in the in vivo trial. This shows that the in vivo Sphagnum becomes more robust and produces larger innovations which helps initial 35 establishment.

When considering the total establishment in terms of the number of innovations and the size of those innovations, the in vivo Sphagnum of Trial 2 shows a significant improvement over the in vitro Sphagnum of Trial 1 and the unchopped Sphagnum of Trial 3.

EXAMPLE 2

Number of fragments of Sphagnum Materials and Methods Trial 1 was in vitro Sphagnum taken immediately from the laboratory after 4 months of growth. Trial 2 was first generation in vivo Sphagnum harvested after 6 months of growth in a heated indoor greenhouse, where in vitro Sphagnum was applied to produce the in vivo Sphagnum. Trial 3 was second generation in vivo Sphagnum harvested after 6 months of growth in a heated indoor greenhouse, where the first generation in vivo Sphagnum was harvested after 6 months of growth in a heated indoor greenhouse and applied to produce the second generation in vivo Sphagnum. Samples of 1 g of Sphagnum were harvested. Water content was standardised by compressing the Sphagnum with a force corresponding to 16 g / cm2 to provide a standardised mass per volume. Three samples were taken for Trial 1, and five samples were taken for Trial 2 and 3.

The samples were floated in water and all fragments having a length of at least 5 mm were selected for counting. The remaining material was strained in a sieve and removed with tweezers for weighing.

The weight of the fragments having a length of at least 5 mm was measured, and the number of fragments was counted. The weight of the fragments of less than 5 mm was also measured. The weight was standardised by draining until no more water drained by gravity, and then weighing. The proportion of fragments having a length of at least 5 mm by weight for each sample was then calculated. This process was repeated for each sample in each trial. The mean and standard deviations for each trial were then calculated. The number of fragments having a length of at least 5 mm per L of suspension was then calculated for each trial by multiplying the average number of fragments counted per sample and multiplying by 100 to provide the number of fragments per 100 g (approximately per L of suspension). The results are shown in Figures 7 to 9.

Results In Trial 1, the mean proportion of fragments of at least 5 mm by weight for the in vitro Sphagnum was 57%.

In Trial 2, the mean proportion of fragments of at least 5 mm by weight for the first generation in vivo Sphagnum was 48%.

In Trial 3, the mean proportion of fragments of at least 5 mm by weight for the second generation in vivo Sphagnum was 77%.

In Trial 1, the mean number of fragments of at least 5 mm per L of suspension for the in vitro Sphagnum was 6,633.

In Trial 2, the mean number of fragments of at least 5 mm per L of suspension for the first generation in vivo Sphagnum was 4,360.

In Trial 3, the mean number of fragments of at least 5 mm per L of suspension for the 15 second generation in vivo Sphagnum was 3,840.

EXAMPLE 3

Lengths of fragments of Sphagnum Materials and Methods Trial 1 was in vitro Sphagnum taken immediately from the laboratory after 4 months of growth. Trial 2 was first generation in vivo Sphagnum harvested after 6 months of growth in a heated indoor greenhouse, where in vitro Sphagnum was applied to produce the in vivo Sphagnum. Trial 3 was second generation in vivo Sphagnum harvested after 6 months of growth in a heated indoor greenhouse, where the first generation in vivo Sphagnum was harvested after 6 months of growth in a heated indoor greenhouse and applied to produce the second generation in vivo Sphagnum. Samples of approximately 1 g of Sphagnum were harvested. Water content was standardised by compressing the Sphagnum with a force corresponding to 16 g / cm2 to provide a standardised mass per volume. Three samples were taken for each of the trials.

The samples were floated in water and all fragments having a length of at least 5 mm were selected for counting.

The lengths of the fragments of at least 5 mm in length in each sample were measured by using a fine scale steel ruler. The mean length of the fragments for each sample was then calculated. The mean length of the fragments for each trial was then calculated. The results are shown in Figures 10 to 12.

Results In Trial 1, the mean length of fragments of at least 5 mm for the in vitro Sphagnum was 10.7 mm.

In Trial 2, the mean length of fragments of at least 5 mm for the first generation in vivo Sphagnum was 8.3 mm.

In Trial 3, the mean length of fragments of at least 5 mm for the second generation in vivo Sphagnum was 15.7 mm.

