GB2575576A - De novo synthesized combinatorial nucleic acid libraries - Google Patents

De novo synthesized combinatorial nucleic acid libraries Download PDF

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Publication number
GB2575576A
GB2575576A GB1914881.6A GB201914881A GB2575576A GB 2575576 A GB2575576 A GB 2575576A GB 201914881 A GB201914881 A GB 201914881A GB 2575576 A GB2575576 A GB 2575576A
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United Kingdom
Prior art keywords
polynucleotides
nucleic acid
variant
library
encodes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB1914881.6A
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English (en)
Other versions
GB201914881D0 (en
Inventor
Cox Anthony
Chen Siyuan
Ledogar Charles
Toppani Dominique
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Twist Bioscience Corp
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Twist Bioscience Corp
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Publication date
Application filed by Twist Bioscience Corp filed Critical Twist Bioscience Corp
Publication of GB201914881D0 publication Critical patent/GB201914881D0/en
Publication of GB2575576A publication Critical patent/GB2575576A/en
Withdrawn legal-status Critical Current

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    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B35/00—ICT specially adapted for in silico combinatorial libraries of nucleic acids, proteins or peptides
    • G16B35/10—Design of libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Library & Information Science (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Management, Administration, Business Operations System, And Electronic Commerce (AREA)
GB1914881.6A 2017-03-15 2018-03-14 De novo synthesized combinatorial nucleic acid libraries Withdrawn GB2575576A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762471723P 2017-03-15 2017-03-15
US201762578326P 2017-10-27 2017-10-27
PCT/US2018/022487 WO2018170164A1 (en) 2017-03-15 2018-03-14 De novo synthesized combinatorial nucleic acid libraries

Publications (2)

Publication Number Publication Date
GB201914881D0 GB201914881D0 (en) 2019-11-27
GB2575576A true GB2575576A (en) 2020-01-15

Family

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GB1914881.6A Withdrawn GB2575576A (en) 2017-03-15 2018-03-14 De novo synthesized combinatorial nucleic acid libraries

Country Status (11)

Country Link
US (1) US20180282721A1 (https=)
EP (1) EP3596258A4 (https=)
JP (2) JP7335165B2 (https=)
KR (2) KR102607157B1 (https=)
CN (2) CN110914486B (https=)
AU (2) AU2018234624B2 (https=)
CA (1) CA3056386A1 (https=)
GB (1) GB2575576A (https=)
IL (1) IL269288A (https=)
SG (1) SG11201908489XA (https=)
WO (1) WO2018170164A1 (https=)