EXAMPLE 4

Diameters of stems of Sphagnum Materials and Methods Trial 1 was in vitro Sphagnum taken immediately from the laboratory after 4 months of growth. Trial 2 was first generation in vivo Sphagnum harvested after 6 months of growth in a heated indoor greenhouse, where in vitro Sphagnum was applied to produce the in vivo Sphagnum. Trial 3 was second generation in vivo Sphagnum harvested after 6 months of growth in a heated indoor greenhouse, where the first generation in vivo Sphagnum was harvested after 6 months of growth in a heated indoor greenhouse and applied to produce the second generation in vivo Sphagnum. Samples of approximately 100 g of Sphagnum were harvested. Ten sample strands were selected at random from these for each of the trials.

The diameter of the stems of the strands in each sample were measured. The stem diameter is measured as the thickness of the stem, preferably perpendicular to the length of the stem.

A microscope was used to measure the diameter of the stem in pixels, and this was converted into a diameter is mm. The microscope used has a standard magnification of 449 pixels per mm. In other cases, such as for larger diameters, this can be measured by using a length measuring tool such as callipers. Three measurements were taken over the length of each strand. The mean diameter for each sample was then calculated by summing the measurements and dividing by the number of measurements (three).

The mean diameter of the stems for each trial was then calculated. The mean can thus be calculated by summing the average diameter of each sample and dividing the total sum by the number of samples (ten).

The results are shown in Figures 13 to 15.

Results In Trial 1, the mean diameter of fragments for the in vitro Sphagnum was 0.27 mm.

In Trial 2, the mean diameter of fragments for the first generation in vivo Sphagnum was 0.49 mm.

In Trial 3, the mean diameter of fragments for the second generation in vivo Sphagnum was 0.48 mm.

The mean diameter increased significantly between the in vitro Sphagnum and the first generation in vivo Sphagnum. This demonstrates the rapid increase is stem thickness due to cultivating in vivo. The increase in stem thickness produces a much more robust fragment that is more adapted for water and nutrient uptake and water holding capacity, and provides for more effective initial establishment.

It can also be seen that the stem diameter changes insignificantly between the first generation and the second generation in vivo suspensions. Once the Sphagnum has undergone the first period of in vivo growth, the stems obtain a sufficient diameter, and do not change significantly with further growth.

EXAMPLES

Weight of fragments of Sphagnum Materials and Methods Trial 1 was in vitro Sphagnum taken immediately from the laboratory after 4 months of growth. Trial 2 was first generation in vivo Sphagnum harvested after 6 months of growth in a heated indoor greenhouse, where in vitro Sphagnum was applied to produce the in vivo Sphagnum. Trial 3 was second generation in vivo Sphagnum harvested after 6 months of growth in a heated indoor greenhouse, where the first generation in vivo Sphagnum was harvested after 6 months of growth in a heated indoor greenhouse and applied to produce the second generation in vivo Sphagnum. Three samples were taken for each of the trials.

The wet weight of the sample of fragments was measured. The water content was standardised by compressing the Sphagnum to a standard mass per volume by applying a force of 16 g / cm2 and allowing drainage.

The dry weight of the sample of fragments was then measured. The dry weight is defined as the weight of the Sphagnum when no more water can be removed. This was performed by drying the Sphagnum at 25 °C in a humidity of less than 50% until no further weight loss was recorded. In other examples, the Sphagnum can be heated at 110 °C for 24 hours.

The percentage water content of the samples was then calculated by dividing the wet weight by the dry weight. The results are shown in Figures 16 to 18.

Results In Trial 1, the mean dry weight of fragments for the in vitro Sphagnum was 1.43 g. The mean percentage water content was 98 %.

In Trial 2, the mean dry weight of fragments for the first generation in vivo Sphagnum was 2.95 g. The mean percentage water content was 97 °/0.

In Trial 3, the mean dry weight of fragments for the second generation in vivo Sphagnum was 3.06 g. The mean percentage water content was 97 %.

EXAMPLE 6

Distance between branches Materials and Methods Each of the three trials involved samples of first generation in vivo Sphagnum harvested after 6 months of growth in a heated indoor greenhouse, where in vitro Sphagnum was applied to produce the in vivo Sphagnum. Each of the trials used Sphagnum palustre. Five sample stems were taken for each trial.

The distance between branches on each stem was measured using callipers to the nearest 0.5 mm. At least six measurements were taken on each stem. The measurements were started approximately 1 cm below the capitula The results are shown in Figures 19 to 21.

Results In Trial 1, the average distance between branches was 5.27 mm.

In Trial 2, the average distance between branches was 4.95 mm.

In Trial 3, the average distance between branches was 5.00 mm.

Overall, the average across each trial is 5.08 mm. This supports providing fragment sizes of at least about 5 mm to provide a branch as a potential innovation.

Claims

CLAIMS: 1. A method of providing a seedstock of Sphagnum, comprising: providing in vitro Sphagnum; applying the Sphagnum to a growth surface, cultivating the Sphagnum in vivo on the growth surface; harvesting the cultivated Sphagnum from the growth surface; and chopping the harvested Sphagnum to provide a seedstock of Sphagnum for cultivation, the seedstock comprising a plurality of fragments of the in vivo Sphagnum.
2. The method according to claim 1, wherein the fragments of in vivo Sphagnum have a mean stem diameter which is at least 50% thicker than a mean stem diameter of the in vitro Sphagnum.
3. The method according to claim 1 or 2, wherein the fragments of in vivo Sphagnum have a mean stem diameter of at least 0.4 mm, and wherein the in vitro Sphagnum has a mean stem diameter of less than 0.3 mm.
The method according to any preceding claim, wherein the fragments of in vivo Sphagnum have a mean length of between 5 and 50 mm.
5. The method according to any preceding claim, wherein the providing in vitro Sphagnum comprises chopping in vitro Sphagnum into a plurality of fragments of in vitro Sphagnum.
The method according to claim 5, wherein the providing in vitro Sphagnum comprises mixing the plurality of fragments of in vitro Sphagnum with a first fluid solution to provide a suspension of in vitro Sphagnum.
7 The method according to claim 6, wherein the first fluid solution comprises water and a thickening agent dissolved in the water.
8. The method according to claim 6 or 7, wherein the suspension of in vitro Sphagnum comprises at least 50 g of in vitro Sphagnum per L of the first fluid solution.
The method according to any of claims 6 to 8, wherein the applying comprises applying the suspension of in vitro Sphagnum onto the growth surface at a density of between 0.5 and 3 L / m2.
10. The method according to any of claims 6 to 9, wherein the applying comprises spraying the suspension of in vitro Sphagnum onto the growth surface.
11. The method according to claim 10, wherein the spraying comprises pumping the suspension of in vitro Sphagnum through an aperture to hit a deflecting plate.
12. The method according to any preceding claim, wherein the growth surface comprises compost, soil, sand, peat, and/or dried Sphagnum.
13. The method according to any preceding claim, wherein the cultivating is carried out under controlled environmental conditions, wherein the controlled environmental conditions comprise at least one of: irrigation, nutrient supply, lighting, shading, temperature, and/or humidity.
14. The method according to any preceding claim, wherein the cultivating comprises irrigating the Sphagnum, comprising applying water at a rate of at least 1 L / m2 / day.
15. The method according to any preceding claim, wherein the cultivating comprises applying nutrients to the Sphagnum.
16. The method according to any preceding claim, wherein the cultivating is carried out for at least one month.
17. The method according to any preceding claim, wherein the chopping the harvested Sphagnum comprises chopping such that at least 50 % by weight of the fragments of in vivo Sphagnum have a length of at least 5 mm.
18. The method according to any preceding claim, wherein the providing the seedstock of Sphagnum comprises mixing the plurality of fragments of in vivo Sphagnum with a second fluid solution to provide a suspension of in vivo Sphagnum.
19. The method according to claim 18, wherein the second fluid solution comprises water and a thickening agent dissolved in the water.
20. The method according to any preceding claim, further comprising applying the seedstock of Sphagnum to a growth surface.
21. The method according to claim 20, further comprising cultivating the Sphagnum of the seedstock in vivo on the growth surface.
22. The method according to claim 21, further comprising harvesting the Sphagnum of the seedstock from the growth surface.
23. The method according to claim 22, further comprising providing a growing medium comprising the harvested Sphagnum of the seedstock, comprising drying the harvested Sphagnum of the seedstock.
24. The method according to claim 22, further comprising chopping the harvested Sphagnum from the seedstock to provide a second seedstock of Sphagnum for cultivation, the second seedstock comprising a plurality of fragments of second generation in vivo Sphagnum.
25. Sphagnum obtainable by the method according to any one of the preceding claims.