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SG11201600853UA (en) 2013-08-05 2016-03-30 Twist Bioscience Corp De novo synthesized gene libraries
US10669304B2 (en) 2015-02-04 2020-06-02 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
EP3350314A4 (en) 2015-09-18 2019-02-06 Twist Bioscience Corporation OLIGONUCLEIC ACID VARIANT LIBRARIES VARIANT AND SYNTHESIS THEREOF
KR102794025B1 (ko) 2015-09-22 2025-04-09 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
EP3500672A4 (en) 2016-08-22 2020-05-20 Twist Bioscience Corporation DE NOVO SYNTHETIZED NUCLEIC ACID LIBRARIES
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
GB2573069A (en) 2016-12-16 2019-10-23 Twist Bioscience Corp Variant libraries of the immunological synapse and synthesis thereof
EP4556433A3 (en) 2017-02-22 2025-08-06 Twist Bioscience Corporation Nucleic acid based data storage
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
AU2018234624B2 (en) * 2017-03-15 2023-11-16 Twist Bioscience Corporation De novo synthesized combinatorial nucleic acid libraries
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231872A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US11407837B2 (en) 2017-09-11 2022-08-09 Twist Bioscience Corporation GPCR binding proteins and synthesis thereof
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
KR102804057B1 (ko) 2018-01-04 2025-05-07 트위스트 바이오사이언스 코포레이션 Dna 기반 디지털 정보 저장
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
WO2020139871A1 (en) 2018-12-26 2020-07-02 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
CN113766930B (zh) 2019-02-26 2025-07-22 特韦斯特生物科学公司 Glp1受体的变异核酸文库
JP2022522668A (ja) 2019-02-26 2022-04-20 ツイスト バイオサイエンス コーポレーション 抗体を最適化するための変異体核酸ライブラリ
US11332738B2 (en) 2019-06-21 2022-05-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
JP2022548783A (ja) 2019-09-23 2022-11-21 ツイスト バイオサイエンス コーポレーション 単一ドメイン抗体のバリアント核酸ライブラリー
EP4034566A4 (en) 2019-09-23 2024-01-24 Twist Bioscience Corporation VARIANT NUCLEIC ACID BANKS FOR CRTH2
BR112022011235A2 (pt) 2019-12-09 2022-12-13 Twist Bioscience Corp Bibliotecas de variantes de ácido nucleico para receptores de adenosina
MX2022013499A (es) 2020-04-27 2023-01-16 Twist Bioscience Corp Bibliotecas de ácidos nucleicos variantes para coronavirus.
EP4204460A4 (en) 2020-08-26 2024-09-25 Twist Bioscience Corporation METHODS AND COMPOSITIONS RELATING TO GLP1R VARIANTS
CN117043171A (zh) 2020-08-28 2023-11-10 特韦斯特生物科学公司 用于合成的装置和方法
EP4229210A4 (en) 2020-10-19 2025-01-08 Twist Bioscience Corporation METHOD FOR THE SYNTHESIS OF OLIGONUCLEOTIDES USING BOUND NUCLEOTIDES
KR20230147617A (ko) 2021-01-21 2023-10-23 트위스트 바이오사이언스 코포레이션 아데노신 수용체에 관한 방법 및 조성물
EP4314075A4 (en) 2021-03-24 2025-04-09 Twist Bioscience Corporation VARIANT NUCLEIC ACID BANKS FOR CD3
WO2022235584A1 (en) 2021-05-03 2022-11-10 Twist Bioscience Corporation Variant nucleic acid libraries for glycans
US12201857B2 (en) 2021-06-22 2025-01-21 Twist Bioscience Corporation Methods and compositions relating to covid antibody epitopes
WO2023023285A2 (en) 2021-08-19 2023-02-23 Twist Bioscience Corporation Methods and compositions relating to covalently closed nucleic acids
US12134656B2 (en) 2021-11-18 2024-11-05 Twist Bioscience Corporation Dickkopf-1 variant antibodies and methods of use
EP4460516A2 (en) 2022-01-03 2024-11-13 Twist Bioscience Corporation Bispecific sars-cov-2 antibodies and methods of use
US20230340461A1 (en) * 2022-02-14 2023-10-26 Twist Bioscience Corporation Combinatorial dna assembly for multispecific antibodies
US20230325641A1 (en) * 2022-04-07 2023-10-12 Winbond Electronics Corp. Light source optimization apparatus and light source optimization method
CN114694757B (zh) * 2022-04-20 2025-04-18 中南民族大学 Rna序列编码潜力预测方法及系统
WO2025217152A1 (en) * 2024-04-09 2025-10-16 Octant, Inc. Methods and systems for identifying functional sites
WO2025250626A2 (en) * 2024-05-28 2025-12-04 Immorna Biotherapeutics Inc. Mrna composition and uses thereof

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US20110082055A1 (en) * 2009-09-18 2011-04-07 Codexis, Inc. Reduced codon mutagenesis
WO2014035693A2 (en) * 2012-08-31 2014-03-06 The Scripps Research Institute Methods and compositions related to modulators of eukaryotic cells
US20150038373A1 (en) * 2013-08-05 2015-02-05 Twist Bioscience Corporation De novo synthesized gene libraries
WO2017049231A1 (en) * 2015-09-18 2017-03-23 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof

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US20080287320A1 (en) * 2006-10-04 2008-11-20 Codon Devices Libraries and their design and assembly
US20110082055A1 (en) * 2009-09-18 2011-04-07 Codexis, Inc. Reduced codon mutagenesis
WO2014035693A2 (en) * 2012-08-31 2014-03-06 The Scripps Research Institute Methods and compositions related to modulators of eukaryotic cells
US20150038373A1 (en) * 2013-08-05 2015-02-05 Twist Bioscience Corporation De novo synthesized gene libraries
WO2017049231A1 (en) * 2015-09-18 2017-03-23 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof

Also Published As

Publication number Publication date
JP2023087685A (ja) 2023-06-23
CA3056386A1 (en) 2018-09-20
KR20230163591A (ko) 2023-11-30
AU2018234624A1 (en) 2019-10-17
JP7335165B2 (ja) 2023-08-29
KR20190129081A (ko) 2019-11-19
US20180282721A1 (en) 2018-10-04
JP2020511135A (ja) 2020-04-16
EP3596258A4 (en) 2020-12-30
CN117888207A (zh) 2024-04-16
JP7696937B2 (ja) 2025-06-23
CN110914486A (zh) 2020-03-24
GB201914881D0 (en) 2019-11-27
AU2024201012A1 (en) 2024-06-06
IL269288A (en) 2019-11-28
SG11201908489XA (en) 2019-10-30
CN110914486B (zh) 2024-01-30
EP3596258A1 (en) 2020-01-22
WO2018170164A1 (en) 2018-09-20
KR102607157B1 (ko) 2023-11-27
AU2018234624B2 (en) 2023-11-16

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